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Citations for Millipore Sigma :
3351 - 3400 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 3533-005-02) and overlaid with Organoid Media (Supplementary file 1) in 6-well ultra-low adhesion plates (Sigma, CLS3471). H2B:GFP retrovirus (91 ...
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bioRxiv - Plant Biology 2023Quote: ... 125 μL of the 150 μL solution of stained biofilm were transferred into a well of a polyvinyl chloride 96-well flat-bottom plate (Catalog # CLS2595, Sigma), for absorbance assessment at 550 nm in a microplate reader (SpectraMax® i3x (Molecular Devices) ...
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bioRxiv - Neuroscience 2023Quote: iPSC-derived microglia were seeded at a density of 5×104 cells per well in microglia differentiation medium in 96-well plates and stimulated with 1.0 µg/ml LPS (Sigma, #L4391) in microglia differentiation medium ...
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bioRxiv - Synthetic Biology 2022Quote: ... All yeast transformations were plated on synthetic complete (SC) plates containing 1.7 g/L yeast nitrogen base without amino acids and ammonium sulphate (Sigma Aldrich), 1 g/L monosodium glutamate (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... and 3dpi xenografted larvae were anaesthetised in 1x Tricaine and embedded in a 96-well ZF plate (Hashimoto Electronic Industry) with 0.5 % ultra-low gelling agarose (Cat# A2576-25G, Sigma-Aldrich) for automated imaging on a high-content imager (Operetta CLS ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 DL of the supernatants containing 32P labeled intracellular nucleotides were resolved on a 20 x 20 cm plastic PEI cellulose F TLC plate (Millipore) using 1.5 M KH2PO4 pH 3.4 as a solvent ...
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bioRxiv - Pathology 2023Quote: ... placed in a 96-well plate (6267170, Buch & Holm) and digested in normal growth media containing 1.42 mg/ml Collagenase (C6885, Sigma Aldrich) for 6 hours at 37ᵒC ...
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bioRxiv - Molecular Biology 2022Quote: ... Blastocysts collected 96 h after hCG injection were transferred to single wells of culture plates coated with 0,2% gelatin (Sigma-Aldrich) with feeder layer of inactivated MEFs ...
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bioRxiv - Biochemistry 2023Quote: ... and the supernatant was filtered at 300 × g for 3 minutes at 4°C through a 0.45-μm 96-well filter plate (Millipore, MSHVN4550) to remove protein aggregates ...
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bioRxiv - Microbiology 2023Quote: ... Plates were washed again and developed with 50 μl/well of 1 mg/ml para-Nitrophenyl Phosphate (pNPP) substrate (Sigma), dissolved in glycine buffer (0.1M glycine ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial cultures were then diluted 1/100 and grown at 370C for 12 hours in 96-well microtiter plates (16 wells for each strain) in the absence or presence of a range of concentrations of trimethoprim (Sigma). 600 nm OD data were collected at 10 min intervals ...
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bioRxiv - Immunology 2022Quote: ... with 1% milk and incubated for 2 hours with the mixture of antigen-coupled microsphere beads in 96-well filter bottom plates (Millipore). After washing with assay buffer ...
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bioRxiv - Microbiology 2023Quote: ... were transfected into BHK21 cells along with Nucleocapsid and Spike expression vectors (0.5 μg each) in 24-well plate using X-tremeGENE 9 DNA transfection reagent (Sigma Aldrich) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... RPL28 mutants were generated by growing BG14 to saturation overnight in YPD medium and plating onto YPD plates containing 5 µg/mL of cycloheximide (Sigma). Colonies were purified on YPD plates and screened by PCR-seq for mutations in RPL28 using primers listed in Sup ...
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bioRxiv - Developmental Biology 2023Quote: ... while all exit and differentiation experiments were performed in plates coated with 10μg/ml laminin in PBS for at least 30 min at 37°C (Sigma Millipore CC095). For all experiments ...
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bioRxiv - Cell Biology 2023Quote: ... cells were grown overnight in SC-URA medium with 2% dextrose and were plated in SC-URA plates in the presence of 2% dextrose (Sigma) or 2% galactose (Difco) ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed a final time (5x with PBS-T) and the plate was allowed to develop by addition of AP substrate (Sigma). Optical density at 405 nm was measured in 5 min intervals ...
