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Citations for Millipore Sigma :
2651 - 2700 of 10000+ citations for Ribonuclease P Protein Subunit p38 RPP38 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... were subjected to protein extraction using Plant Total Protein Extraction Kit (Sigma-Aldrich). The protein concentration was measured using 2-D Quant Kit (GE Healthcare ...
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bioRxiv - Genetics 2020Quote: ... Protein concentrations were analyzed with a Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich), using bovine serum albumin as a standard ...
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bioRxiv - Immunology 2020Quote: Total proteins isolated from BMDCs were lysed in CytoBuster protein extraction reagent (Novagen). Protein quantification was performed using NanoDrop One Spectrophotometer ...
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bioRxiv - Cell Biology 2022Quote: ... the other stained for proteins with SYPRO Red Protein Gel Stain (Sigma Aldrich). Both dyes were used according to the manufacturers’ protocols.
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bioRxiv - Molecular Biology 2024Quote: The protein mixtures were filtered using a low protein binding filter (Millipore Sigma) and purified on a SourceQ15 anion exchange column (Cytiva ...
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bioRxiv - Genetics 2024Quote: ... protein concentration was measured by use of a BCA Protein Assay (SIGMA Aldrich) and samples stored at -80°C.
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bioRxiv - Genomics 2024Quote: ... Protein transfer and total protein content was assessed by Ponceau Staining (Sigma Aldrich). Ponceau Stain was washed out with 1x TBST ...
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bioRxiv - Microbiology 2022Quote: ... total cell protein was measured using the bicinchoninic acid protein assay (Sigma-Aldrich) against bovine serum albumin standards ...
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bioRxiv - Microbiology 2023Quote: ... or with slurry protein G beads (Sigma-Aldrich Fast Flow Protein G sepharose). For GFP-p65 and GFP-COMMD2 the samples were immunoprecipitated as per manufacturer’s instructions with the elution was recovered in either 5x with Laemmli buffer 74 and boiled at 98°C (dry bath ...
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bioRxiv - Microbiology 2023Quote: ... 1000 µg of protein extracts were cleared with agarose protein A beads (Millipore) at 4 ℃ for 1.5 h on a rocking platform ...
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bioRxiv - Plant Biology 2023Quote: ... the protein extract was incubated with 30 μL Protein A Agarose beads (Millipore), 10 μL anti-SIZ1 antibody with rotation at 4°C for 4 h.
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bioRxiv - Microbiology 2023Quote: ... The protein was purified using a Protein A agarose gravity column (Millipore Sigma) followed by SEC using a S200 column.
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bioRxiv - Cancer Biology 2023Quote: Oxidized proteins were detected using the OxyBlot Protein Oxidation Detection Kit (Sigma, S7150) and following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentrations were determined with the BCA protein assay kit from Sigma-Aldrich.
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was quantified using bicinchoninic acid (BCA) Protein assay (Sigma-Aldrich). Then 60 μg of the total protein of each genotype was separated on 8% SDS-PAGE and transferred to the PVDF membrane (Amersham ...
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bioRxiv - Molecular Biology 2021Quote: Protein-G (Protein G Sepharose® 4 Fast, cat# GE17-0618-01) or Protein-A (ProteinA-Sepharose® 4, cat#P9424 Millipore) beads were washed twice with 1x PBS and twice with IP100 buffer (25 mM Tris-HCl 7.9 ...
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bioRxiv - Microbiology 2021Quote: ... the following were incubated for 15-18hrs at 4°C in PBS with 0.3% Triton-X and 5% goat serum: mouse biotin conjugated anti-NeuN B-clone A60 (MAB377, Millipore, 1:500), rabbit anti-calbindin (C2724 ...
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bioRxiv - Molecular Biology 2021Quote: ... HCT116 stable cell line expressing TurboID-CLUH and TurboID-GFP were grown for 30 minutes and 16 hours in presence of 50 µM of biotin (Sigma Aldrich). About 20 million cells (two 10-cm plates ...
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bioRxiv - Molecular Biology 2020Quote: ... empty biotin modified hydrogel spheres were modified with 1:1 volume ratio of 1 mg/ml streptavidin (Sigma-Aldrich, cat #85878) for 5 min ...
