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Citations for Millipore Sigma :
2851 - 2900 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... cultures were plated onto the appropriate M9 or LB antibiotic plates using a 48 pronged pinner (Millipore Sigma #R2383). The pinner was sterilized in 10% bleach ...
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bioRxiv - Biochemistry 2023Quote: Samples were incubated for 30 min in plates treated with poly-L-Lysine (Sigma, St. Louis, MO, United State). After treatment with PFA (4% in PBS) ...
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bioRxiv - Plant Biology 2023Quote: ... individual 7-day-old Arabidopsis seedlings were placed in white 96-well plates filled with 200 µl reconstitution buffer (2.5 mM MES pH 5.7 [Sigma-Aldrich, Taufkirchen ...
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Contribution of circulating host and microbial tryptophan metabolites towards Ah receptor activationbioRxiv - Biochemistry 2023Quote: HN30 cells were seeded into 12-well plates at 50,000 cells/well and cultured for 48 h in DMEM/F12 medium (Sigma) with 10% fetal bovine serum (Gemini BioProducts) ...
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bioRxiv - Microbiology 2022Quote: ... serial dilutions were plated on BCYE agar plates supplemented with10ug/ml chloramphenicol and the Legionella Selective Supplement GVPN (Sigma) added according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... BeWo cells were cultured for 24 hours on tissue culture treated plates and DMSO (vehicle control, 0.4%) or 40 uM forskolin (Sigma) was added ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were grown on 6-well plates previously coated overnight with a 0.1 mg/ml solution of poly-L-ornithine (Millipore), an important requirement to prevent cell detachment ...
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bioRxiv - Biophysics 2022Quote: ... pellets were resuspended with 90 μL of TE buffer and spread on an SDAA plate (yeast nitrogen base without amino acid 0.67 %, tryptophan 195 μM, agar 2 % (Sigma) and casamino acids 0.5 % (USBio)) ...
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bioRxiv - Genetics 2023Quote: ... at 37°C in a 6-well plate and then 4mL of media was added (High glucose DMEM (Sigma), 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 20–35 clusters were transferred into each well of a Matrigel-coated 12-well plate and cultured overnight in E8 medium with 1.25 μM ROCK inhibitor (#Y0503, Sigma). On day 0 of differentiation ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were manually scraped off plates to perform a plasmid DNA purification using GenElute Megaprep kit (NA0600-1KT, Sigma).
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bioRxiv - Cell Biology 2023Quote: ... Cells were spread on pre-warmed LB Agar plates containing 100 μg/mL ampicillin (Sigma, St. Louis, MO, USA) and the plates incubated overnight at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 5 and 7 hours 100 µL of were transferred from each reaction to a 96-well MultiScreenHTS HV Filter Plate (0.45 µm pore size; Millipore), and the reactions were stopped by vacuum filtering using a Vac-Man 96 Vaccum Manifold (Promega ...
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bioRxiv - Cancer Biology 2023Quote: Five hundred A673 or SKES1 cells transduced with shRNAs targeting KCNN1 or shRNA non-targeting control were seeded in 6-well plates after 72 hours or 7 days of doxycycline treatment (0,5 µg/mL, Sigma). Cells were then incubated for 8 to 10 days under standard conditions (humidified incubator at 37°C and 5% CO2) ...
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bioRxiv - Cancer Biology 2023Quote: EdU staining was performed on glass coverslips in 24-well plates which were precoated with poly-l-lysine (Sigma) and 5 μg/mL mouse laminin (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... membrane at the bottom (Multiscreen HTS 0.45 μm Hydrophobic High Protein Binding Membrane 96-well Filtration plate (Product No. MSIPS4510; Millipore-Sigma) (65 ...
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bioRxiv - Microbiology 2023Quote: ... Each reaction was analyzed using TLC by spotting 0.5 μL on a 20 cm × 729 20 cm PEI-cellulose TLC plate (Millipore). The TLC plates were developed in 1.5 M KH2PO4 pH 3.8 until buffer was 1–3 cm from the top of plate and air-dried at room temperature and exposed to a phosphor-screen before imaging with a Typhoon Trio Variable Mode Imager (GE Healthcare).
