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Citations for Millipore Sigma :
3401 - 3450 of 4935 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Individual expression constructs were subsequently transformed into Escherichia coli Rosetta™ 2 (DE3)pLysS cells (Novagen) for heterologous expression ...
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bioRxiv - Cell Biology 2023Quote: MFF-ΔTM was expressed in RosettaTM2 BL21-(DE3) Escherichia coli (71400; EMD Millipore Corporation, Burlington, MA) in LB broth ...
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bioRxiv - Biochemistry 2023Quote: MFF-ΔTM was expressed in RosettaTM2 BL21-(DE3) Escherichia coli (71400; EMD Millipore Corporation, Burlington, MA) in LB broth ...
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bioRxiv - Molecular Biology 2023Quote: ... coli were adapted to grow in 2.5 mM paraquat (methyl viologen dichloride hydrate, Sigma Aldrich 856177) following a modified adaptive evolution protocol 52,53 ...
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bioRxiv - Biophysics 2023Quote: ... Subsequently the expression vector was transformed into and overexpressed in Escherichia coli BL21 (DE3) cells (Novagen). The recombinant WT and mutant hSOD1 proteins were all found in inclusion body ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids were transformed into Escherichia coli BL21(DE3) CodonPlus cells and grown in Terrific Broth (Sigma) at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... 1 μg/mL Escherichia coli lipopolysaccharide (M1 polarizing positive control; Sigma-Aldrich, Cat. No. L3024-5mg), 20 ng/mL mouse IL-4 (M2 polarizing positive control ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli was grown in LB media (LB broth, Miller) with kanamycin (Millipore Sigma, 50 µg/mL).
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1 µg/ml of a SARS-CoV nucleocapsid protein (NP) monoclonal antibody (mAb) 1C7C7 (Millipore-Sigma, St. Louis, MO) shown to cross-react with SARS-CoV-2 NP ...
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bioRxiv - Immunology 2022Quote: Stock concentration of VSV-SARS-CoV-2 at a concentration of ~150 µg/mL viral RNA was conjugated with Atto565-NHS ester (Sigma-Aldrich, cat.72464) as previously described (32 ...
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bioRxiv - Immunology 2020Quote: ... a pooled rhesus serum collected day 14 after infection with SARS CoV-2 was used as standard after depletion of IgG using GE Healthcare Protein G Sepharose (Sigma, St. Louis, MO) as described (52) ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1 µg/ml of a SARS-CoV nucleocapsid protein (NP) monoclonal antibody (mAb) 1C7C7 (Millipore-Sigma, St. Louis, MO) shown to cross-react with SARS-CoV-2 NP ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells were infected with a MOI 0.002 (250 plaque forming units (PFU)) of SARS-CoV-2 for 1hr before overlaying with 1% methylcellulose- viscosity (4,000 centipoises) (Sigma- Aldrich, St. Louis, MO). Cells were incubated for 4 days at 37°C before fixing in 8% formaldehyde and stained with 1% crystal violet to visualize plaques ...
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bioRxiv - Microbiology 2023Quote: hPCLS were infected with SARS-CoV-2 at 2×105 TCID50 per slice and treated with homoharringtonine (HHT) (Sigma, cat# SML-1091-10MG) at 1 h p.i ...
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bioRxiv - Cell Biology 2021Quote: ... 5 × 104 cells were used per poly-L-lysine (1:100 dilution with bidest, Millipore, # A-003-E) coated 10 mm coverslip ...
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bioRxiv - Neuroscience 2019Quote: ... P0 rat hippocampal neurons were plated to poly-D-lysine (PDL; 10μg/cm2; Millipore Cat# A-003-E) coated coverslips at 5×104 neurons per well in a 24-well plate ...
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bioRxiv - Cell Biology 2020Quote: ... and chamberslides were in additions pre-treated with Poly-D-Lysine (Cat nb A-003-E, Millipore, Sigma) to increase adherence of the ECM ...