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bioRxiv - Microbiology 2023Quote: ... The indexed PCR products were again normalized by loading 15 µl indexed PCR products onto Sequal Prep TM Normalization plates and were pooled and concentrated using a Amicon® Ultra 0.5 ml centrifugal filter (30K membrane, Merck Millipore). A NanoDrop™ One Microvolume Spectrophotometer (Thermo Scientific™ ...
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bioRxiv - Cell Biology 2023Quote: ... Poly D-lysine treatment was performed by coating plates with poly D-lysine hydrobromide (0.1 mg mL−1 in H2O from lyophilized powder, Sigma-Aldrich) for 15 min ...
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bioRxiv - Genomics 2023Quote: ... or cell type (roughly 5X106 iPSC-NPCs or 1X106 iPSC-neurons on a 6-well plate, enzymatically detached with Accutase (Millipore) and pelleted with centrifugation for 5 mins at 1,000 G) ...
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bioRxiv - Cell Biology 2023Quote: ... in antibiotic-free media at 2 M cells per well of a 12-well plate that had been coated with 0.1% gelatin (Sigma, G2500) for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... 400 µL of growth media (acclimated in incubator 1-hour prior) was added to each nucleovette and cells transferred to plates pre-coated with poly-D-Lysine (Sigma). Cells were left to incubate for 24-hours ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were typically grown on NA plates or in Luria Bertani (LB) Lennox broth with low salt (Sigma-Aldrich L3022), at 25°C ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% FBS were seeded in each well of a 96 well MultiScreen® HV filter plate (EMD Millipore) placed on top of a 96 well collection plate ...
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bioRxiv - Cancer Biology 2023Quote: ... Cas9-expressing PDXTs were seeded at 5,000 cells/well in 96-well plates in the presence of blasticidin and 2 µg/ml puromycin (Sigma Aldrich) and cultured for seven days ...
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bioRxiv - Physiology 2023Quote: ... GLP-1 content in the supernatant of T0 and T20 samples was assessed using a Glucagon-Like Peptide-1 (GLP-1) Total ELISA KIT (96-Well Plate Cat. # EZGLP1T-36K, EZGLP1T-36BK, Millipore).
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bioRxiv - Biochemistry 2023Quote: ... Resuspended lipids from each sample were spotted on high-performance thin-layer chromatography silica plates (Millipore Sigma, Burlington, MA, USA) and separated using a solvent system containing hexane:isopropyl ether:acetic acid (65:35:2 [vol/vol/vol]) ...
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bioRxiv - Bioengineering 2023Quote: ... 10 μl of cell lysate was transferred to a 96-well clear assay plate and made up to 100 ul with 90 μl phosphatase substrate (Sigma) in 1.5 M alkaline buffer solution (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... a dilution series of 107 to 103 cells/ml were plated in 5 μl spots on M9 minimal media plates with 10 mM glucose or 10 mM glucose-6-phosphate (Sigma) and antibiotics ...
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bioRxiv - Molecular Biology 2023Quote: Plants were started on MS plates composed of 1X Murashige and Skoog basal salt medium (Sigma-Aldrich, St. Louis, MO) at pH 5.7-5.9 with 0.8% Type I micropropagation agar (Spectrum Laboratory Products ...
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bioRxiv - Neuroscience 2023Quote: ... which were allowed to swim out in a 24-well plate containing approximately 0.5 ml of warmed Tyrodes’ solution (Sigma, USA) per well ...
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bioRxiv - Neuroscience 2023Quote: ... and plated at a density of 0.5 × 106 cells per well in six-well plates coated with poly-D-lysine (Sigma-Aldrich). Neuronal cultures were maintained at 37 °C with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... 0.4 × 106 CXCR3A-transfected CHO cells/ml or 60 000 HMVEC/ml (2ml/well) were seeded in flat bottom 6-well plates (2.0 ml/well; TPP, Sigma-Aldrich) in supplemented Ham’s F-12 growth medium + 10% (v/v ...
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bioRxiv - Plant Biology 2023Quote: ... Seedlings were then transferred to 1.0% (w/v) GM agar plates supplemented with/without indicated concentrations of ABA (Sigma-Aldrich) or sorbitol and grown vertically for an additional 7-11 days ...
