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Citations for Millipore Sigma :
2551 - 2600 of 10000+ citations for 6 Hydroxy 2 4 5 triaminopyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: SUM159PT spheres were dissociated by TrypLE™ and plated in mammosphere media (DMEM-F12, 10 ml/500 ml B27 [Invitrogen], 5 µg/ml bovine insulin [Sigma], 4 µg/ml heparin [Sigma] ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at 4°C overnight with the following primary antibodies in 5% BSA in TBST: puromycin (Sigma, 1:25,000) and vinculin (Cell Signaling Technology ...
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bioRxiv - Genomics 2021Quote: ... Samples were then centrifugated at 500g for 5 minutes at 4°C and then were resuspended in 1mL Nuclei PURE Storage Buffer (Nuclei PURE storage buffer, Sigma). Sample washing was performed until the supernatant cleared ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... coli DH5α cells were transformed with the resulting pho-lac fusion plasmids and streaked on dual indicator plates containing LB agar with 5-bromo-4-chloro-3-indolyl phosphate disodium salt (X-Phos) (Sigma) at a final concentration of 80μg/ml and 6-chloro-3-indolyl-β-D-galactoside (Red-Gal ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with a cytometry buffer solution (PBS with 5 % FBS) then fixed with a 4 % formaldehyde (Sigma-Aldrich) for 15 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: Fat bodies were dissected from larvae at 5 or 12 days AEL fixed in 4% PFA and counterstained with Nile Red (Sigma), Phalloidin-488 (Invitrogen ...
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MEK inhibitor resistance in lung cancer cells associated with addiction to sustained ERK suppressionbioRxiv - Cancer Biology 2022Quote: ... Membranes were incubated in primary antibodies (1:1000) overnight at 4° in 5% bovine serum albumin (BSA) (Sigma, A9647-100G), washed with TBST ...
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bioRxiv - Developmental Biology 2022Quote: ... adult DN mutant and Ctrl sibling zebrafish were bathed for 24 h in the presence of 5 μM 4-HT (H7904; Sigma) made from a 10 mM stock solution dissolved in 100% ethanol at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Immunoreactive bands were detected by the addition of BCIP (5-bromo-4-chloro-3-indolylphosphate)-nitroblue tetrazolium solution (Sigma-Aldrich). The reaction was stopped after 2 min by washing the blots with large volumes of deionized water.
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bioRxiv - Bioengineering 2021Quote: ... slides were either treated with SIGMA FAST™ BCIP/NBT (5-Bromo-4-chloro-3-indolulphosphate/Nitro blue tetrazolium, pH 9.5, Sigma) and counterstained with nuclear fast red (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... Sonicated lysates were pelleted for 5 min at 14000 rpm in a microcentrifuge and the entire supernatant was transferred to a new microfuge tube and incubated overnight at 4°C with 5 µg of anti-Myc antibody (9E10) and 20 µl of protein G magnetic beads (Millipore). Following IP ...
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bioRxiv - Molecular Biology 2019Quote: ... Color was developed with freshly prepared 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium (BCIP/NBT; Sigma-Aldrich) and the reaction was stopped by addition of 2 mM ethylenediaminetetraacetic acid (EDTA).
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bioRxiv - Plant Biology 2021Quote: ... Then the proteins were concentrated to 5∼10 mg/ml using the 30 kDa cut-off Amicon Ultra-4 centrifugal filter unit (Millipore), and stored at −80 °C for crystallization ...
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bioRxiv - Cancer Biology 2020Quote: ... Excess TMT reagent was quenched by the addition of 4 μl of an aqueous solution of 5% hydroxylamine (Sigma, 438227). Mixed peptides were subjected to a reverse phase clean-up step (OASIS HLB 96-well μElution Plate ...
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bioRxiv - Immunology 2020Quote: ... CD11c-DTR BM or CD11c-DTR Rag2−/− BM chimeric mice were injected (i.p.) with 4-5 ng/g body weight diphtheria toxin (DT, Sigma-Aldrich) every other day for the indicated time ...
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bioRxiv - Neuroscience 2020Quote: ... and were either incubated with primary antibodies in blocking solution (5% donkey serum and 0.5% Triton in PBS) or were embedded in 4% agar (low-gelling agarose, Sigma Aldrich) and sectioned at 120 μm in the vibratome ...
