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Citations for Millipore Sigma :
2851 - 2900 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... washed 3 times with phosphate-buffered saline (PBS) and permeabilized in 0.4% Triton X-100 (T878, Sigma-Aldrich), 1% donkey serum (D9663 ...
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bioRxiv - Immunology 2019Quote: ... Sections were then washed for three times with wash buffer before mounting in Fluoromount Aqueous Mounting Medium (Sigma). WGA-488 and tdTomato were visualized directly based on endogenous fluorescence ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... 0.1% SDS), two times with LiCl wash buffer (10mM Tris-HCl pH 8.0, 1mM EDTA, 250mM LiCl (Sigma), 0.5% NaDeoxycholate ...
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bioRxiv - Systems Biology 2019Quote: Yeast cells in log-phase growth (OD = 0.5) were concentrated 10 times (OD = 5) by a glass filter system with a 0.45 μm filter (Millipore). Cells were exposed to a final osmolyte concentration of either 0.2 M or 0.4 M NaCl ...
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bioRxiv - Bioengineering 2021Quote: ... then rinsed 3 times with PBS and permeabilized with staining buffer (0.1% Triton X-100/PBS, Sigma, 93443) for 1 hour before immunolabeling.
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed three times with PBS and mounted in fluorescence mounting medium (Mowiol 4-88, Sigma Aldrich). Immunofluorescence microscopy was performed using the confocal microscopes LSM 780 and LSM 800 Airyscan (both from Carl Zeiss ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were rinsed 3 times in 1X PBS and permeabilized with 0.1% Triton X-100/PBS (Sigma, 93443) for 15 min at RT ...
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bioRxiv - Bioengineering 2020Quote: ... Patchoulol was identified by comparison of retention time and mass spectra with a primary reference standard (Sigma-Aldrich) and was quantified using a standard curve (R2 > 0.99).
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bioRxiv - Biochemistry 2020Quote: ... Membranes were quickly washed 3 times with TBS/T (0.1% Tween20, Sigma #P5927; 20mMTris-HCL pH 7.6, T7943, Sigma, 150mM NaCl, Sigma) then stained with Ponceau S (PoncS ...
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bioRxiv - Neuroscience 2020Quote: ... Time pregnant female C57/Bl6 mice were injected with either saline or VPA (Sigma-Aldrich, 600mg/kg, subcutaneous) on E12.5 ...
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bioRxiv - Physiology 2020Quote: ... Cells were treated at dose and time indicated with palmitate or vehicle (BSA) using sodium palmitate (Sigma Aldrich) conjugated to BSA in ethanol at 65°C ...
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bioRxiv - Neuroscience 2021Quote: ... washed two times within PBS and PLA was realized according to the instructions of the manufacturer (DuoLink, Sigma). Primary antibodies used were as shown in Supplementary Table S4 ...
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bioRxiv - Neuroscience 2021Quote: ... the slices were washed with PBS three times and then permeabilized in 0.1% Triton X-100 (Sigma-Aldrich) for 15 min at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... The worms were washed 2–3 times with PBS-T and then stained with 0.125% thioflavin-S (Sigma) in 50% ethanol for 2 min and then de-stained sequentially in ethanol (50% ...
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Plant pathogens convergently evolved to counteract redundant nodes of an NLR immune receptor networkbioRxiv - Plant Biology 2021Quote: ... Beads were washed five times with immunoprecipitation wash buffer [GTEN extraction buffer with 0.3% (v/v) IGEPAL (SIGMA)] and re-suspended in 70μl SDS loading dye ...
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bioRxiv - Microbiology 2021Quote: ... The beads were washed with 1x Tris-buffered saline (TBS) three times and proteins were eluted with 55μL of 100μg/mL 3xFLAG peptide (Sigma) in 1x TBS ...
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bioRxiv - Microbiology 2021Quote: ... Wells were washed a final time and the assay was developed using the SigmaFast pNpp reagent (Sigma Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... tissues were washed 3 times in PBS and stored at 4°C in PBS containing 0.05% NaN3 (Sigma) until use ...
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bioRxiv - Microbiology 2021Quote: ... Concentrations were determined using external standards by comparing the retention time (all compounds were purchased from Sigma-Aldrich). Peaks were integrated using the EZChromElite software (Version V3.3.2.SP2 ...
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bioRxiv - Microbiology 2021Quote: ... The resulting liquid was aliquoted into 4 phase lock tubes (Quanta Biosciences) and extracted 3 times with an equal volume of phenol:chloroform:isoamylalcohol (25:24:1, Sigma). A final extraction with an equal volume of chloroform (Sigma ...
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bioRxiv - Pathology 2021Quote: Vibratome sections were washed three times with phosphate-buffered saline (PBS) containing 0.4% Triton X-100 (Sigma-Aldrich) (0.4% PBTx) ...
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bioRxiv - Cancer Biology 2020Quote: ... and HLA complexes subsequently eluted three times with 200 mL 1% trifluoracetic acid (TFA, Sigma/Aldrich, cat. # 02031). For the separation of HLA ligands from their HLA complexes ...
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bioRxiv - Developmental Biology 2020Quote: ... cells are washed 3 times with PBS and blocking solution is added (Bovine Serum Albumin, BSA. Sigma; A7906) at 3% in PBS for at least 30 minutes ...
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bioRxiv - Biophysics 2022Quote: ... The beads were washed three times with filtered PBS and incubated with nitrogen vacancy fluorescent nanodiamonds (798134, Sigma) in a 1:1 ratio for 10 minutes at room temperature ...
