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Citations for Millipore Sigma :
3051 - 3100 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Cell lines cultured in 6 well plates in vitro were washed with ice-cold PBS on ice and incubated in 250 μL of RIPA buffer (Sigma) containing protease (Roche ...
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bioRxiv - Cancer Biology 2021Quote: 105 HCT116 cells as well as HCT116 cells with acquired oxaliplatin resistance and HCT116 cells with BOLD-100/KP1339 resistance were seeded (N = 4 for each respectively) into 12-well plate (StarLab) with 2 mL McCoy’s 5A medium (Sigma-Aldrich) containing 10% FCS (BioWest ...
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bioRxiv - Cancer Biology 2021Quote: ... and HCT116 cells with acquired BOLD-100/KP1339 resistance were seeded as 2 × 105 cells/well in 12-well plate formate in 1 mL McCoy’s medium (Sigma Aldrich) supplemented with 2 mm glutamine and 10% FCS ...
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bioRxiv - Cancer Biology 2021Quote: HDMB03 cells (3×105 in 6-well plates) were treated with global de-methylating agent 5-AzaC (5 μM) (5-Aza-2-deoxycytidine; Sigma). Following 96 hours of incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... In some cases mononuclear cells were cultured as mammospheres in 6 cm culture plates coated with polyhydroxyethylmethacrylate (12 mg/ml, Sigma), under hypoxic conditions (7% O2 ...
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bioRxiv - Cancer Biology 2019Quote: Cells cultured at 37 °C in 12-well plates were incubated in the dark for 75 min with culture medium containing 0.5 mg ml-1 MTT (Sigma-Aldrich). The cells were then carefully washed with PBS once and incubated for 10 min in the dark in dimethyl sulfoxide with mild shaking ...
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bioRxiv - Cell Biology 2021Quote: Hek293T cells were plated in 24-well plates on cover glass (Carl Roth) coated with poly-L- Lysine (Sigma-Aldrich). Cells were transfected with 125 ng of plasmid and total plasmid levels were adjusted to 500 ng with empty plasmid ...
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bioRxiv - Cell Biology 2021Quote: Cells were grown to early log phase in synthetic complete (SC) media + 2% glycerol + 1% ethanol and gently sonicated before being loaded into a CellASIC Y04C microfluidics plate (Milipore SIGMA) under continuous media flow at 2 psi ...
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bioRxiv - Neuroscience 2020Quote: ... After 2 h at RT plates were washed three times and incubated with anti-human Fab specific-HRP conjugated secondary antibody (Sigma) for 1 h at RT ...
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bioRxiv - Cell Biology 2021Quote: ... 2×105 cells were seeded in 6-well tissue culture plates and incubated at 37°C for 24 hours before exposure to 4 mM hydroxyurea (HU; Sigma) in regular growth medium for 5 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... Nucleofected cells were plated onto duplicate wells of Matrigel®-coated 6-well culture plates for 24 h before adding 2.5μg/mL puromycin dihydrochloride (Sigma-Aldrich) for selection ...
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bioRxiv - Microbiology 2020Quote: ... and 2a strains were grown at 37°C in Tryptic Soy Broth (TSB) with shaking or TSB agar plates supplemented with 0.01% Congo Red (Sigma-Aldrich) and Ampicillin (100 μg/ml ...
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bioRxiv - Plant Biology 2020Quote: ... thaliana seedlings used in the root-hair growth assays were grown vertically on plates containing 0.25x Murashige and Skoog (MS; Sigma-Aldrich) medium at pH 5.7 supplemented with 0.6% (w/v ...
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bioRxiv - Microbiology 2020Quote: The Congo red binding (CRB) ability of the APEC isolates was determined using agar plates supplemented with 0.003% CR dye (Sigma, USA) and 0.15% bile salts ...
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bioRxiv - Microbiology 2020Quote: ... for ELISpot detection in combination with multiscreen immunoprecipitation (IP) ELISpot polyvinylidene difluoride (PVDF) 96-well plates (Merck Millipore, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... 5-10 L4 worms of each strain were transferred onto RNAi plates (containing 2 mM IPTG and 25 mg/mL carbenicillin) or RNAi plates containing 15 μg/mL Doxycycline (Dox, Cat. D9891, Sigma) seeded with E ...
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bioRxiv - Microbiology 2020Quote: ... OD600nm adjusted to 0.1 (measured in 100 µL volume in a clear flat bottom 96 well plate) and supplemented with 1.6 mg/ml Red#40 dye (Sigma, Israel). Fifty µL of this culture were pipetted to rows A-G of the plate containing the amoeba ...
