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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for 7 Bromo 2 methylimidazo 4 5 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... cleared by centrifugation at 1500rpm for 5 minutes at 4⁰C and then passed through a 0.45um pore PVDF Millex-HV (Millipore). Lentivirus was aliquoted and stored at −80⁰C for future use.
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was centrifuged in a Beckman JA25.50 rotor at 16,000 rpm for 30 min at 4°C and 8.4 mL 5% neutralized PEI (Sigma P3143) was added dropwise into the supernatant with gentle agitation for 15 minutes at 4°C followed by another centrifuge at 13,000 rpm for 10 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... and membrane blocked overnight while shaking at 4°C in 5% milk (Marvel) containing 0.077% sodium azide (Sigma Aldrich). Primary antibodies were diluted in 5% milk/PBS/0.1% TWEEN 20 at concentrations as follows ...
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bioRxiv - Plant Biology 2021Quote: ... Pellet was washed twice with acetone at maximum speed (13,000xg) for 5 minutes at 4◦C and then air dried and resuspended in 50 mM ammoniumbicarbonate (ABC) (Sigma).
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated at 37 °C and 5% CO2 for 4 days before addition of resazurin (Sigma Aldrich) to a final concentration of 20 μg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... Dehydrated tissue was then bleached with gentle shaking at 4°C for 48 hours in 1.5 ml of 5% H2O2 (Sigma) in methanol (1 volume of 30% H2O2 in 5 volumes of 100% methanol ...
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bioRxiv - Microbiology 2021Quote: ... centrifuged for 5 min at 16,000 g at 4°C and homogenized with glass beads (≤ 106 μm; G4649, SIGMA) in 350 μl of lysis buffer (RNeasy Plus Mini Kit ...
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bioRxiv - Cancer Biology 2020Quote: ... Conditioned media were retrieved by spinning down at 2,000 × g for 5 minutes at 4 °C then supernatants were passed through 0.22 μm filter (Millipore: SLGP033RS).
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bioRxiv - Molecular Biology 2021Quote: ... The unbroken cells were removed by centrifugation (3,000 x g; 4°C; 5 min; Sigma 3-16KL; rotor 11180). The membrane fraction was pelleted down by high-speed centrifugation (100,000 x g ...
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bioRxiv - Biophysics 2019Quote: ... The eluate was incubated for 3 h at 4°C with 5 mL of FLAG M2 Agarose beads (Sigma), collected in a glass column ...
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bioRxiv - Immunology 2022Quote: ... overnight at 37°C in 5% CO2 tissue culture incubator and were subsequently fixed with 4% paraformaldehyde (Sigma-Aldrich) in DPBS for 30 minutes at RT ...
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bioRxiv - Molecular Biology 2019Quote: ... 1000 rpm continuous shaking at 4 °C) with 100 μL elution buffer (6.7 mM m6A 5-monophosphate sodium salt [Sigma # M2780-10MG] ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular ions were extracted from dried pellets by overnight incubation at 4°C in 5 ml milliRho water (Millipore). Na+ and K+ were measured by flame spectroscopy with a Solaar spectrometer.
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bioRxiv - Immunology 2019Quote: ... Cells were blocked overnight at 4°C with 150µl blocking solution per well (5% (v/v) human serum (Sigma), 5% (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell disruption was carried out by vortexing (3 cycles, 5 min each) at 4°C using 0.2 ml of glass beads (425-600 μm; Sigma). For the Rpb3 immunopurification ...
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bioRxiv - Microbiology 2022Quote: ... 4 °C), resuspended in 5 ml lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5ξCellLytic B (Sigma-Aldrich), 1 % Triton X-100 ...
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bioRxiv - Neuroscience 2023Quote: ... Immunoprecipitation was carried out by rotating sonicated lysates overnight at 4 C with NFIA antibody (5 mg, Sigma, HPA006111) or SOX9 antibody (EMD Millipore ...
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bioRxiv - Systems Biology 2022Quote: ... The lysate was centrifuged at 4,000 xg for 5 min at 4°C and the protein pelletwas resuspended in Digestion Buffer(10% 2,2,2-Trifluoroethanol (Sigma; #96924)) in 100 mM HEPEs pH 8.5) ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was cleared by centrifugation at 16,000 g for 5 min at 4 °C before it was immobilized on anti-FLAG (Sigma) agarose bead slurry for 30 min at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and then incubated overnight at 4°C with primary antibodies diluted in blocking solution [5% Goat Serum (Sigma Aldrich) + 0.2% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: Membranes were incubated overnight in primary antibody o/n at 4°C: Rabbit ⍺-Vang (Rawls & Wolff, 2003) diluted 1:1,000 in 5% milk in TBST or Rabbit ⍺-Actin (Sigma) diluted 1:2,000 in 10% Seablock in TBST followed by 3x wash in TBST for 10 minutes each ...
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bioRxiv - Synthetic Biology 2024Quote: ... washed once with TBS pH 8.0 for 5 min and incubated for overnight at 4 °C in primary antibody (Mouse-anti-FLAG M2 [Sigma #F1804 ...
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bioRxiv - Cell Biology 2023Quote: ... pelleted by centrifugation (5 min, 400 gavg, 4°C) and washed in phosphate buffered saline (PBS, Sigma, D8537-500ml), before being pelleted again ...
