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Citations for Millipore Sigma :
3851 - 3900 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were then washed three times with TBST and incubated with peroxidase-conjugated anti-mouse IgG secondary antibody (1:4,000 dilution) (Sigma-Aldrich; catalog no. A4416) at room temperature for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing rabbit anti-TH antibody (1:1000; EMD Millipore, Burlington, MA) with gentle agitation at 4°C for 44-48 hr ...
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bioRxiv - Neuroscience 2023Quote: ... The organoids were washed three times for 15 minutes with 1x PBS (Roth, Cat. no. 1105.1) and then incubated in 30% sucrose (Sigma Aldrich, Cat. no. S7903) in PBS solution at 4°C until they sunk to the bottom of the dish ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing rabbit anti DsRed polyclonal antibody (1:1000; EMD Millipore, Burlington, MA) with gentle agitation at 4°C for 18-22 hr ...
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bioRxiv - Biophysics 2023Quote: ... The mass of the oligonucleotides was determined using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS, Bruker, AutoFlex Max) using 2’,6’-dihydroxyacetophenone (DHAP, Sigma, Cat. No. 37468) as a matrix ...
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bioRxiv - Cell Biology 2023Quote: ... PDMS substrates were rinsed with sterile water three times and coated with 50 µg/mL of fibronectin (F4759, Sigma-Aldrich, St. Louis, MO) diluted in water for one hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike-pseudotyped viruses were then concentrated 10 times at 2000×g using 100 kDa cut-off Amicon Ultra-15 Centrifugal Filter Units (Millipore. Cat. No. UFC910024).
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... dehydrated through a series of increasing concentrations of ethanol and cleared three times for 5 minutes in xylene (Sigma Aldrich, cat. no. 247642). At the end ...
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bioRxiv - Cell Biology 2023Quote: ... and keloid scar tissue were washed several times with phosphate-buffered saline (PBS, pH 7.4) and incubated with 2.4 units of Dispase® (Sigma, St. Louis, MO, USA) for 12 h ...
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bioRxiv - Cell Biology 2023Quote: ... The beads were washed four times with 500 µl of Binding Buffer and then eluted with 2X Sample Buffer (Millipore Sigma #S3401-10VL). The total and bound fractions were then analyzed via Western blotting using standard conditions ...
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bioRxiv - Bioengineering 2023Quote: ... resuspended in citrate-PBS and washed 4 times in citrate-PBS supplemented with 0.01% bovine serum albumin (BSA, 10735086001, Sigma-Aldrich, Zwijndrecht, The Netherlands) to remove all Lymphoprep ...
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bioRxiv - Genetics 2023Quote: ... The samples were collected for a range of incubation times and then examined by western blotting using an anti-His antibody (Sigma, catalogue no. SAB1305538) or anti-GFP (Roche ...
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Juxtacrine DLL4-NOTCH1 signaling between astrocytes drives neuroinflammation via the IL-6-STAT3 axisbioRxiv - Neuroscience 2023Quote: ... the pellet was washed 3 times with the buffer B and filtered through a 100 µm nylon mesh (Millipore Corporation, Burlington, MA, USA). The nylon mesh was washed with 7 mL of buffer B to collect the retained enriched neurovascular fractions ...
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bioRxiv - Molecular Biology 2023Quote: ... the two were mixed gently at a ratio of 1:2 (v/v) and resuspended in 20 times ice-cold Collagen (Sigma, Cat # C0130-1G). Cells were then aliquoted in 20 µl volumes into a 48-well plate and allowed to polymerize at 37□°C for 45Lmin ...
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bioRxiv - Genetics 2023Quote: ... The resulting SNCs were purified by washing with ultrapure water three times and concentrated with Amicon® Ultra Centrifugal Filters (Millipore Sigma, USA). ATRA was conjugated onto SNC-PEG-NH2 via EDC/NHS catalyzed amidation ...
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bioRxiv - Microbiology 2024Quote: ... this culture was incubated for a further 1 h before washing three times and RNA was extracted using Triazole (Sigma Aldrich, England, UK) following manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were washed three times in 0.4% PBST before mounting on glass slides in mounting medium (Mowiol 4-88 anti-fade agent, EMD Millipore Corp., Billerica, MA). The following primary antibodies were used ...
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bioRxiv - Cell Biology 2020Quote: Glomerular DNA was used for PCR using the RedTaq Ready MasterMix (Sigma-Aldrich, St. Louis, USA) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were treated with RED extract-N-Amp Tissue PCR reagents (Millipore Sigma, Saint-Louis, MO) to purify genomic DNA and perform the genotyping PCR ...
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bioRxiv - Biophysics 2022Quote: ... sub-cloned into the pNKS2 vector) via PCR with custom-designed primers (Eurofins Genomics, Sigma-Aldrich) and PfuUltra II Fusion HS DNA polymerase (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR reactions were run under standard conditions using KOD Hot Start Master Mix (Sigma-Aldrich: 71842). After subsequent ligation with T4 ligase (NEB ...
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bioRxiv - Developmental Biology 2019Quote: ... and then diluted 1:10 for subsequent qRT-PCR analysis using SYBR Green JumpStart Taq (Sigma) on a CFX Connect Real-Time PCR machine (Biorad) ...
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bioRxiv - Biochemistry 2020Quote: ... cerevisiae DNA the coding sequence for Reb1 was amplified by PCR and cloned into pET21b (Novagen) via InFusion cloning (Clontech ...
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bioRxiv - Biochemistry 2020Quote: ... The PCR reaction was carried out using LiTIMFP and LiTIMRP (Table S1. Sigma-Aldrich Co, India) as the forward and the reverse primers ...
