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Citations for Millipore Sigma :
3501 - 3550 of 4935 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... the samples were transferred to a wash-N-dry coverslip rack (Z688568, Sigma-Aldrich) for further processing steps in glass beakers of 100 mL capacity ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 50 µl of plasma were mixed with N-(2- Mercaptopropionyl)glycine (tiopronin, Sigma-Aldrich) solution to reach a final concentration of 100 mM and incubated for 30 minutes at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... REC114 variants were cloned as N-terminal Strep-tag fusions into pRSFDuet-1 (Novagen) and co-expressed with MEI41-127 that was cloned as a His-tag fusion into pProEXHTb (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... Antibodies were produced and biotinylated in house using (+)-Biotin N-hydroxysuccinimide ester (Sigma Aldrich) in bicarbonate buffer ...
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bioRxiv - Bioengineering 2023Quote: ... we incubated samples in Ce3D clearing solution containing 22% (v/v) N-methylacetamide (Sigma), 80% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... 20µl of N,O-bis(trimetylsilyl)trifluoroacetamide (BSTFA) and 1% trimethylchlorosilane (TMCS) (Sigma Aldrich) was added and samples were incubated at room temperature for at least one hour before GC-MS analysis.
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bioRxiv - Cell Biology 2023Quote: ... and 0.33 mM MgSO4) with 0.2 mM N-Phenylthiourea (PTU, Sigma-Aldirch cat # P7629), from 24 hpf onwards and under standard laboratory conditions ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were further derivatized with 10 µL tert-butyldimethylsilyl-N-methyltrifluoroacetamide (Sigma; product 394882) and incubated at 70°C for 60 minutes.
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bioRxiv - Developmental Biology 2023Quote: ... DNA was extracted from individual blastocysts using the Red Extract-N-Amp kit (Sigma), in 36μl final volume ...
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bioRxiv - Biochemistry 2023Quote: ... or a VSVG epitope tag on the N-terminal tag of H2A (Sigma, V4888) and a fluorescently labelled secondary antibody (Cell Signaling ...
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bioRxiv - Developmental Biology 2022Quote: ... DNA was purified with RedExtract-N-Amp tissue PCR kit (XNAT-1000RXN, Sigma-Aldrich) using directions provided by the manufacturer ...
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bioRxiv - Bioengineering 2023Quote: ... Dibenzocyclooctyne-sulfo-N-hydroxysuccinimidyl (DBCO-NHS) ester was purchased from Sigma-Aldrich (cat #: 762040). Anhydrous dimethyl sulfoxide (DMSO ...
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bioRxiv - Bioengineering 2023Quote: ... n-butyllithium and MoS2 bulk were purchased from Sigma-Aldrich (St. Louis, MO, USA). Doxorubicin hydrochloride was obtained from TCI (Tokyo ...
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bioRxiv - Bioengineering 2023Quote: ... dissolved in 100 mM 2-(N-morpholino) ethanesulfonic acid (MES; Sigma-Aldrich, Schnelldorf, Germany) at pH 5 for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... were derivatized using methoxyamine hydrochloride (MOX) and N,O-Bis(trimethylsilyl)trifluoroacetamide (TMS) (Sigma). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... injection of 5 mg N-nitrosodiethylamine (DEN)/kg body weight (Sigma, St. Louis, MO) in sterile water at 15 days of age ...
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bioRxiv - Physiology 2023Quote: ... and resuspended in 500uL of N-Ethylmaleimide (NEM) (Millipore Sigma #E3876, Burlington, MA, USA) solution (2.5 mM NEM in 80% Methanol ...
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bioRxiv - Neuroscience 2023Quote: ... and homogenized sufficiently in ice-cold Nuclei EZ Lysis Buffer (Millipore Sigma, N-3408). The resulting suspension was incubated on ice for 5 min and filtered through a 70-μm strainer ...
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bioRxiv - Molecular Biology 2023Quote: SK-N-BE cells were cultured in RPMI (Sigma-Aldrich, Saint Louis, MO, USA) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were further derivatized by adding 10 μL tert-butyldimethylsilyl-N-methyltrifluoroacetamide (Sigma, 394882) and incubating at 70°C for 60 minutes.
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bioRxiv - Biophysics 2023Quote: ... they were incubated with NaOH 0.1M and (N-[3(Trimethoxysilyl)propyl]ethylenediamine) (Sigma Merck) for 3 minutes each step ...
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bioRxiv - Microbiology 2023Quote: ... Resuspended cells were split and incubated with either 1-N-phenylnaphthylamine (NPN, Sigma Aldrich) at a final concentration of 20 µM or propidium iodide (PI ...
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bioRxiv - Molecular Biology 2023Quote: ... SK-N-BE cells were exposed to 50 ng/mL Doxycycline (Sigma-Aldrich, #D9891) for 24h before the sodium arsenite treatment (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... purified RSP protein was mixed with Biotin-NHS (Biotinamidocaproate N-hydroxysuccinimide ester, Sigma Aldrich) and three rounds of MACS (with RSP concentrations of 100 nM (the first one ...
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bioRxiv - Neuroscience 2024Quote: ... made using injections of the excitotoxin n-methyl-D-aspartate (NMDA; 0.09M, Sigma-Aldrich) into the coordinates shown in Table 1 ...
