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Citations for Millipore Sigma :
3251 - 3300 of 4935 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Antimicrobial and antibiofilm activity of human recombinant H1 histones against bacterial infectionsbioRxiv - Microbiology 2024Quote: Histones H1.2 was cloned into pET21a and expressed in Escherichia coli BL21(DE3) (Merk Millipore) while H1.0 and its C-terminal domain (CTD ...
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bioRxiv - Immunology 2023Quote: ... Lipopolysaccharides (LPS) Escherichia coli O111:B4 origin (L2630) and Evans blue (E2129) procured from Sigma, USA ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were expressed in Escherichia coli strain BL21(DE3) or Rosetta 2 (Novagen, Darmstadt, Germany) and cells were lysed with a cell disruptor (Avestin ...
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bioRxiv - Cell Biology 2023Quote: Human αSN cDNA was transformed into Escherichia coli Strain BL21 (DE3) (Novagen, Madison, Wis., USA) using the pNIC28-Bsa4 plasmid ...
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bioRxiv - Microbiology 2023Quote: ... Escherichia coli cells were counter-selected using Thiosulfate citrate bile salts sucrose (TCBS, Sigma-Aldrich) agar ...
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LRBA balances antigen presentation and T-cell responses via autophagy by binding to PIK3R4 and FYCO1bioRxiv - Immunology 2024Quote: ... followed by stimulation with LPS from Escherichia coli 055: B5 (20 µg/ml, Sigma-Aldrich) for 3 days in complete RPMI medium (L-glutamine ...
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bioRxiv - Microbiology 2024Quote: ... The BMDMs were primed with 100 ng/mL O26:B6 Escherichia coli LPS (Sigma®) and incubated overnight at 37°C with 5% CO2.
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bioRxiv - Immunology 2024Quote: LPS from Escherichia coli O111:B4 was obtained from Sigma-Aldrich (St. Louis, MO, USA). Dexamethasone (Dx ...
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bioRxiv - Neuroscience 2024Quote: ... lipopolysaccharides (LPS) from Escherichia coli O26:B6 (Sigma-Aldrich; 10, 100, and 1000 μg/mL), and recombinant TNF-α (Peptrotech;10 ...
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bioRxiv - Immunology 2024Quote: ... the cells were treated with 10ng/ml LPS (Lipopolysaccharides from Escherichia coli O55:B5; Sigma). After 3 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... were expressed in Escherichia coli BL21 (DE3) slyD- cells [56] carrying the plasmid pRARE2 (Novagen). Recombinant GST (vector pGEX-4T2 (Amersham) ...
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bioRxiv - Genetics 2021Quote: ... and 45 mg/kg body weight of Evans blue dye (Sigma-Aldrich, St. Louis, MO; E-2129) and saline was injected via the femoral vein ...
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bioRxiv - Developmental Biology 2021Quote: ... plates or coverslips were coated with 100 μg/ml poly-D-lysine (Merck Millipore, A-003-E) at 37°C overnight ...
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bioRxiv - Genomics 2020Quote: ... Cells were released from G1 by washing with YPD with 0.15 mg/mL pronase E (Sigma Aldrich) three times ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... bovine serum albumin lipid mix (palmitic acid 25 µmol/l; arachidonic acid 7 µmol/l; linoleic acid 15µmol/l; vitamin E 0.4µg/ml; all from Sigma), insulin (0.1 µmol/l ...
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bioRxiv - Microbiology 2021Quote: ... Lung paraffin sections were stained with Hematoxylin and Eosin (H&E) or Gomori’s Methenamine silver (GMS) (Sigma) for pathological examination ...
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bioRxiv - Immunology 2022Quote: ... and longitudinal sections were stained with H&E and Oil Red O (Millipore Sigma; Cat No O0625) to visualize BM adiposity ...
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bioRxiv - Neuroscience 2022Quote: ... Skin sections were processed for morphometric analysis using hematoxylin and eosin (H&E) (Sigma-Aldrich, #MHS32-1L) and immunofluorescence staining (e.g. ...
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bioRxiv - Immunology 2022Quote: ... paraffin embedded and sections (5 µm) prepared and stained with hematoxylin and eosin (H&E; Sigma-Aldrich). For clinical scoring 4-6 random field of views at 10x magnification from three sections 500 µm apart were assessed per colon ...
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bioRxiv - Cell Biology 2022Quote: ... 4 μm sections of paraffin-embedded samples were stained with hematoxylin and eosin (H&E, Sigma-Aldrich), periodic acid-Schiff (PAS ...
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bioRxiv - Biochemistry 2021Quote: ... Detection of HRP conjugates was carried out with 3,3′,5,5′-tetramethybenzidine (TMB/E, Millipore, Billerica, MA, USA). Detection of AP conjugated antibodies was carried out with SIGMAFAST p-nitrophenyl phosphate tablets (Sigma-N2770).
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bioRxiv - Cell Biology 2021Quote: ... The separated proteins were then transferred to the PVDF membranes (0.45 µm Immobilon®-E; Merck Millipore). Membranes were blocked either with 2.5% nonfat milk in TBST (50 mM Tris ...
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bioRxiv - Immunology 2020Quote: E-liquid mixture of 70% propylene glycol and 30% glycerol and 6mg/mL nicotine (purchased from Sigma) without flavors or additives were used ...
