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Citations for Millipore Sigma :
3101 - 3150 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: THP-1 monocytes were plated on coverslips at a seeding density of 30000 cells/well in a 24-well plate and differentiated with 20 ng/mL phorbol myristate acetate (Sigma). After 18 h ...
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bioRxiv - Immunology 2022Quote: ... Liquid inocula for the mice infections were prepared by inoculating Pa from the plates in LB broth (#L3522, Sigma-Aldrich) overnight at 37 °C and keeping in a rotating shaker at 220 rpm for 16 h (logarithmic phase of infection) ...
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bioRxiv - Synthetic Biology 2022Quote: ... the cells were scraped from plates and resuspended in 200 mL transformation buffer (per Liter: 50 g sucrose (Sigma S0389), 250 μL silwet-77 (Lehle Seeds VIS-30) ...
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bioRxiv - Plant Biology 2022Quote: ... thaliana seedlings for each line grown on a ½ MS agar plate using the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... EBs were then seeded onto growth factor reduced Matrigel-coated plates in E6N2 containing 1 μg/ml laminin (Sigma Aldrich). After another 7 days (Day 0) ...
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bioRxiv - Microbiology 2022Quote: MDCK-SIAT1 cells were infected with the PR8 NP:WT/F346S viruses at an MOI of 5 TCID50/cell in a 6 well plate in the presence of 100µg/mL of cycloheximide (Sigma-Aldrich). Infected cells were harvested at 2 and 6hrs post-infection ...
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bioRxiv - Microbiology 2022Quote: ... Plates were washed as before and 100μl/well rabbit anti-mouse IgG1 or IgG2a conjugated to HRP (Sigma-Aldrich, Germany) diluted to 1 ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were plated (2 ×106 cells per 100 mm plate) and grown for 24 h before transfection with Polyethylenimine (PEI, Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Microbial counts were determined by serial dilution plating of the homogenates on YESCA agar plates supplemented with 50 µg/mL of Congo Red (CR) (Sigma). The YESCA CR plates were incubated at 25°C for 2 days to induce curli production and the number of red colonies that showed curli expression were counted.
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bioRxiv - Plant Biology 2022Quote: ... 30 seedlings of each genotype were transferred to 24 well-plates containing 1 ml 0.5X MS supplemented with 50 μM MG132 (474787, Sigma-Aldrich). Plates were sealed with surgical tape and placed on a shaker at 55 rpm for 16 h at 23°C under continuous light ...
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bioRxiv - Developmental Biology 2022Quote: ... Smith’s laboratory) mouse ESC lines were routinely cultured without feeders on gelatin-coated plates (0.2% gelatin, Sigma-Aldrich, cat. G1890). Media were changed every 2 days and cells were passaged when approaching confluency (every 2-3 days) ...
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bioRxiv - Biochemistry 2022Quote: ... Then 96 individual CFUs per biological replicate were resuspended in 100 µl of liquid YPD supplemented with 30% glycerol in a 96 well plate (NuncTM Sigma), as one colony/well ...
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bioRxiv - Immunology 2020Quote: ... 293T-ACE2 cells were seeded 24 hours earlier at 1.5 × 104 cells per well in 96-well white flat bottom plates (Sigma-Aldrich). Cells were spinoculated at 800xg for two hours and incubated for two hours at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Serial dilutions of the homogenates and nasal collection were plated on BG plates supplemented with ceftibuten (Sigma-Aldrich Cat. SML0037) 10 μg/ml.
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bioRxiv - Immunology 2020Quote: PBMCs were rested overnight in complete medium and seeded at 2 × 105 cells/well in MultiScreen HTS Filter Plates (Merck Millipore) pre-coated with anti-IFN-γ (clone 1-D1K ...
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bioRxiv - Immunology 2020Quote: ... IELs were plated in 48-well plates and incubated at 37°C with 100ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma) and 200ng/mL ionomycin (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... cells were thoroughly resuspended and added to wells of a 96-well flat bottom tissue culture plate that had been previously coated with poly-L-Lysine as per manufacturer’s instructions (Sigma-Aldrich). As mentioned above GFP positive HIV infected cells were enumerated by fluorescence microscopy using the high content CYTELL imaging platform (GE Healthcare) ...
