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Citations for Millipore Sigma :
3301 - 3350 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... slices were washed three times in cold dissociation solution and then transferred to an enzyme inhibitor mix containing trypsin inhibitor (10 mg/mL, Sigma), bovine serum albumin (BSA ...
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bioRxiv - Immunology 2021Quote: ... The beads were then washed with IP buffer three times and incubated in FLAG-elution buffer (100 μg/mL FLAG peptides (Sigma), 50 mM Tris-HCl pH7.4 ...
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bioRxiv - Microbiology 2019Quote: ... During dissection the sections were washed three times with a 1:1 mix of RPMI 1640 and Dulbecco’s modified Eagle medium (DMEM) (Sigma-Aldrich), supplemented with 50 μg ml-1 ampicillin ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were then washed three times with PBS and then lysed in PBS/ 1% NP40 + complete protease inhibitor cocktail (Sigma) and phosphatase inhibitor cocktail 2 (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: ... washed 3 times in PBS and then incubated for 30 min with secondary antibodies and DAPI (Sigma, 1:1000 dilution); all antibodies were diluted in PBS + 3% BSA and are as follows ...
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bioRxiv - Bioengineering 2019Quote: Cy3-(GT)6-SWCNTs and Cy3-(GT)30-SWCNTs were first filtered 3 times using 100kDa Amicon centrifuge filters (Millipore) to remove free Cy3-DNA from solution ...
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bioRxiv - Bioengineering 2019Quote: ... they were washed again 2 times with the wash buffer and incubated with 5% v/v goat serum (Sigma Aldrich) for 1 h ...
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bioRxiv - Developmental Biology 2019Quote: ... Fixed whole-mount tissues were washed 3 times with PBS-Triton (0.1%) and blocked with 20% goat serum (Sigma G6767) for 1 hr ...
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bioRxiv - Neuroscience 2019Quote: ... just prior to imaging (time 0 hr). Unless specified, FK866 (kind gift of Prof. Armando Genazzani, University of Novara) and NMN (Sigma) were added at the same time as CCCP ...
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bioRxiv - Genetics 2019Quote: ... cells were immobilized onto glass bottom wells and washed three times with liquid EMM2 containing Hoechst 33342 (Sigma-Aldrich B2261) at 1 μg/ml plus Torin1 (20 μM) ...
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bioRxiv - Cell Biology 2019Quote: Cells were washed with PBS then incubated with CSK buffer 3 times for 3 minutes and once for half minute [10 mM Hepes pH 7.0 (Sigma-Aldrich), 100 mM sucrose (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2019Quote: ... Time points over the course of 120 min were quenched into 500 mM KOH and analyzed by electrophoretic TLC (Millipore). TLC plates were equilibrated with pyridine acetate buffer (5 mL pyridine ...
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bioRxiv - Genetics 2019Quote: ... Cells were collected at different time-points of phase two growth to monitor erythroid differentiation and extract RNA using Tri-reagent (Sigma). Differentiation was assessed using qRT-PCR (with Taqman probes for HBB and HBG2 ...
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bioRxiv - Biophysics 2019Quote: ... (4) four times BRB40 buffer (40 mM Pipes [Sigma], pH 6.84, with KOH [VWR], 1mM EGTA [Sigma], 4mM MgCl2 [VWR]) to remove excess F-127 in solution and exchange buffers ...
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bioRxiv - Microbiology 2019Quote: ... This step was repeated one more time and the pellet was resuspended in 2 mL of GAD reagent (1 g glutamic acid (Sigma), 3 mL triton X-100 (Winkler) ...
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bioRxiv - Immunology 2020Quote: ... Beads were eluted three times at room temperature for 15 min each using Influenza Hemagglutinin (HA) peptide (Sigma Aldrich I2149).
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bioRxiv - Microbiology 2019Quote: ... The membranes were finally washed again 4 times for 10 min in TBS and revealed with Immobilon Western Blotting Chemoluminescence HRP substrate (Merck Millipore) and Super RX-film (Fujifilm ...
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bioRxiv - Cancer Biology 2019Quote: ... 6 week-old mice were injected intraperitoneally three times at one day interval with tamoxifen (T5648; Sigma-Aldrich, Bornem, Belgium) dissolved in corn oil at a concentration of 30 mg/mL and at a dose of 150 mg/kg of body weight ...
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bioRxiv - Immunology 2021Quote: ... biopsies of central tumour and normal mucosal tissue were removed and washed intact 5 times with Hank’s balanced salt solution (HBSS) supplemented with 10% penicillin/streptomycin (both Sigma-Aldrich). After washing ...
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bioRxiv - Biochemistry 2020Quote: ... Bound protein was eluted seven times with 0.5 column volumes of buffer A with 3 mM D-Desthiobiotin (Sigma-Aldrich), 0.026% DDM and 0.1 mM AEBSF protease inhibitor ...
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bioRxiv - Biophysics 2021Quote: Transfected and infected cells were fixed at the indicated time points with 4 % (w/v) paraformaldeyde (Sigma Aldrich, Taufkirchen, Germany) in DPBS+/+ ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were rinsed with Milli-Q H2O three times and coated with laminin (20 μg/ml in ultra-pure H2O, 11243217001, Sigma) for 1 hr at 37°C ...
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bioRxiv - Neuroscience 2021Quote: Coronal sections were rinsed and permeabilized three times in 1xPBS with Triton X-100 at 0.5% (Sigma-Aldrich, Madrid, Spain) for 10 minutes each ...
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bioRxiv - Cancer Biology 2020Quote: Cells exposed to either DMSO or TMZ for the desired amount of time were washed twice with sterile PBS and then exposed to 1% PFA (Sigma) for crosslinking ...