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bioRxiv - Immunology 2020Quote: 8 to 12-weeks old female C57BL/6j were inoculated subcutaneously with 5×105 B16-F10-OVA and conditioned 4 days later with peritoneal injection of 300 mg/kg cyclophosphamide (Sigma). On day 8 ...
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bioRxiv - Cell Biology 2020Quote: ... Excess TMT reagent was quenched by the addition of 4 μl of an aqueous solution of 5% hydroxylamine (Sigma, 438227). Peptides were mixed to achieve a 1:1 ratio across all TMT-channels ...
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bioRxiv - Developmental Biology 2020Quote: ... After blocking overnight with pH 8.0 Tris-buffered saline with 0.1% Tween20 containing either 4% non-fat dry milk (Carnation) or 5% bovine serum albumin (Sigma-Aldrich), membranes were incubated with the appropriate primary antibody diluted in blocking buffer (4% milk or 5% BSA in 1X TBST ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed in 4% PFA and stained with a phospho-Histone H3 primary antibody (1:1000; Millipore #5-806) overnightat 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... received bilateral infusions of either vehicle (aCSF; pH 7.4; males n = 5; females n = 4) or the GABAA receptor agonist muscimol (Sigma Aldrich, M1523 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were incubated O/N at 4°C followed by two washing steps of 5 minutes with Wash Buffer A (Sigma) at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The fluid was centrifuged at 500xg for 5 min at 4°C and resuspended in RBC lysis buffer (Sigma, R7757) for 5 min at room temperature ...
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Mitochondrial dysfunction rapidly modulates the abundance and thermal stability of cellular proteinsbioRxiv - Biochemistry 2022Quote: ... Excess TMT reagent was quenched by the addition of 4 μl of an aqueous solution of 5% hydroxylamine (Sigma, 438227). Peptides were mixed ...
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bioRxiv - Biochemistry 2022Quote: ... Excess TMT reagent was quenched by the addition of 4 µl of an aqueous solution of 5% hydroxylamine (Sigma, 438227). Peptides were mixed to achieve a 1:1 ratio across all TMT-channels ...
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bioRxiv - Immunology 2022Quote: ... 8-15-weeks-old female C57BL/6J/Ly5.2 were inoculated subcutaneously with 0.5 x 106 B16-F10-OVA and conditioned 4 days later with peritoneal injection of 300 mg/kg cyclophosphamide (Sigma). On day 7 ...
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bioRxiv - Microbiology 2022Quote: ... Cell pellets were resuspended in 0.4 ml of sonication buffer (20 mM Tris pH 8.0, 5 mM EDTA, 1x EDTA-free protease inhibitors [Sigma Aldrich]) and subjected to sonication at 4.5-micron amplitude for 7 cycles of 15 seconds on/15 seconds off ...
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bioRxiv - Plant Biology 2022Quote: ... The reproductive organs were exposed by removing the sepals and placed into glass capillaries (size 4, Zeiss) containing ½ MS medium (5% sucrose, pH 5.8) with 1% low melting point agarose (Sigma Aldrich). The inflorescence embedded in solidified MS was pushed out of the capillary and then placed into the capillary holder for the Z.1 ZEISS light-sheet microscope ...
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bioRxiv - Plant Biology 2022Quote: ... pH 5.5 and 100 μM 3′,5′-dimethoxy-4′-hydroxyacetophenone (acetosyringone) (115540050; Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (D8418; Sigma). The culture was incubated with shaking (120 rpm ...
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bioRxiv - Immunology 2022Quote: ... the cells were firstly cultured for 4 h in a 37°C/5% CO2 incubator with 50 ng/ml PMA (Sigma) and 1 mM ionomycin (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei and other debris were pelleted at 2,000 g for 5 min at 4°C and the supernatant was filtered through three layers of 100 μm pore nylon mesh (Millipore), and a 5 μm pore PVDF syringe filter (Millipore) ...
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bioRxiv - Biochemistry 2022Quote: ... and phosphatases (1.15 mM sodium molybdate, 4 mM sodium tartrate dihydrate, 1 mM freshly prepared sodium orthovanadate, 5 mM glycerophosphate, all Sigma), and 100 mM N-Ethylmaleimide (NEM ...
