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Citations for Millipore Sigma :
2951 - 3000 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Cells were routinely tested for free of mycoplasma by using LookOut Mycoplasma PCR detection kit (Sigma, #MP0035).
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: ... Blood DNA sample was prepared using Extract-N-Amp Blood PCR Kit (XNAB2R, Sigma, St. Louis, MO) and diluted 100 times ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were routinely tested for the mycoplasma absence using the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Microbiology 2023Quote: Classical PCRs were performed using the RedTaq (WVR) and amplicons were visualized by SYBR Green stained (Sigma) agarose gel electrophoresis (1 to 2% agarose) ...
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bioRxiv - Biochemistry 2023Quote: ... All cells were examined negative for mycoplasma infection using PCR-based Mycoplasma Detection Kit (Sigma, MP0035-1KT). Mouse embryonic fibroblast cell (MEF ...
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bioRxiv - Developmental Biology 2023Quote: DNA was extracted from mice tail samples using Extract-N-Amp™ Tissue PCR Kit (Sigma, USA). PCR primers used for genotyping are listed below ...
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bioRxiv - Microbiology 2023Quote: ... All the cell types were confirmed free of mycoplasma using Lookout Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... Cells were routinely tested for mycoplasma using the LookOut Mycoplasma PCR Detection Kit (cat#MP0035, Sigma-Aldrich).
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bioRxiv - Neuroscience 2023Quote: Cells in culture were routinely tested for mycoplasma infection with Lookout Mycoplasma PCR detection kit (Sigma, MP0035), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were PCR-amplified with 12-13 cycles using KOD Hot Start DNA polymerase (Millipore, 71086-3) and cleaned up using 1X KAPA Pure Beads ...
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bioRxiv - Molecular Biology 2024Quote: ... All cell lines were verified as mycoplasma free using the Lookout Mycoplasma PCR Detection Kit (Sigma-Aldrich) and genetic identities were validated by PCR ...
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bioRxiv - Neuroscience 2024Quote: DNA was extracted from mice tail samples using Extract-N-Amp™ Tissue PCR Kit (Sigma, USA). PCR primers used for genotyping are listed below ...
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bioRxiv - Cell Biology 2024Quote: ... Expression of mRNA was quantitatively assessed by real-time PCR using KAPA SYBR FAST (Sigma-Aldrich, KK4608) in a Biorad iCycler IQ (Biorad ...
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bioRxiv - Biophysics 2020Quote: ... Bacteria on plates were washed and dispersed in aqueous blank buffer containing 0.17% (w/v) yeast nitrogen base (Sigma-Aldrich) and 0.5% (w/v ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cells lysis was done in a 96 deep-well plate by adding 450µl of lysis buffer containing 1M sorbitol (SIGMA-ALDRICH), 100mM EDTA and 0.45µl lyticase (10IU/µl) ...
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bioRxiv - Microbiology 2020Quote: ... Plates were coated with 100 μl of 0.02% sodium carbonate (pH 9.6) containing 2 μg/ml fibronectin (Sigma-Aldrich, USA) or 100 μl of phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Dissociated neurons were resuspended in normal medium and plated in 24-well plates containing 12-mm glass coverslips pretreated with 50 µg/ml poly-L-lysine (Sigma) in borate buffer ...
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bioRxiv - Cancer Biology 2021Quote: Tail tip fibroblasts were seeded in 96-well plates and the mitochondrial membrane potential (MMP) was determined using the mitochondrial membrane potential kit (Sigma) following the recommendations made by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... single cell clones were detached using cell dissociation solution and re-plated in a larger fibronectin coated plate (Sigma-Aldrich) with SFM.
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bioRxiv - Immunology 2021Quote: ... The mix was transferred to a plate coated with avidin and the FLAG-peptide was detected with an anti-FLAG-alkaline phosphatase conjugate (Sigma), developed with pNPP SigmaFAST substrate and absorbance was read with a 405nm – filter (Figure 3D-F).
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bioRxiv - Immunology 2021Quote: ... a sandwich ELISA was used in which 1% Trixon X-100-treated virus lysates were added to plates pre-coated with ConA (50 µg/ml, Sigma) and reacted with anti-Env mAbs 66 ...
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bioRxiv - Immunology 2021Quote: ... MAb was reacted with antigen coated on the plates and then treated with serially diluted C1q from human serum (Sigma). C1q binding was detected with horseradish peroxidase-conjugated anti-C1q antibody ...
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bioRxiv - Immunology 2021Quote: ... Blood ASCs and BM plasma cell populations were cultured in cell-free MSC secretome media in 96-well flat bottom cell culture plates (Corning/Sigma) at 37°C in a humid ...
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bioRxiv - Cell Biology 2020Quote: ... neurons were cultured in 24-well plates on 13 mm coverslips coated with 1 mg/ml poly-D-lysine (Sigma) and 1 μg/ml laminin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... A 48-well MEA plate with 16 electrodes per well was precoated with 1 mg/ml polyethyleminine (PEI; Sigma Aldrich) for 1 hour at RT ...
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bioRxiv - Genetics 2021Quote: ... 96-well medium binding plates were coated with 100 μl/well of 2 μg/ml of KLH (H8283, Sigma-Aldrich), or MDP (A9519 ...
