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Citations for Millipore Sigma :
2801 - 2850 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated with primary antibody Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit for detection of TAP-tagged proteins (Sigma-Aldrich, P1291-500UL) 1:1000 dilution in 5 % skimmed milk/PBST overnight at 4 °C followed by washing in 0.1%Tween/TBS ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was detected using the Simple-Western-JESS capillary western blot system (Bio Techne) multiplexed for total protein and chemiluminescence detection of mouse αSFPQ (1:2000; Sigma-Aldrich WH0006421M2, RRID:AB_1843565) or mouse αTIAL1 (1:10 ...
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bioRxiv - Cancer Biology 2023Quote: Proximity ligation assays (PLA) were performed according to manufacturer’s protocol for both flow cytometry and in situ confocal microscopy detection (Sigma, DUO94104 and DUO92013 respectively). For flow cytometry ...
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bioRxiv - Microbiology 2022Quote: ... or manual extraction kits (Invitrogen PureLink genomic DNA kit or the Sigma GenElute kit). Normalised DNA (at a concentration of 0.2 ng/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were blocked and incubated with the following primary antibodies for 1 hour at RT in humidity chamber (anti-CldU, Abcam ab6326, 1:100; anti-IdU, BD 347580, 1:100; anti-ssDNA, Millipore MAB3034, 1:100). After washes with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... then blocked with 5% normal goat serum in 0.5% PBS-T for 2 hr at RT and stained with pre-absorbed rabbit anti-PER (1:1000) 50 and mouse anti-GFP (Sigma G6519, 1:200) in 5% goat serum in 0.5% PBST for at least 48 hr at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... sectioned organoids were washed three times with 1x PBS for 10 minutes at RT before blocking for 1 hour with 3% bovine serum albumin (BSA) (Sigma, Cat. A9418-50G) and 0.1% triton X-100 in 1x PBS ...
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bioRxiv - Biochemistry 2020Quote: ... and subsequently alkylated with 40 mM iodoacetamide in 100 mM Tris (30 min at RT in the dark) (Sigma Aldrich, St. Louis, MO). Samples were diluted 10-fold with 100 mM Tris (pH 8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... for 2 -4 hours at RT with the following antibodies at the following concentrations: rabbit Cone Arrestin (1:3000, Millipore Sigma, cat. #AB15282), chicken B-galactosidase (1:3000 ...
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bioRxiv - Cell Biology 2020Quote: ... gels were incubated for 1.5 hours at RT on a rocking platform with either 20 μg/mL NHS ester-ATTO594 (Sigma-Aldrich, catalog no. 08741) or 20 μg/mL NHS ester-ATTO532 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... in 1× PBS for 30 minutes at RT and subsequently incubated for 1.5 hours in an inert environment with 20 μg/mL maleimide-ATTO594 (Sigma-Aldrich, catalog no. 08717) dissolved in deoxygenated 150 mM Tris-Cl pH 7.4 solution ...
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bioRxiv - Microbiology 2021Quote: ... Organoid sections were air dried and washed three times for 10 minutes at RT followed by blocking and permeabilizing for 60 mins with a solution containing 3% bovine serum albumin (Sigma, Cat. A9418-50G) and 0.1% Triton X-100 in 1XPBS ...
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bioRxiv - Microbiology 2020Quote: ... Cells were fixed for 1 h at RT by addition of 26.6 μL 16% PFA and 4 μg/ml Hoechst 33342 (Sigma-Aldrich, St. Louis, USA) in PBS using standard bore tubing cartridges ...
