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Citations for Millipore Sigma :
3201 - 3250 of 4935 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Nuclei were stained using Hoechst (2 μM, Sigma, cat. n. 14530, US). The gel volume was included for the calculation of antibody dilution ...
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bioRxiv - Immunology 2023Quote: N-Formyl-Met-Leu-Phe [FMLP] (Sigma-Aldrich; Saint Louis, MO, USA) was added to the top of the Matrigel to induce a chemokine gradient ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... and loaded onto the Amersham Hybond-N+ membrane (Millipore, Boston, MA, USA). The membranes were then subjected to UV cross-linking (10 minutes) ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR was performed using the REDExtract-N-Amp PCR Readymix (Sigma-Aldrich). The following primers were used for genotyping ...
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bioRxiv - Developmental Biology 2023Quote: ... N-[2-(p-Bromocinnamylamino)ethyl]-5- isoquinolinesulfonamine dihydrochloride (H89, B1427, Sigma-Aldrich), were used in this study.
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bioRxiv - Bioengineering 2023Quote: ... PEG was conjugated to nanoparticles using 7 mM N-Hydroxysulfosuccinimide (NHS) (Sigma) and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM 1,4-Dithiothreitol (DTT) and 5 mM N-Ethylmaleimide (Sigma-Aldrich)) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... N-diisopropyl ethyl amine were purchased from Sigma Aldrich (St. Louis, MO). HPLC grade acetonitrile was purchased from Supelco (Bellefonte ...
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bioRxiv - Molecular Biology 2023Quote: ... SK-N-BE cells were dissociated with Trypsin 1X (Sigma-Aldrich, #T4549) and 20,000-30,000 cells/well were plated on glass coverslips in 24-well plates.
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bioRxiv - Cell Biology 2023Quote: ... 75μl of 3mM 4-nitrophenyl N-acetyl-β-d-glucosaminide (Sigma #N9376) for in 0.1M citrate buffer (0.1M sodium citrate ...
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bioRxiv - Immunology 2023Quote: ... Cells treated with antioxidants were cultured with 10 mM N-acetylcysteine (Sigma). For measuring T cell effector ...
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bioRxiv - Cell Biology 2023Quote: ... N-(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride (A3281, Sigma, MO, USA); 500nM ZSTK474 (S1072 ...
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bioRxiv - Biochemistry 2023Quote: ... and N-hydroxysulfosuccinimide (Sulfo-NHS) were from Sigma-Aldrich (St. Louis, MO). Lauryl maltose neopentyl glycol (LMNG ...
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bioRxiv - Cell Biology 2024Quote: ... or with 15 mM n-acetylcysteine (NAC) for 2 hours (Sigma, A7250) prior to co-treatment with CSE ...
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bioRxiv - Cell Biology 2024Quote: ... 100 mM EDTA disodium (Biotechnik GmbH) and 1% sodium N-Laurylsarcosin (Sigma,) in distilled water ...
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bioRxiv - Immunology 2024Quote: ... then the reaction was stopped by 0.5 N sulfuric acid (Sigma Aldrich). The absorbance of wells was immediately measured at 450 nm with a microplate reader (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... 1 mM N-acetyl-L-cysteine (Sigma-Aldrich, St Louis, MO, USA), 2.0% Noggin/R-spondin-conditioned media ...
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bioRxiv - Immunology 2024Quote: ... rabbit anti-Fractin (N-terminal fragment of actin) (1:1000, Millipore, AB3150) Abs were incubated with agitation in PBT-BSA for 1 hr at RT and 72 hrs at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... tissue was dounced in Nuclei EZ Lysis Buffer (Sigma-Aldrich N 3408), incubated on ice for five minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 µM LLnL (N- Acetyl-Leu-Leu-Norleu-aldehyde) (Sigma-Aldrich). Reactions were incubated at 30 °C for 2 h and terminated adding SDS/PAGE loading buffer ...
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bioRxiv - Synthetic Biology 2021Quote: ... The membranes were blocked and incubated with specific antibodies: HA-tagged spike protein was detected with anti-HA antibody (H9658, Sigma-Aldrich, 1:10,000), hACE2 was detected with anti-ACE2 antibody (sc-390851 ...
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bioRxiv - Genomics 2023Quote: ... used for spike-in controls for ChIPs of TWIST1 depletions were cultured in Complete ES Cell Medium with 15% FBS (Millipore, ES-101-B), 25 ng/ml bFGF ...
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bioRxiv - Cell Biology 2020Quote: ... coli) and cloned into the HindIII and EcoRI sites of pTAC-MAT-Tag2 (Sigma-Aldrich) to yield the pTac-Cat plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... LPS (lipopolysaccharides from Escherichia coli O55:B5) was from Sigma-Aldrich (St. Louis, MO, USA).
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bioRxiv - Molecular Biology 2020Quote: ... coli and the cDNAs were synthesized by IDT and were cloned into pCDF duet (Novagen) in frame with an N terminal 6 x HIS tag for bacterial expression ...
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bioRxiv - Immunology 2019Quote: ... containing LPS from Escherichia coli O55:B5 at 40 mg/ml (Sigma Chemical Co, St) (Louis ...
