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Citations for Millipore Sigma :
3051 - 3100 of 4935 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... coli were pelleted and re-suspended with various concentrations of sodium sulfite (Millipore Sigma) in water ...
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bioRxiv - Evolutionary Biology 2022Quote: ... coli MG1655 using Luria-Bertani (LB) or Mueller Hinton Broth (MHB) medium (Sigma-Aldrich). All control and constructed plasmids are listed in Supplementary table.S3 ...
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bioRxiv - Neuroscience 2022Quote: LPS (1 mg/kg; from Escherichia coli, serotype 055:B5; Sigma-Aldrich, L-2880), diluted in a volume of 100 μl of 0.9% saline solution was administered intraperitoneally ...
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bioRxiv - Biochemistry 2024Quote: Synthetic genes codon-optimized for Escherichia coli were cloned into the pET15b vector (Novagen) with an N-terminal hexahistidine (His6)-tag ...
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bioRxiv - Neuroscience 2024Quote: ... Lipopolysaccharide (LPS) from Escherichia coli was purchased from Sigma Aldrich (O111:B4, Batch #019M4009V) (UK) ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 (DE3) genomic DNA and inserted into the pET-22b expression vector (Novagen) between the NcoI and XhoI cloning sites using an In-Fusion cloning kit (Clonetech) ...
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bioRxiv - Microbiology 2023Quote: ... coli XL-I Blue cells with the Sigma GenElute Plasmid Miniprep Kit (Sigma-Aldrich) as specified by the manufacturer.
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bioRxiv - Neuroscience 2024Quote: A single dose of lipopolysaccharide (LPS) from Escherichia coli O111:B4 (Sigma-Aldrich, #L2630) at the concentration of 5mg/kg dissolved in sterile saline (NaCl 0.9% ...
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bioRxiv - Biophysics 2023Quote: ... GST-tagged Elf1 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Glutathione Sepharose 4 Fast Flow resin (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... coli strain MG1655 on agar plates containing 0.3 mM diaminopimelic acid (DAP; Sigma-Aldrich). To induce expression from the PBAD promoter ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were stimulated with 10 ng/mL LPS (Escherichia coli O127:B8; Sigma Aldrich) for 15 min or 1 h to analyze the expression of IκBα ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... or dexamethasone (10 µM) and challenged with LPS (Escherichia coli O127:B8; Sigma Aldrich) (10 ng/mL ...
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bioRxiv - Genetics 2024Quote: ... coli was performed on Lennox LB containing kanamycin (25 µg/mL, Sigma Aldrich, K1377) or ampicillin (100 µg/mL ...
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bioRxiv - Immunology 2021Quote: ... pTT3-HKU1 or pTT3-OC43 Spike were transfected into suspension-adapted 293T cells using 293 Free transfection reagent (EMD Millipore Cat# 72181) or PEI transfection reagent (PolySciences Inc ...
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bioRxiv - Immunology 2021Quote: ... For RBD inoculations eight-week-old male BALB/c mice were immunized with 7.5 μg of the receptor binding domain of Spike protein (kindly gifted by Andrea Gamarnik, Argentina) in 75 μL Complete Freund’s Adjuvant (CFA, Sigma, St. Louis, MO) via subcutaneous injection and boosted 2 weeks later with 5 μg of RBD in 100 μL Incomplete Freund’s Adjuvant (IFA ...
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bioRxiv - Physiology 2019Quote: ... Secondary antibodies (see Table S1) were used at 1:500 dilution in 0.1% Tween-20/PBS with DAPI (Sigma-Aldrich, St. Louis, MO, USA). Samples were treated in the secondary antibody solution at 4°C overnight ...
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Microplastic consumption induces inflammatory signatures in the colon and prolongs a viral arthritisbioRxiv - Immunology 2021Quote: ... embedded in paraffin and sections stained with hematoxylin and eosin (H & E; Sigma-Aldrich, Darmstadt, Germany). For immunohistochemistry ...
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bioRxiv - Developmental Biology 2021Quote: H&E staining on paraffin sections was performed following the producer recommendations (Sigma, Procedure No. HHS).
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bioRxiv - Microbiology 2019Quote: ... sectioned with a microtome (5 µm) and stained with hematoxylin and eosin (H&E; Sigma, USA) for histological examination [23] ...
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bioRxiv - Cell Biology 2019Quote: ... These parasites were immediately resuspended in media plus 7.5mM GlcN and 20 µM E-64 (Sigma) and incubated at 37°C in a still incubator for 6hrs ...
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bioRxiv - Physiology 2021Quote: ... and plated in 6-well plates (0.25×106 cells per well) in William’s Medium E (Sigma) supplemented with antibiotics and 5% fetal bovine serum (FBS ...
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bioRxiv - Plant Biology 2020Quote: ... as a nitrogen source and supplemented with 0.1% sucrose and 1% agar (Type E, Sigma A4675), pH=5,8 ...
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bioRxiv - Developmental Biology 2020Quote: PLAT-E cells and (where stated) iPSCs were cultured in GMEM basal medium (GMEM (Sigma-Aldrich), 1xNEAA (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... DNA content was assessed using a commercial kit: DNA quantitation kit e Fluorescence assay (Sigma Aldrich) in a 96-well plate ...
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bioRxiv - Biochemistry 2021Quote: Proteinase K (E. Album) and ammonium sulfate were purchased from Sigma (Sigma-Aldrich, St. Louis, MO) and used without further purification ...
