Labshake search
Citations for Millipore Sigma :
3101 - 3150 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... The PCR product designed for homologous recombination was transformed using lithium acetate (Sigma) as previously described (Tripp et al. ...
-
bioRxiv - Cell Biology 2021Quote: ... as well as by quantitative PCR (KAPA Biosystems, Sigma-aldrich, St. Louis MA). The sequencing libraries were clustered on a single lane of a flowcell ...
-
bioRxiv - Biochemistry 2021Quote: ... DNA fragments were PCR-amplified with KOD Hot Start Master Mix (Novagen®) from genomic DNA of M ...
-
bioRxiv - Synthetic Biology 2021Quote: ... DNA fragments were either PCR-amplified with oligonucleotide primers (Sigma-Aldrich and IDT) or ordered as gBlocks from IDT and cloned into Gateway pENTR plasmids ...
-
bioRxiv - Microbiology 2020Quote: ... Inverse PCR using this construct was carried out with KOD polymerase (EMD Millipore) according to manufacturers instructions ...
-
bioRxiv - Microbiology 2021Quote: PCR reactions were carried out with either KOD Hot Start DNA Polymerase (Sigma) or iProof DNA Polymerase (Bio-Rad) ...
-
bioRxiv - Cell Biology 2020Quote: ... Conventional PCR was performed using KOD Xtreme Hot Start DNA Polymerase (EMD Millipore). The fragments were cloned into pCR-Blunt II TOPO using the Zero Blunt TOPO PCR Cloning Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2022Quote: ... Quantitate PCR was conducted using KAPA SYBR FAST Master Mix (Sigma-Aldrich, KK4611) on a Roche Lightcycler 96 (Roche Life Science) ...
-
bioRxiv - Microbiology 2022Quote: ... The RNA was subsequently checked for DNA by PCR using RedTaq readymix (Sigma). RNA integrity was assessed using 2100 Bioanalyzer (Agilent ...
-
bioRxiv - Neuroscience 2020Quote: ... Nectin-3 alpha was PCR amplified (KOD hot start DNA polymerase, Sigma-Aldrich) from a mouse brain cDNA library using the forward primer ...
-
bioRxiv - Genetics 2019Quote: ... PCR plates were filled with a master mix for KOD Hotstart (Novagen #71086) PCR reactions containing Lister427 gDNA according to manufacturer’s specifications and primer pairs specific to each ORF were transferred into the associated well number for each plate by TECAN Freedom EVO 150 pipetting instrument ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... PCR product was digested with NcoI and XhoI and cloned into pET28a (Novagen) in frame with a C-terminal his-tag coding sequence E ...
-
bioRxiv - Cancer Biology 2019Quote: ... with PCR primers with overhangs containing restriction sites for SpeI and NotI (Sigma). pLVX-EF1α-IRES-ZsGreen1 (Cat# 631982 ...
-
Orange is the new white: taxonomic revision of Antarctic Tritonia species (Gastropoda: Nudibranchia)bioRxiv - Zoology 2020Quote: ... 3.3 μL REDExtract-N-Amp PCR ReadyMix (Sigma Aldrich, St. Louis, MO, USA), 0.3 μL of each primer ...
-
bioRxiv - Molecular Biology 2021Quote: ... bL33 were PCR-amplified and cloned individually into the pET24(a) plasmid (Novagen). Similarly ...
-
bioRxiv - Genomics 2019Quote: ... The libraries were PCR amplified with KAPA HiFi Hotstart Ready mix (Sigma-Aldrich) and barcoded Nextera custom primers 17 and finally size-selected (250–350 bp ...
-
bioRxiv - Biochemistry 2021Quote: ... The purified PCR products were concentrated using Amicon Ultra centrifugal filter units (Millipore) and buffer exchanged against 10 mM HEPES pH 7.0 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were visualized after agarose gel electrophoresis using GelRed® stain (Sigma) and a GelDoc imaging system (Bio-Rad).
-
bioRxiv - Biochemistry 2020Quote: All PCR reactions were carried out using KOD Hot Start DNA polymerase (Novagen). All RT-PCRs were carried out using Takara’s PrimeScript High Fidelity RT-PCR kit (R022A).
-
bioRxiv - Genetics 2020Quote: ... Cell lines were tested for mycoplasma contamination using the Mycoplasma PCR ELISA (Sigma).
-
bioRxiv - Bioengineering 2022Quote: ... After digestion the PCR product was ligated into the pET30a vector (EMD Millipore). The resulting construct encodes EcSTH with both an N-terminal Hisx6-tag and a C-terminal FLAG-tag.
-
TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thalianabioRxiv - Plant Biology 2022Quote: ... It was generated by PCR amplification (DNA KOD Hot-start DNA Polymerase, Novagen) and cloned into the pENTRD-TOPO Gateway vector using the manufacturer’s protocol and confirmed by sequencing ...
-
bioRxiv - Neuroscience 2024Quote: ... Primers for SYBR Green-based PCR of mRNA were obtained from Sigma Aldrich, miRCURY LNA assays for qPCR of miRNA were purchased from Qiagen ...
-
bioRxiv - Biochemistry 2024Quote: ... PCR reactions were performed with KOD Hot Start DNA Polymerase (Sigma 71086-3) using a Mastercycler X50a (Eppendorf) ...
-
bioRxiv - Biophysics 2024Quote: ... sample wells (Fig. S14) were made from half-cut PCR tubes (Sigma-Aldrich) glued to the prepared slides with epoxy (Ellsworth Adhesives) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... PCR reactions were performed using a high-fidelity polymerase (KOD-Xtreme, Merck Millipore) according to manufacturer protocol ...
