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Citations for Millipore Sigma :
2601 - 2650 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... was added for 1h at RT followed by incubation with OPD (o-phenylenediamine dihydrochloride) revelation substrate for 10 min (Sigma-Aldrich, #P8287). Absorbances were analyzed at 492 vs 620 nm on an ELISA plate reader (Berthold).
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bioRxiv - Microbiology 2024Quote: ... Some antibodies used were directly coupled to HRP and incubated 1 h at RT (FLAG-HRP, Sigma-Aldrich, A8592, 1/5000; Myc-HRP, Sigma-Aldrich, A5598 ...
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bioRxiv - Cell Biology 2023Quote: ... for 15 min at RT and stained with filipin (50 μg/mL in PBS prepared freshly from a 250-fold ethanolic stock; #F9765, Sigma-Aldrich/Merck) for 2h at RT in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... 137 mM NaCl2) before blocking for 1 hour at RT with blocking solution (2% BSA IgG free and protease free (Sigma-Aldrich, A3059); 0.1 % Tween 20 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... incubated in 70% EtOH for 1 hour on a nutator at RT and rinsed in demi-water and stained overnight (O/N) in carmine solution (1g carmine (Sigma, cat. #C1022), 2.5 g aluminum potassium sulphate (Merck ...
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bioRxiv - Genetics 2024Quote: ... the slides were washed in 1x PBS for 5 min at RT and incubated in 0.005% Pepsin (Sigma-Aldrich, Cat# P7012-250MG) in 0.01 N HCl solution for 10 min at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed twice with PBS and permeabilized at RT for 20 minutes using 0.5% w/v saponin (Sigma Aldrich, Cat # 47036), 0.5% v/v Triton X-100 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... slides were dried in dark conditions at RT and a glass coverslip was mounted on top of each slide using Fluoromount mounting medium (Sigma Aldrich, USA). Z-stack images with a step size of ∼ 2 μm were acquired with an UPlanSApo 20× or a PlanApoN 60x objective using an inverted confo-cal microscope (FV1200 ...
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bioRxiv - Cell Biology 2023Quote: ... Fixation was followed by blocking (1h, RT) with 3% bovine serum albumin (BSA/0.01% Tween™20/PBS) (Sigma, Cat#A-7906). Samples were incubated with primary antibodies (overnight ...
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bioRxiv - Biochemistry 2023Quote: ... 500 μL of each lysate sample was incubated for 1 h at RT with 10 μL of 10 mM biotin picolyl azide (in DMSO) (Sigma Aldrich 900912), 10 μL of 50 mM TCEP (in H2O) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated for 7 days to analyze gene expression by RT-qPCR and 21 days for calcium deposition staining with 2% alizarin red (Sigma-Aldrich, #A5533). For this purpose ...
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bioRxiv - Microbiology 2023Quote: ... in PBS and subsequently blocked and permeabilized for 1 h at RT in PBS supplemented with 50 mM ammonium chloride (Sigma-Aldrich, 254134), 0.1% saponin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Enucleated eyes were immersion fixed in 4% PFA diluted in 1x PBS for 1h at RT and incubated in a hydrogel solution: 4% acrylamide (Sigma, Cat# A4058), 2% bis-acrylamide (Alfa Aesar Cat# J63265) ...
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bioRxiv - Cancer Biology 2023Quote: ... HUVECs on gelatin-coated plates were incubated with gcEVs labeled with the fluorescent dye PKH-26 (Sigma-Aldrich, 10 min at RT). After washing ...
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bioRxiv - Plant Biology 2023Quote: ... A 90-min incubation at RT on a tube rotator was followed by supplementation with 10 mg/mL collagenase type II (Sigma-Aldrich, USA) in Ca2-free OR2 and ...
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bioRxiv - Plant Biology 2023Quote: ... cerevisiae was isolated from liquid YPD cultures 8 hours after inoculation from single colonies using the formamide-EDTA method (59) followed by RNAzol® RT (Sigma-Aldrich) extraction ...
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bioRxiv - Neuroscience 2023Quote: ... pH 8.6) for 30 min at RT before incubation with blocking solution consisting of 3% bovine serum albumin (Sigma, Merck, Germany, #A9418) and 0.3% Triton X-100 in PBS 1X ...
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked in TBS-T with 5 % non – fat milk for 1 hour in RT following incubation overnight at 4 °C with primary (1:1000) antibodies: Flag (Sigma, Cat. F1804), IL-1β (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated at RT for 1 h with fluorescently labelled secondary antibodies and Hoechst-33342 (Sigma-Aldrich, cat.no. B2261-25MG), which were diluted in blocking buffer as well.
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bioRxiv - Cell Biology 2023Quote: ... Cells were blocked in 3% BSA for 30 min at RT and incubated with anti-alpha-SMA antibody (1:1000; Sigma, Cat. # A2547) and anti-collagen type I antibody (1:1000 ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were blocked 1 hr in antibody buffer at RT and incubated depending on the experiment with following primary antibodies: Anti- Acetylated Tubulin (1:1000, Sigma-Aldrich, #T6793); anti ZO-1 (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... 450 µg of total protein were incubated for 20 min (rotating wheel, RT) with 50 µL of streptavidin-agarose beads (Sigma-Aldrich, #S1638). After incubation ...