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bioRxiv - Synthetic Biology 2021Quote: ... the truncated AtCCD7 gene was amplified by PCR and cloned into the pCDFDuet-1 plasmid (Novagen) using NcoI and NotI to yield the plasmid pCDFDuet-trAtCCD7 ...
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bioRxiv - Systems Biology 2021Quote: ... 15 µL from each PCR product were mixed with 5 µL 6x loading dye solution (Sigma) before being loaded onto a 2% (w/v ...
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bioRxiv - Microbiology 2019Quote: ... Genes or loci of interest were PCR amplified with KOD Xtreme Hot Start polymerase (Millipore Sigma) using the primers listed in Table S6 ...
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bioRxiv - Paleontology 2019Quote: 3µl of bisulfite treated DNA were amplified with the FastStart High Fidelity PCR System (Sigma, 03553400001) using the primers listed in Extended Data Table 12 ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting PCR products were ligated into the same sites in plasmid pET-22b(+) (Novagen). An identical strategy was used to generate plasmids pET-YpTatB22-220 and pET-YpTatB22-91 for expressing and purifying full-length or truncated versions of TatB from Y ...
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bioRxiv - Neuroscience 2021Quote: ... DNA-free isolation was verified by PCR amplification (RedTaq® Polymerase, catalog no. D4309, Sigma-Aldrich). For this purpose ...
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bioRxiv - Microbiology 2022Quote: ... After amplification PCR products were checked on 1% (w/v) agarose gel (Sigma-Aldrich, Darmstadt, Germany) (5 µL of each PCR product mixed with 1 µL 6x loading dye for 50 min at 120 V ...
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bioRxiv - Plant Biology 2024Quote: ... PCR-grade H2O and RedTaq Ready Mix according to the manufacturer’s instructions (Sigma-Aldrich, Rehovot, Israel). The amplified products were subjected to electrophoresis on 1.2% agarose gel containing agarose in 0.5 X TBE buffer and were stained with ethidium bromide ...
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bioRxiv - Microbiology 2024Quote: ... The second PCR products were cleaned up using SpeedBeads™ magnetic carboxylate (obtained from Sigma Aldrich). Cleaned PCR2 products were quantified using Qubit® dsDNA HS Assay Kit (Life Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... The PCR product was then mixed with 1/3 volume 10 M ammonium acetate (Sigma, A1542) and 2 volume ethanol (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... DNA fragments encoding human Kid and mNeonGreen were amplified by PCR and assembled into pFastbac1 (Novagen) by Gibson assembly as described (42) ...
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bioRxiv - Genomics 2023Quote: ... The amplified cDNA was purified using the HighPrep PCR Clean-up System (Sigma Aldrich AC-60050) adding a volume of magnetic beads equal to 0.8x the sample volume ...
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bioRxiv - Synthetic Biology 2023Quote: ... Splicing by overlap extension PCR was performed with the KOD Xtreme Hot Start DNA Polymerase (Novagen). Generally ...
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bioRxiv - Cell Biology 2023Quote: TRPM8 sequences were amplified by PCR using the following primers (ordered from Millipore Sigma, Burlington, MA):
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bioRxiv - Microbiology 2023Quote: ... Each 20 µl PCR reaction contained 10 µl of JumpStart RedTaq DNA Polymerase Master Mix (Sigma), 8 µl dH2O ...
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bioRxiv - Microbiology 2023Quote: ... PCRs were performed in 25 µl with JumpStart RED Accutaq LA polymerase (Sigma-Aldrich, cat# D8045) in the supplied buffer ...
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bioRxiv - Microbiology 2024Quote: ... PCR mixture was composed of 7.5 µL of JumpStart™ REDTaq® ReadyMix™ (Sigma-Aldrich), 0.75 µL of each primer 10 µM (515F and R926[24]) ...
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bioRxiv - Immunology 2024Quote: ... 80µl of plasma per sample was plated in a 96-well PCR plate (Sigma Aldrich #EP951020401), frozen ...
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bioRxiv - Genomics 2021Quote: ... The total number of cells in each field was determined by counterstaining cell nuclei with 4,6-diamidine-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich; 50 mg/ml in PBS for 15 min at RT). Immunostained cells were mounted in Aqua-Poly/Mount (Polysciences ...
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bioRxiv - Developmental Biology 2024Quote: ... The total number of cells in each field was determined by counterstaining cell nuclei with 4,6-diamidine-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich; 50 mg/ml in PBS for 15 min at RT). Immunostained cells were analyzed via confocal microscopy using a Nikon A1R+ ...
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bioRxiv - Genomics 2022Quote: ... The total number of cells in each field was determined by counterstaining cell nuclei with 4,6-diamidine-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich; 50 mg/ml in PBS for 15 min at RT). To improve the efficiency of Tbr2 detection ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei extraction was performing using the Nuclei Isolation Kit: Nuclei PURE Prep Kit (Millipore Sigma) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Synthetic Biology 2019Quote: We used the commercial kit: myTXTL from Arbor Biosciences (Sigma 70 Master Mix Kit, (USA). We used both our plasmid (10 nM final concentration ...
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bioRxiv - Plant Biology 2020Quote: ... and purified with a commercial kit according to manufacturer’s instructions (His-bind buffer kit, Millipore).
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bioRxiv - Physiology 2022Quote: ... cells were washed two times with PBS+/+ and medium was replaced with glucose-free DMEM (catalog n°D5030, Sigma-Aldrich, Saint-Louis, MO, USA) without phenol red supplemented with 2 mM L-glutamine ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were then washed three times in 1× PBST prior to mounting on glass slides in glycerol with Mowiol 4-88 anti-fade agent (EMD Millipore Corp., Billerica, MA). When required ...