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bioRxiv - Cell Biology 2020Quote: ... and chamberslides were in additions pre-treated with Poly-D-Lysine (Cat nb A-003-E, Millipore, Sigma) to increase adherence of the ECM ...
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bioRxiv - Microbiology 2021Quote: ... E2 (CHIK-102 and CHIK-263) [42] or Zika Virus E as a control (ZV-2, Sigma-Aldrich) (kindly provided by Dr ...
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bioRxiv - Microbiology 2021Quote: The anti-flavivirus E monoclonal antibody (mAb) D1-4G2-4-15 was purchased from Millipore (Billerica, MA, USA). The rabbit anti-ZIKV E polyclonal antibody was purchased from GeneTex (Alton PkwyIrvine ...
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bioRxiv - Cell Biology 2020Quote: ... The primers for PKC isoforms and E-cadherin were adopted from Awadelkarim et al.iv and Labernadie et al.v and ordered from Sigma. Prior to all experiments ...
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bioRxiv - Cell Biology 2020Quote: ... Spread screen samples were infected with VACV E-EGFP L-mCherry at MOI 0.02 and infection media was changed to media containing AraC (10µM, Sigma) at 8 hpi.
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bioRxiv - Genetics 2020Quote: ... 20 mM MgSO4, 200 mM HEPES, pH 7.9), protease inhibitor cocktail (AEBSF, 1,10-Phenanthroline, Pepstatin A, E-64) (Sigma), and TritonX-100 ...
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bioRxiv - Biochemistry 2020Quote: ... 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and α-tocopherol (vitamin E) was from Sigma Aldrich (St. Louis, MO, USA). TNF-α was obtained from Invitrogen (Carlsbad ...
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bioRxiv - Cancer Biology 2024Quote: Formalin fixed paraffin embedded tissues were routinely stained with hematoxylin-eosin (H&E) (Sigma-Aldrich, St. Louis, MO), and immunohistochemistry was performed for E-cadherin and vimentin ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were incubated in 1mL of Protease solution composed of 7.5mg/mL of protease E (P8811, Sigma Aldrich) diluted in dissociation medium [6mM CaCI2 ...
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bioRxiv - Developmental Biology 2023Quote: ... The zona pellucidae of the GV zygotes and cleavage-stage embryos were removed with 0.5% pronase E (Sigma) and the cleavage-stage embryos were then incubated in Ca2+-free Chatot-Ziomek-Bavister (CZB ...
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bioRxiv - Cell Biology 2023Quote: ... 4 x 105 cells per well were plated in media containing 100 nM GSI (Compound E; Millipore, 565790) on non-tissue-culture treated 6-well plates that were pre-treated overnight with PBS + 0.1 mg/ml Poly-D-lysine (Thermo Scientific ...
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bioRxiv - Biophysics 2024Quote: ... E-cadherin knockdown was induced in shCDH1 cells by adding 200 μM isopropyl-β-D-thiogalactoside (IPTG; Sigma) 72 h prior to experiments.
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bioRxiv - Cell Biology 2023Quote: ... this was done by incubating embryos right after fertilization in 1.5 mg/mL Pronase E (Sigma-Aldrich, #107433) for approximately five minutes ...
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bioRxiv - Bioengineering 2023Quote: dPPM was stained with H&E (Hematoxylin solution, Gill no.2, Sigma Aldrich, aqueous Eosin Y, Thermo scientific) to assess cellular content and histoarchitecture ...
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bioRxiv - Physiology 2023Quote: ... sectioned and stained with hematoxylin and eosin (H&E) or immunohistochemically stained for Tyrosine Hydroxylase (ab152, Millipore Sigma) or UCP1 (ab10983 ...
