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Citations for Millipore Sigma :
3051 - 3100 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed with PBS 1X three times and cell nuclei were stained with 0.5μg/mL Hoechst (Sigma Aldrich) (diluted 1:5000) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed 3 times and secondary staining was done in permeabilization buffer supplemented with 5% Donkey serum (Sigma) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... the worms were washed three times with M9 and incubated in a 10 mM serotonin solution (Sigma Aldrich, 14927) for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were washed three times in PBS and blocked with 10% Normal Goat Serum in PBS with 0.1% Triton-X (Sigma) for 1 hour at room temperature (RT) ...
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bioRxiv - Molecular Biology 2020Quote: ... The beads were washed three times with NETN buffer and then eluted with 2 mg/ml biotin (Sigma-Aldrich) for 2 hr at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were further washed three times with PBS and incubated with 5% bovine serum albumin (BSA) (Sigma-Aldrich) in PBS for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Cultures were removed and the plate was washed two times with water before adding 0.1 % crystal violet (Sigma-Aldrich) for 10 min ...
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bioRxiv - Immunology 2019Quote: ... Plates were then washed with PBS-T 3 times before the plates were incubated with 75μl Streptavidin peroxidase (1:1000, Sigma) for 60 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... Slides were washed 3 times in PBS for 5 min and incubated in DAPI (Sigma Aldrich, Cat. No.10236276001) for nuclei counterstaining ...
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bioRxiv - Microbiology 2019Quote: ... The spheroplasts were then washed 3 times in SPM buffer permeabilized by incubation in 1 mL of 0.1% triton X-100 (Sigma) in PBS for 2 minutes on ice and washed twice in PBS ...
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bioRxiv - Immunology 2020Quote: ... After incubation cells were washed 3 times with PBS containing 0.5% BSA and subsequently fixed 1% (w/v) paraformaldehyde (PFA, Merck Millipore) in PBS ...
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bioRxiv - Immunology 2019Quote: ... The plates were washed three times with PBS-T and then blocked with 280 µl/well of assay buffer [2.5% (w/v) BSA (Sigma), 2.5% (v/v ...
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bioRxiv - Immunology 2019Quote: ... Plates were then washed four times and enzyme activity detected by incubation with 100 µl/well o-phenylenediamine (Sigma) containing hydrogen peroxide for 15 min at room temperature in the dark ...
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bioRxiv - Developmental Biology 2019Quote: ... Retention time (3.8 seconds) for 5-HT were determined by a control 5-HT dilution of 1pM (Sigma-Aldrich). Results are stated as area under the curve (AUC ...
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bioRxiv - Cell Biology 2019Quote: ... Gels were then washed three times with PBS and coated with either 10 µg/ml fibronectin (Merck-Millipore, 341631) (U2OS and U-251 cells ...
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bioRxiv - Microbiology 2021Quote: ... the conjugate underwent buffer exchange three times into 1× DPBS pH 7.4 using a 4-ml Amicon Ultra centrifugal filter (30 kDa MWCO, Millipore). The conjugate was characterized using SDS-PAGE ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... The samples were washed with PBS three more times and incubated with 1.0 mg/mL 4,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich) for nuclear staining ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were released via 0.12% trypsin without EDTA and washed 3 times with PBS (Sigma-Aldrich, St. Louis, MO). 0.4% trypan blue (Thermofisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: Cryo-sections were let to stabilize at room temperature for at least 2 hours and then washed three times in PBST (1X PBS with 0.1% Triton X-100, Sigma). To block endogenous peroxidase ...
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bioRxiv - Neuroscience 2021Quote: ... The PVDF membrane was rinsed three times with a 0.1 M Tris-buffered saline (TBS) solution containing 0.5% Tween-20 (TBS-Tween; Sigma-Aldrich) and blocked in TBS-Tween with 5% (w/v ...
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bioRxiv - Biophysics 2020Quote: ... the culture medium was removed and cells were rinsed 3 times with Dulbecco’s Phosphate Buffered Saline (DPBS, Sigma-Aldrich).
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bioRxiv - Neuroscience 2019Quote: ... After 3 times washing with PBS-T [PBS with 0.5% (v/v) Triton X-100 (Sigma Aldrich, # X-100)] fixed brains were incubated with primary antibody containing 10% Normal Donkey Serum in 0.4% PBS-T overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cells were then rinsed 3 times with PBS followed by permeabilization with 0.1% triton X-100 (Sigma, X100) in PBS for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... the conjugate underwent buffer exchange three times into 1x DPBS pH 7.4 using a 4-ml Amicon Ultra centrifugal filter (30 kDa MWCO, Millipore). The conjugate was characterized using SDS-PAGE ...
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bioRxiv - Microbiology 2020Quote: ... For all ChIP experiments a mock IP and an experimental IP were set up at the same time using 1,350 µl pre-cleared chromatin and 10 µg of normal mouse IgG (Millipore) and monoclonal mouse anti-c-Myc respectively ...