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bioRxiv - Cell Biology 2022Quote: ... The schizonts were resuspended in 40 ml fresh complete RPMI with 10 μM E-64 (Sigma), a cysteine protease inhibitor that prevents merozoite release from schizonts by inhibiting rupture ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T-ACE2-TMPRSS2 cells were pretreated with indicated concentrations of EST/E-64D (E64d) (Sigma, 330005) or camostat mesylate (Camostat ...
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bioRxiv - Microbiology 2022Quote: ... Vero-ACE2-TMPRSS2 cells were pretreated with indicated concentrations of EST/E-64D (E64d) (Sigma, 330005) or camostat mesylate (Camostat ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... Hematoxylin & Eosin (H&E) staining of pancreatic sections were performed according to the manufacturer’s protocol (Sigma). The islet area was quantified using the same using ImageJ software.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... For hematoxylin/eosin (H&E) staining slides were deparaffinized as above followed by hematoxylin (Sigma, GHS232) solution for 10 minutes followed tap water for 15 minutes and eosin (Merck ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... For the preparation of the e-liquids were used propylene glycol (PG) (Sigma Aldrich, P4347-500ML), vegetable glycerol (VG ...
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bioRxiv - Microbiology 2023Quote: ... blood was collected from healthy volunteers by venipuncture and mixed with K3EDTA (#E-0270, Sigma-Aldrich). The donors were two females and one male ...
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bioRxiv - Cell Biology 2023Quote: ... Degradation of the endogenous CENP-E is achieved through addition of 500 μM IAA (Sigma-Aldrich) (Owa & Dynlacht ...
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bioRxiv - Cell Biology 2023Quote: ... and the suspension was seeded into a Poly-D-lysine (PDL; Sigma-Aldrich, A-003-E)-pre-coated 75 cm2- cell culture flask containing 10 ml of the supplemented DMEM ...
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bioRxiv - Cancer Biology 2023Quote: ... the pellet was resuspended in William’s E Medium and layered onto Percoll® solution (Sigma-Aldrich) (10ml Percoll®+ 1.25ml PBS 10X + 1.25 ml H2O. ...
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bioRxiv - Microbiology 2023Quote: ... while the experimental group received oral administration of vitamin E (Sigma-Aldrich; Burlington, MA, USA; 258024) (500 mg/kg of body weight ...
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bioRxiv - Cell Biology 2024Quote: ... The primary antibodies were used as follows: rabbit anti-CENP-E (1:100, C7488; Sigma-Aldrich), rabbit anti-Spindly/CCD99 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... E and 8C) brains were fixed in 2% paraformaldehyde with 0.1% Triton X-100 (Sigma, T9284). for 20 minutes and then changed to a 4% paraformaldehyde ...
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bioRxiv - Immunology 2022Quote: PBMC or expanded T cell lines were stimulated for 5h at 37°C with or without SARS-CoV-2 peptides (2 μg/ml) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich). Cells were stained with the yellow LIVE/DEAD fixable dead cell stain kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: MERS-CoV and SARS-CoV-2 strains were obtained from different collaborators and passaged once in VeroE6 cells in DMEM (Sigma Aldrich) supplemented with 2% fetal bovine serum (Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: PBMC or Expanded T cell lines were stimulated for 5h at 37°C with or without SARS-CoV-1/2 peptide pools (2 μg/ml) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich). Cells were stained with the yellow LIVE/DEAD fixable dead cell stain kit (Invitrogen ...
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bioRxiv - Immunology 2020Quote: Expanded T cell lines were stimulated for 5h at 37°C with or without SARS-CoV-2 peptide pools (2 μg/ml) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich). Cells were stained with the yellow LIVE/DEAD fixable dead cell stain kit (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ADCP assay was performed using ExpiCHO cells stably expressing full-length wild-type SARS-CoV-2 S (target cells) and labelled with PKH67 (Sigma Aldrich) as targets ...
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bioRxiv - Immunology 2022Quote: ... 1 – 1.5 million cells were plated into 96-well V-bottom plates in 200mL R10 and stimulated with SARS-CoV-2 peptide pools (2ug/mL for each peptide) in the presence of Brefeldin A (Sigma-Aldrich) and monensin (GolgiStop ...
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bioRxiv - Immunology 2022Quote: ... The sections were then incubated overnight with primary antibodies directed against SARS Nucleocapsid protein (1/500; mouse monoclonal; clone 1C7C7; Sigma-Aldrich), ionised calcium-binding adapter molecule 1 (Iba1 ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transfected with pCG1-SARS-CoV-2_S plasmid using X-tremeGENE™ 9 DNA transfection reagent (Millipore-Sigma: 06365779001) (ratio 3:1 μl/μg DNA) ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transfected with pCG1-SARS-CoV-2_S plasmid using X-tremeGENE™ 9 DNA transfection reagent (Millipore-Sigma: 06365779001) (ratio 3:1 μl/μg DNA) ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 mM sodium orthovanadate) and 1% N-ocetylglucoside (NOG, Sigma-Aldrich) for 10 min on ice ...
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bioRxiv - Cell Biology 2020Quote: ... Antibodies are: Rabbit anti-H3(N-terminal) (Sigma, Catalogue#: H9289, 1:1000), H3K9me3 (Diagenode ...
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bioRxiv - Cell Biology 2020Quote: HEK293 cells were cultured in DMEM-F12 (cat n. D6421, Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...