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bioRxiv - Biophysics 2024Quote: ... 1.2 M betaine) and solubilised with 1% (w/v) n-Decyl-Beta-Maltoside (Sigma) at 4°C in the dark with constant stirring for 1 hour ...
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bioRxiv - Immunology 2024Quote: Mice (n=40) were administered an intraperitoneal injection of pristane (0.5 ml) (Sigma-Aldrich). Following 4 weeks of pristane treatment ...
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bioRxiv - Cell Biology 2024Quote: ... The 24-well casting plate was pretreated O/N with 1% Pluronic acid (Sigma) and kept at 4 °C for 24 hours to prevent cell attachment ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... a primary antibody against the HCoV-OC43 N protein (Millipore #MAB9013; diluted 1:500) was used ...
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bioRxiv - Microbiology 2024Quote: ... Ecotin or PIC (positive control) and N-Methoxysuccinyl-Ala-Ala-Pro-Val-AMC (Sigma) 20µM as substrate ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were lysed in the presence of 50 mM N-ethylmaleimide (NEM) (Sigma) with lysis buffer containing 1% IGEPAL (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... GECs were additionally treated with N-acetyl Cysteine (NAC) (50 uM, Millipore Sigma, A9165) for 1 h and/or eATP (3mM ...
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bioRxiv - Synthetic Biology 2024Quote: ... cultures were supplemented with N-acetylneuraminic acid (neuraminic acid, Neu5Ac; Sigma, St. Louis, MO) at a 1 mM concentration unless otherwise indicated.
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bioRxiv - Biophysics 2021Quote: ... The His6-SUMO-C1Bδ fusion protein was expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma). For the natural abundance preparations ...
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bioRxiv - Cell Biology 2020Quote: ... coli BL21(DE3) in lysogeny broth (LB) media supplemented with 50 μg/ml kanamycin (Sigma-Aldrich, K1377). Expression was induced with 0.5 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2021Quote: ... coli were cultured at 30°C in Lysogeny Broth (LB) containing 30 μg/mL chloramphenicol (Sigma-Aldrich) until reaching an optical density (OD600 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... coli clones were grown at 37°C under aeration in liquid media Luria broth (LB) from SIGMA — or McConkey and LB agar plates ...
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bioRxiv - Physiology 2020Quote: ... coli strain and RNA expression was induced by 1 mM isopropyl-β-d-thiogalactopyranoside (IPTG, Sigma-Aldrich). We used a slightly modified version of the established RNAi-by-feeding protocol [81] ...
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bioRxiv - Microbiology 2019Quote: ... Escherichia coli was grown at 37°C in LB medium with 100 μg/mL ampicillin (Sigma-Aldrich) and 20 μg/mL chloramphenicol (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Escherichia coli O55: B5 Lipopolysaccharide (LPS 250 ng/mL, Cat. L2880, Sigma Aldrich, St. Louis, MO, USA), or Phorbol Myristate Acetate/Ionomycin (PMA 50 ng/mL ...
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bioRxiv - Cell Biology 2021Quote: ... The Bin1b-SH3 construct was transformed into Eschericia coli RosettaTM 2 (DE3) competent cells (Novagen, Merck, 71403) by heat shock ...
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bioRxiv - Biochemistry 2021Quote: ... coli TOP10F’ cells were plated on LB agar plates supplemented with 50 µg/mL kanamycin (Sigma-Aldrich). Single colonies were selected and inoculated in LB liquid medium supplemented with 50 µg/mL kanamycin ...
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bioRxiv - Molecular Biology 2020Quote: ... coli EF-G (704 aa, C-terminally His6-tagged) cloned into pET24a+ plasmid (Novagen, kanamycin resistance vector) was transformed into an E ...
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bioRxiv - Cell Biology 2020Quote: ... or lipopolysaccharide (LPS, 1 µg/ml, Escherichia coli O55:B5, Sigma-Aldrich, Merck, St. Louis, Missouri, USA). Afterwards ...
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bioRxiv - Microbiology 2022Quote: ... The B-subunit of Shiga toxin 1 (StxB) recombinantly produced in Escherichia coli was from Sigma Aldrich. LY294002 ...
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bioRxiv - Immunology 2022Quote: ... cells were treated with LPS (100 ng/ml) from Escherichia coli O111:B4 (Sigma, St. Louis, MO). For anti-inflammatory polarization DCs were treated with 1uM of dexamethasone (DEX ...
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bioRxiv - Microbiology 2019Quote: ... lipopolysaccharide from Escherichia coli (LPS) and Griess reagent were purchased from Sigma-Aldrich (St. Louis, MO, USA). The dichloro-dihydro-fluorescein diacetate (DCFH-DA ...
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bioRxiv - Bioengineering 2020Quote: ... we cultured Escherichia coli strain JM109 in 250-mL 2× Yeast extract Tryptone growth medium (Sigma Aldrich) with 5 mM MgCl2 (Sigma Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... coli strains expressing either RFP or GFP were separately cultured in Luria-Bertani (LB) medium (Sigma Aldrich) containing 1 µL/mL of chloramphenicol to retain the recombinant plasmid ...
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bioRxiv - Biochemistry 2021Quote: ... coli polar lipids (Avanti, US) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) (Sigma Aldrich) in a 3:1 molar ratio ...