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Citations for Millipore Sigma :
2751 - 2800 of 10000+ citations for Ribonuclease P Protein Subunit p38 RPP38 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The plates were washed with PBST and reactions were developed with p-nitrophenyl phosphate substrate (PNPP; Sigma-Aldrich, N2765). Plates were read at 405 nm absorbance ...
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bioRxiv - Plant Biology 2020Quote: ... Leaf tissue was ground in extraction buffer (150 mM NaCl, 50 mM Tris [pH 7.5], 0.2 % Nonidet P-40 [Sigma-Aldrich] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Samples were derivatized in RT for 3 min with 10 μl of 60% p-toluenesulfonyl isocyanate (PTSI) (Sigma Aldrich) in ACN (Hu et al. ...
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bioRxiv - Physiology 2020Quote: ... osteoblast differentiation capacity was assessed by alkaline phosphatase (ALP) staining using SIGMAFAST™ p-Nitrophenyl phosphate tablets (N1891, Sigma). Colonies with more than 10% of ALPpositive cells were considered as colony-forming units–osteoblasts (CFU-OBs) ...
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bioRxiv - Cell Biology 2020Quote: ... 2% horse serum and 1% P/S) supplemented with Gibco™ B-27™ Supplement (50X, 1:50, Sigma) for a further 10 days.
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bioRxiv - Biochemistry 2021Quote: ... we first prepared three solutions of 1) 5 mM ALP-TCO and 1 mg/mL p-NPP (Millipore-Sigma), 2 ...
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bioRxiv - Biochemistry 2021Quote: ... we first prepared three solutions of 1) 5 mM ALP-TCO and 1 mg/mL p-NPP (Millipore-Sigma), 2 ...
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bioRxiv - Biochemistry 2021Quote: ... The Ape1-thrombin mixture was incubated with p-aminobenzamidine-agarose beads (5 mg thrombin:1 mL beads ratio; Sigma) at 4 °C for 15 min to remove thrombin and was then filtered through a 0.22 μm PVDF membrane ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma], 10 mM NaCl [Sigma], 0.2 % Nonidet P-40 substitute [Sigma] ...
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bioRxiv - Cell Biology 2021Quote: ... Protein samples were separated on a 12.5% SDS-PAGE gel and transferred to a polyvinylidenedifluoride (PVDF) membrane (Immobilon-P, Millipore). Blots were blocked with 3% non-fat milk-0.05% Tween-20 in Tris buffer saline (TTBS ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed with PBST and reactions were developed with p-nitrophenyl phosphate substrate (PNPP, Sigma-Aldrich, N2765). Plates were read at 405 nm absorbance and antibody titers were calculated as the highest serum dilution with an OD value above 2 times the average OD of the negative controls.
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bioRxiv - Microbiology 2021Quote: ... The cells yielded in this way were cultured in DMEM supplemented with 10% FBS and 1% P/S (Sigma). Primary cells were immortalized by transfection of pRSVAg1 plasmid expressing Simian Vacuolating Virus 40 large T antigen (SV40T ...
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bioRxiv - Immunology 2021Quote: PBMCs from donors previously screened for ex vivo CD4+ T cell reactivities against MHC-class II peptides were thawed and rested in T cell medium (TCM; IMDM + 1x P/S + 50μM β-ME + 10% h.i. HS) containing 1μg/mL DNase I (Sigma-Aldrich) at a concentration of 2-3×106 cells/mL for 3h at 37°C and 7.5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... IP samples were resolved on 8% or 10% SDS-PAGE gels and transferred onto Immobilon-P membranes (Merck Millipore) using a semidry transfer apparatus ...
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bioRxiv - Molecular Biology 2021Quote: ... were lysed with a Potter-Elvehjem homogenizer in lysis buffer (10 mM Tris pH 6.8, 10 mM MgCl2, 0.2% Nonidet P-40, 1 mM PMSF (Sigma-Aldrich), 4 mM benzamidine (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2023Quote: ... above the tips with the help of forceps and placed into a 35-mm-diameter dish containing a 70 µm strainer and 3 ml enzyme solution (1.25% [w/v] Cellulase [“ONOZUKA” R10, Yakult], 0.1% [w/v] Pectolyase [P-3026, Sigma-Aldrich] ...
