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Citations for Millipore Sigma :
3201 - 3250 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... A total of 3×103 A549 or H460 cells per well were seeded in ultra-low attachment 24-well plates in DMEM/F12 medium (Sigma) containing B27 supplement (Gibco ...
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bioRxiv - Biochemistry 2019Quote: ... The detergent-solubilized cell lysates were incubated with anti-FLAG M2 affinity gel (30 µl per plate, Sigma-Aldrich, A2220) for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... For experiments with viral infection worms were injected into pseudocoelom -behind the terminal bulb of the pharynx and were immediately placed into droplet of M9 buffer placed on NGM plates with 50 μg/mL FUdR (Sigma), seeded with OP50-1 E ...
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bioRxiv - Cell Biology 2019Quote: ... the cells were split to a 96-well plate in the same media and subsequently treated with (-/+) 10 nM water-soluble 17BE2 (Sigma) for 24 hours ...
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bioRxiv - Bioengineering 2020Quote: Mature brain organoids at 1 to 3-months of age were incubated on polyethyleneimine (PEI) pre-treated MEA plates with 10 μg/mL laminin (Sigma) for 4 hours to ensure attachment to the plate ...
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bioRxiv - Immunology 2019Quote: Cryopreserved PBMCs from healthy individuals in both cohorts were thawed and cells were transferred to 96-well deep-well plates (Sigma), resuspended in 25 μM cisplatin (Enzo Life Sciences ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plates were then washed again and incubated with horseradish peroxidase-conjugated rabbit anti-horse IgG (1:1000; Sigma, UK) for 3 hours at room temperature ...
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bioRxiv - Immunology 2021Quote: ... single-cell suspensions were prepared from spleens of mice and plated in Millipore MultiScreen-HA 96-well filter plates (Millipore) pre-coated with anti-mouse IL-2 ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St Louis, MO, USA) was added ...
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bioRxiv - Cell Biology 2021Quote: ... neurons were cultured in 24-well plates on 13 mm coverslips coated with 1 mg ml−1 poly-D-Lysine (Sigma) and 1 µg ml−1 laminin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Harvested medium from 12 plates per transfection or 18 plates of non-transfected cells was centrifuged 20min at 300g and concentrated with Centricon plus-70 filters (Millipore) with a 100KDa molecular weight cut-off ...
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bioRxiv - Cell Biology 2021Quote: ... Serum-free culture medium was added to the wells of the transwells plate while culture medium containing 10% FBS and 20 ng/mL EGF (Sigma) was added to the top of the matrigel ...
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bioRxiv - Immunology 2021Quote: ... plates were washed twice with PBS and permeabilization buffer (0.1% BSA [VWR, #0332], Saponin [Sigma, 47036-250G-F] in PBS) was added to permeabilized cells for at least 20 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... “wash III” (500 μl 100 % acetonitrile, and the “on-bead-digestion” plate (100 μl 20 mM Tris pH 8.5, Sigma-Aldrich 10708976001 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were plated (2×107 cells per 150 mm plate) and grown for 24 h before transfection with Polyethylenimine (PEI, Sigma). After 72 h ...
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bioRxiv - Microbiology 2021Quote: ... in 6 well plates were transfected with HSIV-vif plasmids using Fugene 6 or X-tremeGENE 9 DNA transfection reagent (Roche/Sigma). At 48 hour post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... the plates were washed 5 times with 200 μL of 1x PBST 100 μL of pNPP (Sigma, Cat. No. N2770) was added to each well ...
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bioRxiv - Immunology 2021Quote: 10 μg/ml of a purified RBD (not SpyTagged) in 0.1 M NaHCO3 pH 9.8 was coated onto Nunc® MaxiSorp™ 384-well plates (Sigma) and stored overnight at 4°C ...
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bioRxiv - Physiology 2021Quote: ... 6 × 105 cells were seeded on 6-well multiwell plates and counted every two days from day 4 to day 14 using Trypan blue (Sigma) and a Neubauer chamber.
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bioRxiv - Molecular Biology 2021Quote: ... 10 µL of stationary phase cultures of the complemented strains were spotted in BHI agar plates supplemented with Congo Red (Sigma) 25 µg.mL-1 and IPTG 1 mM ...
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bioRxiv - Immunology 2021Quote: ... with 1% milk and incubated for 2 hours with the mixture of antigen-coupled microsphere beads in 96-well filter bottom plates (Millipore). After washing with assay buffer ...
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bioRxiv - Neuroscience 2021Quote: ... On day 0 of organoid culture, iPS cells (passage < 50, approximately two thirds confluence) were dissociated from plates by EDTA (Sigma) treatment and plated in ultra-low-binding plates (Corning ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were seeded in triplicate at a clonal density of 10 cells/μl in 6 well (2 ml/well) ultra-low attachment culture plates (Coster) in serum-free medium supplemented with 20 ng/ml EFG (Sigma), 20 ng/ml FGF2 (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... 2001) from the transgene nEx2917 (glr-2p::hiscl1::sl2::mcherry) were transferred to plates containing 10 mM histamine dihydrochloride (Sigma) and assayed over a 3-hour interval starting 1 hour after exposure to histamine ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were seeded at a density of 0.4×106 cells/well in 24 multiwell plates coated with collagen (Collagen, C3867, Sigma Aldrich), grown over night at 34°C ...
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bioRxiv - Microbiology 2021Quote: ... A volume of 0.4 μL of this suspension was loaded onto the MALDI target plate and immediately overlaid with 1.2 μL of Norharmane matrix (Sigma-Aldrich) solubilized in chloroform/methanol (90:10 v/v ...
