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Citations for Millipore Sigma :
551 - 600 of 10000+ citations for 7 Bromo 2 methylimidazo 4 5 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The sections were counterstained with 5 μM 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI) (Sigma-Aldrich, Cat# D9542) in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 5 min with 10 μg/ml 4’,6-Diamidino-2-phenylindole (DAPI; Sigma-Aldrich) to stain cell nuclei ...
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bioRxiv - Genomics 2021Quote: ... 10 mM MgCl2,10 mM Tris pH 7.4, 1 mM CaCl2) with increasing amounts (0, 0.5, 1, 2, 5, 7.5, 10 U) of MNase (Sigma) at 30°C for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... and 3 μg/mL + 7 μg/mL FRD+U (5-Fluoro-2′-deoxyuridine, Sigma-Aldrich, Cat# F0503 and Uridine, Sigma-Aldrich, Cat# U3003-5G). 120 µL of cell suspension was carefully plated onto each coverslip and allowed to adhere for 2 hours at 37°C and 5% CO2 ...
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The heterogeneity of the DNA damage response landscape determines patient outcomes in ovarian cancerbioRxiv - Cell Biology 2021Quote: ... washed in sterile 4°C dPBS (D8537, Sigma), pelleted again and dPBS was removed ...
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bioRxiv - Microbiology 2023Quote: ... 4 °C in 10 kDa Amicon (Merck Millipore) filters ...
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bioRxiv - Neuroscience 2024Quote: ... Ara-C (4 mM; Sigma Aldrich, catalog# C6645), 10 ng/mL of BDNF ...
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bioRxiv - Neuroscience 2019Quote: ... 7-Nitroindazole (7-Ni, Sigma) was dissolved in DMSO and used at 100 and 200uM ...
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bioRxiv - Synthetic Biology 2023Quote: ... 100 μL of 1– bromo–3–chloropropane (Sigma Aldrich, B9673-200ML) was added to the cell lysate ...
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bioRxiv - Bioengineering 2023Quote: ... Phase separation was performed with 1-bromo-3-chloropropane (Sigma Aldrich) and the aqueous phase was purified using RNeasy micro spin columns ...
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bioRxiv - Developmental Biology 2023Quote: ... A volume of 200 μl 1-bromo-3-chloropropane (Sigma Aldrich) was added and the mixture was incubated for 10 min at room temperature before centrifugation (10 min at 10.000 x g) ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were bulk-fixed at 4°C in 4% paraformaldehyde (Sigma-Aldrich 158127) for 24 h ...
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bioRxiv - Biophysics 2021Quote: We fixed cells with cold (+4°C) DMEM with 4% Paraformaldehyde (P6148, Sigma) for 15 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Cells were fixed overnight at 4°C with 4% methanol-free formaldehyde (Sigma) in PBS ...
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bioRxiv - Immunology 2023Quote: ... and digested overnight at 4°C in 4 mg/mL Dispase II (Sigma) while rotating ...
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bioRxiv - Cell Biology 2021Quote: African green monkey kidney Vero E6 cell and Colorectal Adenocarcinoma human epithelial (Caco-2) cells were maintained at 37 °C in 5% CO2 in Dulbecco’s minimal essential medium (DMEM) (Sigma) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated for 2 hours at 35 °C with 5 μl of 500 mM CaCl2 (Sigma-Aldrich), 250 ng of competence stimulating peptide 1 (CSP-1 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated at 37°C under 5% CO2 in serum free DMEM containing 2% penicillin/streptomycin (Sigma) and 1 µg/ml TPCK Trypsin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated at 37°C with 5% CO2 in serum free DMEM containing 2% penicillin/streptomycin (Sigma) and 1 µg/ml TPCK Trypsin (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... Around 2–5 μl of 2 mM levamisole (Sigma) was pipetted out on the center of the agarose pads ...
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bioRxiv - Cell Biology 2021Quote: ... 4-Chloro-2-Methylthiopyrimidine (Sigma, 145289), GW3965 (Sigma ...
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bioRxiv - Microbiology 2020Quote: Cells were incubated with 10 µM 2′,7′-dichlorofluorescein (DCFDA) (Sigma, USA) in PBS for 30 min in dark ...
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bioRxiv - Molecular Biology 2022Quote: ... serially diluted from 10-7 to 10-2 in PBS (Sigma®) and plated onto BHI agar plates ...
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bioRxiv - Cell Biology 2021Quote: ... and 2’,7’-dichlorofluorescin diacetate (DCF-DA) (35845) were purchased from Sigma. Guinea pig anti-rabbit IgG (Heavy & Light Chain ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2’,7’ dichlorofluorescein diacetate (DCFH-DA) were procured from Sigma-Aldrich (USA). We purchased the nitro blue tetrazolium (NBT ...
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bioRxiv - Biophysics 2023Quote: ... and ultrapure H2O and finally incubated in a solution of poly-T ssDNA (100 nM) in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (10 mM HEPES, NaCl 50 mM, pH 7, Sigma-Aldrich, St Louis, MO, USA) for 1 h at approx ...
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bioRxiv - Cancer Biology 2019Quote: ... 5-chloro-2’-deoxyuridine (CIdU) and 5-lodo-2’-deoxyuridine (ldU) were obtained from Sigma.