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bioRxiv - Cell Biology 2019Quote: ... After the activation gels were washed three times with PBS and coated with either fibronectin (Merck-Millipore, catalog number 341631) (20 μg/ml ...
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bioRxiv - Plant Biology 2021Quote: ... supernatants were discarded and the beads were washed 5 times using extraction buffer containing 0.5 % (v/v) IGEPAL CA-630 (Sigma-Aldrich). To release the proteins ...
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bioRxiv - Biochemistry 2021Quote: ... elution was performed three times each using 20 μL of a 150 μg/μL stock of 3xFLAG peptides (Sigma Aldrich). Western blot analysis was done as previously described [7] ...
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bioRxiv - Neuroscience 2020Quote: ... The mixture was centrifuged at 400 × g for 5 min and concentrated three times with a Centriprep centrifugal filter (Cat. 4310, Millipore) at 1,220 × g (20 min each ...
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bioRxiv - Plant Biology 2020Quote: ... The precipitations were washed four times with ice-cold immunoprecipitation buffer at 4°C and were analyzed by immunoblot using anti-Myc (Sigma), or anti-GFP (Cell Signaling Technology ...
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bioRxiv - Systems Biology 2020Quote: ... At time 0 medium was removed from cells and recombinant mouse tumor necrosis factor alpha (TNF-α, Sigma-Aldrich, #T7539) was added at concentrations 0-100 ng/mL as 5-minute pulse ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed two times and horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (Sigma Aldrich, St. Louis, MO) was used at a 1:5,000 dilution and incubated at room temperature for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed multiple times in TBS-T for 1h before adding secondary antibody (goat anti-mouse alkaline phosphatase conjugate; Sigma) as above at 1:2500 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... The plates were later washed with sterile distilled water 3 times and incubated with 250 μl of 50 μg/ml fibronectin (Sigma) overnight at 4°C until further neuronal culturing.
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bioRxiv - Microbiology 2020Quote: ... Adjusted cultures were 1:10 serially diluted 5-times and 5 µl of each dilution spotted on YPD agar plates containing indicated concentrations of caspofungin (Sigma). Plates were incubated at 30°C for 3 days and photographed ...
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bioRxiv - Microbiology 2019Quote: ... The cells were washed 3 times with PBS containing 0.1% Tween-20 (Sigma Aldrich, Castle Hill, NSW, Australia; PBS-T) before being stained with 400μl AlexaFluor 488-labelled anti-Rabbit IgG (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Percentage of GFP positive cell population was measured at day 3 or day 4 as initial time point using a Guava Easycyte HT instrument (Millipore). GFP% (mCherry% ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were washed 3x in TBST (total wash time 1 hr) and were developed with luminata crescendo ECL substrate (Millipore) on an ImageQuant LAS 4000 (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... these were washed three times with PBS and incubated for 2h with the anti-mouse IgG (whole molecule)-FITC secondary antibody (Sigma). Myonemes ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed three times with HBSS buffer containing 20 mM HEPES and 0.1 % fatty acid-free bovine serum albumin (BSA) (Sigma-Aldrich). Cells were then placed into the Epic-DMR-reader and equilibrated for 1 hour at 37 °C to achieve baseline stabilisation ...
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bioRxiv - Biochemistry 2022Quote: ... The purified RNA was buffer exchanged three times into RNase-free water using 30 kDa cutoff Amicon centrifugal filters (Millipore). The RNA was stored at -20°C until preparation of cryo-EM grids.
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bioRxiv - Microbiology 2022Quote: ... At the required time the cells were washed with PBS and fixed with 4% paraformaldehyde (Sigma-Aldrich, Burlington, Massacchusetts, USA) in PBS for 30min prior to further processing.
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bioRxiv - Plant Biology 2022Quote: ... Seedlings were then washed 2 times with PBS and cell walls were stained with 0.1% Calco fluor white (Fluorescent brightener 28 F3543-1G Sigma-Aldrich) in PBS for 30 min in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated at 30°C and at the indicated times 2 μL aliquots were spotted on TLC PEI Cellulose F (Millipore) to stop the reaction ...
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bioRxiv - Microbiology 2022Quote: ... and buffer exchanged three times into protease buffer (20 mM Tris pH 8.0, 10% glycerol) using an Amicon Ultra centrifugal filter (Sigma-Aldrich). Purified His6-tagged TEV protease was added to all proteins at a 1:20 ratio (except for SpoVD ...
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bioRxiv - Microbiology 2022Quote: ... These wells were washed five times using 1× PBST and followed by addition 50 μl/well of anti-human IgG horseradish peroxidase (HRP) (Sigma) diluted in Stabilzyme Noble (Surmodics) ...
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bioRxiv - Plant Biology 2022Quote: ... The time channel in the GC-MS spectrum for IAA was identified using a commercial IAA powder (from Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... cell de-adhesion dynamics was quantified by performing live cell time lapse imaging following treatment with 0.5mM EDTA (Sigma-Aldrich). For the experiment ...
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bioRxiv - Immunology 2022Quote: ... and washed four times in citrate-PBS supplemented with 0.01% bovine serum albumin (BSA, 10735086001, Sigma-Aldrich, Zwijndrecht, The Netherlands). PBMCs were frozen at 105 cells/ml in freezing medium containing RPMI-1640 (RPMI ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed three times in PBS and blocked in PBS supplemented with 0.5% fish skin gelatin (G7765, Sigma-Aldrich) for 20 min ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed 3 times by adding and removing 300 μl of wash buffer to each well (PBS + 0.05%Tween-20; Sigma, UK) and incubated for 1 hour with blocking buffer (Reagent Diluent ...