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bioRxiv - Molecular Biology 2022Quote: ... by electrophoresis and subsequently transferred onto Immobilon-P™ PVDF membranes of 0.45 μm pore size (Millipore®; Merck). Details of primary antibodies used are provided in Supplementary Table 2 ...
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bioRxiv - Immunology 2024Quote: ... 70 μL of alkaline phosphatase substrate (5 mM p-nitrophenyl phosphate substrate in 0.1 M alkaline buffer; Sigma-Aldrich) was added and color was allowed to fully develop ...
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bioRxiv - Immunology 2024Quote: ... Pancreas tissue was minced with a blade and digested in 1mg/mL of collagenase P (Millipore Sigma, Rockville, MD) and 0.1mg/mL of DNAse (Millipore Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... 6.5 x 104 cells were seeded on to individual Poly-DL-Lysine (25-40 kDa P-DL-L;0.1 mg/ml; Sigma) coated 12 mm glass coverslips or P-DL-L coated 8-well NunC Chambers (Thermo Fisher Scientific) ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: Recombinant human MMP-2 (R&D) and MMP-9 (R&D) were activated using p-aminophenylmercuric acetate (APMA, Sigma) in MMP assay buffer (50 mM Tris (Fisher) ...
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bioRxiv - Immunology 2024Quote: ... 70 μL of alkaline phosphatase substrate (5 M p-nitrophenyl phosphate substrate in 0.1 M alkaline buffer; Sigma-Aldrich) was added and plates were incubated at room temperature to allow color development ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragmented tissue was then digested with 5 mL (0.4 mg/mL) collagenase P solution (Sigma, Cat. No. 11213857001) in RPMI1640 (Sigma ...
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bioRxiv - Immunology 2023Quote: ... The excised meninges were enzymatically digested with Collagenase P (1 mg/ml) and DNAse I (1 mg/ml; Sigma) in 1X PBS (HSBB ...
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bioRxiv - Cancer Biology 2024Quote: Tissue biopsies were carefully cut into small pieces and digested using collagenase P at 200 μg/ml (Sigma-Aldrich), dispase II 800 μg/ml (thermofisher scientific ...
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bioRxiv - Neuroscience 2023Quote: ... or 15 minutes (P18-30) in oxygenated aCSF solution at 35°C containing 0.01-0.02% Collagenase P (Sigma, 11213857001). RuBi-GABA (Fisher ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples were analyzed on an SDS–polyacrylamide (37.5:1) gel electrophoresis and blotted on 0.45 µm pore PolyVinyliDenFluoride (PVDF) membranes (Immobilon-P from Sigma Aldrich). Membranes were blocked 2h with 1X Tris-buffered saline (TBS ...
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bioRxiv - Immunology 2023Quote: ... lavage fluids (1/2 diluted; 100 µl) were incubated with 500 µM Gly-Pro-p-nitroanilide substrate (Sigma-Aldrich) in 200 mM Tris-HCl buffer (pH 8.3 ...
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bioRxiv - Bioengineering 2022Quote: ... 3 mM aniline dimer solution was prepared by dissolving 5.5 mg of N-phenyl-p-phenylenediamine (Sigma-Aldrich, 241393) in 10 mL of Tyrode’s solution ~20 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... the pancreatic duct was cannulated and distended with 4°C collagenase/protease solution using Collagenase P (Sigma-Aldrich, USA) in 1x Hank’s balanced salt solution and 0.02% bovine serum albumin ...
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bioRxiv - Neuroscience 2023Quote: 17β-Estradiol (E2, E8875), β-Estradiol-3-Benzoate (EB, E8515) and Progesterone (P, P0130) were purchased from Sigma-Aldrich and dissolved in sesame oil ...
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bioRxiv - Bioengineering 2023Quote: ... the bound proteins were detected by adding 100μL of 1 mg/mL p-nitropenylphosphate substrate tablets dissolved in diethanolamine buffer (pH 9.8) (Sigma-Aldrich). The absorbance was measured at 405 nm using the Sunrise spectrophotometer (Tecan).