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bioRxiv - Bioengineering 2020Quote: ... coli cultures were grown at 37 °C in LB (both broth and solid) (Sigma, US). Appropriate antibiotics were supplemented with the following working concentrations ...
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bioRxiv - Neuroscience 2020Quote: ... coli 0111:B4 lipopolysaccharide (LPS) (Sigma-Aldrich, cat#L3012, Ec#297-473-0, lot#WI150506) was dissolved in saline and used at a dose of 0.1 mg/kg ...
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bioRxiv - Microbiology 2020Quote: ... Escherichia coli strains were grown in liquid or solid Luria Bertani (LB) medium (Sigma Aldrich) at 37°C (57) ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli in Lennox’s Lysogeny Broth (LB-Lennox) medium (10 g L−1 peptone (EMD Millipore), 5 g L−1 yeast extract (EMD Millipore) ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli DH5α strains were routinely cultivated in lysogeny broth (LB) medium (LB Broth, Sigma Aldrich), 37°C ...
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bioRxiv - Genomics 2020Quote: ... anaesthetised snails were injected as above with 0.5 mg/mL lyophilised Escherichia coli (Sigma-Aldrich, Steinheim ...
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bioRxiv - Neuroscience 2020Quote: ... Antibodies against adenomatous polyposis coli (1:250; APC or CC1, Merck Millipore, Billerica, MA, USA) or GFP (1:8000 ...
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bioRxiv - Microbiology 2021Quote: ... coli O111:B4 (Catalog# L2630-10MG, lot 114M4009V) were purchased from Sigma (St. Louis, MO). Budesonide (Catalog# S1286) ...
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bioRxiv - Molecular Biology 2021Quote: ... coli wild type (wt) Hfq protein and its mutants were expressed from pET15b vector (Novagen) and purified as described [38] ...
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bioRxiv - Cell Biology 2021Quote: ... coli DH10B cells and cells harboring recombinant plasmids selected on Luria Bertani (LB; Sigma-Aldrich) agar plates with 50 μg/ml kanamycin ...
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bioRxiv - Molecular Biology 2022Quote: ... coli HMS174 (DE3) (F- recA1 hsdR(rK12- mK12+) Rif R (DE3)) was purchased from Novagen. E ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... as previously performed,24 γ-irradiated LPS from Escherichia coli O111:B4 (BioXtra; Sigma-Aldrich) dissolved in PBS was IV-injected at the dose of 2.4 ng ...
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bioRxiv - Microbiology 2021Quote: ... coli membrane fractions were determined using a bicinchoninic acid (65) protein assay kit (Sigma-Aldrich) with BSA (Interchim ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells by the heat-shock approach as described in the supplier’s manual (Novagen/Agilent) and by antibiotic selection using Zeocin (Invitrogen/ThermoFisher) ...
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bioRxiv - Biochemistry 2021Quote: RIG-I was expressed using pET28 SUMO vector in Escherichia coli strain Rosetta (DE3) (Novagen). Soluble cell lysate was purified using a Ni2+-nitrilotriacetate (QIAGEN ...
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bioRxiv - Immunology 2020Quote: ... To stimulate cells 0.3 ng* μl−1 LPS (Lipopolysaccharide from Escherichia coli 0111:B4, Sigma) was added and cells were incubated for 4 hours at 37°C and 7 % CO2 ...
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bioRxiv - Microbiology 2021Quote: ... The BMDMs were primed with 100 ng/mL O26:B6 Escherichia coli LPS (Sigma®) and incubated overnight at 37°C with 5% CO2.
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bioRxiv - Biophysics 2019Quote: ... GFP-PRC1 isoform 2 proteins were expressed via BL21(DE3) Rosetta Escherichia coli cells (Novagen). Full length PRC1 was expressed for 4 hours at 18 °C after induction with 0.5mM IPTG ...
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bioRxiv - Biochemistry 2022Quote: ... The cloned vector was transformed into Escherichia coli BL21 HMS174(DE3) chemically competent cells (Novagen). A colony was picked and placed into an overnight outgrowth ...
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bioRxiv - Immunology 2022Quote: ... coli and protein was isolated and purified on His-binding nickel resin (Novagen, Madison, Wisconsin). Nitrophenyl-haptenated ovalbumin (NP-OVA ...
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bioRxiv - Neuroscience 2022Quote: ... anti-adenomatous polyposis coli clone CC1 (APC/CC-1) (1:100, mouse, Millipore Cat# OP80) anti-platelet derived growth factor receptor alpha (PDGFRa ...
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bioRxiv - Evolutionary Biology 2024Quote: ... coli C wildtype and phage resistant strains were diluted 10 times in Ringer’s solution (Sigma). 50 µL of each 10 times diluted overnight culture (5 x 106 cells ...
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bioRxiv - Neuroscience 2024Quote: ... we used activated microglia induced by lipopolysaccharides (LPS, Cat# Escherichia coli O111:B4, Sigma-Aldrich) in this study ...
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Antimicrobial and antibiofilm activity of human recombinant H1 histones against bacterial infectionsbioRxiv - Microbiology 2024Quote: Histones H1.2 was cloned into pET21a and expressed in Escherichia coli BL21(DE3) (Merk Millipore) while H1.0 and its C-terminal domain (CTD ...