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Citations for Millipore Sigma :
2751 - 2800 of 10000+ citations for 6 Hydroxy 2 4 5 triaminopyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 6 mL methanol (99.8%, Sigma-Aldrich), and 8 mL UPW by gravity ...
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bioRxiv - Developmental Biology 2023Quote: ... Retinol (10-6 M, Sigma-Aldrich) was added in all organotypic cultures from D2 and then every 8 days in order to respect the meiosis entry cycle of spermatogonia (5) ...
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bioRxiv - Physiology 2024Quote: ... 6-9 DBP (Sigma-Aldrich, MO) dosage (100 ...
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bioRxiv - Immunology 2024Quote: ... 6 mM MgCl2 (Sigma-Aldrich, #M1028), 1 M betaine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... mouse IL-6 (Sigma-Aldrich, RAB0308), mouse TNF-α (Cayman ...
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bioRxiv - Cell Biology 2023Quote: ... or 6 µL PNGaseF (11365177001, Sigma) and diluted with Lysis buffer to 50 µL ...
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bioRxiv - Immunology 2023Quote: ... 6% Ficoll PM-400 (Sigma-Aldrich), 0.2% Sarkosyl (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... pH 6 (Sigma-Aldrich, C-1909) and incubated at high pressure for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... and 6 μM SB431542 (#S4317, Sigma) were added on day 2 for 24–36 h ...
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bioRxiv - Neuroscience 2023Quote: ... clone GS-6 (Millipore-Sigma #MAB302) was used at 1 ...
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bioRxiv - Neuroscience 2023Quote: ... clone GS-6 (Millipore-Sigma #MAB302) was used at 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... 6 ng of trypsin (Sigma-Aldrich) was added and the samples were incubated overnight at 37 °C ...
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bioRxiv - Plant Biology 2024Quote: ... and 10mM 6-benzylaminopurine (BAP; Sigma) were prepared in dimethyl sulfoxide (DMSO ...
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bioRxiv - Plant Biology 2019Quote: ... AGM was supplemented with vitamins (Duchefa M0409), cytokinin (200 nM 6-Benzylaminopurine), and IAA (3-indole acetic acid, 1 mM) and/or Trichostatin A (TSA, Sigma, T8552, final concentration 5 µM) or mock before pouring ...
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bioRxiv - Neuroscience 2021Quote: ... sample from the overnight cleavage step was concentrated to ∼5 mg/ml and injected into the Superose 6 Increase column equilibrated in SEC buffer with 1 mM DEET (Sigma Aldrich, CAS#134-62-3). Peak fractions were concentrated to ∼5 mg/ml and used immediately for grid preparation.
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bioRxiv - Genomics 2021Quote: HEK293T cells (ATCC® CRL-3216™) were seeded at a density of 5×105 cells/well in a 6-well culture plate (Sigma Aldrich, cat # CLS3516). The following day ...
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bioRxiv - Neuroscience 2024Quote: Sensitivity to cold was assessed immediately following testing for mechanical paw withdrawal thresholds by applying a droplet of acetone (Sigma-Aldrich; approximately 5-6 μL) from the end of a blunt one C.C ...
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bioRxiv - Cell Biology 2022Quote: ... FAM76B knockdown U937 cells were plated in 6-well plates (5×105 cells/well) and treated with 10 ng/ml PMA (Sigma-Aldrich, St. Louis, Missouri, USA) in association with 10 ng/ml LPS and 20 ng/ml hIFNγ (Sino Biological Inc. ...
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bioRxiv - Biophysics 2021Quote: ... The enzyme/protectant mixture was added 1:10 to assay buffer (100 mM Sodium Phosphate, 2 mM Sodium Pyruvate [Sigma-Aldrich], 1 mM NADH [Sigma-Aldrich], pH 6). Enzyme kinetics were measured by NAD+ absorbance at 340 nm ...
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bioRxiv - Bioengineering 2022Quote: ... electrodes were rinsed and placed in a 10 mM 6-mercapto-1-hexanol (6-MCH, Sigma-Aldrich) for 5 hours to passivate ...
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bioRxiv - Neuroscience 2021Quote: ... We then prepared a solution of 10 mg/mL 6-hydroxydopamine (6-OHDA; Sigma-Aldrich, H116-5MG) and 0.2% ascorbic acid in saline (0.9% NaCL ...
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bioRxiv - Neuroscience 2024Quote: ... We additionally prepared a solution of 10 mg/mL 6-hydroxydopamine (6-OHDA; Sigma-Aldrich, H116-5MG) and 0.2% ascorbic acid in saline (0.9% NaCL ...
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bioRxiv - Biochemistry 2020Quote: ... 2-2-2 trifluoroethanol (TFE) (Sigma, #T63002), and 200 mM dithiothreitol (DTT ...
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bioRxiv - Biophysics 2021Quote: ... Cells were then collected and re-plated at 500 cells/well with either 5 μM 4-nitroquinolone N-oxide (4NQO) (Sigma, N8141) or a dimethyl sulfoxide (DMSO ...
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bioRxiv - Microbiology 2020Quote: ... HPLC analysis was performed using a Waters e2695 separation module (Waters) equipped with a LiChrospher 100 RP-18 column (250 × 4 mm, φ = 5 μm; Merck Millipore) at 50°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysate was centrifuged at 5,000 g for 5 minutes at 4 °C and the supernatant was added to ANTI-FLAG M2 Affinity Gel (Sigma-Aldrich), equilibrated with the wash buffer (25 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... The signal was determined by nitroblue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (toluidine salt) (BCIP) (Sigma, 72091).Semithin sections for toluidine blue myelin staining and ultrathin section for TEM imaging were prepared according to our previous protocols (Zhang et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... at 60°C and developed using nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) (Sigma Aldrich) for 60 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... and 10x DMEM (First Link Ltd., UK) were mixed at a 4:1 volume ratio and neutralized with 5 M NaOH (Sigma, Canada). Fast Green (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... and an ATP-regeneration system (4 mM ATP, 50 μg/mL creatine kinase [Sigma-Aldrich], 5 mM creatine phosphate [Sigma-Aldrich]). The endpoint fluorescence for complete FITC-casein degradation was determined by addition of porcine elastase (100 μg/mL ...