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bioRxiv - Bioengineering 2023Quote: ... were transformed with the ligation mixtures and plated on LB plates containing 50 µg kanamycin (LB-Kan) (Sigma-Aldrich). Plasmids from four colonies with the expected electrophoretic pattern after analytical digestion with NcoI/BamHI (FastDigest ...
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bioRxiv - Cancer Biology 2023Quote: ... Dye transfer from DiI-stained ALL cells cultured in transwells towards MSCs cultured in 6-well plates (Sigma Aldrich) was determined after 40 hours by flow cytometry ...
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bioRxiv - Molecular Biology 2023Quote: ... from a 129xC57BL/6J background 40 were routinely cultured on 0.1% gelatin-coated tissue culture plates in Glasgow Modified Eagle’s Medium (Sigma, G5154), supplemented with 10% inactivated foetal bovine serum (Cytiva HyClone SV30160.03) ...
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bioRxiv - Molecular Biology 2023Quote: ... Mid-log cells (OD600 0.3-0.6) were transferred into a new 96-well plate with rLysozyme and PopCulture reagent (EMD Millipore) and allowed to lyse for 0.5-4 hours ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed 4 times with PBS-T and developed using SIGMAFAST™ OPD (Sigma-Aldrich, Cat No. P9187) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Final cell numbers were 6 x 107 cells/ml for large cultures (24-well size transwell plate, Millipore-Sigma) or 2 x 107 cells/ml for small cultures (96-well size transwell plate ...
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bioRxiv - Immunology 2023Quote: ... Final cell numbers were 6 x 107 cells/ml for large cultures (24-well size transwell plate, Millipore-Sigma) or 2 x 107 cells/ml for small cultures (96-well size transwell plate ...
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bioRxiv - Developmental Biology 2023Quote: ... ESCs were plated on 48-well plate and were pre-treated with 1µM of SR141716/Rimonabant (SML0800, Sigma Aldrich) for 1h before being exposed to the dose range of Δ9-THC ...
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bioRxiv - Molecular Biology 2023Quote: ... From this 30ul was used to transduce 0.1 million HUDEP-2 cells taken in 24 well plate with 1% HEPES and 3μg polybrene (Sigma-Aldrich). Spinfection was carried out at 800G for 30 minutes at room temperature ...
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bioRxiv - Physiology 2023Quote: ... Membrane proteins were extracted from myotubes by gently washing tissue culture plates twice with room temperature PBS and then scraping cells from the plates treated with 250 μL of cold Tris-EDTA buffer (10 mM, 1 mM respectively, pH 7.3; Sigma) containing 2% Triton-X100 (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were centrifuged at 14,000 rpm at room temperature for 5 minutes or filtered in multiscreenHTS-HV 0.45 µm 96 well filter plates with PVDF membranes (Merck Millipore) to remove cell debris and nucleic acid aggregates ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Plates were washed twice with 100μL/well HBSS prior to addition of 80μL/well assay buffer (HBSS (Sigma H8264) + 0.1% BSA) ...
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bioRxiv - Immunology 2023Quote: ... then the plates were blocked for 2 hours at room temperature with 2% bovine serum albumin (Sigma-Aldrich A2153) prior to use ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial suspensions were prepared by diluting bacteria collected from LB plates to an OD600 of 1.5 in KK2 ( 2.25 g KH 2PO4 (Sigma-Aldrich) and 0.67 g K2HPO4 (Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: 293T cells were seeded into 6-well plates and transfected with replicon plasmid using Xtreme-Gene9 transfection reagent (Sigma). 24 hours post-transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were split into 6-well plates at a density of 0.5×106 cells/well and subjected to 2µg/mL puromycin (Sigma P8833) for selection of infected cells ...
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bioRxiv - Cell Biology 2023Quote: ... Phosphopeptides were eluted in 1% ammonia and clarified by filtration using a 0.45 μm filter plate (Millipore, Sigma–Aldrich) before being loaded onto Evotip Pure trap columns.