-
bioRxiv - Microbiology 2023Quote: ... PCR assay was conducted by using gene specific primers procured from Sigma-Aldrich. The gene PA3617 (recA ...
-
bioRxiv - Biochemistry 2023Quote: ... The PCR product was subcloned and inserted into a pET-24b (+) plasmid (Novagen) digested with NheI/XhoI placing the gene upstream of a sequence encoding six histidine residues ...
-
bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR was performed using FastStart Universal SYBR Green Master Mix (Sigma, 4913850001). The aggregates of three housekeeping genes (B2m ...
-
Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... qPCR reactions were prepared using SYBR green PCR Jumpstart Taq ReadyMix (Sigma-Aldrich), ROX Reference Dye (ThermoFisher Scientific ...
-
Extrusion fountains are hallmarks of chromosome organization emerging upon zygotic genome activationbioRxiv - Molecular Biology 2023Quote: ... Sanger sequencing of the PCR products was performed using a commercial service (Sigma). The sequencing traces were examined and the fish carrying A to G mutation were selected ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... PCR amplification was done by using Taq DNA polymerase (Sigma-Aldrich®, USA), and the PCR product was digested with the restriction enzymes shown in Table 1 ...
-
bioRxiv - Microbiology 2024Quote: ... The reaction mix was: 0.45 µL DMSO for PCR (Sigma; cat# D8418-50mL), 0.0045 µL SYBR Green I (Sigma ...
-
bioRxiv - Microbiology 2024Quote: ... PCR assay was conducted by using gene specific primers procured from Sigma-Aldrich. The gene rpsU was kept as an endogenous control ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... We carried out polymerase chain reactions (PCRs) with Hot Start polymerase (MERCK MILLIPORE) and performed colony PCRs with Taq polymerase (NEB) ...
-
bioRxiv - Microbiology 2021Quote: ... Parasitemia was determined daily following tail bleeds and assayed by automated fluorescent cell counting with a Muse cytometer (Merck-Millipore, detection limit at 102 parasites/mL) according to the manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2023Quote: ... rabbit β-gal antibody (MP 559761, dilution 1/500), rabbit anti DsRed (clontech 632496, dilution1:200) for tdTomato detection and rabbit polyclonal anti-Laminin (Sigma-Aldrich, Cat# L9393, dilution 1:1000). Following washes in PBS-T (0.05% Tween20 in PBS) ...
-
bioRxiv - Microbiology 2023Quote: ... Parasitemia was determined daily following tail bleeds and assayed by automated fluorescent cell counting with a Muse cytometer (Merck-Millipore, detection limit at 102 parasites/mL) according to the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2020Quote: ... and b (chl b) were identified (RT and maxima absorption spectra) by direct injection of chl a and chl b standards (Sigma-Aldrich C-5753 and 00538, respectively) under the same HPLC conditions ...
-
bioRxiv - Neuroscience 2021Quote: ... Nitrocellulose membranes were incubated in Odyssey blocking solution from Licor (30 min at RT) and later blotted with primary mouse anti-Htt MAB5492 from Millipore (12–16 h at 4 °C). Membranes were washed 3 times in PBS-Tween 0.1% and then incubated with secondary Alexa680-conjugated goat anti-mouse (Licor ...
-
bioRxiv - Biochemistry 2019Quote: ... secondary antibody incubation was carried out for 1 h at RT followed by staining of the nuclei with DAPI (Sigma, Cat. No. 32670, Lot No. BCBD9022V) and mounting of the sections with ProLong Gold Antifade Mountant (Invitrogen ...
-
bioRxiv - Neuroscience 2022Quote: ... for 1 hour at RT and incubated with antibody against β-III-tubulin (mouse anti-β-III-tubulin isoform, 1:500; Millipore cat#MAB1637, RRID: AB_2210524) and antibody against GFAP (chicken anti-GFAP ...
-
bioRxiv - Microbiology 2024Quote: ... Some antibodies used were directly coupled to HRP and incubated 1 h at RT (FLAG-HRP, Sigma-Aldrich, A8592, 1/5000; Myc-HRP, Sigma-Aldrich, A5598, 1/5000; GAPDH-HRP, Sigma-Aldrich, G9295, 1/5000). Bioluminescence emission was subsequently measured using Clarity ECL Western Blotting Substrate (Bio-Rad ...
-
bioRxiv - Plant Biology 2020Quote: ... retention times (using the Kovats’ index (49) NIST database for polar columns) and by co-injection of authentic compound standards (Sigma).
-
bioRxiv - Microbiology 2020Quote: ... cells were washed 3 times with PBS before adding fresh post-infection media containing 1% microcrystalline cellulose (Avicel, Sigma Aldrich). Cells were further incubated at 37 °C for 24 h ...
-
mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... cells were washed 3 times with PBS and permeabilized at room temperature for 15 min with 0.1% Triton X-100 (Sigma Aldrich). Next ...
-
bioRxiv - Biophysics 2020Quote: ... cells were washed three times with 2 mL phosphate-free HEPES-buffered Hanks’ balanced salt solution (HHBSS) containing 20 mM HEPES (Sigma), pH 7.4 and resuspended in 1.5 mL of the same buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... Chromatin was diluted 10 times and incubated overnight at 4 °C with 20 µL Protein A/G MagnaChIP beads (Millipore) and 8 µg anti-H3K27me3 (Millipore ...
-
bioRxiv - Cell Biology 2020Quote: ... Embryos were collected at appropriate time points from 5-8 week old hormone-primed (5 IU each, pregnant mare serum gonadotropin (Sigma) and human chorionic gonadotropin (Sigma) ...
-
bioRxiv - Cell Biology 2020Quote: ... For copper supplement experiments cells were incubated for the indicated time at 37°C in medium containing CuCl2 (Sigma-Aldrich).