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Citations for Millipore Sigma :
2701 - 2750 of 10000+ citations for Ribonuclease P Protein Subunit p38 RPP38 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Tetramer staining was performed in combination with staining for chemokine receptors CXCR5 and CXCR6 for 1 h at RT in staining buffer supplemented with Dasatinib (50 nM) and biotin (20 nM; Sigma, B4501). After washing of the samples with FACS buffer (PBS supplemented with 1 % FCS) ...
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bioRxiv - Microbiology 2024Quote: ... followed with 100 µl per well of either 1:3000 diluted biotin goat anti-mouse IgG (in 0.5% skim milk PBST; Sigma SAB4600004-250µl) or 1:1000 diluted biotin goat anti-mouse IgA (BD ...
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bioRxiv - Biophysics 2024Quote: ... 50 mL of blocking buffer was prepared per 2 x 108 cells by adding 1 g of biotin-free BSA (Sigma Aldrich) and 200 mL of 0.5 M pH 8.0 EDTA into DPBS (GIBCO) ...
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bioRxiv - Biophysics 2024Quote: ... we wash the chambers thrice with Phosophate-buffered saline (PBS) and incubate them for at least 5 minutes with BSA-biotin (0.5 mg/ml, Sigma Aldrich, USA), followed by 5 minutes with NeutrAvidin (0.2 mg/ml ...
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bioRxiv - Immunology 2021Quote: ... the membranes were washed in PBS-T for 40 min and the bound proteins were visualized with anti-FLAG-HRP antibodies (1:10000 in PBS-T for 1h, Sigma-Aldrich, Cat. no: A8592). All steps were performed at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Flat-mounted retinas were incubated overnight at 4°C with antibodies against RNA-binding protein with multiple splicing (RBPMS; EMD Millipore Corp., Burlington, MA) diluted 1:500 in blocking buffer (2% bovine serum albumin ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Molecular Biology 2021Quote: ... 500μg of lysates in 500 μL of SEC buffer were incubated for 3 hours with primary antibodies directed to either AGO proteins (WAKO anti-AGO2 #011-22033, EMD Millipore anti-panAGO #MABE56) or directed to T6B-fusion protein (Cell Signaling anti-FLAG #8146S ...
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bioRxiv - Neuroscience 2020Quote: ... anti-amyloid precursor protein (APP; mouse anti-human monoclonal antibody clone 22c11, dilution 1:10, epitope retrieval time 10 min, MAB348; EMD Millipore, Burlington, MA, USA); anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Molecular Biology 2023Quote: ... Histidine tagged PDE4 and GST tagged CP proteins were detected chromogenically using anti-mouse secondary antibodies labelled with alkaline phosphatase (1:30000) (cat#A3562, Millipore Sigma, Burlington, MA, USA) and western blue stabilized substrate for alkaline phosphatase (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... Labelled RNAs in the eluate were then detected by autoradiography and proteins were subjected to WB using an anti-Flag antibody (Sigma-Aldrich F3165; 1:10000).
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bioRxiv - Molecular Biology 2022Quote: ... and expression levels of AGAL-FLAG tagged proteins were assessed by Western blot with mouse monoclonal anti-FLAG antibody (F1804, SIGMA-Aldrich, Prague, Czech Republic). Clones demonstrating similar GLA_FLAG transcript and AGAL-FLAG protein amounts were selected for further analyses.
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bioRxiv - Molecular Biology 2023Quote: ... The expression of 3 × FLAG-tagged MpRBOHB proteins was confirmed by western blot analysis using an anti-3 × FLAG antibody (Sigma-Aldrich, Cat. No. F1804).
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bioRxiv - Neuroscience 2024Quote: ... and a primary rat antibody against myelin basic protein (MBP) (1:50 in blocking solution; Cat. No. MAB386, Sigma-Aldrich, St. Louis, MO, USA). Following five 5-minute washes at 20°C with PBS ...
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bioRxiv - Neuroscience 2024Quote: ... and a primary rat antibody against myelin basic protein (MBP) (1:50 in blocking solution; Cat. No. MAB386, Sigma-Aldrich, St. Louis, MO, USA). The following day ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were normalized to either actin or GAPDH housekeeping proteins with monoclonal antibodies (anti-actin; 1∶1000, Sigma or anti-GAPDH; 1:10,000 Abcam).
