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Citations for Millipore Sigma :
2601 - 2650 of 10000+ citations for Ribonuclease P Protein Subunit p38 RPP38 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... 150 mM NaCl, 0.1% Nonidet P-40, 1% Plant Proteinase Inhibitor Cocktail [Sigma], and 50 mM 2,2′-Dithiodipyridine [Sigma]) or ...
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bioRxiv - Plant Biology 2020Quote: ... tissue was ground in lysis buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1% Nonidet P-40, 1% Plant Proteinase Inhibitor Cocktail [Sigma] ...
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bioRxiv - Immunology 2021Quote: ... and then developed at RT by addition of 100 μL of p-nitrophenyl phosphate (pNPP) substrate (Sigma-Aldrich) per well ...
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bioRxiv - Plant Biology 2020Quote: ... total proteins from 7-day-old seedlings were extracted with no-salt lysis buffer (50 mM Tris, pH 8.0, 0.1% Nonidet P-40, and 1% Protease inhibitor cocktail [Sigma]) at a fresh weight:buffer volume ratio of 1 g:2 mL ...
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bioRxiv - Bioengineering 2021Quote: ... formulation S-50 or P-0 was dissolved in 0.01 M PBS containing 50 mM citrate (Sigma-Aldrich) and 20 mM EDTA (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2021Quote: ... 20 μg of RBD samples were applied to the hydrophobic Immobilon-P PVDF membrane (Millipore, Amsterdam, The Netherlands) in a 96-well plate format ...
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bioRxiv - Microbiology 2020Quote: ... Soils were amended with 150 µl of either 13C7-labeled p-hydroxybenzoic acid (99% atom 13C; Sigma-Aldrich) or unlabeled control (‘12C’ ...
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bioRxiv - Neuroscience 2020Quote: ... the lysates were separated on SDS-polyacrylamide gels and transferred to polyvinylidene fluoride membranes (Immobilon P membrane, Millipore). The membranes were blocked at room temperature for 30 min in TBST containing 5% skimmed milk (Nacalai Tesque ...
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bioRxiv - Cancer Biology 2021Quote: ... and then transferred to an activated Immobilon-P PVDF Membrane (Merck Millipore Ltd, IPVH00010 PORE SIZE: 0.45 µm). The membranes were blocked using 5% nonfat dry milk in PBS/0.1% Tween20 for 1h at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... nDKOLuc and nTKOLuc mice between 1-5 months were intraperitoneally (i. p.) injected with tamoxifen (Sigma-Aldrich, T5648) at 1mg in 100µl corn oil for 5 consecutive days to induce Cre activity and tumorigenesis ...
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bioRxiv - Plant Biology 2021Quote: ... the extracts were mixed with the same volume of fresh Ehrlich’s reagent [0.2 g p-dimethylaminobenzaldehyde (Sigma-Aldrich), 8.4 mL acetic acid ...
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bioRxiv - Bioengineering 2020Quote: ... 20 mM L-Glutamine and 1% penicillin (100 U/ml) and streptomycin (100 μg/ml) (P/S) (Sigma). MSCs were cultured in Roswell Park Memorial Institute (RPMI-1640 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... supplemented with 10% Foetal Bovine Serum (FBS; HyClone, SH3007103) and 1% Penicillin-Streptomycin (P/S; Sigma Aldrich, P4333). Cells were incubated at 37°C with 5% CO2 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... samples (90 μl) were derivatized for 3 min with 10 μl of 60% p-toluenesulfonyl isocyanate (PTSI) (Sigma) in acetonitrile (Hu et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Phospho-tyrosine immunoprecipitations were conducted with anti phosphotyrosine (anti p-Tyr) agarose-coupled beads clone 4G10 (Millipore Sigma) and sepharose-coupled beads P-Tyr-1000 (Cell Signaling Technology) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... we treated the bees on Day 1 with the allatoxin Precocene-I (“P-I”; Sigma-Aldrich, cat # 195855) suspended in castor oil vehicle solution ...