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bioRxiv - Plant Biology 2021Quote: ... lipid extracts were roughly separated into polar and non-polar lipids by thin-layer chromatography (TLC) using silica plates (TLC silica gel 60, Merck Millipore) developed with chloroform because the high amount of shikonin derivatives hampered the chemical analysis of TAG and polar lipids by LC-MS ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed two times and horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (Sigma Aldrich, St. Louis, MO) was used at a 1:5,000 dilution and incubated at room temperature for 1 hour ...
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bioRxiv - Plant Biology 2021Quote: ... After a 10 min centrifugation step (15,000 x g) the samples were filtered in a 96-well MultiScreen filter plate (0.65 μm, Millipore, Burlington, MA). Reverse-phase liquid chromatography was performed using a DionexUltimate 3000 Series LC system (HPG-3400 RS High-Pressure pump ...
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bioRxiv - Microbiology 2020Quote: ... Pneumococcal isolates were grown in triplicate in BHI in 96-well plates in the presence of hydrogen peroxide (H202, Sigma) at concentrations 0 ...
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bioRxiv - Microbiology 2021Quote: ... through electroporation (2.5 kV, 5 ms) and the cells were spread on a Luria-Bertani (LB)-agar plate (Miller, Sigma-Aldrich) supplemented with 100 µg/mL ampicillin ...
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bioRxiv - Microbiology 2021Quote: 48-hour old biofilms were removed from agar plates using sterile loops and placed in 250 μl of BugBuster (Novagen). The biofilm was disrupted by repetitive passage through a sterile 23-gauge needle ...
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bioRxiv - Neuroscience 2020Quote: ... Cell culture plates (Denville Scientific, Inc., Plainfield, NJ) were coated overnight with poly-L-lysine (50 µg/mL; Sigma-Aldrich), supplemented with 7.5 µg/mL laminin (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2021Quote: ... samples were taken from the reactions and the soluble fractions were separated from the insoluble substrate particles using a 96-well filter plate (Millipore) operated with a vacuum manifold ...
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bioRxiv - Microbiology 2021Quote: ... The enriched CD138+ cells were incubated overnight in RPMI 1640 supplemented with 10% FBS in MultiScreen-HA Filter Plates (Millipore) pre-coated with SARS-CoV-2 S protein ...
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bioRxiv - Biophysics 2021Quote: ... counted and then plated in a Cell-bind 96-well plate at a density of 0.5 × 104 cells/well in 10 μM all-trans retinoic acid (RA; Sigma-Aldrich) in DMEM/F12 with 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... the plate was incubated with 50 µl of goat anti-rabbit IgG conjugated to alkaline phosphatase diluted 1:2000 (A3687, Sigma) in PBS for 1 h at RT ...
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bioRxiv - Microbiology 2019Quote: ... The extracted lipids were dissolved in 15 µl of chloroform and processed for TLC analysis using plastic backed silica gel 60F254 plates (Sigma) and a mobile phase system containing petroleum ether ...
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bioRxiv - Microbiology 2019Quote: ... The extract was spotted onto the TLC plate as follows: 7 μl of bacterial extract or 5 μl of 20 μM PQS synthetic standard (Sigma). PQS quantity was then determined by exposing resolved TLC plates to 325 nm UV light ...
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bioRxiv - Microbiology 2020Quote: ... Adjusted cultures were 1:10 serially diluted 5-times and 5 µl of each dilution spotted on YPD agar plates containing indicated concentrations of caspofungin (Sigma). Plates were incubated at 30°C for 3 days and photographed ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... coli DH5α cells were transformed with the resulting pho-lac fusion plasmids and streaked on dual indicator plates containing LB agar with 5-bromo-4-chloro-3-indolyl phosphate disodium salt (X-Phos) (Sigma) at a final concentration of 80μg/ml and 6-chloro-3-indolyl-β-D-galactoside (Red-Gal ...
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bioRxiv - Neuroscience 2021Quote: ... Astrocyte cell line cultures were scraped off 6-well plates with ice-cold RIPA buffer with protease and phosphatase inhibitors (MSSAFE, Sigma) and transferred to a chilled microcentrifuge tube ...
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bioRxiv - Cancer Biology 2020Quote: Solubility assays were performed using Millipore MultiScreen®HTS-PFC Filter Plates designed for solubility assays (EMD Millipore, Billerica, MA). The 96-well plates consist of two chambers separated by a filter ...