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... Cells were then fixed using 10% formalin and processed for immunofluorescence using an antibody against the HCoV-OC43 N protein (Millipore #MAB9013; diluted 1:500) and a secondary Alexa Fluor 555 conjugated anti-mouse IgG antibody (NEB #4413S ...
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bioRxiv - Microbiology 2020Quote: ... protein complexes were precipitated using Protein A agarose beads (Sigma, St. Louis, MO, USA). The precipitated material was analyzed by western blot ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: The protein content was analysed with the Bicinchonic Acid Protein Assay Kit (Sigma-Aldrich) according to the manufacturer’s protocol with minor adjustments ...
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bioRxiv - Cell Biology 2021Quote: The Protein A slurry was prepared by washing 1.5 g Protein A Sepharose (Sigma) 4 times in 1X Tris Buffered Saline (1X TBS ...
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bioRxiv - Neuroscience 2021Quote: ... Bradford protein assay was used to quantify total protein levels (Bradford, Sigma-Aldrich, USA) and to normalize BDNF data.
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Bound proteins were captured with Protein G agarose for 4 h (Sigma-Aldrich, #17061801), spun at low speed ...
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bioRxiv - Molecular Biology 2024Quote: Total carbonylated proteins were quantified using OxyBlot Protein Oxidation Detection Kit (Millipore, Billerica, MA) following manufacturer’s instructions using 15 μg of total protein detected with rabbit anti-DNP antibodies (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... 500 μg of protein were added to washed agarose protein A beads (Merck Millipore) and incubated on a rotating wheel for 1 h at 4°C ...
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bioRxiv - Physiology 2022Quote: ... Protein carbonylation was assessed with an Oxyblot protein oxidation detection kit (#S7150, Merck Millipore) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and total (i.e. nonphosphorylated) protein levels were normalized to tubulin protein levels (Sigma-Aldrich, #T6557 ...
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bioRxiv - Microbiology 2024Quote: ... and visualized using InstantBlue Coomassie Protein Stain or ReadyBlue Protein Gel Stain (Sigma Aldrich).
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bioRxiv - Molecular Biology 2024Quote: ... Protein amounts were measured using the bicinchoninic acid assay (BCA Protein Assay Kit, Novagen) on a NanoDrop ND-1000 instrument (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2024Quote: ... The resulting protein mixtures were filtered using a low protein binding filter (Millipore Sigma), and purified through a Source 15Q anion exchange column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were either stained for protein content using ReadyBlue Protein Gel Stain (Sigma-Aldrich) or subjected to in-gel peroxidase activity assays following a modified ferricyanide protocol.80 In-gel detection of peroxidase activity was initiated by incubating gels with a 0.05% 3,3’-diaminobenzidine (DAB ...
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bioRxiv - Developmental Biology 2020Quote: ... transgenic and mutant zebrafish embryos were raised at 28°C in Embryo Water (Volvic© water containing 0.28 mg/mL Methylene Blue [M-4159; Sigma] and 0.03 mg/mL 1- phenyl-2-thiourea [P-7629; Sigma]), and staged according to Westerfield (Westerfield ...
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bioRxiv - Microbiology 2020Quote: ... in M9 minimal medium supplemented with 0.4% D-glucose and 10 mM para-chloro-phenylalanine (p-Cl-Phe; Sigma) for B ...
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bioRxiv - Plant Biology 2020Quote: ... and ground in liquid N2 in 2 volumes of extraction buffer (50 mM Tris–HCl pH 7.5, 150 mM NaCl, 10% glycerol, 0.1% Nonidet P-40 and 1x protease inhibitor cocktail; Sigma-Aldrich) and centrifuged for 12,000 g for 10 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates containing FLAG-tagged motors were diluted in assay buffer [AB: P12 buffer (12 mM PIPES (Sigma P-1851), 1 mM EGTA (Sigma E-4378) ...
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bioRxiv - Cell Biology 2021Quote: ... and were transferred at 100 mA for 70 min onto PVDF membrane (Immobilon-P, 0.45 μM pore size, Merck Millipore) using semi-dry blotting system ...