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bioRxiv - Neuroscience 2024Quote: ... the samples were incubated with fluorescent dye-conjugated secondary antibodies at RT for 2 hours with 1% NDS in PBST with 4’,6-diamidino-2-phenylindole (DAPI, 1:1000, Sigma-Aldrich, D9542). Afterward ...
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bioRxiv - Neuroscience 2024Quote: ... Mice were anesthetized with isoflurane and then perfused transcardially with RT 0.9% saline with 10 units/mL heparin (Sigma Aldrich, H3393-50KU) followed by RT 4% PFA in 0.2M phosphate buffer (PB) ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed 3 times with PBST and 100 μL of o-phenylenediamine dihydrochloride (SigmaFast OPD, Sigma) substrate was added per well ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed 3 times with PBST and 100 µL of o-phenylenediamine dihydrochloride (SigmaFast OPD, Sigma) substrate was added per well ...
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bioRxiv - Neuroscience 2021Quote: ... and a third time in PBS-Igepal-PMSF (phenylmethylsulfonyl fluoride) solution (0.5% Igepal, 1 mM PMSF (Sigma) in 1X PBS pH 7.4) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were treated from the time of plating with Tyrphostin-AG1478 (80 nM in ethanol, Sigma-Aldrich), Erlotinib (100 nM in DMSO) ...
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bioRxiv - Developmental Biology 2021Quote: ... rinsed several times with H2O followed by Reynolds lead citrate in H2O (Sigma, St Louis, MO, USA) for 10 min and rinsed several times with H2O ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were incubated for indicated times and then cells were resuspended in TRI Reagent (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2021Quote: ... Fixed sections were gently rinsed two times with 1xPBS and treated with pepsin reagent (Sigma-aldrich, R2283) for 5 min at 37°C ...
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bioRxiv - Physiology 2022Quote: ... Methanol was removed and cover slips were rinsed 5 times with phosphate buffered saline (PBS, Sigma-Aldrich) to remove excess methanol ...
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bioRxiv - Molecular Biology 2021Quote: ... The samples were washed three times in PBT (PBS with 0.1% Triton™ X-100 (Sigma, T8787) and 0.2% BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... a 500 μl 50% suspension of 3 times pre-washed streptavidin agarose beads (Novagen, Cat. N°69203) (i.e. ...
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bioRxiv - Cell Biology 2022Quote: ... Stained cells were washed three times with PBS containing 4’,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich). Image were taken with a FLUOVIEW FV1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were washed six times for 20 minutes each in PBS with 0.1% Tween-20 (Sigma P1379) and incubated for 1 hour with Alexa Fluor conjugated secondary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... Myocultures were then washed three times with 1× PBS supplemented by 0.1% Tween20 (Sigma-Aldrich, Cat. P9416). The culture inserts were removed to allow mounting with a top coverslip and appropriate mounting media ...
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bioRxiv - Immunology 2021Quote: ... The plates were then washed six times with wash buffer (0.002% (v/v) Tween-80 (Sigma Aldrich) in PBS) ...
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bioRxiv - Cell Biology 2019Quote: ... cells were washed two times with PBS and adipocytes were incubated with Oil Red O (Sigma #O0625) for 5 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were washed three times with TBST and then incubated with horseradish peroxidase-conjugated secondary antibody (Millipore) for 1 hr at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... Cells were washed with PBS 3 times and incubated with fluorophore-conjugated secondary antibody TRITC (Sigma Aldrich, St ...
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bioRxiv - Immunology 2019Quote: ... 100% methanol for 1 hr for three times and a mixture of 1-part benzyl alcohol (Sigma): 2-parts benzyl benzoate (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... rinsed several times with water followed by Reynolds lead citrate in water (Sigma, St Louis, MO, US) for 10 mins and rinsed several times with water ...
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bioRxiv - Biochemistry 2020Quote: ... dissolved in water and then buffer exchanged at least three times using a centrifugal concentrator (EMD Millipore) into the desired buffer.
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bioRxiv - Cancer Biology 2020Quote: ... Plates were then washed 8 times and avidin-peroxidase (2.5 μg/mL in PBS / 10 % FCS. Sigma) was added for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: γ-secretase inhibitors used in this study were as follows: avagacestat (10 μM, 24 h treatment time, Sigma), N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine-1,1-dimethylethyl ester (DAPT ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed in PBS three times for 10 min each and incubated with DAPI (Sigma) to label nuclei for 5 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Beads were washed three times and FLAG-tagged proteins eluted using 3X FLAG peptide (Sigma; cat. F4799). Rhodamine-Azide labeling reactions were performed using 1.7 mM TBTA ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were then buffer exchanged at least three times using a centrifugal concentrator (EMD Millipore, 3kDa cutoff) into the annealing buffer ...
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bioRxiv - Neuroscience 2021Quote: ... 2 times 20 min with 50% formamide/2X standard saline citrate - 0.1% Tween 20® (Sigma Aldrich), SSCT ...