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Citations for Millipore Sigma :
3551 - 3600 of 4935 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: GST and Recombinant Virp1-GST proteins were expressed in Escherichia coli Rosetta strain (EMD Millipore, Burlington, MA). Cells were grown overnight at 37°C in LB media supplied with ampicillin (0.1 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... coli BL21(DE3) strain and protein expression was induced in Overnight Express(tm) Instant TB Medium (Novagen) at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... coli B834(DE3) was grown in chemically defined medium (CDM) supplemented with 20 mg/ml SeMet (Sigma).
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bioRxiv - Cell Biology 2022Quote: ... mice received a single intraperitoneal dose (20 mg/kg) of LPS (Escherichia coli 0111:B4, L2630, Sigma). Mouse bone marrow monocyte derived macrophage (MDMs ...
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bioRxiv - Physiology 2022Quote: ... An additional control group received 2.5 mg/kg BW LPS from Escherichia coli (Sigma, L2630; Deisenhofen, Germany) (n=10 ...
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bioRxiv - Biophysics 2022Quote: ... The ligated pTrcHisB/PRNP construct was used to transform the Escherichia coli host strain BL21(DE3) (Novagen), genotype F′ ompT hsdSB (rB-mB- ...
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bioRxiv - Microbiology 2024Quote: All Escherichia coli strains used in this thesis were grown aerobically in Luria-Bertani (LB) broth (Sigma). E ...
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bioRxiv - Microbiology 2024Quote: ... coli culture at an optical density at 600 nm (OD600) = 0.84 1:2,000 in isotone (Sigma Aldrich) and measuring 75 µL thereof at a Coulter counter (Multisizer 4e (Beckman Coulter)) ...
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bioRxiv - Molecular Biology 2023Quote: ... mice were injected with 2mg/kg body weight phenol-extracted LPS (Escherichia coli O26:B6, Sigma-Aldrich) dissolved in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Biochemistry 2023Quote: ... Both plasmids (ATAD2/B) were transformed into Escherichia coli BL21(DE3) pLysS competent cells (Novagen, MA, USA) for protein expression.
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Neuroscience 2023Quote: ... adult C57Bl/6 mice were treated intra-peritoneally (IP) with LPS (Escherichia coli O55:B5, Sigma, L2880) at 5 mg/kg and brains were collected 3h (n=5 ...
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bioRxiv - Microbiology 2023Quote: ... coli strains was performed at 37 °C in LB (20g/L LB Broth (Lennox) (Sigma-Aldrich; L3022), 2xYT (tryptone 16g/L (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... coli and synthesized by Geneart (http://www.geneart.com).The genes were then sub-cloned into pET28a vector (Novagen) using the restriction sites NdeI and XhoI with an N-terminal His-tag in frame.
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bioRxiv - Bioengineering 2023Quote: ... coli expression and driven by a T7 promoter and lac operator in a pET28a expression vector (Novagen). Specifically ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli clones were grown at 37°C under aeration in liquid media Lysogeny Broth (LB) from SIGMA — or lactose supplemented McConkey agar plates and LB agar plates ...
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bioRxiv - Microbiology 2023Quote: ... coli RIL strains carrying the appropriate plasmid were grown in 1 l Luria-broth (LB) (Merck Millipore) supplemented with kanamycin (100 μg/ml ...
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bioRxiv - Microbiology 2024Quote: ... coli ST18 was grown in LB medium supplemented with 50 μg/ml 5-aminolevulinic acid (Sigma-Aldrich) at 37 °C to an OD600 of 0.3 ...
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bioRxiv - Immunology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Immunology 2020Quote: ADCP assays were performed using ExpiCHO-S target cells transiently transfected with SARS-CoV-2 S and fluorescently labeled with PKH67 Fluorescent Cell Linker Kits (Sigma Aldrich, Cat. Nr.: MINI67) as targets ...
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bioRxiv - Microbiology 2020Quote: ... rabbit polycolonal anti-SARS-CoV-2 S2 antibodies (1:1000) (Sinobiological Inc, Beijing, China) and anti-FLAG M2 antibody (1:1000) (Sigma, St. Louis, MO, USA), respectively.
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bioRxiv - Microbiology 2022Quote: ... and −7173 of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2023Quote: ... and -71)60 of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2022Quote: ... were resuspended in 100 μL of FC buffer containing primary antibodies (mouse anti-Spike S2, Thermo Fisher, Cat #: MA5-35946, 1:500 dilution) (mouse anti-Flag M2, Sigma, Cat #: F1804, 1: 500 dilution), or anti-SARS-CoV-2 human sera (1:100 dilution ...
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bioRxiv - Developmental Biology 2021Quote: ... 250–1000 embryos were collected and transferred into a Petridish containing Pronase E protease solution (Sigma-Aldrich, P5147-1G). When 20–30% embryos were hatched ...
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bioRxiv - Neuroscience 2021Quote: Cre-mediated recombination was induced via intraperitoneal injection of 4-hydroxytamoxifen (50:50 E and Z isomers, Sigma-Aldrich) dissolved in sunflower seed oil ...
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bioRxiv - Molecular Biology 2021Quote: ... and 3 μl cold lysisg/μl cold lysisl U6.3>Lhx5/Sox1/Six3 gRNA f+e was microinjected together with the Fast Green solution (Sigma) at a 2:1 ratio to ease the detection of the injection site of the developing neural tube and eye respectively with the help of borosilicate glass capillaries and electroporated as previously reported86–88 using the Intracel TSS20 OVODYNE Electroporator ...