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bioRxiv - Biochemistry 2020Quote: ... Heparin (20 μl of 50 mg.ml−1) was reacted with 20 μl hydroxylamine-biotin in 40 μl 300 mM aniline (Sigma-Aldrich, UK) and 40 μl 200 mM acetate pH 6 for 48 h at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... revelation was performed using an avidin/biotin complex coupled with peroxidase (ABC kit, Universal Vectastain Kit PK6200) and diaminobenzidine (Sigma Aldrich).
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bioRxiv - Microbiology 2020Quote: ... The wells were further blocked with 15 µl of 10 µM biotin in 3% skim milk in PBST (Sigma, cat# 1.15363.0500) for ten min at R.T ...
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bioRxiv - Neuroscience 2021Quote: TG2 activity of EVs either intact (resuspended in PBS) or lysed (resuspended in mild lysis buffer) was measured through incorporation of biotin cadaverine (BTC, Sigma-Aldrich) into FN as previously described (Jones ...
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bioRxiv - Cell Biology 2022Quote: ... Log phase promastigote cultures (seeded at 2×106 cell/ml) were allowed to grow in complete M199 supplemented with 150 μM biotin (B-4639, Sigma-Aldrich) for 18-24 hours ...
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An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: ... sections were washed trice with PBS and incubated for 1h at RT with Avidin-Biotin complex (Vector) and then with DAB/H2O2 mix (Sigma-Aldrich).
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bioRxiv - Biophysics 2020Quote: ... The created wells were washed three times with PBS buffer and then incubated with BSA-biotin (0.5 mg/mL, Sigma-Aldrich, USA) and NeutrAvidin (0.2 mg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... the lysate (1 mg of protein in 500 μl) was aliquoted per sample and then the following were added: 10 μl of 5 mM biotin picolylazide (900912 Sigma-Aldrich) and 50 μl of click reaction mix (three parts TBTA 5 mM TBTA in butanol:DMSO (4:1 ...
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bioRxiv - Genetics 2020Quote: ... 50 mL of blocking buffer was prepared per 2×108 cells by adding 1 gram of biotin-free BSA (Sigma Aldrich) and 200 μl of 0.5 M pH 8.0 EDTA (ThemoFisher 15575020 ...
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bioRxiv - Neuroscience 2021Quote: ... Free-floating tissue sections were incubated overnight at room temperature for 22 hours using a 1:500 dilution of biotin-labelled Wisteria floribunda agglutinin (WFA) (L1516, Sigma-Aldrich) in PBS + 0.25% Triton X-100 ...
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bioRxiv - Cell Biology 2020Quote: ... washed twice with 500 µL PBS and re-suspended in 500 µL of PBS containing ∼3 mg/mL Biotin-NHS (Sigma B1022). Cells were incubated for 30 min on a rotating wheel at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... the embryos were incubated in the biphasic solution for 30 min and 100 μl of 500 nM D-Biotin (Sigma-Aldrich) were added and mechanically mixed with the solution ...
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bioRxiv - Genomics 2022Quote: ... The reactions were rotated at 40 RPM for 30 minutes to allow for biotin-streptavidin binding and then placed on a magnetic separation rack (20-400, Sigma-Aldrich) until the solution was clear ...
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bioRxiv - Systems Biology 2022Quote: ... 50 mL of blocking buffer was prepared per 2 × 108 cells by adding 1 g of biotin-free BSA (Sigma Aldrich) and 200 mL of 0.5 M pH 8.0 EDTA into DPBS (GIBCO) ...
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bioRxiv - Molecular Biology 2022Quote: Biotinylated DNA was generating using a forward primer containing a Biotin TEG group on the 5’ end obtained from Sigma Aldrich: Biotin TEG 5’ ...
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bioRxiv - Biophysics 2022Quote: ... The non-reacted biotin was then removed by passing the biotinylated sample through a PD SpinTrap G-25 column (Sigma-Aldrich) with a molecular weight cutoff of 5000 Da ...