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bioRxiv - Immunology 2023Quote: ... Routine hematoxylin and eosin (H&E) stainings and Periodic Acid Schiff (PAS) staining (Millipore-Sigma, catalog #395B-1KT) were performed per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Routine hematoxylin and eosin (H&E) stainings and Periodic Acid Schiff (PAS) staining (Millipore-Sigma, catalog #395B-1KT) were performed per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... E-A14 and M-A14 cells and 10µg.mL−1 of Insulin-transferrin-sodium selenite media supplement (Sigma-Aldrich) for NEU15.
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bioRxiv - Cancer Biology 2023Quote: ... Sections of 4 µm were stained with haematoxylin and eosin (H&E, Sigma-Aldrich, St. Louis, MO, EUA) or antibodies against fibronectin (ab2413 ...
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bioRxiv - Pathology 2023Quote: Hair follicle parameters were stained with H&E (H3136 Sigma-Aldrich Hematoxylin and E4009 Sigma-Aldrich Eosin Y). Dermis-dermal adipocyte layer thickness and the numbers of hair follicles and anagen stages were counted in three areas (9 sections ...
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bioRxiv - Cell Biology 2023Quote: ... 300,000 to 400,000 NPCs seeded into microscope coverslips were treated with 100nM compound E (EMD Millipore, Temecula, CA) in BrainPhys medium (StemCell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... The signal was detected by the Pro e-BLOT Touch Imager using chemiluminescent substrate (WBKLS0500; Merck Millipore, Boston), and the band intensity was measured using ImageJ software (National Institutes of Health).
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bioRxiv - Bioengineering 2021Quote: ... The digging movements of the valves induced displacement of the beam and this displacement was imaged using a camera (Panasonic DC-S1 with Sigma 70 mm f/2.8 DG Macro lens). The generated force is considered a point load ...
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bioRxiv - Plant Biology 2020Quote: ... 200 μl of N,O-Bis(trimethylsilyl)trifluoroacetamide+1% trimethylsilyl (BSTFA+1% TMCS, Sigma) were added and incubated at 110 °C for 15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Histones were extracted from nuclei by treatment with 0.4 N H2SO4 (Sigma 258105-500mL) for 30 minutes at room temperature and then precipitated from the supernatant by dropwise addition of ice-cold trichloroacetic acid (Sigma T069-100mL) ...
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bioRxiv - Cell Biology 2020Quote: ... containing 50 µM phenylmethysulfonyl fluoride (PMSF) and 50 µM N-methylmaleimide (all Sigma-Aldrich), then sonicated and 10% v/v β-mercaptoethanol added (Sigma-Aldrich) ...
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bioRxiv - Physiology 2022Quote: ... and glucose-6-phosphatase enzyme (catalog n°G8404, Sigma-Aldrich, Saint-Louis, MO, USA).
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bioRxiv - Neuroscience 2019Quote: ... we also applied 5 mM QX-314 (Lidocaine N-ethyl bromide; Millipore Sigma, Canada) via the intracellular recording solution in order to block sodium (Salazar et al. ...
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bioRxiv - Neuroscience 2019Quote: ... we conducted intraperitoneal (IP) injection or cannula infusion of Clozapine-N-Oxide (CNO, Sigma) to inactivate infected neurons or terminals before behavioral testing ...
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bioRxiv - Developmental Biology 2019Quote: ... S4 are anti human Tcf7l2 (N-20, SCBT) and anti-gamma tubulin (T9026, Sigma) HRP coupled secondary antibodies (1/2,000 ...
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bioRxiv - Neuroscience 2019Quote: Female fly heads (10 per N) were collected and homogenized in RIPA buffer (Sigma). 4X SDS loading buffer was added ...
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bioRxiv - Microbiology 2019Quote: ... 3-(N-Morpholino) propanesulfonic acid (MOPS) was obtained from Sigma Aldrich (St. Louis, MO). YPD broth medium and YPD agar were obtained from Becton ...
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bioRxiv - Cancer Biology 2019Quote: ... Control extracts were also incubated with 50 mM N-Ethyl Maleimide (NEM; Sigma-Aldrich) to inhibit any UbL conjugation ...