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bioRxiv - Neuroscience 2023Quote: Subcutaneous injections of 5-Chloro-2’-deoxyuridine (CldU) or 5-Iodo-2’deoxyurdine (IdU) (Sigma) were administered (50μg/g dissolved in saline ...
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bioRxiv - Immunology 2023Quote: ... 3-4 and 7-9 were treated with 100 µL of embryo water plus 1 µg/mL 2′,7′-Dichlorofluorescein diacetate (H2DCFDA) (Sigma-Aldrich) in 0.4% DMSO ...
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bioRxiv - Plant Biology 2023Quote: ... 2 mM Ca(NO3)2 ×24H2O (13477-34-4, Sigma), 2.5 mM KH2PO4 (7778-77-0 ...
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bioRxiv - Plant Biology 2021Quote: Dried samples were resuspended in 75 μL of 0.2M of methoxyamine hydrochloride in pyridine (Sigma), incubated at 60°C for 45 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Dried samples were derivatised with 20 µl methoxyamine (30 mg/ml in pyridine, Sigma Aldrich) and 20 µl N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA ...
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bioRxiv - Cancer Biology 2022Quote: ... Dried samples were derivatised with 20 µl methoxyamine (30 mg/ml in pyridine, Sigma Aldrich) and 20 µl N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA ...
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bioRxiv - Cell Biology 2023Quote: ... samples were incubated overnight in 20µl of 20mg/ml methoxyamine hydrochloride in pyridine (Sigma Aldrich). The next day ...
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bioRxiv - Cell Biology 2019Quote: 5-7 day old adult females were fed on 0,5% Methylene Blue (Sigma) dissolved in 85% heat-killed yeast paste for 5 hours at 25°C secondary to 5 hours starvation ...
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bioRxiv - Neuroscience 2019Quote: ... supernatant discarded and spheres digested for 5-7 min in Accumax solution (Sigma). With the help of a pipette ...
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bioRxiv - Cell Biology 2021Quote: ... for 5-7 minutes and neutralized with trypsin neutralization solution (Sigma, Cat. #T9253). Neurons were then counted and plated onto Laminin-treated coverslips (Neuvitro ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing PelXC232S were pooled and concentrated to a volume of 2 mL by centrifugation at 2 200 × g at a temperature of 4 °C using an Amicon ultra centrifugal filter device (Millipore) with a 10 kDa molecular weight cut-off ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed on 2 mg of total protein by incubating 2h at 4°C with anti-Flag agarose beads (Sigma). Beads were then washed in lysis buffer and bound polypeptides analyzed by SDS-PAGE and immunoblotting ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were subsequently incubated in blocking buffer containing 0.1% Tween 20 and the appropriate primary antibodies for 1-2 hr at room temperature or 4°C overnight: rabbit anti-Myc (1:2,000, Sigma, C3956), mouse anti-FLAG (1:2,000 ...
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bioRxiv - Biophysics 2020Quote: ... The virus-containing supernatant was concentrated by ultracentrifugation in a SW-28 rotor (25,000 rpm, 2 h, 4°C) using OptiPrep density gradient medium (Sigma-Aldrich). Virus containing fractions were collected ...
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bioRxiv - Cell Biology 2021Quote: ... Cdc5-3V5 and Rim4-3V5 (control non-kinase tagged protein) were immunoprecipitated at 4°C 2 hours using 20 µL of anti-V5-agarose slurry (Sigma). Purifications were washed 4 times with NP40 buffer.
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bioRxiv - Biophysics 2021Quote: ... The virus-containing supernatant was concentrated by ultracentrifugation in a SW-28 rotor (25,000 rpm, 2 h, 4°C) using OptiPrep density gradient medium (Sigma-Aldrich). Part of the supernatant containing pelleted viruses was collected ...
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bioRxiv - Biochemistry 2022Quote: ... membranes were equilibrated at 4 °C and incubated for 30 min in the presence of 2 mg/ml iodoacetamide (Sigma). Membranes were then solubilized in 10 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... supernatant was collected in clean microcentrifuge tube and incubated 2 h at 4°C with a-flag mouse antibody (Sigma), following 1 h incubation at 4°C with PureProteomeProtein A/G Magnetic Beads Mix (Millipore) ...
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bioRxiv - Physiology 2021Quote: ... was incubated for 2-3 h at 4 °C with 30 µl of FLAG-conjugated beads (anti-DYDDDDDK beads, Sigma) or empty beads (ThermoScientific) ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysate was immunoprecipitated for 2 hours at 4°C with the following antibodies: rabbit anti-myc (2.5 μg, Millipore Sigma), mouse anti-HA (2.5 μg ...
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bioRxiv - Neuroscience 2020Quote: ... 0.3% TritonX/PBS) for 2 hours and stained with primary antibodies overnight at 4°C (guinea pig anti-vGlut2, 1:1000, Millipore AB2251-I ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... membranes were blocked for 1 h at RT in 5% skimmed milk powder and 0.2% Tween in PBS before incubating overnight at 4◦C with the primary antibodies mouse anti-TH (1:1000, Millipore) and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Plant Biology 2020Quote: ... stratified for 2 d at 4°C in the dark and sown on Gamborg’s B5 Basal Salt medium (Sigma G5768) supplemented with 1% (w/v ...