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Citations for Millipore Sigma :
2901 - 2950 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... were seeded in a 200 μL total volume to white-bottom 96 well plates pre-coated with 0.01% poly-L-lysine solution (Sigma) and cultured overnight ...
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... 250,000 cells per well were seeded in 6-well plates 24 hours before adding MG- 132 (C2211, Sigma-Aldrich) at a concentration of 0.375 µM dissolved in DMSO ...
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bioRxiv - Microbiology 2024Quote: ... fresh colonies grown on blood-agar plates were incubated in Todd Hewitt Yeast Broth (Sigma-Aldrich, Saint-Louis, MO) at 37◦C until the OD600nm reached 0.7–0.9 units ...
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bioRxiv - Microbiology 2024Quote: ... enzymes on agar-solidified (1.5% w/v) TSA plates containing 0.5% (w/v) porcine gastric mucin (Type II, Sigma Aldrich). Plate assays with Tn-insertion mutants of S ...
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bioRxiv - Molecular Biology 2024Quote: 96-well flat-bottomed tissue culture plates (Earthox) were coated with varying concentrations of rat tail collagen I (Sigma) or fibronectin (Sigma ...
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bioRxiv - Biochemistry 2024Quote: Proteins were extracted from 300,000 to 400,00 HUVEC cells (per one well of a 6-well plate) with RIPA buffer (Millipore Sigma) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... at 4 x 105 cells/well on 24-well plate (Trueline) coated with 5 μg/ml fibronectin (Sigma-Aldrich). Cells were treated with either 0.01% DMSO or different concentrations of Ki8751 inhibitor for 6 hours ...
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bioRxiv - Biophysics 2019Quote: ... DBNL(1-256) and DBNL(1-374) were amplified by PCR and cloned into pET28a vector (Novagen) between BamHI and NotI sites to obtain constructs for expression of his-tagged proteins in E ...
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bioRxiv - Microbiology 2020Quote: We performed reverse transcription using the First strand cDNA synthesis kit for RT-PCR (AMV) (Sigma-Aldrich) and the protocol described by the supplier ...
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bioRxiv - Genetics 2021Quote: ... cells tested for several strains of mycoplasma contamination using LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich #D9307).
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bioRxiv - Genetics 2021Quote: ... qRT-PCR was performed using gene-specific primers (Table S3) with Kapa SyBr Fast (Sigma-Aldrich, #KK4618) in an Applied Biosystem ViiA 7 real-time PCR system.
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bioRxiv - Microbiology 2019Quote: ... the plasmid containing the insert was linearized and purified with Gene-Elute PCR Clean-Up Kit (Sigma). DNA concentration was measured with Qubit (ThermoFisher ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative PCR mixtures (10 μL) contained 5 μL Kapa SYBR FAST qPCR Master Mix (2X) (Sigma-Aldrich), 300 nM of the primer pair ...
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bioRxiv - Evolutionary Biology 2019Quote: ... cDNA was subjected to qRT-PCR using SYBR® Green JumpStart™ Taq ReadyMix™ (Sigma-Aldrich) in a CFX96 Touch™ Real-Time PCR Detection System (Biorad ...
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bioRxiv - Bioengineering 2019Quote: ... PCR was performed using control primers beyond the homology regions with KODXtreme Taq polymerase (Millipore, Massachusetts, USA). Where indicated ...
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bioRxiv - Microbiology 2020Quote: ... presence of enzyme inhibitors in RNA extracts was assessed by reverse transcription PCR (eAMV RT, Sigma-Aldrich) using universal primers for 18S RNA ...
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bioRxiv - Microbiology 2020Quote: ... at 100 V for 25 min and purified with either GenElute PCR Clean-Up Kit (Sigma Aldrich) or with Zymoclean Gel DNA Recovery Kit (Zymo Research ...
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bioRxiv - Microbiology 2020Quote: For real-time quantitative reverse transcription PCR (RT-qPCR) total cellular RNA was extracted with Trizol (Sigma) or QiAmp (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: DIG-labeled DNA probes were synthesized using Roche PCR DIG Probe Synthesis Kit (Millipore Sigma ca#11636090910). Probes were designed to target either the LexA coding sequence (Probe 1 ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines tested and confirmed to be mycoplasma free using the Mycoplasma PCR ELISA kit (Sigma). CENP-A overexpression was induced by the addition of Dox to typical growth media at 10ng/ml (considered 1X) ...
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bioRxiv - Biophysics 2020Quote: ... The resulting 5 kbp PCR fragments (Bio-DNA-DIG) (1.7 µm in length) reacted with neutravidin (Sigma) at a ratio of 100 neutravidin/DNA for 24 h at 4°C and were subsequently coupled to the anti-DIG beads at a reaction ratio of 150 neutravidin-DNA/bead for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... purified with the Illustra™ GFX™ PCR DNA and Gel Band Purification Kit (Sigma-Aldrich, USA) and sent for Sanger sequencing (Source Bioscience ...
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bioRxiv - Plant Biology 2021Quote: ... Open reading frames were PCR amplified (Phusion Hotstart II polymerase, ThermoFisher Scientific or KOD DNA polymerase, Millipore) from genomic DNA or BAC ...
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bioRxiv - Microbiology 2020Quote: ... All PCR used for cloning were performed with high fidelity KOD Hot Start DNA Polymerase (EMD Millipore). E ...
