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Citations for Millipore Sigma :
2951 - 3000 of 10000+ citations for Ribonuclease P Protein Subunit p38 RPP38 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Samples were separated by SDS-PAGE on a 12% (vol/vol) acrylamide gel and transferred to polyvinylidene difluoride (PVDF) Immobilon-P membranes (Millipore). Primary antibodies specific for human IL-18 (MLB International PM014) ...
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bioRxiv - Cell Biology 2023Quote: ... Ca2+ imaging and NAD(P)H imaging Jurkat cells were untreated or treated with 100 ng/ml of Nocodazole (Sigma) for 18 h for mitotic synchronization.
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bioRxiv - Bioengineering 2022Quote: ... 3 mM aniline dimer solution was freshly prepared by dissolving 5.5 mg of N-phenyl-p-phenylenediamine (Sigma-Aldrich, 241393) in 10 mL of Tyrode’s solution ∼20 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... or 50 uL of supernatant (for NIH 3T3) with 100 ul of 2 mg/mL (in 50 mM Tris, pH 7.6) leucine p-nitroanilide (LpNA; Sigma, L9125). Samples were incubated at 37 ° ...
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bioRxiv - Cell Biology 2022Quote: ... Equal volumes of deproteinized sample were mixed with a freshly made 20 mg/ml solution of p-dimethyl-amino-benzaldehyde (Ehrlich’s reagent; Sigma # 109762) in glacial acetic acid at room temperature to allow for the formation of a yellow colored product ...
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bioRxiv - Biochemistry 2022Quote: ... The concentration of product was calculated by linear regression equation given on the standard curve performed by the reference compound p-nitrophenol (Sigma). One unit of enzyme activity was defined as 1 μmol of p-nitrophenol produced per minute under the assay conditions.
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bioRxiv - Biochemistry 2023Quote: ... 1% Nonidet P-40) in the presence of a protease inhibitor cocktail and phenylmethanesulfonyl fluoride (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Neuroscience 2023Quote: ... Forty micrograms protein/lane was electrophoretically separated on a 10% SDS polyacrylamide gel and electrically transferred onto a Hybond-P PVDF transfer membrane (Millipore) for 1.5 h ...
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bioRxiv - Cell Biology 2024Quote: ... The sample was washed twice with 0.1 % BSA-PBS and covered with mounting medium [1 mg/mL p-phenylenediamine (Sigma-Aldrich) in a mixture of 90% glycerol and PBS] and 24 mm × 60 mm coverslip (Matsunami Glass IND. ...
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bioRxiv - Cancer Biology 2024Quote: ... samples of the whole cell extract and immunoprecipitates were separated by SDS-PAGE and transferred onto 0.45 μm Immobilon-P PVDF membranes (Millipore Sigma). Membranes were then blocked in 5% nonfat dried milk / PBST for 30 minutes at room temperature and incubated with the indicated primary antibodies at 4°C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... for 3 hours at 15° C following addition of 10 µM 9:1 S-adenosyl-L-methionine (SAM) p-toluenesulfonate salt (Sigma) to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM ...
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bioRxiv - Biochemistry 2024Quote: ... for 30 minutes at 30° C following addition of 10 µM 9:1 S-adenosyl-L-methionine (SAM) p-toluenesulfonate salt (Sigma) to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM ...
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bioRxiv - Molecular Biology 2024Quote: ... 786-O, 769-P, A704, and RCC- FG2 (Cell Lines Service, CLS GmbH) cells were cultured in RPMI 1640 medium (Sigma) supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Biochemistry 2024Quote: Cell lysates were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions and transferred to polyvinylidene difluoride membranes (Immobilon P, IPVH00010, Millipore). Primary antibodies used were anti-HA High Affinity ...
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bioRxiv - Biochemistry 2024Quote: ... for 1 hour at 30° C following addition of 10 µM 9:1 S-adenosyl-L-methionine (SAM) p-toluenesulfonate salt (Sigma) to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM ...