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bioRxiv - Cancer Biology 2020Quote: ... Sorted 96-well plates were frozen at −80 C until processing when cells were lysed with 0.2 µL 1 M KCl (Millipore Sigma), 0.2uL 10% Triton X-100 (Millipore Sigma) ...
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bioRxiv - Developmental Biology 2020Quote: ... plates of 3 mL ASW+BSA were supplemented with 1:1000 dilution of U0126 stock solutions (Sigma Cat. #662005-1MG) dissolved in DMSO to produce the indicated concentrations of U0126 ...
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bioRxiv - Developmental Biology 2021Quote: Live animals were removed from multi-well plates with a glass Pasteur pipette and anesthetized with 0.04% MS-222 (Sigma #A5040) in individual 35mm petri dishes containing a 14mm glass coverslip (MatTek Corporation #P35G-1.5-14-C) ...
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bioRxiv - Cell Biology 2020Quote: ... used for plasmid construction and propagation were cultured in Luria- Bertani (LB) broth or agar plates containing ampicillin (50 μg/ml, Sigma) as appropriate.
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bioRxiv - Immunology 2020Quote: ... Plates were blocked with 150µl of 10% skim milk in PBS for merozoites or 1% casein in PBS (Sigma-Aldrich) for MSP2 for 2 hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St Louis, MO, USA) was added ...
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bioRxiv - Immunology 2020Quote: For the proliferation assay splenocytes were plated in a Corning Costar 96-well round bottom cell culture plate (Sigma-Aldrich) at a cell density of 2.5 × 105 cells in a final volume of 170 µL ...
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bioRxiv - Microbiology 2020Quote: ... 198 μl/well were transferred into 3 wells of a black 96-well plate with transparent flat bottom (Sigma Aldrich). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... and plated at a density of 0.5 × 106 cells per well in six-well plates coated with poly-D-lysine (Sigma-Aldrich). Neuronal cultures were maintained at 37 °C with 5% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cardiac myocytes were then plated at a density of 0.5 × 106 cells per well on 34.8 mm plastic plates that had been pre-treated with 5 μg/mL fibronectin (cat# F1141, Sigma-Aldrich) in serum free DMEM/F12 medium (cat# 11330032 ...
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bioRxiv - Immunology 2021Quote: ... OAS1 KO 293T cells were seeded in 12 well plates coated with 10 μg/mL poly-L-ornithine hydrobromide (Sigma) and allowed to adhere overnight ...
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Re-investigating the coughing rat model of pertussis to understand Bordetella pertussis pathogenesisbioRxiv - Immunology 2021Quote: ... Serial dilutions of the homogenates and nasal collection were plated on BG plates supplemented with ceftibuten (Sigma-Aldrich Cat. SML0037) 10 µg/ml to decrease growth of normal rat respiratory tract flora.
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bioRxiv - Immunology 2020Quote: ... Organs were dissected in cold PBS and placed in 24-well plates with 500μL cold digestion buffer composed of PBS with 1mg/mL collagenase D (Sigma 11088882001), 100 U/mL DNaseI (DN25-100mg ...
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bioRxiv - Microbiology 2021Quote: ... CFUs were determined by homogenising organs in 1 mL sterile PBS and plating serial dilutions on Salmonella-Shigella agar plates (SIGMA). Plates were incubated overnight at 37°C before counting of colonies.
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bioRxiv - Immunology 2021Quote: The S-variant and RBD coupled bead sets were incubated with sera from immunized mice at 4 dilutions (1:100; 1:1000; 1:10000; 1:100000) for 1 h in 96-well filter plates (MultiScreenHTS; Millipore) and analyzed on a Luminex analyzer (Luminex / R&D Systems ...
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bioRxiv - Microbiology 2021Quote: ... The ELISA plates were washed four times with PBST and then 100 μl of 2,000-fold diluted anti-strep-HRP (Millipore Sigma) was added into each well for another 1 hr at 37□°C ...
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bioRxiv - Immunology 2020Quote: ... grown at the log-phase in 6-well plates in the presence of DMEM supplemented with 1% FBS and 0.5% (w/v) methylcellulose (Sigma-Aldrich). After 2 d of incubation at 37°C ...
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bioRxiv - Immunology 2020Quote: ... The number of viable BCG in stocks was determined by serial dilution and plating on 7H11 Middlebrook plates (Sigma-Aldrich), containing OADC supplement (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... were seeded at 200,000 cells/well of 6 well plates and transduced with lentiviral particles at final MOI of 0.5 with 8 µg/ml polybrene (Sigma-Aldrich). Transduced cells were selected in 10 µg/ml puromycin (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated onto the 96-well plates coated with poly-L-ornithine (Sigma-Aldrich, Merck KGaA, St. Louis, MO). Equal volumes of cell suspension were plated onto the center of the dish ...
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bioRxiv - Genetics 2020Quote: Serial ten-fold dilutions of logarithmic yeast cells were spotted on fresh Synthetic Dextrose (SD)-complete (or SD lacking a specific amino acid to preserve the plasmid) plates with or without different concentrations of Methyl methane sulfonate (MMS)(Sigma) and incubated at 30°C for three days ...
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bioRxiv - Microbiology 2021Quote: ... Thereafter 10 μl of the culture was streaked on SA (1% lactose or glucose) agar plate supplemented with 10 μg/ml 5-fluoroorotate (Sigma). Plates were incubated at 30°C until 5-fluoroorotate resistant colonies emerged ...
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bioRxiv - Immunology 2021Quote: ... total mononuclear cells isolated from the epithelium were plated in 48-well plates and incubated at 37° C with 100ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma), 200ng/mL ionomycin (Sigma ...
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ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... of 106 cells/ml/well in a 12-well plate with fresh media or with fresh media supplemented with 1000 ng/ml LPS (Sigma) for 6 hrs ...