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bioRxiv - Cancer Biology 2020Quote: ... Lenti293 packing cells were plated at 6 × 105 cells/well in six-well tissue culture plates and transfected with pLKO.1-puro lentiviral empty vector or shCCM1 vector (Sigma) using X-treme GENE HP DNA transfection Reagent (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: ... were seeded in 6-well plates before addition of fresh medium containing viral supernatant (~5×105 transducing units) and Polybrene (8 μg/mL; Sigma) and ‘spin-oculated’ for 90 min at 1,500 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... Cells on the surface of the plate were then lysed in the Triton lysis buffer (1% TritonX-100 [Sigma-Aldrich T8787] ...
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bioRxiv - Cell Biology 2021Quote: ... GMPS were sorted as described above from CD45.1 and cells were cultured in a 12 well plate pre-coated with human fibronectin (EMD Millipore) in RPMI1640 media + 10% Fetal Bovine Serum (FBS) ...
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bioRxiv - Cell Biology 2020Quote: ... were seeded in DMEM supplemented with 10 % FBS in 96 well plates incubated with increased concentrations of compound 2 and treated with 3-NP at 10 mM (#N5636, Sigma). Then ...
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bioRxiv - Cell Biology 2020Quote: ... cells were replica-plated onto pABA− (2% glucose, w/v) plates with 0 µM or 25 µM linolenic acid (C18:3, Sigma). Colonies that grew on 25 µM linolenic acid were picked into YEPD overnight cultures and struck on YEPD plates ...
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bioRxiv - Cell Biology 2021Quote: SK-N-SH-N cells were plated onto 6-well tissue culture plates coated with poly-L-lysine (Sigma-Aldrich). Cells were plated at 3×105 cells/ml in MEMα medium containing a very low-serum level (2% FBS) ...
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bioRxiv - Cell Biology 2021Quote: ... in polystyrene 96-well cell culture plates coated in 0.1% sterile gelatin (ES-006-B, Sigma Aldrich, St. Louis, MO). Approximately 50,000 cells were seeded for dense plating and 6,250 cells for sparse plating (1:8) ...
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bioRxiv - Cell Biology 2021Quote: NCMs were seeded at a density 4 or 8 × 105 cells/well in 6-well plates coated with 0.01% of collagen (Sigma-Aldrich) and cultured in a medium containing DMEM/Medium199 (4:1) ...
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bioRxiv - Bioengineering 2022Quote: ... HeLa cells were seeded at 25% confluency in 96 well plates and transduced with virus (lentiCRISPR-v2 with or without HPRT1 guide) and 8 μg/mL polybrene (Millipore). Virus was removed the next day and 2.5 μg/mL puromycin was added to remove cells that did not receive virus two days later ...
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bioRxiv - Cell Biology 2022Quote: ... and 3 mL volumes of culture were transferred per well to 6-well tissue culture-treated polystyrene plates (Sigma CLS3506) and incubated at 23°C for 48 hours ...
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bioRxiv - Cancer Biology 2022Quote: Leukemia cells were seeded at a density of 2.5 x 105 cells/well of a non TC-treated 12-well plate in complete medium containing polybrene (10µg/mL, EMD Millipore), and then transduced with lentivirus by centrifugation at 2,500rpm for 90 minutes at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... 70,000-100,000 LECs/well were plated on Matrigel-coated 48-well-plates or Millicell EZ 8-well glass slides (Millipore, LabTek). LECs were allowed to adhere and form tubes for 4-6 hours before medium was aspirated and small intestinal crypts or single cells from organoids were plated atop in 20µl Matrigel domes mixed with organoid media.
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bioRxiv - Cell Biology 2022Quote: ... 50,000 MBS-MEFs were seeded in 6-well plates and exposed to the virus particles mentioned above in serum-free media supplemented with 6 µg/ml polybrene (Sigma). Cells with high or low expression of tdTomato were selected by FACS (BD Biosciences Aria III).
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bioRxiv - Biochemistry 2022Quote: ... coli strain MG1655-seeded NGM plates in the presence or absence of 1 mM auxin (IAA, indole-3-acetic acid, Sigma).
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bioRxiv - Bioengineering 2022Quote: THP-1 cells plated at a seeding density of 35000 cells/well in a 96-well plate were activated with 20 ng/mL phorbol 12-myristate 13-acetate (Sigma) and incubated ...