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bioRxiv - Plant Biology 2024Quote: ... for 5 minutes and the supernatant was separated on a 12% SDS-PAGE gel and subsequently transferred onto a PVDF membrane (Immobilon-P, Millipore). Immunodetection of the proteins was performed according to standard protocols using the antibodies detailed (Table 3) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were mounted in 10 µL of mounting medium (80% glycerol, 0.1 M Tris pH 8.8, 0.05% p-phenylenediamine dihydrochloride (Sigma, cat. no. P1519)) and stored at −20°C.
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bioRxiv - Genetics 2024Quote: ... and incubated for 1 hour at 30°C following addition of 10 µM 9:1 S-adenosyl-L-methionine (SAM) p-toluenesulfonate salt (Sigma) to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM ...
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bioRxiv - Neuroscience 2024Quote: ProBDNF digestion samples were run on 15% SDS-PAGE gels and electro-transferred onto polyvinylidene difluoride membrane (Immobilon-P, Millipore), using semi-dry method ...
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bioRxiv - Biochemistry 2024Quote: ... for 1 hour at 30° C following addition of 10 µM 9:1 S-adenosyl-L-methionine (SAM) p-toluenesulfonate salt (Sigma) to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM ...
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bioRxiv - Biochemistry 2024Quote: ... for 1 hour at 30° C following addition of 10 µM 9:1 S-adenosyl-L-methionine (SAM) p-toluenesulfonate salt (Sigma) to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM ...
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bioRxiv - Physiology 2024Quote: ... the pancreas was perfused via the pancreatic duct using an ice-cold collagenase solution (Collagenase P, 11213865001, Merck/Sigma Aldrich) with a concentration of 0.63 mg/ml ...
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bioRxiv - Microbiology 2024Quote: Soluble cell extracts were separated by SDS-PAGE electrophoresis (10%) and later Coomassie blue-stained and/or electroblotted onto a PVDF Inmobilon-P membrane (Millipore). To specifically detect TpiAET ...
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bioRxiv - Immunology 2024Quote: ... AP activity was determined by adding 100 μl of substrate for AP (SIGMAFAST™ p-nitrophenyl phosphate tablets, Sigma-Aldrich) during ∼15 min ...
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bioRxiv - Immunology 2024Quote: Cell lysates were subjected to SDS-PAGE (50 μg unless indicated otherwise) and transferred onto an Immobilon-P polyvinylidene difluoride membrane (PVDF; Millipore). After blocking the unoccupied sites with 5% non-fat milk diluted in Tris-Buffered Saline plus Tween 20 (TBS-T) ...
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bioRxiv - Molecular Biology 2024Quote: ... The cell pellet was resuspended in 10 ml P-buffer and incubated with 1 mM PMSF (Serva) and 25 U benzonase (Novagen) on ice for 20 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... Prior to inoculating 100 L of 2X YT + P culture media supplemented with 5 mL of antifoam 204 (Sigma, A8311) in an IF 150L (New Brunswick Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... 1% v/v P/S, 100 mM sodium pyruvate (Fisher, cat#SH3023901),10-7 M dexamethosone (Sigma, cat#D4902-100mg), L-ascorbic acid phosphate (Wako USA ...
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bioRxiv - Bioengineering 2024Quote: ... phosphate-buffered saline (PBS) (1x dPBS) and 6-(p-Toluidino)-2-naphthalenesulfonic acid (TNS) were purchased from Sigma-Aldrich (Germany). mRNA encoding for firefly luciferase (CleanCap® FLuc mRNA (5moU) ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were lysed in lysis buffer (40 mM HEPES, pH 7.5, 150 mM NaCl, 0.2% Nonidet P-40, benzonase (Sigma-Aldrich, E1014) and 1× cOmplete protease inhibitor cocktail (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of protein were separated in 10-15 % SDS-PAGE under reducing conditions and transferred onto a polyvinylidene fluoride (PVDF) membrane (Inmobilon-P, Millipore) using Mini Trans-Blot Cell System (Bio-Rad Laboratories) ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were terminated by spotting 15 μL onto phosphocellulose paper (SVI-P; SVI, Melbourne, Australia) and washing extensively in 1% phosphoric acid (Sigma) and dried ...