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bioRxiv - Neuroscience 2022Quote: ... This was followed by SYBR® Green RT-qPCR reaction using KiCqStart™ SYBR® Green qPCR ReadyMix™ (Millipore Sigma, Cat # KCQS00). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... sections were blocked with 5% normal donkey serum containing 0,3% Triton in PBS for 1 h at RT followed by incubation for two overnights at 4°C with mouse anti-acetylated α-tubulin (1:500, Sigma-Aldrich, T7451) and rabbit anti-βIII-tubulin (1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... At 24h, cell cultures were fixed with AntigenFix (DiaPath, via Solmedia) for 15min at RT or 3.6% formaldehyde (Sigma-Aldrich; diluted in PBS), and permeabilized with 0.2% Triton-X100 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... granulocytes were purified by density centrifugation for 25 min at RT at 800 x g through a Histopaque 1199 gradient (Sigma Aldrich, Taufkirchen, Germany). A discontinuous Percoll® gradient (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... pH 7.4) and blocked for 1 h at RT with a serum containing 3% bovine serum albumin (BSA; Sigma−Aldrich, cat. no. A9647), 10% goat serum (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were stained using primary antibodies for 3 h at RT followed by Duolink in situ PLA probes with anti-rabbit MINUS (Sigma-Aldrich, Cat. # DUO92005), anti-mouse PLUS (Sigma-Aldrich ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Sections were then rinsed with PBS (3×5 min at RT) and then cell nuclei were stained with a DAPI solution (Sigma-Aldrich, 1μg/mL) incubated for 5 min at RT ...
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bioRxiv - Neuroscience 2020Quote: ... the sample was infiltrated with epoxy resin by first incubating it for one day at RT in a solution of 25% Durcupan (Sigma, St. Louis, MO) in acetone ...
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bioRxiv - Immunology 2022Quote: ... we washed the wells and treated them for 1 h at RT with 1:30,000 diluted Extravidin-ALP conjugate (Sigma, St. Louis, MI, USA). After washing ...
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bioRxiv - Molecular Biology 2023Quote: ... The Cas9 protein contained an N-terminal FLAG-tag to confirm expression (mouse anti-3x FLAG primary antibody, 1:250 dilution, RT, 3 h, Sigma-Aldrich Cat# F1804). Cells were washed with PBS buffer ...
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bioRxiv - Neuroscience 2022Quote: ... for 1h at RT and then blocked for 1.5h at RT in 0.1% Triton-X and 10% horse serum (catalog#H0146, Sigma-Aldrich, St. Louis, MO, USA) solution ...
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bioRxiv - Physiology 2024Quote: ... pH 7.5 with 0.1% Tween20) at RT for 1 hour and subsequently incubated with primary antibodies overnight at 4°C (Actin (Millipore Sigma; A2228; 1:10000), IP3R2 (Antibody Research Corporation ...
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bioRxiv - Physiology 2024Quote: ... and the membrane was incubated for 2h at RT with rabbit anti-GAPDH antibody (Sigma Aldrich, G9545, diluted 1:2000 in TBS-T) at RT ...
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bioRxiv - Immunology 2023Quote: ... The resulting single-cell suspension was then pelleted at 400g for 10 minutes at 4°C and resuspended in 25mL RT 30% Percoll (Millipore-Sigma GE17-0891-01) in 1X HBSS without calcium or magnesium (Gibco 14185-052) ...
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bioRxiv - Physiology 2023Quote: ... We blocked sections in 5% normal goat serum in phosphate-buffered saline with 0.1% Tween 20 (blocking buffer) for 30 min at room temperature (RT) and then incubated in primary antibody for laminin protein (1:500, Sigma catalog no. L93931) overnight at 4°C ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The samples were then washed three times with PBS for 30 minutes each at RT and then dehydrated using a series of methanol solutions (20%, 40%, 60%, 80%, 100%, Sigma-Aldrich, #179337-4X4L) for 1 hour each at RT ...
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bioRxiv - Neuroscience 2023Quote: ... sectioned organoids were washed three times with 1x PBS for 10 minutes at RT before blocking for 1 hour with 3% bovine serum albumin (BSA) (Sigma, Cat. A9418-50G) and 0.1% triton X-100 in 1x PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were blocked in 1ml 50% Cas-Block / 50% PBST for 1 hour in Wheaton vials at RT in dark followed by incubation with the primary antibody (anti-Acetylated-tubulin, Sigma, T7451, 1:500) in Cas-Block at 4°C overnight in dark ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were washed and incubated 1 h at RT with peroxidase-conjugated secondary antibodies (anti-mouse, Sigma-Aldrich Cat# A4416, RRID:AB_258167; anti-rabbit, Sigma-Aldrich Cat# A6154, RRID:AB_258284) (1/2000) ...