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bioRxiv - Neuroscience 2020Quote: ... DNA extraction was performed using the REDExtract-N-Amp Tissue PCR Kit (Sigma, St Louis, MO, USA).
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were PCR-amplified with 10-12 cycles using KOD Hot Start DNA polymerase (Millipore, 71086-3) and cleaned up using 1X KAPA Pure Beads ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative reverse transcription PCR reactions used the 2xKiCqStart® SYBR® Green qPCR ReadyMix™ (Sigma-Aldrich). PCR consisted of an initial activation step at 95°C for 3 min ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were purified using the kit Gene eluteTM Gel extraction kit (Sigma Aldrich, MA, USA), re-surveyed on Nanodrop® and delivered for Sanger DNA sequenced at Eurofins Genomics (Ebersberg ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were digested with NdeI and BamHI and inserted into the pET-15b plasmid (Novagen) digested with the same enzymes ...
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bioRxiv - Biochemistry 2019Quote: Genotyping of founders (F0) was done using genomic PCR with the primers following primers provided by Sigma, forward 5’-CGCGATGACAGTTTCCAGTA-3’ reverse 5’-CAAACAAAAACCCACTGAGGA-3’ ...
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bioRxiv - Molecular Biology 2019Quote: Telomerase activity was detected using the PCR-based Telomeric Repeat Amplification Protocol (TRAP) assay kit (Millipore, S7700). Briefly ...
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bioRxiv - Plant Biology 2019Quote: ... The synthesized sequences were amplified via PCR and cloned into the pET-15b expression vector (Novagen, www.merckmillipore.com) using NdeI and BamHI restriction sites to create expression constructs with an N-terminal 6-His-Tag followed by a thrombin cleavage site (pET-15b-SAP54 ...
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bioRxiv - Cancer Biology 2019Quote: ... Genotyping PCR was performed from genomic DNA from tails using standard protocol of N-Extract kit (Sigma).
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bioRxiv - Microbiology 2020Quote: Total genomic DNA was extracted using the REDExtract-N-Amp™ Tissue PCR Kit (Sigma- Aldrich, USA), which yields high quantities of DNA ...
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bioRxiv - Cancer Biology 2021Quote: ... Standard RT-PCR using independent sets of Predesigned KiCqStart® SYBR® Green Primers (Sigma, Table S11) was carried out for validation with independent biological replicates from 2D cultures (n = 2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The PCR product was subcloned between the NdeI and KpnI sites of the pCOLADuet-1 (Novagen, USA) MCS-2 using the In-Fusion HD cloning kit.
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bioRxiv - Synthetic Biology 2021Quote: ... The PCR product was subcloned between the BamHI and NotI sites of the pCOLADuet-1 (Novagen, USA) multiple cloning site (MCS)-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were routinely tested for mycoplasma contamination using the Lookout Mycoplasma PCR Detection Kit (#MP0035; Sigma-Aldrich). Cells were treated with the proteasome inhibitor MG132 (ApexBio ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were tested periodically for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit from Millipore Sigma.
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA extraction was performed with a REDExtract-N-Amp Tissue PCR Kit (Sigma, St. Louis, MO), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... PCR reactions were carried out using FastStart™ High Fidelity DNA polymerase (Sigma-Aldrich Cat no. 3553400001) in a reaction volume of 250 μl using 10 μl of the cDNA product as a template ...
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bioRxiv - Molecular Biology 2020Quote: ... An open-reading frame of the truncated Pch2 was PCR-amplified and inserted into pET15b plasmid (Novagen) in which an N-terminus of the PCH2 gene was tagged with hexa-histidine ...
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bioRxiv - Microbiology 2022Quote: Quantitative real-time PCR (rt-qPCR) was carried out using the KAPA SYBR® FAST Mix (Sigma) on a LightCycler 480 (Roche Diagnostics ...
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bioRxiv - Microbiology 2022Quote: ... the native encoding sequence was amplified by PCR and inserted into NdeI- and XhoI-digested pET36b(+) (Novagen). The fusion construct was verified by DNA sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... genotype was assessed with tail DNA extracted using RED Extract-N-Amp PCR Ready Mix (Sigma-Aldrich) followed by a polymerase chain reaction (PCR ...
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bioRxiv - Physiology 2022Quote: ... Each real-time PCR reaction mix was prepared with SYBR Green JumpStart Taq ReadyMix (Sigma, Oakville, Canada) and forward and reverse primer mix (3 µM ...
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bioRxiv - Biochemistry 2024Quote: ... The coding sequence of the DDX1 protein was PCR amplified and inserted into the pET28 vector (Novagen). A His-tag was placed in the N-terminal of DDX1 and a stop codon was inserted at the coding sequence of amino acid 708 ...
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bioRxiv - Developmental Biology 2023Quote: ... qRT-PCR was performed on Bio-Rad CFX96 real time (qPCR) system using predesigned KiCqstart primers (Sigma) and TBB green (#RR-208A ...
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bioRxiv - Immunology 2024Quote: ... Cell lines were confirmed to be mycoplasma-free with the Lookout Mycoplasma PCR Detection Kit (Sigma-Aldrich). Genotyping of various mice used in this work was accomplished with the APExBIO direct mouse genotyping kit and primers described in Table S1 ...
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bioRxiv - Genetics 2023Quote: ... we subjected the amplified PCR products to electrophoresis using gels consisting of 2-3% agarose (Sigma Aldrich) in 1 x Tris-acetate-EDTA (1 x TAE ...