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bioRxiv - Microbiology 2020Quote: ... dithiothreitol (DTT) and trans-epoxysuccinyl-l-leucylamido(4-guanidino)butane (E-64) were purchased from Sigma Aldrich Chemical Co ...
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bioRxiv - Cancer Biology 2022Quote: ... M (Cat No: 1115-70-4) and E (Cat No: 989-51-5) were obtained from Sigma Chemical Co (St ...
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bioRxiv - Biophysics 2022Quote: ... For E-Cadherin stainings a 1:1000 dilution of Alexa 647-conjugated anti-rat (Sigma-Aldrich SAB4600186) was used ...
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bioRxiv - Cell Biology 2023Quote: ... Histological sections (5 µm) were subsequently stained with H&E or cresyl violet acetate (Sigma-Aldrich, #C5042). Immunohistochemistry to detect activated (cleaved ...
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bioRxiv - Bioengineering 2023Quote: ... MIK665 treatments were delivered in 2% vitamin E/d-α-tocopheryl polyethylene glycol 1000 succinate (Sigma-Aldrich) in NaCl 0.9% (wt/vol ...
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bioRxiv - Biophysics 2023Quote: ... purified using a 65% Percoll (Cytiva) cushion and then treated with 10 µM E-64 (Sigma, E3132) for 4-8 hours until mature merozoites had formed ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-SARS-CoV-2 proteins or lacking the fusion protein (∆Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland) and HTCC-77 (100 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Immunology 2021Quote: ... or 900-fold (SARS-CoV-1) diluted plasma samples before addition of THP-1 cells (20,000 cells per well; Millipore Sigma, Burlington, MA). After 19 h incubation at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed and then sequentially incubated with anti-SARS-CoV-2 CR3022 mAb [24] (1 μg/mL) and a HRP-conjugated goat anti-human IgG (Sigma, Cat#A6029) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Immunology 2023Quote: ... The first kit used was the Milliplex® SARS-CoV-2 antigen panel 1 IgA assay (Millipore Sigma, Cat No. HC19SERA1-85K) with the Wuhan-1 strain RBD and N proteins included ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with protease and phosphatase inhibitors (Inhibitor Cocktail cat. n. P8340, Sigma-Aldrich). Protein concentration was quantified with the Bradford assay (cat n ...
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bioRxiv - Cell Biology 2020Quote: ... free thiols were blocked by addition of 10 mM N-ethyl maleimide (Sigma) and incubated on a rotator at 4°C for 1 hour.
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bioRxiv - Microbiology 2019Quote: ... inner-membrane proteins were solubilized using 0.5% (w/v) N-lauryl sarcosine (Sigma) in a buffer containing 20 mM Tris-HCl [pH 7.5] and 150 Mm NaCl for 1.5 h at 4 °C on a roller ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% DMSO and 300 μM 3-maleimidobenzoic acid N-hydroxysuccinimide ester (MBS; Sigma) dissolved in microtubule stabilization buffer specific for algae (MTB ...
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Fgf4 is critical for maintaining Hes7 levels and Notch oscillations in the somite segmentation clockbioRxiv - Developmental Biology 2020Quote: ... then 10.5 g of 40% v/v solution of N-Methylacetamide (M26305, Sigma) in 1X PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were fixed sequentially with 2mM di(N-succinimidyl) glutarate (Sigma-Aldrich, #80424) and 1% formaldehyde (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... A standard mounting medium was prepared with 1.25% n-propyl gallate (Sigma, P3130), 75% glycerol (bidistilled ...
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bioRxiv - Biochemistry 2022Quote: ... Iss10 constructs were cloned as N-terminal Strep-tag fusions into pACYCDuet (Novagen). Combinations of Red1 and Iss10 constructs were co-expressed in E ...
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bioRxiv - Cell Biology 2022Quote: ... Negative controls were prepared by adding 10 mM N-ethymaleimide (NEM; Sigma-Aldrich) to cell lysates ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... o/n at 4 °C + 2 hr at 37°C] (Sigma; C3865, USA), CD73 (1:100 in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... in acetic acid (0.02 N) or human plasma fibronectin (5 µg/mL; Millipore) in PBS overnight at 4°C and rinsed with imaging medium to remove the coating solution ...
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bioRxiv - Evolutionary Biology 2019Quote: ... a lysation buffer containing 0.5 % (w/v) N-Lauroylsarcosine (Sigma Aldrich L9150-50G), 50 mM Tris-HCl (Thermo Fisher Scientific 15568025) ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... aerated water with 0.05% DASPEI (2-4-dimethylamino-N-ethylpyridinium iodide; Sigma Aldrich) to label superficial and canal neuromasts ...
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bioRxiv - Neuroscience 2019Quote: Clozapine-N-oxide (CNO) (C0832, Sigma-Aldrich, dissolved in saline, 1 mg/kg) or saline was injected i.p ...
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bioRxiv - Cell Biology 2021Quote: Ezrin constructs in pET-28a(+) (N-terminal hepta-His tag, Novagen, Madison, WI): hEzrin wildtype (His-Ezrin/WT ...
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bioRxiv - Biochemistry 2021Quote: ... For carrying out SHAPE with 2 mM N-Methylisatoic anhydride (NMIA, Millipore-Sigma) with or without U1 snRNP ...