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Citations for Millipore Sigma :
2951 - 3000 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured after 72hrs (or at the indicated time point) using the MTT assay (Sigma M2128), with 500ug/mL salt diluted in complete medium and incubated at 37°C for 3 hrs ...
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bioRxiv - Cancer Biology 2023Quote: Tumors were collected at different time points and enzymatically digested in 10mg.mL−1 collagenase and 0.2mg.mL−1 DNAse I (Sigma-Aldrich) for 30min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Then the wells were washed 2 times with PBS and 200 μl of SLC3A2 (1: 500) (Sigma / HPA017980) primary antibody solution (in 5%BSA ...
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bioRxiv - Neuroscience 2023Quote: ... the slices were washed three times in PBS and incubated in DAPI solution (MBD0015, Sigma-Aldrich, Missouri, USA) to label cell nuclei ...
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bioRxiv - Cell Biology 2023Quote: ... The reaction was quenched by washing three times with the quenching buffer (containing 5 mM Trolox [Sigma-Aldrich, cat#238813-25G] ...
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bioRxiv - Microbiology 2023Quote: ... and the cells were washed 3 times in PBS before addition of 2% Triton X– 100 (Sigma-Aldrich) PBS solution to lyse the cells for enumeration of the intracellular bacteria ...
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bioRxiv - Bioengineering 2023Quote: ... Diabetes was induced through a one-time intraperitoneal injection of 110 mg kg−1 streptozotocin (Step, Sigma Aldrich) dissolved in citrate buffer (pH 4.5 ...
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bioRxiv - Neuroscience 2023Quote: ... and retention time comparison against an in-house standard library (Sigma-Aldrich, IROA Technologies, Sea Girt, NJ, USA). Pathway analysis and analysis of metabolite set enrichment was performed using MetaboAnalyst (Xia and Wishart ...
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bioRxiv - Neuroscience 2023Quote: ... The mixtures were washed three times with 1X PBS with 0.15% Triton X-100 (Sigma, Cat#X100-500ML), and then resuspended in 20 μl of 1X NuPAGE LDS sample buffer for NuPAGE and immunoblotted analysis ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were then washed three times with IP lysis buffer and eluted using 50 mM DTT (Sigma-Aldrich) and 0.5% SDS in lysis buffer at 58°C for 30 min ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were rinsed with 1x PBS three times and incubated with blocking buffer (0.5% BSA (Sigma-Aldrich A7030) in 1x PBS ...
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bioRxiv - Cell Biology 2024Quote: ... coloration was differentiated by dipping 10 times in two different baths of acetic acid 0.5% (Sigma-Aldrich – 33209). Imaging was performed using a Nikon Eclipse Ci-S optical microscope.
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bioRxiv - Molecular Biology 2024Quote: ... At the same time 100 mio cells / library were plated into 0.1 mM ABA (Sigma-Aldrich, 90769-25MG). After 48 h treatment with ABA ...
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bioRxiv - Microbiology 2024Quote: ... They were washed three times with PBS and blocked with 3% bovine serum albumin (BSA) (Sigma-Aldrich, A3294) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed and fixed to the coverslips at the indicated times with 10% neutral buffered formalin (Sigma). Coverslips were permeabilized ...
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bioRxiv - Cell Biology 2022Quote: ... CRISPR/Cas9-edited lines were subjected to staining with the Duolink™ In Situ Detection Reagents Orange (DUO92007) and the combination of α-mouse and α-rabbit probes (DUO92002 & DUO92004) from Sigma-Aldrich, as per manufacturers’ recommendations ...
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bioRxiv - Neuroscience 2019Quote: ... PLA was performed using PLA PLUS or MINUS probes for rabbit or mouse anti-serum and Duolink In Situ Detection Reagents Red (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were then washed six times with TNT for at least 20 min each and then subjected to second-round probe detection by either anti-DIG-POD Fab fragments (Sigma-Aldrich; Cat. No. 11207733910) or Anti-Fluorescein-POD Fab fragments (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... Loaded samples (2.03 μL) were determined to be within the linear range of detection of the MAGPIX (MAGPIX-XPON4.1-CEIVD; EMD Millipore Corporation, Burlington, MA, USA) system ...
