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Citations for Millipore Sigma :
3201 - 3250 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the wells were washed three times with PBS to remove non-adherent bacteria and then stained with 0.1% crystal violet (Sigma). The plates were incubated for 30 minutes at RT and washed three more times with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Coverslips were rinsed three times with PBS and blocked for 1 h in 5% BSA (w/v) (Millipore, 126579) in 0.2% Triton-X100/PBS (blocking buffer) ...
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bioRxiv - Developmental Biology 2023Quote: ... Fixed embryos were washed 3 times (5 minutes each) in wash buffer DPBS Tween containing 2% BSA (Sigma, A3311), and permeabilised at room temperature for 20 minutes in permeabilisation buffer 0.5% Triton-X in DPBS ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed 4 times with PBS-T and developed using SIGMAFAST™ OPD (Sigma-Aldrich, Cat No. P9187) for 10 minutes at room temperature ...
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bioRxiv - Genetics 2024Quote: ... Zygotes with visible pronuclei washed 3 times in HTF medium and transferred to KSOM medium (Millipore, MR-106-D) and cultured in low oxygen chamber filled with the mixture gas (5% O2/ 5% CO2) ...
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bioRxiv - Plant Biology 2024Quote: ... anti-FLAG magnetic beads were washed 5 times with cold wash buffer (GTEN buffer plus 0.1 % NP-40 (Sigma) and Roche protease inhibitor (50 ml/ 1 tablet)) ...
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bioRxiv - Microbiology 2023Quote: ... the conjugated antibody was washed several times with W-buffer using an Amicon Ultra-0.5 centrifugal filter unit with 50 kDa cut-off (Millipore-Sigma) and quantified using a NanoDrop spectrophotometer (280 nm) ...
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bioRxiv - Microbiology 2023Quote: ... and the cells were washed three times in PBS and incubated with 150 μg/ml of gentamicin (Sigma-Aldrich) and 50 μg/ml penicillin G (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... in PBS for 10 min at room temperature in the dark and washed 3 times with PBS (#D8537, Sigma). PBS was completely removed and cells were left to dry for 5 min at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... and the pellet was incubated in fresh PBS for 10 minutes three times before cells were blocked by incubation in 0.1% (w/v) BSA (Sigma) in PBS for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed three times with 1 x PBS and mounted using an antifade mounting media (Sigma-Aldrich/Merck). The images were captured using a Zeiss AXIO Observer.Z1 Inverted Fluorescence microscope (Zeiss) ...
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bioRxiv - Bioengineering 2023Quote: ... plates were washed three times in PBS with 0.05% Tween 20 (PBS-T) and then blocked with 1x casein (Sigma) for 2 h at RT ...
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bioRxiv - Microbiology 2023Quote: ... The coverslip was rinsed 3 times with PBS-T and incubated with Alexa Flour conjugated secondary antibodies (Sigma-Aldrich) and Hoechst 33342 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: The CCl4 model of cirrhosis was prepared as previously described.23 Male C57Bl/6 mice were treated three times a week for 18 weeks with 0.1 ml of 40% CCl4 (Sigma) in olive oil by oral gavage.
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bioRxiv - Cell Biology 2023Quote: ... Pooled bone marrow stromal cells (BMSCs) were washed with 3 times with PBS and cultured in αMEM (Sigma, M2279) supplemented with 20% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... The chips were then washed with PBS 3 times before incubation with 0.5% (v/v) glutaraldehyde (Sigma-Aldrich, G6257) in PBS at RT for 10-15 min ...
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bioRxiv - Developmental Biology 2024Quote: ... The embryos were rinsed and permeabilized in PBS-0.1% triton, 3 times 30 min, and incubated in blocking solution (PBS-0.1% triton, 1% donkey serum (D9663, Sigma)) prior to incubating with primary and secondary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... we washed the sections three times and mounted them with Fluorsave mounting medium (345789-20ML, EMD Millipore, ON, Canada). We observed the slides under an Evos FL fluorescent microscope (AMF4300 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated with the primary antibodies in blocking solution for at least 24h at 4°C: The sections were rinsed five times with PBST (0.1% Tween 20 in PBS, Sigma) and incubated with secondary antibodies for 12h at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... Cells were washed three times using normal medium and then they were released into 20 µM MG132 (Sigma-Aldrich) for 1-2 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with PBS (3 times x 5 minutes) and incubated with secondary antibodies and Hoescht 33342 (Sigma-Aldrich, #14533) diluted in 1x Antibody diluent/Block solution for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were washed three times with PBS before permeabilizing them with 0.1% Triton X-100 (Sigma-Aldrich, T8787) in PBS for 60 seconds ...
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bioRxiv - Biophysics 2024Quote: Time-lapse live imaging is performed either in HM or in soft gel (0.5% low gelling point agarose (Sigma) prepared in HM to reduce tissue movement during imaging ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were treated with the following reagents for the indicated times: 200 μg/ml cycloheximide (CHX; Sigma-Aldrich, C1988); 10 μM MG132 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... blots were incubated for 1 hour rolling at RT in secondary antibody which was anti-mouse antibody raised in sheep (Sigma-Aldrich, St. Louis, MO, USA) diluted 1:10,000 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stimulated by FSK (in the concentration range 1-100 μM) for 30 min at RT in the presence of pan-PDE inhibitor IBMX (500 μM, Sigma-Aldrich, St. Louis, MO, USA). The fluorescence signal ...
