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Citations for Millipore Sigma :
2551 - 2600 of 10000+ citations for Ribonuclease P Protein Subunit p38 RPP38 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cells were washed three times with ice-cold PBS and incubated with 0.25 µg/mL biotin hydrazide (66640-86-6, Sigma-Aldrich) in cold PBS for 1 hour on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed with PBS and media was changed to 10% FBS DMEM supplemented with 500 µM biotin (Sigma Aldrich) in DMSO ...
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bioRxiv - Molecular Biology 2023Quote: T7-driven pET-42a(+) construct containing SPUD transcript was used for in vitro transcription using RNA Biotin Labelling Mix (Sigma) and T7 polymerase (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... Myoblasts were grown in 15 cm culture dishes at sub confluency and incubated with biotin (Sigma-Aldrich: dissolved in DMSO) at a final concentration of 50 uM for 18 h ...
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bioRxiv - Biochemistry 2023Quote: ... Azo biotin-azide (CAS No. 1339202-33-3) and Imatinib (Cas No.152459-95-5) were obtained from Sigma Aldrich, rapamycin (Cas No.53123-88-9 ...
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bioRxiv - Neuroscience 2023Quote: ... samples were washed 3 times for 15 min in PBS and incubated in 0.0001% biotin-tyramide (Sigma Aldrich, SML2135-50MG) and 0.003 % H2O2 in 0.05 M borate buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then biotinylated for 10-12 min in E4 containing 1.25 mg/mL NHS-SS-Biotin (Pierce) and quenched in E4 containing 20mM glycine (Sigma-Aldrich). Cells were then scrapped in cold PBS supplemented with protease inhibitors (Protease inhibitor cocktail set III ...
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bioRxiv - Molecular Biology 2024Quote: ... Excess biotin and DTT was removed by 6 x sequential dilution and concentration using 3KDa Amicon Ultra centrifugal filter (Millipore). Yield of biotin conjugation was tested by intact MS with an Agilent 6230 b LC/MS TOF mass spectrometer ...
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bioRxiv - Microbiology 2023Quote: ... Cyst walls were stained with a 1/300 dilution of a biotin-labelled Dolichos biflorus lectin (L-6533, Sigma-Aldrich) for 1h and revealed using a 1/300 dilution of FITC-conjugated streptavidin (SNN1008 ...
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bioRxiv - Microbiology 2023Quote: ... from a 50 mM biotin stock solution prepared in water with 60 mM NaOH (lyophilized biotin powder, 500 mg stock, Merck Life Science BV (Sigma)) ...
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bioRxiv - Molecular Biology 2023Quote: ... pstv6-NBirA*-FLAG-TRIP12 cells were incubated with 1 µg/mL of doxycycline and 50 µM of biotin (Sigma-Aldrich) for 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... CTPI-2 (ENA018104423), puromycin (P8833), doxycycline (D9891), biotin (B4639) trichostatin A (TSA, T8552) and nicotinamide (NAM, N0636) were purchased from Sigma. NDI-091143 was purchased from Aobious (AOB17806) ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining biotin on the cell surface was stripped with 50 mM of sodium 2-mercaptoethanesulphonate (MESNa) (M1511, Sigma-Aldrich) in PBS/CM at 4°C for 30 min followed by quenching with 5 mg/ml iodoacetamide (093-02152 ...
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bioRxiv - Bioengineering 2023Quote: ... Precusor nCys- mEpoR peptide was labeled at its free amino N-terminus as a protected peptide on resin with NHS-Biotin (Sigma) by swelling the resin with DMF having 10 equivalents N,N- Diisopropylethylamine ...
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bioRxiv - Genomics 2023Quote: ... 1ug gDNA was suspended in 95uL DNA elution buffer with 5uL 20 mM DBCO-PEG4-biotin (DMSO solution, Sigma, 760749), 25 mM K3BO3 while being gently shaken at 37°C for 1.5 hours ...
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bioRxiv - Immunology 2024Quote: The valency of purified monovalent and tetravalent Strep-Tactin SpyCatcher was measured using biotin-4-fluorescein (Sigma-Aldrich #B9431-5MG) which when bound to Strep-Tactin become quenched.
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bioRxiv - Microbiology 2024Quote: ... we washed the cells thrice with DPBS and added fresh infection medium containing 1 mM biotin-phenol (Sigma Aldrich, SML2135) as well as amitriptyline and ionomycin ...
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bioRxiv - Biochemistry 2024Quote: ... thawed GST-BirA enzyme (20 µL of 50 µM solution) and D-Biotin (3 µL of 50 mM solution in 100 % DMSO; Sigma) and incubated for 1 h minimum at 30 °C in an incubator with gentle shaking (90 rpm) ...
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bioRxiv - Neuroscience 2020Quote: ... Tau protein ladder (Sigma) was used in some experiments as a control ...
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bioRxiv - Biophysics 2022Quote: ... BLUeye protein ladder (Sigma) was used as a standard ...
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bioRxiv - Biophysics 2022Quote: ... standard proteins BSA (Sigma), Apoferritin (Sigma ...
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bioRxiv - Plant Biology 2022Quote: Protein A-sepharose (Sigma) was washed twice with 5 column volumes of buffer HNTG [20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Protein disulfide isomerase (Sigma) was used in positive control reactions and albumin (Research Organics ...
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bioRxiv - Immunology 2021Quote: ... plus protein A (Sigma).
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bioRxiv - Biochemistry 2024Quote: ... S protein agarose (Millipore) was added to each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... ECL protein detection (Millipore) was performed with a Fusion Fx7 chemiluminescent imager ...