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bioRxiv - Biochemistry 2023Quote: ... the nuclear lysate was obtained by resuspending the nuclear pellet in PBS containing 0.1% Nonidet P-40 (NP-40) and 1% protease inhibitor (#P8340, Sigma). Both the nuclear and cellular fractions were sonicated for 10 seconds of two cycles each with a holding period of 5seconds to cool down and then separated by SDS-PAGE and immunoblotted for TRF2 and TERF2IP ...
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bioRxiv - Cell Biology 2023Quote: ... coverslips with cells were fixed and permeabilized as described previously and mounted in a homemade mounting medium (90% glycerol, 100 mM Tris, pH 8.0, and 0.1 mg/ml p-phenylenediamine [695106; Sigma-Aldrich]).
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bioRxiv - Microbiology 2023Quote: ... The assay starts by addition of 100 µl of 0.4% pNP (p-nitrophenyl phosphate in 1 M Tris-HCl, pH 8.0; Sigma-Aldrich) to each sample and incubation at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... SDS samples were subjected to SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to Immobilon-P membranes (Millipore, IPVH00010). The membranes were blocked with 5% skim milk (Megmilk Snow Brand ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid-PEI solutions were added to 293-EBNA1 cells (2 x 106 cells/mL) in Expression Medium [F17 Medium supplemented with 0.1% Kolliphor P 188 (Sigma), 6 mM L-glutamine (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... and then with dH20 (3x) before the addition of substrate solution (1 mg/mL p-nitrophenyl phosphate) in 0.2M TRIS buffer (both Sigma). Optical density absorbance readings were determined using a Thermo Multiskan EX plate reader (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 1% P/S and 1 μg/mL TPCK trypsin (T1426, Sigma-Aldrich Inc., Saint-Louis, MO, USA). The culture supernatant from the infected cells was cleared by centrifugation (2,500 ×g ...
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bioRxiv - Plant Biology 2024Quote: ... primary root tips were cut and placed into a 35 mm petri dish containing a 70 um strainer and 4 mL enzyme solution (1.25% [w/v] Cellulase [“ONOZUKA” R-10, Yakult], 0.1%[w/v] Pectolyase [P-3026, Sigma-Aldrich] ...
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bioRxiv - Molecular Biology 2024Quote: SDS-PAGE (10%) was followed by a semidry blotting method using PVDF membrane (Millipore Immobilon-P membrane, 0.45 µm). For blocking 5 (m/v ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellet was resuspended in culture medium (DMEM, 10% FCS, 1% P/S) and plated on collagen-I (0.025%, Sigma) and 100 µg/ml poly-ornithine (Sigma)-coated plastic dishes for RNA and protein analysis (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... BvgA was phosphorylated by incubation in the presence of 20 mM lithium potassium acetyl phosphate (Ac∼P; Sigma-Aldrich) in 20 mM Tris-Cl ...
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bioRxiv - Neuroscience 2024Quote: ... to a resistance of 3.5 – 5.0 MΩ using a P-1000 microelectrode puller and filled them with 300 μM glutamate (Sigma, # G5889) dissolved in a vehicle solution containing (in mM) ...
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bioRxiv - Physiology 2024Quote: ... Maximum oxygen consumption was also measured in the presence of 1 μM carbonylcyanide-p-trifluoromethoxyphenylhydrazon (FCCP, Sigma Aldrich C2920).
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bioRxiv - Bioengineering 2024Quote: ... mix 1 mL of 3.34 mM [Ca] solution (from CaCl2) with 1 mL of 19 mM [P] solution (a mixture of Na2HPO4 (Sigma) and 300 mM NaCl (Sigma)) ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1% Nonidet P-40) containing 10 mg/ml BSA at the following dilutions: mouse anti-α-tubulin (Sigma, T9026) (primary ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were terminated by spotting 15 μL onto phosphocellulose paper (SVI-P; St Vincent’s Institute, Melbourne) and washing extensively in 1% phosphoric acid (Sigma). Radioactivity was quantified by liquid scintillation counting ...
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bioRxiv - Biochemistry 2021Quote: ... protein standards (Merck Sigma-Aldrich) specified in the Supplementary Material were separated on the same column.
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bioRxiv - Biochemistry 2020Quote: ... Protein A/G beads (Millipore) were then added for overnight incubation at 4°C ...