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bioRxiv - Systems Biology 2021Quote: ... The retention times of the eluted peaks were compared to those of commercial analytical standards (Sigma-Aldrich, Madrid, Spain). Metabolite concentrations were quantified by the calibration graphs (R2 value > 0.99 ...
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bioRxiv - Neuroscience 2021Quote: ... glucose solution at 37 °C for 20 min and rinsed five times with washing buffer (PBS −/−with 0.2% fish skin gelatin (G7765, Sigma)) ...
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bioRxiv - Microbiology 2020Quote: ... Metabolite peaks were identified by their exact mass and matching retention times to those of pure standards (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... The plates were washed five times with TBS containing 0.05% v/v Tween-20 and polyclonal anti-human antibody conjugated to HRP (Sigma) added for one hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Plates were then washed 3 times with PBST before the addition of 100ul of .5mg/ml o-Phenylenediamine (Sigma) diluted in phosphate-citrate buffer with sodium perborate (Sigma) ...
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bioRxiv - Genetics 2020Quote: ... Islets were dissociated from the exocrine tissues by shaking vigorously several times followed by Histopaque 1077 (Sigma-Aldrich, 10771) density centrifugation (900g ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then washed three times with distilled water and spun down in low melting agarose 2 % (Sigma Aldrich) and solidified in ice ...
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bioRxiv - Microbiology 2019Quote: ... The antigen bound Magnetic Beads were washed five times with the lysis buffer and eluted by 2.5 volume of 3×FLAG peptide (150 ng/µl, Sigma) for 3h at 4°C with gently shaking ...
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bioRxiv - Microbiology 2019Quote: ... Larvae were washed three times in 0.05% PBS-Tween20 before incubation in 10 µg/ml Hoechst (#B2883, Sigma-Aldrich) diluted in Tris-buffered saline ...
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bioRxiv - Biophysics 2021Quote: ... the flow cell was flushed three times with 20 μL of 1 mg/mL bovine serum albumin (EMD Millipore) and incubated for 5 min to block the surface from nonspecific interactions ...
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bioRxiv - Cell Biology 2021Quote: ... twice in Tris-HCl (pH 8.5) and three times in 100 mM triethylammonium bicarbonate buffer (TEAB) pH 7.5 (Sigma-Aldrich). The washed beads were resuspended in 200 μL 50 mM ammonium bicarbonate (pH 8.3 ...
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bioRxiv - Cell Biology 2021Quote: ... coverslips were washed three times in PBS-gly and anti-GFP was further labelled with ATTO647N (1:500, Sigma) and anti-mCherry was labelled with CF568 (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Cryo-sections and tail skin whole mounts were washed three times in PBS and fixed in 4% Paraformaldehyde (Sigma) in PBS for 30 mints at RT ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed 3 times in PBS and blocked in PBS supplemented with 0.5% fish skin gelatin (Sigma-Aldrich) for 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed 3 times in staining buffer (PBS with 0.1% Bovine Serum Albumin (cat. num. A8806, Sigma), 0.05% NaN3 (cat ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed five times and stained with 4',6-Diamidino-2-phenylindole (DAPI) (Sigma, Cat No. 10236276001, Germany) for 20 min ...
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bioRxiv - Microbiology 2021Quote: ... plates were washed three times with phosphate buffered saline (PBS-Tween 20 [pH=7.4]; catalogue number 524653, Millipore Sigma), blocked with 200 μL per well casein blocking buffer (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were finally washed 3 times in PBS-Tween 20 0.1% and mounted in Mowiol 4-88 (81381, Sigma).
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bioRxiv - Systems Biology 2022Quote: ... Formic acid-extracts were spun down at 13k rpm for 10 minutes and a total of 2 μl supernatant was spotted 0.5 μl at a time near the base of a PEI-Cellulose F thin layer chromatography plate (Millipore). The spots were dried and nucleotides were resolved using freshly prepared 1.5M KH2PO4 (pH 3.4) ...
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bioRxiv - Microbiology 2022Quote: ... Nexcelom Biosciences) at the time of harvest by staining with a 0.2% final concentration of trypan blue (Sigma; T8154) in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein pellet was washed 3 times in a solution of 0.3 M guanidine hydrochloride (Sigma, Cat. No. SRE0066) in 95% ethanol ...
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bioRxiv - Neuroscience 2019Quote: ... Thereafter the sections were rinsed three times in 0.1 M sodium cacodylate buffer (pH 7.2-7.4) (Sigma-Aldrich, Germany) and incubated with 1 % osmium tetroxide (Science Services ...
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bioRxiv - Neuroscience 2021Quote: ... washed 3 times for 5 min with PBS and permeabilized for 1h at room temperature (permeabilization buffer: 2,5% donkey serum (Sigma), 1% BSA ...
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bioRxiv - Immunology 2020Quote: ... the sections were washed 3 times in PBS and permeabilized in PBS containing 0.3% Triton-X100 (#X100, Sigma-Aldrich) for 2 hours ...
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bioRxiv - Immunology 2021Quote: ... with the exception that the samples were spiked with isotope-labelled peptides and MSRT retention time peptides standards (Sigma), prior to MS analysis.