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bioRxiv - Molecular Biology 2022Quote: ... for one hour and incubated overnight at 4°C in 5% milk/PBS-T with the following antibodies: anti-LIMCH1 (1:400, catalog number HPA063840, Sigma), anti-MBNL1 (1:1,000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were reconstituted in 100 µL and 2 µL of the sample was injected from a 4 °C autosampler into a ZIC-pHILIC 150 × 2.1 mm 5 µm particle size column (EMD Millipore) with a ZIC-pHILIC 20 x 2.1 guard column in a Vanquish Duo UHPLC System (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... Embryos were then incubated overnight at 37°C under 5% CO2 in humidified air in 4-well culture dishes containing KSOM media (Millipore). Experiments were approved by the SIMR IACUC and were performed following the committees’ guiding principles.
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bioRxiv - Neuroscience 2024Quote: ... and blocked with 5% milk in TBS-Tween 20 for 2h and incubated with primary antibodies diluted in the 5% BSA in TBS-Tween 20 overnight at 4°C (AMPD2, Sigma HPA045760 ...
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bioRxiv - Developmental Biology 2024Quote: ... Tg(-3.5ubb:loxP-GFPloxP-mCherry)cz1701Tg embryos were incubated in embryo media containing 5 μM (Z)−4-Hydroxytamoxifen (Sigma-Aldrich H7904) starting at 4-4.5 hours post-fertilization (hpf ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were incubated in NBT (nitro-tetrazolium blue)-BCIP (5 bromo-4-chloro-3’-indolyphosphate p-toluidine) (Sigma-Aldrich), 0.033% in APTMg in dark condition ...
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bioRxiv - Cell Biology 2023Quote: ... The lysate was clarified by centrifugation at 20,000 × g for 5 min at 4°C and filtered on an Ultrafree-MC GV Centrifugal filter (Millipore, UFC30GV0S). Superose 6 Increase HR 10/300 column (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were washed in 1xPBS for 5 minutes and coverslipped with 75µl of mounting media (50% glycerol – Sigma, G5516 and 4% propyl gallate – Sigma, 2370).
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bioRxiv - Immunology 2022Quote: ... RT complete IntestiCult containing 25μg/ml was added, and enteroids were incubated (37°C, 5% CO2) until fixation with 4% paraformaldehyde (PFA; Sigma Aldrich) at 4h p.i ...
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bioRxiv - Microbiology 2023Quote: ... and remaining lysate was end-over-end incubated with 5 µg of primary Ab overnight at 4°C [Rabbit-anti-HA (Sigma), Rabbit-anti-Flag (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... 537-98-4] were added to the media from 25 mg/mL stock (in ethanol [Sigma Aldrich, 64-17-5]), and the final ethanol concentration was adjusted to 1.86% v/v for all conditions ...
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bioRxiv - Biochemistry 2023Quote: ... Excess TMT reagent was quenched by the addition of 4 µl of an aqueous solution of 5% hydroxylamine (Sigma, 438227). Peptides were mixed to achieve a 1:1 ratio across all TMT-channels ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed 5 times in PB 0.1 M and mounted with a mounting solution (Mowiol 4-88, Sigma-Aldrich).
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bioRxiv - Immunology 2023Quote: ... CD11c-DTR/GFP BM chimeric mice were injected (i.p.) with 4–5 ng/g body weight diphtheria toxin (DT, Sigma-Aldrich) every other day for 14 days.
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bioRxiv - Developmental Biology 2023Quote: ... 4- to 5-week-old donor rats were intraperitoneally injected with 30 units of equine chorionic gonadotropin (G4877, Sigma-Aldrich), followed by an intraperitoneal injection of 30 units of human chorionic gonadotropin (C1063 ...
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bioRxiv - Cell Biology 2022Quote: ... The conditioned media was spun down for 5 minutes at 800g and 4°C and then filtered with a syringe and 0.22 μm filter (Millipore, SLGV033RS) and stored at 4°C for no more than three days before being applied to cells ...
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bioRxiv - Genetics 2022Quote: ... K562 cells were transduced at a cell concentration of 5×105 cells/ml in the culture medium supplemented with 4 μg/ml polybrene (Sigma). After 24 h ...
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bioRxiv - Molecular Biology 2023Quote: The sorted cells were concentrated by centrifugation at 300 x g for 5 minutes at 4 °C and resuspended in DPBS (Sigma) with 0.04% w/v BSA (Sigma) ...