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bioRxiv - Neuroscience 2023Quote: ... for 3h at RT and then blocked for 1.5h at RT in 0.3% Triton-X and 10% horse serum (catalog#H0146, Sigma-Aldrich, St. Louis, MO, USA) solution ...
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bioRxiv - Plant Biology 2024Quote: ... Kit (Sigma). RNA was treated with TURBO DNAse (Thermo ...
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bioRxiv - Biophysics 2021Quote: ... The fixed cells were washed with PBS three times and permeabilized with 0.1% triton-X (Sigma-Aldrich, T9284) in PBS for 15 minutes at RT ...
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bioRxiv - Cell Biology 2020Quote: ... was aspirated and the beads were then washed three times with TBS buffer supplemented with 0,1% digitonin and eluted by competition with 150μg/ml 3xFLAG peptide (Sigma) in TBS + 0,1% digitonin for 30 minutes at 4°C in an end over end shaker.
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bioRxiv - Cell Biology 2019Quote: ... larvae were washed 3 times with 0.1% PBS-Tween20 and incubated in 0.1% w/v collagenase type 1A (Sigma) in PBS at 37°C for 1.5 hours ...
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bioRxiv - Immunology 2021Quote: ... and HLA complexes subsequently eluted five times with 200 mL 1% trifluoracetic acid (TFA, Sigma/Aldrich, cat. # 02031). For the separation of HLA ligands from their HLA complexes ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were washed three times in PBS and permeabilized in PBT (PBS with 0.5% Triton X-100; Sigma) for 20 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... washed 3 times in 1X PBS and incubated for 5 min in 50mM ammonium chloride (Sigma-Aldrich, 254134) before mounting on cavity blades (Marienfeld ...
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bioRxiv - Neuroscience 2021Quote: ... slides were washed three times for 5 minutes with 1X PBS containing 0.1% Tween- 20 (Cat: P9416, Sigma), and two times for 5 minutes with 1X PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were again washed with PBS (three times) and mounted with Fluoromount aqueous mounting medium (Sigma-Aldrich, F4680). The stained sections were imaged using a Leica SP8 confocal laser scanning microscope ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells were then washed three times in PBS and incubated in 250 μL of 2% saponin (Sigma) for 12 min at 37°C in 5% CO2 ...
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bioRxiv - Genomics 2020Quote: ... The osmium solution was prepared fresh every time by mixing 20 μl of 1mM OsO4 (Sigma Aldrich, 201030) with 4μl of 2M NH4Cl at pH 8.8 and 1μl of RNasin Plus RNase inhibitor (Promega ...
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bioRxiv - Microbiology 2020Quote: ... the cell culture supernatants were pooled to concentrate the virus 20 times using 10,000 MWCO spin columns (Millipore), and aliquots were stored at −70°C [14] ...
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bioRxiv - Systems Biology 2019Quote: ... in log-phase growth (OD = 0.5) was concentrated 10x times (OD = 5) by a glass filter system with a 0.45 μm filter (Millipore). For osmolyte step experiments ...
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bioRxiv - Molecular Biology 2019Quote: ... The plates were washed 3 times with pre-cooled PBS prior to the addition of L-DOPA (Sigma). After incubation in L-DOPA for 1 day ...
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bioRxiv - Immunology 2020Quote: ... coated plates were washed one time with 100 μl/well PBS with 0.05% Tween 20 (PBST) (Sigma-Aldrich), blocked with 100 μl/well of 1% human serum albumin (HAS ...
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bioRxiv - Cell Biology 2019Quote: ... Immune complexes were washed two times with lysis buffer and eluted with 0.5 mg/mL Flag peptide (Sigma) in lysis buffer ...
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bioRxiv - Bioengineering 2020Quote: ... Fixed collagen samples were washed 3 times with a solution containing 50 mM PIPES pH 7.4 (Sigma, P6757) kept in ice and then they incubated for 2h in ice and in the dark in a freshly prepared staining solution (SSI ...