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bioRxiv - Systems Biology 2023Quote: ... Acidified eluates were transferred to MultiScreen®HTS-HV 96-well filtration plates (0.45 μm, clear, non-sterile, Millipore), stacked on 96-well plates and centrifuged for 1 min at 500 ×g to remove in-suspension particles.
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bioRxiv - Cell Biology 2023Quote: ... Phosphopeptides were eluted in 1% ammonia and clarified by filtration using a 0.45 μm filter plate (Millipore, Sigma–Aldrich) before being loaded onto Evotip Pure trap columns.
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bioRxiv - Neuroscience 2023Quote: ... Cells were seeded at a concentration of 110,000/well in 12-well plates on Ø 18mm glass coverslips coated with 0.1 mg/ml poly-ornithine (Sigma) and 1 µg/ml laminin (Corning) ...
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bioRxiv - Immunology 2023Quote: ... TFPlo cells were sorted and incubated in T cell media + 55µM BME containing 1:1 anti CD3/CD28 Dynabeads in a 96 well round bottom plate with either 5µg/mL Actinomycin D (Sigma) in DMSO or DMSO alone (vehicle ...
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bioRxiv - Cancer Biology 2023Quote: ... THLE-2 cells were maintained on plates coated with 10% filter-sterilized collagen from calf skin (Sigma Aldrich, C8919) at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... Single cells of two untreated CeD patients were sorted into 96-well plates containing 2 µl of scRNA-seq catch buffer (0.2% vol/vol Triton X-100 [Sigma] in H2O with 2 U/μl RNase inhibitor [New England Biolabs] ...
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bioRxiv - Cell Biology 2024Quote: ... 50μl of the culture was then transferred to a 384-well glass-bottomed microscopy plate (Matrical Bioscience) coated with 0.25mg/ml Concanavalin A (ConA, Sigma). Cells in the mid-logarithmic phase were adhered to the plates by incubating at room temperature for 15 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... were placed per well of a 24 well plate and then coated with 0.1% gelatin (Cat no. 39465, Sigma) for 10-15 minutes in a 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... and seeded at 8X105-1X106 cells per well in a 6 well plate coated with Matrigel (Millipore Sigma, CLS354277) 24 hours prior to differentiation treatment ...
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bioRxiv - Genomics 2024Quote: ... 12-well plates for differentiation were coated with 15 ug/mL Poly-L-Ornithine (Sigma-Aldrich; Cat. No. P3655) in DPBS ...
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bioRxiv - Neuroscience 2024Quote: ... HEK 293 cells were seeded into 96-well plates at 4,000 cells per well and transfected the following day with PEI Prime linear polyethylenimine transfection reagent (Sigma) at a 1:3 ratio of DNA:PEI ...
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bioRxiv - Biochemistry 2024Quote: ... Proteoliposomes (100 μL) were loaded into the wells of a MultiScreenHTS + HA 96-well filter plate (pore size 0.45-μm, Millipore). Uptake of the radiolabelled substrate was initiated by the addition of 100 μL buffer containing 2.5 μM [14C]-malate (American Radiolabelled Chemicals) ...
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bioRxiv - Immunology 2024Quote: ... Purified LCs or BMDCs (2.105 cells/well) were seeded in Seahorse XF96 culture plate coated with poly-lysine (Sigma). After overnight culture ...
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bioRxiv - Neuroscience 2024Quote: ... were placed in the center of wells from 96-or 24-well plates coated with 0.1mg/mL poly-D-lysine (Sigma) and 3mg/mL laminin (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... Protein digestion was performed using a modified solid-phase-enhanced sample preparation (SP3) based method adapted to a 96 well format in 0.45µM filter plates (Millipore, MSRPN04)50,51 ...
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bioRxiv - Neuroscience 2024Quote: ... Three days before recording non-embedded organoids were placed into CytoView MEA 48-Black (Axion, cat.no. M768-tMEA-48B-5) plate precoated with 0.1 mg/ml poly-D-lysine hydrobromide (Sigma, cat.no.P7886) for 24 hours following 10 µg/ml laminin (Sigma ...