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bioRxiv - Cell Biology 2020Quote: ... 50 U/ml penicillin and 50 μg/ml streptomycin (P/S) (all from Sigma-Aldrich, St. Louis, MO). Non-essential amino acids (PAN-Biotech ...
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bioRxiv - Molecular Biology 2020Quote: ... The samples were separated by SDS-PAGE and electrotransferred to a 0.45 μm Immobilon-P PVDF membrane (Millipore). The membrane was incubated overnight at 4°C with DCP5 antibodies (provided by Rémy Merret and Cécile Bousquet-Antonelli ...
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bioRxiv - Immunology 2021Quote: ... Histopaque solution (Cat no.- 10771-100ml) and poly-l-lysine (Cat no.- P-8920) were purchased from Sigma. TMB solution (Cat no.- CL07-1000MLCN ...
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bioRxiv - Biophysics 2021Quote: ... The Outer Aqueous (OA) solution additionally contained 50 mg mL−1 of Kolliphor P-188 (Sigma-Aldrich, UK) as per standard OLA protocols.38 Lipids were purchased from Sigma Aldrich in powder form and were dissolved in 100% ethanol to a final concentration of 100 mg mL−1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and KDEL) or 10% (GAPDH) SDS□PAGE and transferred to an Immobilon-P transfer membrane (Millipore, Billerica, MA). For PXR ...
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bioRxiv - Immunology 2023Quote: ... the plates were washed 3 times and the reaction was developed with 100uL p-Nitrophenyl phosphate (PNPP, Sigma; N-9389 ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples from exponentially growing cells were separated on a SDS–polyacrylamide (37.5:1) gel electrophoresis and blotted on 0.45µm pore PolyVinyliDenFluoride (PVDF) membranes (Immobilon-P from Sigma Aldrich). Membranes were blocked for 2 hours with 1x Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2023Quote: ... SDS-protein samples were separated by electrophoresis using a handmade SDS-polyacrylamide gel (7.5 or 9% polyacrylamide) and transferred to a PVDF membrane (Immobilon-P, pore size 0.45 µm; Merck-Millipore). The membrane was probed with primary antibodies listed in Supplemental Table 3.
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bioRxiv - Cell Biology 2023Quote: ... and 100 U/mL penicillin and 100 μg/mL streptomycin (P/S, Sigma-Aldrich, Saint Louis, MO, USA). Caco-2 cells were stimulated with 60 μg/mL of lipopolysaccharide (LPS ...
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bioRxiv - Molecular Biology 2023Quote: ... Mammalian expression construct for FLAG-VCF1 was generated by inserting VCF1 cDNA into p-FLAG-CMV-2 (Sigma). Point mutations were introduced using the Q5 Site-Directed Mutagenesis kit (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we pipetted an aliquot of 20μl into 50μl of a phosphate buffered saline solution (PBS; Sigma P-4417) containing 1% formalin.
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bioRxiv - Cell Biology 2023Quote: ... Protein samples (5 to 25µg/lane) were separated by 12.5% – 15% SDS-PAGE and transferred onto Immobilon-P PVDF membranes (Merck Millipore). Proteins were probed in Can Get Signal Solution1 (Toyobo ...
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bioRxiv - Cancer Biology 2024Quote: Cell were lysed in RIPA buffer (1X PBS (137mM NaCl; 2.7mM KCl; 4.3mM Na2PO4; 1.47 mM KH2PO4) with 1% Nonidet P-40 Substitute (Sigma #74385), 0.5% Sodium deoxycholate ...