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bioRxiv - Neuroscience 2019Quote: ... and spinal cords of both sexes (n = 3 each) were fixed in 4% PFA and embedded in 5% agarose (Type IX-A; Sigma-Aldrich) supplemented with 20% sucrose ...
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bioRxiv - Developmental Biology 2020Quote: ... Fertilized oocytes were collected from 3-4 week-old superovulated C57Bl6 females prepared by injecting 5 IU each of pregnant mare serum gonadotrophin (PMSG) (Sigma Aldrich) and human chorionic gonadotropin (hCG ...
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bioRxiv - Synthetic Biology 2019Quote: ... Sigma-Aldrich and 50 µg mL−1 of 5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside (X-Gal) (no. B4252; Sigma-Aldrich).
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bioRxiv - Cell Biology 2019Quote: ... The nuclei were stained with DAPI (1mg/ml for 5 minutes) and the coverslips were mounted on glass slides in Mowiol® 4-88 (Sigma) mounting medium containing 1mg/ml p-phenylenediamine (Sigma).
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bioRxiv - Neuroscience 2021Quote: ... The flies were then transferred into vials containing Drosophila instant food (Formula 4-24 Instant Drosophila Medium, Carolina Biological Supply Company, USA) containing 5% H2O2 (Sigma, H1009), or vials containing Drosophila instant food only as a control ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were incubated with rabbit UPF1 antibody (CST #9435) antibody used at 1:1000 dilution in 5% BSA 1X TBST overnight at 4°C or mouse Tubulin (Sigma T9206) used at 1:2000 dilution in 5% milk 1X TBST for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM ATP (Sigma Aldrich)] and then incubated with gently sonicated anti-FLAG beads to allow binding for 1 hr at 4°C on a rotator in the presence of oxygen scavenging reagents (5 mg/mL b-D-glucose (Sigma Aldrich), 0.25 mg/mL glucose oxidase (Sigma Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... a 1:10,000 anti-rabbit dilution of IgG alkaline phosphatase was used before detection with NBP-BCIP (nitroblue tetrazolium chloride–5-bromo-4-chloro-3′-indolylphosphate; Sigma-Aldrich) as the substrate.
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bioRxiv - Plant Biology 2021Quote: ... sucrose (#S/8600/60, Fisher) and 100 μM 3’,5’-dimethoxy-4’-hydroxyacetophenone (acetosyringone) (#115540050, Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (#D8418, SIGMA)) ...
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bioRxiv - Plant Biology 2021Quote: ... immunodetection of the DIG-labelled probes was carried out by using an anti-DIG antibody coupled with alkaline phosphatase and a ready-to-use 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro-blue tetrazolium (NBT) solution (Sigma-Aldrich) as the chromogenic substrate for the enzyme ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed twice with PBS (1x PBS, 5 min) and then fixed in 4% PFA (Paraformaldehyde, Sigma Aldrich, Dorset, UK) for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... female mice between 4 and 16 weeks old were superovulated by intraperitoneal injection of 5 IU pregnant mare serum gonadotropin (PMSG, Sigma, G4877) and 48 hours later ...
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bioRxiv - Bioengineering 2022Quote: ... Bone-marrow derived hMSCs (hBMSCs) were frozen at passage 3 or 4 with 5*106 cells/ml in freezing medium containing FBS (BCBV7611, Sigma-Aldrich) with 10% DMSO and stored in liquid nitrogen until further use ...
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bioRxiv - Developmental Biology 2020Quote: ... The staining reactions lasted between 2h and 8h at room temperature using nitro-blue tetrazolium (NBT) and 5-bromo-4-chloro-3′-indolyphosphate (BCIP) staining reactions (Sigma Aldrich). Embryos were then rinsed in PBS-T (0.05% Tween-20) ...
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bioRxiv - Microbiology 2020Quote: ... The fractions containing the complexes were incubated for 1 hr at 4°C with 10 mM N-hydroxysulfosuccinimide and 5 mM EDC (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were incubated overnight at 4°C in TBS Tween (TBST) plus 5% powdered milk and probed with either anti-DNP (Millipore; S7150) or anti-β-actin (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... and placed in Petri dishes filled with 0.5% agarose containing 35 mM β-mercaptoethanol (v/v) and 1.5 mg/mL of 5-bromo-4-chloro-3-indolyl-β-D-galactoside (Sigma). For X-gal staining ...
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bioRxiv - Cell Biology 2019Quote: ... for 1 h at RT and developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-brom-4-chlor-3-indoxylphosphate (BCIP; Sigma-Aldrich) for 5-30 min at RT ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were applied overnight at 4 °C.” Sections were washed in 0.1M PBS and blocked with 5% Chemiblocker (Millipore, Darmstadt,Germany) comprising 0.5% Triton X-100 and 0.05% NaN3 in PBS at pH=7.4 ...
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bioRxiv - Genetics 2020Quote: ... scales were mounted on a microscope slide containing 10 % (w/v) Mowiol® 4-88 (Sigma-Aldrich cat# 9002-89-5) in 25% (v/v ...