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bioRxiv - Biochemistry 2024Quote: ... were performed from 20 15-cm plates lysed in lysis buffer (40 mM HEPES, pH 7.5, 150 mM NaCl, 0.2% Nonidet P-40, benzonase (Sigma-Aldrich, E1014) and 1× cOmplete protease inhibitor cocktail (Roche ...
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bioRxiv - Developmental Biology 2024Quote: 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, D-404S) was obtained from AccuStandard and solubilized in dimethyl sulfoxide (DMSO, D8418, Sigma-Aldrich). 17β estradiol was purchased from Sigma-Aldrich (3301 ...
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bioRxiv - Developmental Biology 2024Quote: ... The fins were then washed again with DPBS and then digested for 20 min in Collagenase P solution (1mg/ml, Merck Millipore) in Leibovitz’s L-15 medium ...
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bioRxiv - Bioengineering 2022Quote: A 10 mg mL-1 solution of Matrigel was conjugated with 20x molar excess concentration NHS-dPEG-biotin (QBD10200-50MG, Sigma-Aldrich, MO, USA) by mixing overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: Confluent primary mouse keratinocytes in 10-cm petri dishes were washed twice with ice-cold PBS followed by incubation with 8 ml of Biotin-NHS (Sigma, cat. no. 203112) in PBS (0.25 mg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated in biotinylated anti-rabbit secondary antibody 1:500 (Vectors Laboratories, #BA-1000) followed by Avidin/Biotin complex amplification (Vectastain Elite ABC kit; Vectors Laboratories, #PK-6100) and DAB (Sigma Life Science, #5905) mixed with 3% hydrogen peroxide for signal amplification ...
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bioRxiv - Neuroscience 2024Quote: Biotinylated proteins were eluted from streptavidin beads by the addition of 20 μL of elution buffer (2X Laemmli sample buffer [Bio-Rad], 20 mM dithiothreitol [Sigma-Aldrich], and 2 mM biotin [Sigma-Aldrich]) followed by a 10 min incubation at 95°C ...
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bioRxiv - Microbiology 2023Quote: Highly synchronized ring stage parasite cultures of line MD3-pfama1-GFP-BirA and Percoll-purified immature gametocyte cultures of the MD3-pffnpa-GFP-BirA parasite line as well as WT NF54 as control were treated with biotin (Sigma-Aldrich; Taufkirchen, DE), at a final concentration of 50 µM for 24 h to induce the biotinylation of proximal proteins by the BirA ligase ...
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bioRxiv - Immunology 2024Quote: ... an Amicon 3kDa molecular weight cutoff centrifugal filter column was used to remove excess biotin and the top fraction was recovered (Millipore Sigma, Cat#UFC500396). Next ...
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bioRxiv - Microbiology 2024Quote: ... 1 ml per well HBSS containing 1 mM biotin phenol was applied and biotinylation was initiated by adding 0.003% (v/v) H2O2 (Sigma Aldrich, Cat. No. H1009) for 1 min ...
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bioRxiv - Microbiology 2024Quote: 1.0×108 purified ookinetes from the gcβ::TurboID::6HA and gcβ::T2A::TurboID::6HA parasites were incubated with 50 µM biotin (Sigma-Aldrich, cat#B4639) at 22 °C for 3 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... Immunoprecipitation was performed by incubating cleared samples with 20 ul of protein G magnetic beads and anti-FLAG primary antibody (5ug, Sigma Aldrich, #F1804, raised in mouse, monoclonal) or normal mouse IgG ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein-protein interactions were assessed using the PLA Duolink in situ starter kit (Sigma Aldrich, DUO92101) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration was determined using the Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich Cat# BCA1) as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Protein-protein interactions were detected using the Detection Kit Red (DUO92008) according to the manufacturer’s (Sigma) instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and 100 µg protein was incubated with 20 µl packed protein G-sepharose beads (Sigma P3296) at 4°C for 1 hr for preclearing ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration of the collagen was verified by both BCA Protein assay (Sigma-Aldrich, cat: BCA1) and drying via vacuum centrifugation.