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bioRxiv - Immunology 2024Quote: ... protein G beads (Millipore) were used (2 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Protein-A agarose (Sigma), Streptavidin agarose (ThermoFisher) ...
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bioRxiv - Biochemistry 2020Quote: ... The precipitated proteins were used for determination of protein content using a Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich, Taufkirchen, Germany).
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bioRxiv - Microbiology 2022Quote: ... The SPA-tagged Rho protein was visualized by hybridization with the primary mouse ANTI-FLAG M2 monoclonal antibodies (Sigma-Aldrich; dilution 1:5,000) and the secondary goat peroxidise-coupled anti-mouse IgG antibodies A2304 (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: For profiling the wildtype and oncogenic KMT2A protein we used two monoclonal antibodies targeting the KMT2A N-terminus: Mouse anti-KMT2A (1:100, Millipore Cat #05-764) refered to as KMT2A-N1 ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Neuroscience 2020Quote: Primary antibodies used for immunofluorescence on whole-mounted retinal sections were brain-specific homeobox/POU domain protein 3A (Brn3a; Millipore; cat #MAB1585; RRID:AB_94166) at 1:1000 and GFAP (1:1000) ...
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bioRxiv - Cell Biology 2021Quote: ... and screened for expression of the fusion proteins by western blotting with an anti-FLAG M2-HRP antibody (Sigma, mouse monoclonal, cat#: A8592).
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1 µg/ml of a SARS-CoV nucleocapsid protein (NP) monoclonal antibody (mAb) 1C7C7 (Millipore-Sigma, St. Louis, MO) shown to cross-react with SARS-CoV-2 NP ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight while shaking at 4°C in the blocking solution that contained combinations of the following primary antibodies: mouse anti- three microtubule-binding domain tau protein (3R-tau, 1:500, Millipore, Cat# 05-803), rabbit anti-purkinje cell protein 4 (PCP4 ...
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bioRxiv - Biophysics 2020Quote: ... 20 μL of either eIF4GI or DAP5 capture antibodies were incubated with PureProteome™ Protein A/G mix magnetic Beads (EMD Millipore Corporation) at room temperature with continuous mixing for 1 hr ...
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bioRxiv - Biochemistry 2021Quote: ... 25 µg of total protein were separated by denaturing PAGE and subjected to Western blotting using mouse monoclonal anti-FLAG antibody (M2, Sigma Aldrich, 1:1000) followed by probing with an HRP-coupled anti-mouse light chain-specific secondary antibody (1:10000 ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1 µg/ml of a SARS-CoV nucleocapsid protein (NP) monoclonal antibody (mAb) 1C7C7 (Millipore-Sigma, St. Louis, MO) shown to cross-react with SARS-CoV-2 NP ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding to N protein was detected using conventional two-step ELISA with an HRPO-conjugated anti-mouse or anti-rat Fc antibody (Sigma Aldrich cat # A9309). All assays included a standard curve of mouse neutralising S1 antibody and mouse anti-N antibody (Sino Biological) ...
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bioRxiv - Neuroscience 2023Quote: Whole retinas were flat-mounted on slides coated with Gatenby’s solution and stained sequentially using the following primary antibodies: brain-specific homeobox/POU domain protein 3A (Brn3a; Millipore; cat #MAB1585; RRID: AB_94166) at 1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... separate sets of sections were immunohistochemically processed using anti-glial fibrillary acidic protein (GFAP) monoclonal antibody 1:500 (G3893, Sigma, Saint Louis, Missouri), anti-doublecortin (DCX ...
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bioRxiv - Physiology 2023Quote: ... We blocked sections in 5% normal goat serum in phosphate-buffered saline with 0.1% Tween 20 (blocking buffer) for 30 min at room temperature (RT) and then incubated in primary antibody for laminin protein (1:500, Sigma catalog no. L93931) overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight while shaking at 4◦C in the blocking solution that contained combinations of the following primary antibodies: mouse anti-3 microtubule-binding domain tau protein (3R-Tau, 1:500, Millipore, Cat# 05-803), rabbit anti-Purkinje cell protein 4 (PCP4 ...
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bioRxiv - Neuroscience 2022Quote: ... The immunoprecipitated proteins were analyzed by SDS-PAGE and Western blotting using the following antibodies: anti-HA (Sigma, H3663; clone 12CA5, 1:1000), anti-Flag (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... Puromycin incorporated into the proteins were detected in a Western blot using a mouse-origin anti-puromycin monoclonal antibody (12D10, Sigma MABE343, lot # 3484967) and a goat-anti-mouse HRP-conjugated secondary antibody (Abcam ab6789 ...
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bioRxiv - Molecular Biology 2024Quote: ... Equal protein loading was verified by the detection of β-Actin using the A3854 Monoclonal Anti-β-Actin−Peroxidase antibody (Sigma, 1:10000).
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 3.5 h in complete IMDM medium containing 0.1 μM phorbol 12-myristate 13-acetate (P-8139 Sigma) and 1 μM ionomycin (I-0634 Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were separated on SDS-PAGE gels and transferred to Immobilon-P transfer membranes (cat# IPVH00010, Merck Millipore). The following antibodies were used to probe the membranes ...
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bioRxiv - Plant Biology 2020Quote: ... pH 7.5, 150 mM NaCl, 1mM EDTA, 0.1% Nonidet P-40, 10% glycerol, 1% Plant Proteinase Inhibitor Cocktail [Sigma] ...