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bioRxiv - Neuroscience 2021Quote: ... samples were heated 5 minutes at 95°C and 30µl were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilion-P, Merck Millipore) according to standard protocols ...
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bioRxiv - Microbiology 2021Quote: ... and applying lysis buffer (20 mM Tris/HCl, pH 7.5, 150 mM NaCl, and 1% Nonidet P-40 (all from Sigma)) supplemented with EDTA-free protease inhibitor mixture cocktail (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... the cells were incubated with 100 μl p-nitrophenyl phosphate solution as advised by the manufacturer (Sigma-Aldrich N1891). Fluorescence was measured at an excitation of 440±25 nm and an emission of 550±35 nm.
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bioRxiv - Cell Biology 2021Quote: ... Tissues were digested in 10 mL Collagenase P 5 U/mL (11 213 873 001, Sigma Aldrich, Buchs, Switzerland) for 40 minutes at 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... cells were rinsed twice in DPBS then simultaneously blocked and permeabilized with B/P buffer [DPBS with 0.1% Tween 20 (P2287; Sigma), 0.1M glycine (161-0718 ...
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bioRxiv - Biophysics 2022Quote: ... The sample was reduced by adding 3.5 μl of a mixture of 1.4 mM N,N,N’,N’-tetramethyl-p-phenylenediamine (TMPD, Millipore sigma) and 112 mM ascorbate (chem-impex international ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by wet transfer (stacking and resolving gel) in transfer supplemented with 20% methanol on 0.45mm PVDF membrane (IMMOBILON-P, Millipore). PVDF was selected over nitrocellulose as it shows an improved transfer of free SUMO (Castoralova et al. ...
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bioRxiv - Neuroscience 2020Quote: ... 20 μg total protein/samples were loaded in each lane of each gel on 7.5-10% SDS-polyacrylamide gel and then transferred to nitrocellulose membrane (Immobilon-P, Millipore). After blocking ...
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bioRxiv - Cell Biology 2021Quote: ... Denaturated and fully-reduced proteins were resolved on Tris-glycine SDS-PAGE followed by western blot analysis onto PVDF membrane (Immobilon-P, 0.45 μM pore size, Merck Millipore) using enhanced chemiluminescence to detect bound antibodies (Pierce) ...
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bioRxiv - Microbiology 2021Quote: ... lysed in Laemmli SDS-loading dye and separated in 15% acrylamide gels and transferred to 0.45 μm hydrophobic Immobilon-P PVDF membrane (Merck Millipore). Immunoblotting was as described previously 19 ...
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bioRxiv - Physiology 2021Quote: ... The procedure consisted of using one mM of BAPNA (Nα-benzoyl-DL-arginine-p-nitroanilide hydrochloride, Sigma B-4875) substrate dissolved 500 µL of DMSO in 50 mM Tris-HCl buffer ...
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bioRxiv - Developmental Biology 2022Quote: Mouse primary pancreatic cells were obtained by digesting the whole pancreas with 1 mg/mL Collagenase P (Sigma, 11213865001) supplemented with 2 U/mL Dispase II (Life Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... transgenic and mutant zebrafish embryos were raised at 28°C in Embryo Water (Volvic© water containing 0.28 mg/mL Methylene Blue [M-4159; Sigma] and 0.03 mg/mL 1-phenyl-2-thiourea [P-7629; Sigma]), and staged according to Westerfield (Westerfield ...
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bioRxiv - Biochemistry 2020Quote: ... A 96-well dot blot apparatus (Schleicher & Schuell) was set up with a 0.45-μm-pore-size polyvinylidene difluoride (PVDF) membrane (Immobilon-P; Millipore), and each dot was rinsed with 500 μl of TBS ...
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bioRxiv - Molecular Biology 2020Quote: ... The samples were electrophoresed on denaturing SDS-polyacrylamide gels and transferred onto Immobilon-P PVDF membranes (Merck Millipore, USA). The membranes were then blocked using 5% non-fat milk in PBS for 30 minutes followed by overnight incubation at 4°C with primary antibody against protein of interest at the desired dilution in 2% non-fat milk prepared in PBS ...