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The Batten disease protein CLN3 is important for stress granules dynamics and translational activitybioRxiv - Molecular Biology 2022Quote: ... Coverslips were then incubated with 200μl of primary antibody solution for 1 h at RT and washed 3 times with PBS before incubation with secondary antibody solution containing 0,1μg/mL DAPI solution (Sigma) for 1 h at RT ...
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bioRxiv - Biophysics 2022Quote: ... The fixed neurons were rinsed three times with PBS and permeabilized in 0.2% Triton X-100 (Millipore Sigma) in PBS for 5min ...
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bioRxiv - Cancer Biology 2022Quote: ... Mice were given 6 times weekly with a dose of 1.0 mg DMBA (Sigma-Aldrich, Milwaukee, WI, USA) in 0.2 ml of corn oil or an equal volume of corn oil alone (vehicle ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were diluted 10 times in 20 mM TCEP in 20 mM triethylammonium bicarbonate (TEAB, Sigma-Aldrich) buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were centrifuged a second time and pellets resuspended in 1 mL 0.25 % Triton X-100 (Sigma Aldrich) in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... and dipped three times in the 25% w/v mixture of Bovine Serum Albumin (BSA) (B4287, Sigma Aldrich)) in the cell medium ...
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bioRxiv - Immunology 2021Quote: ... Subsequently cells were washed 3 times with PBS before being treated with 2% saponin (250 µL/well) (Sigma) in distilled water and incubated for 15 min at 37°C and 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the planarians were washed three times with IO water and homogenized in 1% Triton X-100 (Sigma-Aldrich) in PBS as previously described (Hagstrom et al ...
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bioRxiv - Cancer Biology 2022Quote: ... The membranes were washed again with TBST three times and visualized with chemiluminescence using HRP substrate (Millipore, #WBKLS0500) or Pico (Thermo Fisher ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were washed three times with 1x PBST and incubated in 1 mg/mL DAPI (Sigma-Aldrich D9542) diluted at 1:500 in 1x PBS at room temperature for 1 hour ...
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bioRxiv - Developmental Biology 2022Quote: ... The fixed cells were then washed three times with PBS and permeabilized with 0.3% Triton-X100 (Sigma-Aldrich) diluted in PBS for 15 min at rt ...
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bioRxiv - Neuroscience 2019Quote: ... After 3 times washing with PBS-T [PBS with 0.5% (v/v) Triton X-100 (Sigma Aldrich, # X100)] fixed brains were incubated with primary antibody containing 10% Normal Donkey Serum in 0.4% PBS-T overnight at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... The brains were then washed three times with PBS containing 0.5% Triton X-100 (PBST, Sigma-Aldrich, T8787); each time ...
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bioRxiv - Plant Biology 2019Quote: ... Identification was performed based on retention times and masses of salicylic acid and 4-chlorobenzoic acid standards (Sigma). The amount of SA was calculated on the basis of its relative response factor to the internal standard 4-chlorobenzoic acid at 1000 ng/mL ...
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bioRxiv - Molecular Biology 2019Quote: ... All experiments were repeated three times as triplicates on a white Greiner CELLSTAR 96-well plate (Sigma-Aldrich). Cel-miR-39-3p was used as a negative control.
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bioRxiv - Biochemistry 2019Quote: ... T47D and MDA-MB-468) two consecutive times in the presence of 6 μg/μl polybrene (Sigma-Aldrich). Cells were selected for 10 days with 2 μg/μl puromycin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... cells in stationary phase were diluted 100 times in YNB medium containing 5 µ g.mL−1 phleomycin (Sigma) and grown at 30°C with rigorous shaking (200rpm ...
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bioRxiv - Immunology 2019Quote: ... The supernatant was centrifuged at 12,000 rpm for 10 min each time by Amicon Ultra (UFC500308) from Millipore for protein concentrate ...
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bioRxiv - Molecular Biology 2020Quote: ... Bound protein was eluted three times for 30 min by incubation with 0.3 mg/ml 3xFLAG peptide (Sigma) in BC100 at 4°C with end-over-end rotation ...
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bioRxiv - Pathology 2020Quote: ... Membranes were washed three times as above and then developed with BCIP/NBT liquid substrate system (Sigma-Aldrich). The protein concentration of the FIV was determined using a BCA assay (Pierce #23227 ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatants were collected and concentrated ten times in an Amicon 10 k centrifuge filter (Millipore Merck, Darmstadt, Germany) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... the plate was washed four times and incubated with HRP conjugated anti-horse whole IgG secondary antibody (Sigma) for 1 hr at RT ...
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bioRxiv - Neuroscience 2019Quote: ... were washed two times with PBS + 0.02% Tween and coated with anti-FLAG M2 monoclonal antibody (#F3165 Sigma). Thoraces or heads of adult flies were cut and stored in lysis buffer containing 20 mM Hepes ...
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bioRxiv - Physiology 2020Quote: ... The remaining precipitate was washed with cold acetone for three times (All reagents from Sigma-Aldrich and Calbiochem). The protein was redissolved in 8M urea and the protein concentration was determined with BCA Protein Assay Kit (P0009 ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min followed by washing three times with PBS and permeabilized with 0.2% Triton X-100 (Sigma) for 10 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were then washed three times with TBS-T and incubated with alkaline phosphatase-conjugated secondary antibodies (Sigma) in 5% milk in TBS-T for 30 min at room temperature ...