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bioRxiv - Neuroscience 2019Quote: ... coated glass slides in a 12-well plate O/N and then were stressed with 0.25 mM Sodium Arsenite (NaArs, S7400-100G Sigma-Aldrich) for 15 min ...
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bioRxiv - Cell Biology 2019Quote: ... and then 50 ul of the preincubated mixture was transferred to the assay plates after addition of 50 ul of actinomycin D at a final concentration of 1ug/ml (Sigma). Incubation of the assay plate was continued for 18 h followed by addition of 20 μl of a MTT Reagent (R&D) ...
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bioRxiv - Neuroscience 2020Quote: ... were formed by loading 12,000 iPSCs per well into 96-well plates pre-coated with Pluronic acid (1%, F-127, Sigma-Aldrich) and maintained in media consists of Glasgow-MEM supplement with 20% KSR ...
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bioRxiv - Molecular Biology 2020Quote: HEK293T cells were seeded on 12-well plates with glass coverslips (Marienfeld Superior) coated with poly-L-lysine (Sigma-Aldrich) one day prior to transfection ...
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bioRxiv - Neuroscience 2021Quote: ... or glass coverslips in 24-well plates (for immunocytochemistry) coated with Poly-D-Lysine (5 μg/ml, PDL, A-003-E; Millipore) and laminin (1 mg/ml Sigma L2020) ...
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bioRxiv - Microbiology 2021Quote: ... The pegged lid was then transferred to a new plate containing 180 μl of 0.1% crystal violet (Millipore Sigma, Canada), stained for 20 min at room temperature with gentle agitation ...
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bioRxiv - Neuroscience 2020Quote: ... we recovered the worms from starvation on plates pre-seeded with OP50 containing All-Trans Retinal (100 μg/ml, Sigma). Following the recovery period ...
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bioRxiv - Immunology 2021Quote: ... and 8 were transferred into a 96-well round-bottom plate and washed twice with FACS buffer (PBS + 0.02% sodium azide (Roth, Germany) +2 mM EDTA (Sigma-Aldrich) +2% h.i ...
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bioRxiv - Neuroscience 2021Quote: ... 1mL of HEK 293F cells from each batch was mixed together in a 12-well Corning Costar Not Treated Plate (Millipore) and incubated at 37°C with shaking on Orbi-Shaker (Benchmark ...
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bioRxiv - Neuroscience 2020Quote: ... Cortical neurons were plated in 6-well plates on coverslips coated with 100μg ml-1 poly-D-lysine (Sigma- Aldrich) and incubated at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... the viral supernatant was harvested and used to transduce 25,000 mouse primary keratinocytes seeded in 48-well plates in the presence of 8 µg/ml polybrene (Sigma Aldrich). The next day ...
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bioRxiv - Neuroscience 2020Quote: DU145 (5,000 cells/well) and DU145 Rho0 (20,000 cells/well) were grown on 96 well culture plates with doxycycline (Sigma 9891) concentrations ranging from 0 to 25 µM ...
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Molecular architecture determines brain delivery of a transferrin-receptor targeted lysosomal enzymebioRxiv - Neuroscience 2021Quote: ... The digested samples were spun at 3,364xg for 5 minutes and samples were transferred to a cellulose acetate filter plate (Millipore, MSUN03010) and spun at 3,364xg for 5 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... cells were approximately 80% confluent and passaged at 1:4 from 12-well plates to 15µg/mL Poly-L-Ornithine (Sigma-Aldrich) + 10µg/mL Laminin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... individual positive cells were sorted into the inner wells of a 96-well plate with 20 μl of a pre-reverse transcription (RT) lysis mix containing 0.1 μl NP40 (Sigma-Aldrich), 0.5 μl RNAse Inhibitor (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl of an overnight culture of OD600 = 5 suspended in water was spotted onto LB without NaCl agar plates containing the dye Congo red (40 μg/ml, Sigma) and Coomassie Brilliant Blue G-250 (20 μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... in either 4-well plates or P35 dishes (ThermoScientific) precoated for 4 h with 50 μg/ml poly-D-lysine (Sigma). Neurons were plated in DMEM containing 10 unit/mL penicillin ...
