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Citations for Millipore Sigma :
3501 - 3550 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and the samples were washed 3 times for 5 min in PBS and counter-stained with 1:1000 DAPI (D9542, Sigma) for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Beads were then washed four times with cold lysis buffer and immune complexes were eluted with 75 μL of 3XFLAG peptide (Sigma) (200 mg/mL ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... Other cell wall pellet was washed three times with 150 mM NaCl and extracted twice with a 1 M NaOH containing 1 mg/mL BH4Na (Sigma), at 10-20 mg of cell wall/mL at 65°C for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed three times with 1x PBS and then permeabilized with 0.5%Triton-×100/PBS (Triton-X 100: Sigma, T8787) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed 3 times in 0.01% PBS-Tx (1xPBS with 0.01% Triton X-100 (Sigma-Aldrich, Cat#X100)) for 10 mins at RT ...
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bioRxiv - Biochemistry 2024Quote: ... washed one time with 40 ml cold 1 M sorbitol and resuspended in a Ficoll buffer (18% Ficoll type 400 (Sigma), 20 mM KH2PO4 pH 6.8 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The wells were washed four more times and 200 μL of OPD (o-phenylenediamine dihydrochloride) substrate (P8412 Sigma-Aldrich, France) was added in each well ...
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bioRxiv - Microbiology 2024Quote: ... the membrane was washed three times with TBS-T before incubating with mouse monoclonal anti-FLAG coupled to HRP (#A8592, Sigma) at a 1:5,000 titer in TBS-T ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were washed three times with PBS and nuclei were stained with DAPI (0.5 µg/ml, Sigma-Aldrich, D8417-10MG) for 10 min on RT ...
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bioRxiv - Microbiology 2022Quote: ... Glucose kit: Glucose (HK) Assay Kit (Sigma, product Code GAHK-20). Citric acid kit ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies fused to HRP were used for detection (Goat anti-mouse HRP 1:3000, BioRad; Goat anti-rat HRP 1:3000, Merck Millipore; Goat anti-rabbit HRP 1:3000, BioRad)
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bioRxiv - Cell Biology 2020Quote: ... The PCR fragments generated were cloned into a pET-41 Ek/LIC vector (Novagen) expressing an N-terminally GST-His6-tagged protein ...
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bioRxiv - Microbiology 2021Quote: ... which was combined with 10µL of KiCqStart SYBR Green PCR Ready Mix (Millipore Sigma), 1µL of forward primer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The PCR products were purified using PCRu96™Plates (Merck Millipore Inc., Burlington, MA) and sequenced by an ABI 3130 (Applied Biosystems ...
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bioRxiv - Developmental Biology 2020Quote: ... qRT-PCR was performed with SYBR Green Jump-start Taq Ready-mix (Sigma, S4438) on the Mx3005P qPCR System (Agilent Technologies Genomics) ...
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bioRxiv - Developmental Biology 2020Quote: ... Q-PCR was performed with SYBR Green Jump-start Taq Ready-mix (Sigma, S4438) on the Mx3005P qPCR System (Agilent Technologies Genomics ...
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bioRxiv - Neuroscience 2019Quote: ... The wild-type or T94M mutant ORFs were then PCR-cloned into pET28c (Novagen) for bacterial production of the proteins with an N-terminal 6× His tag ...
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bioRxiv - Cancer Biology 2021Quote: ... The PTBP1 ORF was PCR amplified and subcloned by gibson cloning into pET21A+ (Novagen) digested by XhoI and NheI ...
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bioRxiv - Biochemistry 2021Quote: ... PCR products were separated by electrophoresis on a 2.5% agarose gel containing GelRed (Sigma).
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bioRxiv - Biochemistry 2020Quote: ... The resulting PCR fragment and the Plasmid DNA of the overexpression vector pET28a(+) (Novagen) were digested with NdeI and BamHI ...
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bioRxiv - Developmental Biology 2019Quote: ... qRT-PCR was performed using the SYBR Green JumpStart Taq Ready Mix (Sigma Aldrich) on an ABI Prism 7900 system (Applied Biosystems) ...
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bioRxiv - Synthetic Biology 2020Quote: ... All PCR reactions were carried out by KOD Hot Start DNA polymerase (Merck Millipore). PCR and Gel purification were done using Qiaquick nucleotide removal kit (qiagen ...
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bioRxiv - Genomics 2020Quote: ... we added 11 µl of KAPA2G Robust PCR master-mix (Sigma Catalogue No. KK5005), comprising 4 µl KAPA2G buffer ...
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bioRxiv - Microbiology 2020Quote: ... A second round of PCR performed with KOD Hot Start Master Mix (Millipore, 71842) supplemented with 1mM MgCl2 under the following parameters ...
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bioRxiv - Microbiology 2020Quote: ... All inverse PCR reactions were carried out using KOD hot-start polymerase (EMD Millipore) according to manufacturer’s instructions ...
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bioRxiv - Paleontology 2019Quote: ... Bisulfite treated DNA were amplified with the FastStart High Fidelity PCR System (Sigma, 03553400001) using the primers listed in Extended Data Table 12 ...