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bioRxiv - Neuroscience 2024Quote: ... AP-SEP-GluA2-BirAER and the Tet-on transactivatior and cultured in the presence of 10 μM biotin (Sigma-Aldrich; #B4501). 100 nM doxycycline (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: Tubulin was purified from porcine brain as described before (Castoldi and Popov, 2003) and labeled with biotin-N-hydroxysuccinimide (NHS; Sigma-Aldrich) or ATTO-647N-NHS (ATTO-TEC ...
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bioRxiv - Biochemistry 2024Quote: ... biotinylation of proteins proximal to V5-APEX2-SENP2 was performed according to previously published protocol [40] with a minor modification where Biotin-Phenol (SML2135, Sigma-Aldrich) was added to the media at a final concentration of 1 mM and the incubation with Biotin-Phenol was extended to 40 min.
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bioRxiv - Biophysics 2023Quote: ... and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) (biotin-DOPE) (structures in Table S5) were purchased from Sigma-Aldrich (UK). Agarose powder was purchased from Invitrogen (UK) ...
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bioRxiv - Microbiology 2023Quote: ... 0.1 mL vitamin solution (10 μg biotin, 10 μg cobalamin, 30 μg 4-aminobenzoic acid, 50 μg folic acid and 150 μg pyridoxamine, Sigma-Aldrich) and 6.2 mL of volatile fatty acids solution (2.11 mL acetic acid (Sigma-Aldrich) ...
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bioRxiv - Genomics 2022Quote: ... in PBS) were used for the spectrum codes preparation followed by reacting with 1 μL biotin-NHS (Sigma, 2 mg/mL) for 2 hours in room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... followed by washing with 1XPBS two times for 2’ each and blocked again with Biotin (0.001%, Sigma-Aldrich, Merck, Overijse, Belgium) for 20’ and washed twice for 2’ in 1XPBS each ...
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bioRxiv - Systems Biology 2023Quote: ... 50 mL of blocking buffer was prepared per 2 x 108 cells by adding 1 g of biotin-free BSA (Sigma Aldrich) and 200 mL of 0.5 M pH 8.0 EDTA into DPBS (Gibco) ...
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bioRxiv - Biophysics 2023Quote: ... containing 0.3□mM biotin and concentrated at +4 °C to 25 μl volume using Amicon ®Ultra 10 kDa MWCO centrifugal filters (Merck Millipore).
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bioRxiv - Cancer Biology 2023Quote: ... A total of 250 mg of Biotin (BioBasic ref# BB0078) was dissolved in 2.04 ml of NH4OH 28-30% (SIGMA ref# 221228) to produce a 500 mM solution ...
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bioRxiv - Microbiology 2023Quote: Nycodenz-purified gametocytes containing 1×108 male gametocytes from either the Rbpm1::TurboID or Rbpm1::T2A::TurboID line were incubated with 50 µM biotin (cat. no. B4639, Sigma-Aldrich) at 37°C for 20 minutes ...
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bioRxiv - Immunology 2024Quote: ... Tetramer staining was performed in combination with staining for chemokine receptors CXCR5 and CXCR6 for 1 h at RT in staining buffer supplemented with Dasatinib (50 nM) and biotin (20 nM; Sigma, B4501). After washing of the samples with FACS buffer (PBS supplemented with 1 % FCS) ...
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bioRxiv - Cell Biology 2024Quote: HT1080 cells stably expressing TurboID-CAMSAP2-S835A and TurboID-CAMSAP2-S835D or TurboID were treated with Biotin (50 µM, Sigma-Aldrich) for 10 min before harvesting ...
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bioRxiv - Genetics 2024Quote: ... GISH probes were prepared from DNA samples using a Biotin-Nick Translation Mix or a DIG-Nick Translation Mix (both from Sigma, USA). For the rDNA probe ...
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bioRxiv - Microbiology 2024Quote: ... followed with 100 µl per well of either 1:3000 diluted biotin goat anti-mouse IgG (in 0.5% skim milk PBST; Sigma SAB4600004-250µl) or 1:1000 diluted biotin goat anti-mouse IgA (BD ...