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bioRxiv - Neuroscience 2021Quote: ... and re-plated onto 13 mm glass cover slips in 24 well plates coated with 100 μg/ml Poly-L-Lysine (Sigma) and 1 μg/ml Laminin (Sigma ...
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bioRxiv - Immunology 2020Quote: ... For obtaining macrophage monolayer THP1 cells with required cell density (3×106 cells in T-25 flask and 4×104 cells per well in a 96 well plate) were treated with 20ng/ml of PMA (P8139, Sigma) and after one day PMA was washed off 2 times with RPMI ...
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bioRxiv - Neuroscience 2021Quote: ... Synchronized feeding third instar female larvae were sorted and placed in a hole created at the centre of an agar plate (3.3% wt/vol) filled with yeast paste containing 4% (w/v) FD&C Blue dye # 1 (Sigma Aldrich). The larvae were allowed to feed in the dyed-yeast paste for 2 hours ...
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bioRxiv - Neuroscience 2020Quote: ... 50’000 cells were seeded in each well of white clear bottom 96-well plates (triplicates for each construct) coated with poly-L-ornithine (Sigma). Cells were reverse transfected with 3 to 25 ng receptor vector and ~200 ng combined reporter vectors per well using polyethylenimine (Polysciences) ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions were monitored by thin layer chromatography (TLC) separation on pre-coated silica gel 60 F254 glass-supported plates (Millipore-Sigma). The TLC plates were visualized under UV light then stained using p-anisaldehyde/sulfuric acid solution and gentle heating ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were plated (2 x 107 cells per 150 mm plate) and grown for 24 h before transfection with Polyethylenimine (PEI, Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Experiments required the preparation of 24-well plates coated with a 1mg/ml poly-D-lysine hydrobromide (Sigma-Aldrich, P0899) solution for two hours and washed off with distilled water before cell plating ...
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bioRxiv - Physiology 2021Quote: ... day-1-adults were transferred on NGM OP50 plates either containing the solvent 0.25% dimethyl sulfoxide (DMSO) alone as a control or cycloheximide (Sigma #C7698) dissolved in 0.25% DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... and then 5,000 neurons/well were plated onto a 96 well plate (TPP) coated with (poly-L-lysine PLL, 0.01%, Sigma-Aldrich) and either laminin (10 μg/mL ...
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bioRxiv - Genetics 2022Quote: ... and 100k cells were seeded into each well of a 96-well plate coated with poly-L-lysine (Sigma, P4832).
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bioRxiv - Microbiology 2022Quote: ... Nine biological replicates (n = 9) of seven-day-old seedlings were transferred to ⅕ MS agar plates supplemented with 0.1 mM serotonin (Sigma-Aldrich) or with purified H2O ...
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bioRxiv - Microbiology 2022Quote: ... A 2.5- or 5-μl volume of ice-cold extracts were spotted along the bottom of polyethyleneimine-cellulose TLC plates (Millipore). The spots were air dried ...
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bioRxiv - Microbiology 2022Quote: ... for 5 minutes at room temperature in individual wells of a 24-well tissue culture plate filled with 500 µL 20% sodium dodecyl sulfate or 20% sodium deoxycholate (Sigma). After disc placement ...
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bioRxiv - Microbiology 2022Quote: ... cells were replated in 6-well plates with RPMI medium containing 1mg/mL G-418 (Sigma-Aldrich, St. Louis, MO). As we described previously (52) ...
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bioRxiv - Microbiology 2022Quote: ... were seeded in a 24 well plate pre-coated with 0.1 mg/mL poly-L-lysine (P9155-5MG, Sigma-Aldrich) and 24 hours later used for transfection ...
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bioRxiv - Neuroscience 2022Quote: ... culture media was aspirated from the plates using a Biotek EL406 plate washer and lysed with 13 μL of lysis buffer containing 0.5% Na-deoxycholate (Sigma Aldrich), 0.5% Triton-X (Sigma Aldrich ...