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bioRxiv - Cancer Biology 2023Quote: ... The top part of the membrane was blocked with 3% non-fat milk in Tris-buffered saline with 0.1% Tween (TBST) followed by incubation at room temperature (RT) for 1 h with mouse monoclonal anti-FLAG primary antibody (Sigma-Aldrich, USA; #F3165, 1:2,000) to detect the overexpressed EPHB1 receptors proteins (130 KDa) ...
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bioRxiv - Microbiology 2023Quote: ... contained in 24-well microplates with flat bottom were fixed with PBS/4% paraformaldehyde for 15 min at room temperature (RT) and permeabilized with 0.1% Triton X-100 (Sigma, Chemical Company, St. Louis, MO, USA) for 15 min at RT ...
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bioRxiv - Microbiology 2024Quote: ... Cells were cells fixed with 4% paraformaldehyde (ProSciTech, Cat. no. C004) in PBS for 30 min at RT and blocked with 2% BSA (Sigma-Aldrich, Cat. no. A8022-10G) in PBS (BSA/PBA ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were then washed and incubated with the appropriate secondary antibody in 5% non-fat dry milk in PBST for 2h at RT and revealed in Chemiluminescent HRP Substrate (Sigma Aldrich, Saint Quentin Fallavier, France).
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated in primary antibody for 12-18 hours at RT (goat anti-ChAT, 1:500, Millipore Sigma and Cat#: AB144P; mouse anti-NeuN Clone A60-AlexFluor 488 conjugated, Millipore Sigma and Cat #: MAB377X). Slices were incubated in donkey anti-goat Alexa Fluor 555 (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... Sodium kit: Sodium Assay Kit (Colorimetric) (Sigma, product code: MAK247). Potassium kit ...
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bioRxiv - Molecular Biology 2022Quote: ... ECL kit (Millipore) was used to display target protein bands.
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bioRxiv - Genetics 2020Quote: ... 200 µg/ml Proteinase K (Proteinase K, recombinant, PCR Grade, 3115879001, Sigma Aldrich)) ...
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bioRxiv - Neuroscience 2021Quote: ... Diluted cDNA was amplified by PCR using the Sybr Green Master Mix (Sigma), and raw threshold-cycle time (Ct ...
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bioRxiv - Molecular Biology 2021Quote: ... the TADA2b cDNA fragment was PCR amplified and cloned in pET15b vector (Novagen) using Nde I and Bam HI sites ...
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bioRxiv - Cell Biology 2019Quote: ... The biotinylated probe used was generated by PCR using biotinylated-16-UTP (Sigma) and primers P3 and P4 ...
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bioRxiv - Biophysics 2019Quote: ... We visualized PCR products and DNA plasmids by 1 % agarose (SIGMA Cat.#A9539) gel dyed with GelRed (BIOTIUM Cat.#41003) ...
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bioRxiv - Immunology 2019Quote: ... Respective cDNA regions were PCR amplified and cloned into the pET24b vector (Novagen) for bacterial expression ...
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bioRxiv - Synthetic Biology 2020Quote: ... the gene encoding CAT was PCR-amplified from pACYC-Duet™-1 (Novagen) to include a 3’ flexible linker (GTSAAAG ...
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bioRxiv - Bioengineering 2019Quote: PCR master mix: 1x KOD Xtreme Hot Start DNA Polymerase buffer (EMD Millipore), 1.2 µM (each ...
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bioRxiv - Genomics 2020Quote: ... a specific FAM digital PCR assay was designed (Sigma-Aldrich, Gillingham, Dorset, UK) targeting the promotor region of RASSF1A and five 5’-CGCG-3’ BstUI recognition sequences ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR product designed for homologous recombination was transformed using lithium acetate (Sigma) as previously described (Tripp et al. ...
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bioRxiv - Cell Biology 2021Quote: ... as well as by quantitative PCR (KAPA Biosystems, Sigma-aldrich, St. Louis MA). The sequencing libraries were clustered on a single lane of a flowcell ...
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bioRxiv - Biochemistry 2021Quote: ... DNA fragments were PCR-amplified with KOD Hot Start Master Mix (Novagen®) from genomic DNA of M ...
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bioRxiv - Synthetic Biology 2021Quote: ... DNA fragments were either PCR-amplified with oligonucleotide primers (Sigma-Aldrich and IDT) or ordered as gBlocks from IDT and cloned into Gateway pENTR plasmids ...
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bioRxiv - Microbiology 2020Quote: ... Inverse PCR using this construct was carried out with KOD polymerase (EMD Millipore) according to manufacturers instructions ...
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bioRxiv - Microbiology 2021Quote: PCR reactions were carried out with either KOD Hot Start DNA Polymerase (Sigma) or iProof DNA Polymerase (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Conventional PCR was performed using KOD Xtreme Hot Start DNA Polymerase (EMD Millipore). The fragments were cloned into pCR-Blunt II TOPO using the Zero Blunt TOPO PCR Cloning Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitate PCR was conducted using KAPA SYBR FAST Master Mix (Sigma-Aldrich, KK4611) on a Roche Lightcycler 96 (Roche Life Science) ...