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bioRxiv - Biochemistry 2024Quote: ... Transferred PVDF membranes were blocked in 5% milk powder in 0.2% Tween-TBS overnight at 4°C and immunoblotted with mouse anti-FLAG-HRP (Sigma) conjugated primary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were counterstained with DAPI (4′,6-diamidino-2-phenylindole dihydrochloride; 5 µg/ml, Sigma-Aldrich) at room temperature for 20 minutes.
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bioRxiv - Neuroscience 2022Quote: ... Sections were mounted after 4′,6-diamidino-2-phenylindole (DAPI) staining (1:5 000, Sigma-Aldrich, USA). Confocal images were captured under 20× objective using A-1R Confocal Microscope (Nikon ...
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bioRxiv - Cell Biology 2021Quote: ... The parasites (ring stage; 2-4 hpi) were tightly synchronized with 5% (v/v) D-sorbitol (Sigma) and then monitored by Giemsa staining of methanol-fixed blood smears ...
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bioRxiv - Neuroscience 2022Quote: ... and ≥99% 4-methyl-3-heptanol and 99% 6-methyl-5-hepten-2-one from Sigma-Aldrich (Item numbers M48309 and M48805-100ML ...
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bioRxiv - Cell Biology 2023Quote: ... the DNA was stained with 5 μg/ml 4′,6-diamidino-2-phenylindole (DAPI, D9542-5MG, SIGMA) diluted in 2% bovine serum albumin (BSA ...
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bioRxiv - Physiology 2019Quote: ... 8 bromo-cGMP (cGMP)37 (Sigma-Aldrich, Oakville, ON, Canada). Unstimulated controls consisted of tubules bathed in physiological saline with no diuretic application ...
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bioRxiv - Molecular Biology 2021Quote: ... and 26 mM NaHCO3 and 98 µM bromo-deoxyuridine (Sigma), and pre-plated for 1.5 hours at 37 °C to obtain a cardiomyocyte-rich fraction ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 µL of 1-bromo-3-chloropropane (Sigma-Aldrich B9673) were added (100 µL per 1 mL TRI reagent ...
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bioRxiv - Microbiology 2020Quote: ... samples in TRIzol were separated using Bromo-chloro-propane (Sigma). The aqueous phase was collected and glycogen was added as a carrier ...
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bioRxiv - Microbiology 2021Quote: ... samples in TRIzol were separated using Bromo-chloro-propane (Sigma). The aqueous phase was collected ...
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bioRxiv - Microbiology 2020Quote: ... samples in TRIzol were separated using Bromo-chloro-propane (Sigma). The aqueous phase was collected and glycogen was added as a carrier ...
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bioRxiv - Genomics 2022Quote: ... 40 μl of 1-bromo-3-chloropropane (BCP) (Sigma, B9673) was added to the samples and samples were vortexed for 20 sec ...
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bioRxiv - Genomics 2022Quote: ... 1-bromo-3-chlorophenol (100 μL; Sigma-Aldrich, Taufkirchen, Germany) was added ...
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bioRxiv - Molecular Biology 2022Quote: ... treated twice using 1-Bromo-3-chloropropane (Sigma-Aldrich, B9673) followed by RNA precipitation using 2-propanol (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... 50 μL of 1-bromo-3-chloro-propane (Sigma-Aldrich) was added ...
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bioRxiv - Cell Biology 2022Quote: ... 2-hydroxy-4’-(2- hydroxyethoxy)-2-methylpropiophenone (I2959) (Sigma-Aldrich, USA), a photoinitiatior (0.5% w/v ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 2’,7’-Dichlorofluorescin diacetate (H2DCFDA) were procured from Sigma-Aldrich, USA ...
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bioRxiv - Cell Biology 2021Quote: ... 1µM 4-hydroxy 5-methytetrahydrofolate (SIGMA).
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bioRxiv - Molecular Biology 2022Quote: ... IL-4 (5 ng/ml; Sigma) 0.5 μg/ml of anti-CD180 (RP105 ...
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bioRxiv - Immunology 2023Quote: ... 4 μl betaine (5 M, Sigma), 0.12 μl MgCl2 (1 M ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-OHT (5 µM; Sigma-Aldrich), Dp44mT (5 μM) ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 ng/mL IL-4 (Sigma), and 0.5 ug/mL anti-CD180 (BD Pharmingen ...
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bioRxiv - Neuroscience 2022Quote: ... and Ara-C (2 uM, Sigma) to inhibit glial proliferation ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and cultured overnight at 37 °C in 5% CO2 in neurobasal medium supplemented with 2% B27 (Sigma-Aldrich) and containing 50 ng/mL GDNF (PeproTech ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated with 0.1 mL serially diluted supernatant containing SARS-CoV-2 for 30 minutes at 37°C and 5% CO2 before being left in 1.5mL overlay (DMEM, 2% FBS, 1.2% cellulose microcrystalline (Sigma)) at 37°C and 5% CO2 for 72 hours ...
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bioRxiv - Bioengineering 2020Quote: ... and MEF cells fixed with 4°C 4% formaldehyde (Sigma-Aldrich, 252549) in PBS for 20 minutes at room temperature ...