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bioRxiv - Cancer Biology 2022Quote: ... cell death detection enzyme-linked immunosorbent assay PLUS (ELISA) and human anti-β-actin antibody were obtained from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Poly-ADP-ribose polymerase (PARP ...
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bioRxiv - Genomics 2021Quote: ... Cells were permeabilized by 0.1% Triton X-100 for 5min at RT and stained with antibody against the mitosis specific antigen pMPM2 (Millipore, 05-368, 0.2µl/10million cells) for 50min at RT ...
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bioRxiv - Microbiology 2020Quote: ... Following a 15 min entry phase under standard cell culture conditions the cells were fixed and the nuclei stained for 1 h at RT by addition of 33 μl 16% PFA and 4 μg / ml Hoechst 33342 (Sigma-Aldrich, St. Louis, USA) in PBS ...
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bioRxiv - Microbiology 2020Quote: ... the cells were fixed and the nuclei stained for 1 h at RT by addition of 33 μl 16% (w / v) para-formaldehyde (PFA)and 4 μg / ml Hoechst 33342 (Sigma-Aldrich, St. Louis, USA) in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... or cellulose solution in N-hydroxysuccinimide (NHS; Aldrich; 100 mM) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma; 100 mM, 90 min, RT)44 ...
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bioRxiv - Molecular Biology 2020Quote: Cells were washed three times with saline at 200 × g for 10 min at RT to remove residual Percoll and suspended in RPMI 1640 medium (Cellgro, Manassas, VA) supplemented with 5% human serum (Sigma-Aldrich, St. Louis, MO), unless otherwise stated ...
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bioRxiv - Genomics 2019Quote: ... partheno-sporophyte material was chemically cross-linked for one hour at RT using formaldehyde (final concentration: 3% in 1X PBS; final volume: 30 ml; Sigma-Aldrich, St. Louis, MS). The formaldehyde was then quenched for 20 min at RT by adding 10 ml of 2.5 M glycine ...
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bioRxiv - Biochemistry 2021Quote: ... and lymph node from BALB/cJ mice and humans were harvested and either fixed for 48 h at RT in 10% neutral buffered formalin (Sigma-Aldrich, St. Louis, MO) or zinc-fixation buffer (pH 6.5 - 7) ...
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bioRxiv - Physiology 2020Quote: ... Cells were incubated at RT for 3 hrs in 300 μl secondary antibody solution containing anti-rabbit TRITC (Sigma-Aldrich Cat# T6778, RRID: AB_261740) at a concentration of 1:75 in 1× TBS ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were incubated for 24 hours at RT on a rocking platform with ATTO647N-conjugated anti-rabbit antibodies (Sigma-Aldrich, catalog no. 40839) diluted to 1:250 in the antibody dilution buffer ...
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bioRxiv - Neuroscience 2022Quote: ... secondary antibodies were applied for 30 min at RT at 1:400 dilution in blocking solution containing 1:10,000 4’,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich, St. Louise, MO, USA) in the closed humid chamber ...
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bioRxiv - Genomics 2022Quote: ... slides were incubated in 1% Kodak PhotoFlo at RT for 1 minute and counterstained with 0.5 mg/mL DAPI (4′,6-diamidino-2-phenylindole, Sigma-Aldrich, St. Louis, MO, USA) in antifade based on DABCO (1,4-diazabicyclo(2.2.2)-octane ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNAs from tomato samples were isolated and fractioned to >200 nt and <200 nt populations using the RNAzol RT reagent (Sigma-Aldrich, St. Louis, MO). sRNA species were further purified using the miRVana miRNA isolation kit (Thermo Fishier Scientific ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... the cells were incubated for 1 h in the dark and at RT with 30 μL drops containing secondary antibody and DAPI (Sigma, 10 236 276 001) diluted 1:1000 in blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... the coverglasses were coated for 2 h at room temperature (RT) with a 20 μg/ml solution of poly-D-lysine (PDL; Sigma–Aldrich, cat. no. P6407) in sterile double distilled water (ddH2O) ...