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bioRxiv - Immunology 2021Quote: ... PCR amplicons were cleaned up using SpeedBeads™ magnetic carboxylate (obtained from Sigma Aldrich). The size of barcoded PCR products (approximately 1500 bp ...
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bioRxiv - Developmental Biology 2021Quote: ... qRT-PCR was performed with the SYBR Green JumpStart Taq Ready Mix (Sigma – S4438) and custom-made primers (Table S6 ...
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bioRxiv - Immunology 2020Quote: ... The PCR products were sub-cloned into the plasmid expression vector pET-28a (Novagen) previously cut with the restriction enzymes EcoR I and Xho I ...
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bioRxiv - Bioengineering 2022Quote: ... and (ii) inlets of the hydrogel chamber were sealed using PCR tape (Sigma Aldrich). Depending on the type of analysis ...
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bioRxiv - Biochemistry 2022Quote: ... The sequence was verified by colony PCR using standard primers (Sigma-Aldrich, Taufkirchen, Germany) and Taq polymerase (In house ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product was detected by electrophoresis on agarose gel (Sigma-Aldrich, Darmstadt, Germany), using ethidium bromide (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR products were transformed into pKM208 carrying Rosseta 2 (Millipore Sigma 71400-3) strain by electroporation ...
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bioRxiv - Immunology 2022Quote: ... ORFs were synthesized as gBlocks (IDT) and assembled using PCR (KOD mastermix, Milipore Sigma) using the following primers ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting supernatant was used for genotyping through REDTaq PCR Reaction Mix (Sigma Aldrich). Specific primer sets were applied to detect the CMV-Cre and IL-17A transgenes ...
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bioRxiv - Biophysics 2024Quote: ... 45 μL of proteinase K (PK) mix (20 μg PK (Sigma Aldrich, PCR grade), 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... amplified with the primers P2 and P3 (S2 Table) by KOD PCR (Sigma-Aldrich). Transfected parasites were selected in medium containing mycophenolic acid (25 μg/ml ...
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bioRxiv - Neuroscience 2023Quote: Fragments of eaat genes were PCR amplified using a JumpStart Taq polymerase (Sigma-Aldrich) and the primer pairs eaat5b_135_s 5’-GGAGCAGGAAGTCAAGTA-3’ with eaat5b_600_as 5’-GTCCGTCCCATTATCGTC-3’ and eaat7_142_s 5’-GTAATAGCAGGCACAGTGATG-3’ with eaat7_1001_as 5’-CCCAGAGCAGTGATCCAAG-3’ ...
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bioRxiv - Biochemistry 2022Quote: ... The DNA test sequences for cyclization experiments (bottom PCR templates) were synthesized by Sigma Genosys (Israel) ...
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bioRxiv - Cell Biology 2023Quote: ... MPP6 and NO66 were PCR cloned from plasmid DNA or cDNA into pFLAG (Sigma), pEF6 (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... The genes of interest were PCR amplified using JumpStart REDTaq ReadyMix (Sigma-Milipore, P0982) according to the manufacturer’s protocols ...
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bioRxiv - Genetics 2023Quote: ... specific primers were designed and PCR was performed using KOD DNA polymerase (Sigma Aldrich). The amplification of DNA fragments was done following manufacturer’s instructions into a Bioer GeneExplorer thermal Cycler ...
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bioRxiv - Cell Biology 2023Quote: ... cDNAs were mixed with gene specific primers and SYBR green PCR MasterMix (Sigma Aldrich) and run on an Applied Biosystems 7900HT Fast Real-Time PCR system.
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bioRxiv - Genetics 2023Quote: ... qRT-PCR was performed with the SYBR Green JumpStart Taq Ready Mix (Sigma – S4438) and custom-made primers (Table S4 ...
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bioRxiv - Genetics 2023Quote: ... The PCR product was purified using Amicon Ultra-2mL 50K centrifugal filter (Millipore UFC205024) to exchange the PCR solution for 1X NEB CutSmart buffer ...
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bioRxiv - Microbiology 2024Quote: ... Donor DNA was amplified by PCR using the high fidelity KOD DNA polymerase (Novagen) to amplify a fragment containing the tag and the resistance cassette ...
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bioRxiv - Immunology 2024Quote: ... The PCR reaction was carried out by KAPA2G Fast ReadyMix™ (Sigma Aldrich, KK5103). Primers were designed targeting the intron 1 and intron 3 regions of the target DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were blocked with 10% FBS in PBS for 1 h at RT and incubated overnight at 4°C with a primary anti-γH2AX antibody (05-636, EMD Millipore, 1:500 in 10% FBS-PBS). Following primary antibody incubations ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 3x PBS washes for 5 minutes at RT before blocking with blocking buffer (BB; 5% normal donkey serum Sigma-Aldrich #S30-M, 3% BSA (Sigma #A4503) and 0.25% Triton-X100 (Sigma #T9284 ...
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bioRxiv - Cell Biology 2020Quote: ... were reconstituted in ACN/IPA/H2O (65:30:5, v/v/v, 100 µL) by vortexing (2 × 30 s, RT) and transferred to an amber vial (Sigma; QSertVial™, 12 × 32 mm, 0.3 mL) for LC–MS analysis.