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bioRxiv - Genomics 2020Quote: ... Cells were released from G1 by washing with YPD containing auxin and 0.15 mg/mL pronase E (Sigma Aldrich) three times ...
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bioRxiv - Biochemistry 2020Quote: ... cells were incubated with 2 ml Williams E media containing the indicated concentrations of oleic acid (Sigma 05508-5ML), 1 μg/ml Brefeldin A (Sigma B6542-5MG ...
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bioRxiv - Neuroscience 2021Quote: ... 2-(2-Furanyl)-7-(2-phenylethyl)-7H-pyrazolo[4,3-e][1,2,4]triazolo[1,5-c]pyrimidin-5-amine (SCH 58261, 50nM) were purchased from Sigma (St. Louis, MO). CGS 21680 and SCH 58261 were made up into a 5 mM stock solution in dimethylsulfoxide (DMSO) ...
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bioRxiv - Genomics 2022Quote: ... in TC-treated 6-well plates coated with 0.05 mg/mL Poly-D-Lysine (Sigma Aldrich A-003-E).
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bioRxiv - Biochemistry 2022Quote: ... 0.25 mg/ml Pepstatin A and 0.25 mg/ml E-64) or Roche cOmpleteTM EDTA-free protease inhibitor cocktail tablet (Millipore-Sigma). NP-40 (final concentration of 0.5% ...
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bioRxiv - Biophysics 2022Quote: ... E-cadherin knockdown was induced in shE-cadherin cells by adding 200 μM isopropyl-β-D-thiogalactoside (IPTG; Sigma) 72 h prior to experiments.
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bioRxiv - Genetics 2022Quote: ... cells were washed three times in YPD (30°C) containing 0.1 mg/ml Pronase E (Sigma-Aldrich; Catalogue # P5147), resuspended in SC minimal medium containing nocodazole (15μg/ml) ...
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bioRxiv - Immunology 2022Quote: ... DENV infection was then measured by intracellular detection of the E protein using the murine monoclonal 4G2 antibody (Millipore) followed by rabbit anti-mouse IgG-coupled to AF647 (Molecular probes) ...
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bioRxiv - Cancer Biology 2022Quote: Sirt1 conditional knockout E-NOTCH1-induced T-ALL-bearing mice were treated with vehicle only (corn oil; Sigma, C8267) or tamoxifen (Sigma ...
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bioRxiv - Bioengineering 2019Quote: ... Ice-cold acetone-fixed cryostat sections (10μm) were prepared and stained with hematoxylin and eosin(H&E) (Sigma-Aldrich) using routine methods ...
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bioRxiv - Genetics 2020Quote: ... Cell lysates were supplemented with IPH50 buffer and protease inhibitor cocktail (AEBSF, 1,10-Phenanthroline, Pepstatin A, E-64) (Sigma), centrifuged at 15,000rpm for 20 minutes ...
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bioRxiv - Plant Biology 2021Quote: Around 750 entire-4 (e-4) seeds were treated with the mutagenic substance Ethyl-Methane Sulfonate (EMS, Sigma m0880) at a concentration of 0.6% for 10 hours ...
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bioRxiv - Neuroscience 2021Quote: ... treated with poly-d-lysine(Sigma-Aldrich A-003-E, 1-2 hr at 37°C, 2 μL/well) followed by laminin (Thermo Fisher 23017015 ...
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bioRxiv - Biophysics 2021Quote: The ATPase activity of MutL or MutL(R-E) was measured by an ATPase/GTPase Activity Assay Kit (Sigma). The analysis was carried out with 5 μM protein in a 40 μL reaction mixture comprised of 20 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Microbiology 2022Quote: ... difficile spores were incubated with 10 µg mL−1 of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) with PBS for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were collected by filtration and promptly washed twice on the filter using one culture volume of H2O and then resuspended into YPD medium containing 0.2 mg/mL pronase E (Sigma).
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bioRxiv - Bioengineering 2022Quote: ... samples were stained with hematoxylin and eosin (H&E) through a 5- minute incubation in Harris Hematoxylin (Sigma Aldrich), followed by a wash in tap water and three dips in acid ethanol to destain ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... These sections were stained with Hematoxylin & Eosin (H&E) and periodic acid-Schiff (PAS) according to the manufacturer’s protocol (Sigma). The muscle sections were analyzed blindly by a pathologist ...
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bioRxiv - Microbiology 2024Quote: ... [cyano-(3-phenoxyphenyl)methyl] 3-(2,2-dichloroethenyl)-2,2- dimethylcyclopropane-1-carboxylate (i. e. cypermethrin, pesticide analytical standard, CAS 52315-07-8, supplier: Sigma-Aldrich, purity ≥ 90.0% ...
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bioRxiv - Neuroscience 2023Quote: ... The final concentrations of drugs used in the study: 10 μM of E-64 (Sigma-Aldrich, Cat.: #E3132-1MG), 60 nM of t-NED19 (Bio-techne ...
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bioRxiv - Biochemistry 2023Quote: ... The beads were washed three times with buffer E and then mixed with 60 μg of 3xFLAG peptide (SIGMA) in 60 μl of buffer E for 1 h with shaking at 1400 rpm at 4°C ...