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bioRxiv - Biophysics 2021Quote: ... 2.5 nM divalent streptavidin were incubated for 10 min (RT) prior to the addition of 100 nM biotin labeled bovine albumin (#A8549, Sigma Aldrich, St. Louis, USA), biotinylated Pierce™ protein A (#29989 ...
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bioRxiv - Developmental Biology 2022Quote: ... The cell nuclei were stained with 4,6-diamidine-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich; 50 mg/ml in PBS for 15 min at RT). Immunostained cells analyzed with confocal microscopy ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were resuspended in 50mM ammonium bicarbonate pH 8.0 and reduced for 30 min at room temperature (RT) with 5 mM TCEP and then alkylated at 50 mM iodoacetamide (I114 - Sigma, St Louis, Missouri, USA) for 30 min ...
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bioRxiv - Bioengineering 2024Quote: ... for 1-3 hours at room temperature (RT) and then permeabilized with 0.1% (v/v) Triton X-100 (Sigma-Aldrich, Cat # 9036-19-5) for 1 hour before blocking with 3% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed thrice using PBS and blocked and permeabilized using saponin-BSA buffer for 1 h at RT (0.2% saponin from Millipore and 1% BSA from Fisher). Cells were incubated with the following primary antibodies for 1 h at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then stained with a 1X crystal violet solution for 20 minutes at RT (2% crystal violet, Sigma; 20% ethanol; 4% formaldehyde, Merck). Plates were washed with clear water and plaques were counted manually ...
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bioRxiv - Immunology 2023Quote: ... The resulting single-cell suspension was then pelleted at 400g for 10 minutes at 4°C and resuspended in 25mL RT 30% Percoll (Millipore-Sigma GE17-0891-01) in 1X HBSS without calcium or magnesium (Gibco 14185-052) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed twice with PBS at room temperature (RT) and lysed in 1x (radio immunoprecipitation assay buffer (RIPA buffer, Merck Millipore, cat. no. 20-188) containing 0.1% SDS ...
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bioRxiv - Physiology 2024Quote: ... Tissues/organs were then permeabilized by incubating on a rocker for 1 h at RT in 4% Triton X-100 (Sigma Aldrich, Oakville, ON, Canada), 10% normal sheep serum (NSS ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were blocked with 5% BSA in 1X PBS containing 0.1% Tween-20 (PBT) at RT for 1 hour and then incubated with primary antibody solution containing anti-chondroitin sulfate antibody (Millipore, monoclonal, mouse, 1:100) and biotinylated-Hyaluronan Binding Protein (Millipore ...
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bioRxiv - Immunology 2024Quote: ... Cells were fixed for 30min in 4% paraformaldehyde (PFA) at RT and nuclei were stained with 1µg/mL propidium iodide (IP) (Sigma-Aldrich, St. Louis, MO, USA). Cells were washed with PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... The lactate assay kit (Lactate Assay kit, Sigma Aldrich) was used to measure L (+)-Lactate in the culture media according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR amplifications were performed using KOD Hot Start DNA polymerase (Merck Millipore). All restriction enzymes ...
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bioRxiv - Molecular Biology 2019Quote: ... Library PCRs were supplemented with 2x SYBR dye (Sigma – catalogue no. S9430) so that amplification could be monitored by quantitative PCR on a Roche lightcycler 480 ...
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bioRxiv - Molecular Biology 2019Quote: ... Library PCRs were supplemented with 2x SYBR dye (Sigma – catalogue no. S9430) so that amplification could be monitored by quantitative PCR on a Roche lightcycler 480 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... For the PCRs we used a standard Taq DNA polymerase (Sigma Aldrich) and limited the elongation time to 90 seconds so that an homozygous insertion of the 5-kb ONSEN TE or the T-DNA would prevent the formation of a PCR-product ...