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bioRxiv - Cell Biology 2023Quote: ... Agonist or vehicle was added to cells grown in 12-well plates coated with 0.001% (w/v) poly-L-lysine (Millipore Sigma) and incubated at 37 °C and 5% CO2 as indicated in figure legends ...
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bioRxiv - Immunology 2023Quote: ... Plates were then incubated with 100 μL of substrate solution consisting of 4 mg o-Phenylenediamine dihydrochloride (OPD, Sigma, #P8787) dissolved in 10 mL filter sterilized citrate buffer (0.05M citric acid anhydrous ...
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bioRxiv - Microbiology 2023Quote: ... and splenocytes at 105 cells/well were added to 96-well high-protein-binding Immobilon-P membrane plates (Millipore, UK) that had been precoated with 5 µg/ml anti-IFN-γ monoclonal antibody (mAb ...
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bioRxiv - Biochemistry 2023Quote: ... the plates were incubated with 100 μL/well horseradish peroxidase-conjugated anti-mouse immunoglobulin second antibody (Sigma, St. Louis, MO) in Buffer D of pCa 9 or 4 at room temperature for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... the samples were further treated in a 96-well plate following the “Coenzyme A Assay Kit” protocol from Sigma-Aldrich. The absorbance of the colorimetric product (570 nm ...
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bioRxiv - Cancer Biology 2023Quote: ... 1×106 cells were plated on one well of 6 well-plate previously coated with 10μg/ml of laminin (Sigma, #L2020) in 1xPBS for 1 hour at 37C ...
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bioRxiv - Cancer Biology 2023Quote: Artc1-KO and Artc1-WT MEFs were incubated on 96-well plates (1×104 cells/well) for 24 h before exposure to H2O2 (0-100 µM, Sigma). Necrosis inhibitor IM-54 (0-10 µM ...
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Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... ELSIPOT assays were performed using a MultiScreen sterile 96-well filter plate with 0.45 um pore size of hydrophobic polyvinylidene difluoride (PVDF) membrane (Millipore S2EM004M99), antigen-presenting cells K562.hMR1 ...
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bioRxiv - Immunology 2023Quote: ... After a further 1 hour incubation at 37°C plates were washed and 50 μl 1:2000 diluted cross-adsorbed anti-mouse HRP (Sigma) was added to each well and incubated for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... compound plates were spun down at 5000g for 10 min and manually transferred to 384 MultiScreenHTS HV Filter Plates (0.45 µm, Millipore, #MZHVN0W10) and spun down again for 1 min at 500g into an empty compound plate ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting cells were then plated on Spec/Kan agar plates supplemented with 5% sucrose and 0.05% acetamide (Sigma-Aldrich), to induce msmeg_0317 deletion via the second crossover ...
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bioRxiv - Neuroscience 2023Quote: ... Pre-sterilized 6-well CytoView MEA plates were purchased from Axion Biosystems and coated with 0.5% polyethyleneimine diluted in HEPES (both from Sigma-Aldrich) and 20 μg/mL natural mouse laminin (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... wash and imaging buffers were kept in 96 well or 4 well deep plates covered in parafilm (Cat.# P7793, Sigma) and placed on the CNC bed ...
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bioRxiv - Cancer Biology 2022Quote: ... TCs and platelets suspensions were placed on p24 well plates containing glass coverslips previously treated with poly-L-lysine solution (Sigma). After TC-platelet suspension sedimentation (30 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: MDCK cells (2 x 105 cells) were cultured on polycarbonate Millicell culture plate inserts (12- mm diameter, 3-µm pore size; Merck Millipore) at 37°C under 5% CO2 atmosphere.
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bioRxiv - Biochemistry 2023Quote: ... Ninety-six randomly selected clones from this transformation were grown in 500 µL AIM supplemented with 50 µg mL−1 kanamycin for 48 hours at 20 °C in 96-deep well plates sealed with Breathe-Easier membranes (Sigma). The culture medium was then separated from the cells by centrifugation for 15 minutes at 4500 x G and 10 µL of culture supernatant was assayed for xylobiose lysis using the protocol detailed in Controls for the detection of CAZyme activities in coupled reactions.