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bioRxiv - Molecular Biology 2023Quote: Procyclic promastigotes of Leishmania major strain LV39 (MRHO/SU/59/P) were cultivated in M199 medium (Sigma Aldrich) supplemented with 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Plant Biology 2024Quote: ... The samples were separated by SDS-PAGE and transferred to a 0.45 μm Immobilon-P PVDF membrane (Millipore). The membrane was incubated 2h at 4°C with ANTI-FLAG antibodies® M2-peroxydase (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... resolved on 7.5 or 12% SDS-PAGE gels and transferred onto PVDF membranes (Immobilon-P PVDF Transfer Membrane 0.45, Millipore). Membranes were blocked using 5% BSA or 5% non-fat dried milk in TBS containing 0.05% Tween 20 (Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: ... supplemented with 10% fetal calf serum (FCS, Capricorn, FBS-12A) and 1% penicillin/streptomycin (P/S, Sigma, P0781). The cultured fibroblasts were incubated at 37°C with 5% CO2.
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bioRxiv - Bioengineering 2022Quote: ... 200 mmol/L of L-glutamine (L-G) and 1% (vol/vol) penicillin-streptomycin (P/S; Sigma, US) (standard culture medium) ...
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bioRxiv - Neuroscience 2022Quote: ... washed again three times in TBSt and signal was developed with p-nitrophenyl phosphate (pNPP Sigma Cat #N2765), the color reaction being quantified at 405nm.
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bioRxiv - Neuroscience 2022Quote: ... we delivered the Iκ-kinase inhibitor [5-(p-Fluorophenyl)-2-ureido]thiophene-3-carboxamide (TPCA-1; Sigma-Aldrich CAS 507475-17-4 ...
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bioRxiv - Microbiology 2023Quote: ... P cells were grown till 0.5 OD600 in 2 ml LB with 1 mM deferoxamine mesylate (DFO, Sigma) or 1 mM FeCl3 respectively ...
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bioRxiv - Cell Biology 2024Quote: Cells were lysed on ice in NP-40 lysis buffer (0.5% Nonidet P 40 Substitute (74385, Sigma-Aldrich), 25 mM Tris–HCl ...
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bioRxiv - Immunology 2024Quote: ... PBMC were incubated in the presence of 10 μg/ml PHA-p (Sigma Aldrich Corporation, St. Louis, MO) and 100 IU/ml hIL-2 (PeproTech ...
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bioRxiv - Microbiology 2024Quote: The hydrolytic activity of the bacterial DPP4 enzyme was measured using Gly-Pro p-nitroanilide hydrochloride (gpPNA) (Sigma), as described for assessing hsDPP4 activity [39] ...
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bioRxiv - Cell Biology 2024Quote: ... and osteoblast-specific alkaline phosphatase activity was measured at 405 nm by using 120 mM p-nitrophenolphosphate (Sigma) in 0.1 M Tris buffer (pH 8.5) ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were separated by SDS-PAGE and transferred to Immobilon-P polyvinylidene difluoride membranes (IPVH00010; EMD Millipore). Immunoblotting analysis was performed with the indicated antibodies ...
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bioRxiv - Immunology 2024Quote: ... microplates were washed 4 times and 25 µl of p-nitrophenyl phosphate (pNPP) substrate (Sigma, USA, Cat#N7653) at 1 mg/ml in carbonate buffer at pH 9.6 was added and further incubated for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.1% SDS) at room temperature for 20 min and then transferred to PVDF membranes (Immobilon-P, Millipore). Membranes were probed with anti-HA or anti-DYKDDDDK antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were separated by SDS-PAGE and transferred to Immobilon®-P PVDF Membrane (0.45mm pore size; Millipore) per standard protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... Glycoproteins were dot blotted (two dots each) onto a PVDF membrane (Immobilon P, 0.22 µm pore, Merck Millipore), and N- and O-glycans were sequentially released ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were separated on SDS-PAGE gels and transferred to Immobilon-P transfer membranes (cat# IPVH00010, Merck Millipore). The following antibodies were used to probe the membranes ...
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bioRxiv - Molecular Biology 2020Quote: ... protein was isolated from cells using 300μl CytoBuster™ Protein Extraction Reagent (Novagen) per well according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentrations were determined with the BCA protein assay kit (Novagen, Merck KGaA), and protein homogeneity was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extracts were performed with the Plant Total Protein Extraction Kit (Sigma, USA). Protein concentrations were measured using the BCA method ...