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bioRxiv - Microbiology 2020Quote: ... Stationary-phase cultures were diluted 1:10 000 into fresh 7H9 before spotting onto prepared 7H10 plates using a 96-well replicator pin (Sigma). All spotting was performed in triplicate ...
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bioRxiv - Microbiology 2021Quote: ... The plate was washed three times and 100 μl/well of SigmaFast o-Phenylenediamine dihydrochloride (OPD) developing solution (Sigma Aldrich) were added ...
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: The statistical effect size (Z-factor) was calculated for each plate (189 in total) using the mock-transfected vehicle (DMSO) or etoposide (Sigma) treated cells negative and positive control respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 g/L NaCl with additional 15 g/L agar for plates) supplemented with 100 µg/mL ampicillin (Sigma-Aldrich). PCR amplifications were performed with Q5 polymerase (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... Poly D-lysine treatment was performed by coating plates with poly D-lysine hydrobromide (0.1 mg mL−1 in H2O from lyophilized powder, Sigma-Aldrich) for 15 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5×106 cells were mixed in a well of a 12-well plate with varying concentrations of supernatant containing viral particles and 8 μg/ml of polybrene (Sigma) which was then centrifuged at 2,000 rpm for 30 minutes at 30°C.
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bioRxiv - Evolutionary Biology 2022Quote: ... A volume of 40 μL (corresponding to 4.106 cells/cm2) of suspension was plated on 6 cm plates filled with 2 mL of 2% Phytagel (Sigma-Aldrich) as previously described by Dubravcic et al ...
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bioRxiv - Cancer Biology 2022Quote: Lentivirus was titrated into 2 × 105 PC-3M cells on 6-well plates with 2 mL complete RPMI-1640 supplemented with 8 μg/mL polybrene (Sigma). After overnight infection ...
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bioRxiv - Microbiology 2022Quote: ... pneumoniae was grown at 37°C on LB agar plates supplemented with 3 μg/mL potassium tellurite (Sigma-Aldrich, USA).
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bioRxiv - Microbiology 2022Quote: ... Bacterial invasion assays were performed as described above except infection plates were incubated for 2 h before incubation with 100 μg gentamicin (Sigma) and 5 μg penicillin (Sigma ...
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bioRxiv - Neuroscience 2021Quote: EdU staining was performed on in vitro cell culture slides or on glass coverslips in 24-well plates which were precoated with poly-l-lysine (Sigma) and laminin (Thermo Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... dissociated single NSPCs were plated at a density of 5 × 104 cells per centimetre square onto tissue culture plates pre-coated with poly-D-lysine (Sigma). Cells were cultured in differentiation medium (proliferation medium minus EGF and bFGF ...
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bioRxiv - Neuroscience 2021Quote: ... and plated at a density of 1,000 cells per mm2 into 8-μwell plates (BioValley) coated with 50 μg/ml poly-D-lysine (Sigma Aldrich). Cultures were kept at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: A 5-10 µl aliquot of the filtered Aqueous Garlic Extract (AGE) was applied 1 cm away from the base of silica gel 60 TLC plate (Merck Millipore). The applied sample was air dried for 10 min at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... cut into three pieces per mouse and incubated in 24-well plates in DMEM with high glucose supplemented with 2% wt/vol FA-free BSA (FA-free BSA DMEM, Sigma). BAT was then incubated for 1 hour at 37 °C before stimulation with 10 μM isoproterenol (ISO ...
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bioRxiv - Molecular Biology 2021Quote: ... we grew cells on 60-nm plates to 70-80 % confluency and then treated them with 100 µM DRB (Sigma) for 3 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... 10-fold serially diluted and spotted onto SC-TRP plates with or without the indicated amounts of hydroxyurea (HU) (Sigma), methyl methanesufonate (MMS ...
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bioRxiv - Molecular Biology 2021Quote: ... and 90 µl of clear supernatant from each sample was transferred into each well of a 96-well black plate and proteasome activity was measured using Suc-LLVY-AMC substrate (Millipore) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... CLIP-UPF1 stable cell lines or a GFP-expressing control line were seeded in 6 × 15 cm plates and then treated with 200 ng/mL doxycycline hyclate (Sigma) for 48 hours to induce CLIP-UPF1 expression ...
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bioRxiv - Cell Biology 2022Quote: ... neurons were cultured in 24-well plates on 13 mm coverslips coated with 1 mg/mL poly-D-lysine (Sigma) and 1 µg/mL laminin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... were crossed and eggs laid overnight on day tree were collected and transferred to a new grape juice plate with Nipagin (Sigma) and the number of hatched eggs was assessed after 24 h ...
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bioRxiv - Immunology 2022Quote: Monocytic THP-1 cells were infected at a density of 2×105 cells/ml in 48 well plates in the presence of polybrene (8 μg/ml, Sigma). Infection levels were assessed at 48 h post-infection through enumeration of GFP positive cells by flow cytometry ...
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bioRxiv - Genomics 2020Quote: Twenty-four well plates were coated with: human or rat tail collagen type I (88 ng per well; Sigma-Aldrich), human collagen type III (88 ng per well ...
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bioRxiv - Genomics 2020Quote: ... were seeded in 1 ml of media in each well of a 12-well plate in the presence of 4 μg/ml polybrene (Millipore). ICR-library virus was added at increasing dilution with one well receiving no virus and serving as non-transduction control ...