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Citations for Millipore Sigma :
2851 - 2900 of 10000+ citations for 6 Hydroxy 2 4 5 triaminopyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Cdc5-3V5 and Rim4-3V5 (control non-kinase tagged protein) were immunoprecipitated at 4°C 2 hours using 20 µL of anti-V5-agarose slurry (Sigma). Purifications were washed 4 times with NP40 buffer.
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bioRxiv - Plant Biology 2021Quote: ... 1 g of the infiltrated leaf tissue was ground in 4 mL of extraction buffer (1/2 tablet of Sigma Complete™ mini protease inhibitor (per 15 mL) ...
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bioRxiv - Microbiology 2021Quote: Fifty microliters containing 3.5 x 106 PFU/ml SARS-CoV-2 viral stock was placed on one well of a 4-well chambered glass slide (Nunc, Sigma) (Fig 2) ...
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bioRxiv - Neuroscience 2020Quote: ... 66 These transgenic mice express the GEVI in cortical layer 2/3 pyramidal neurons under the intersectional control of TetO and destabilised Cre-recombinase.70–72 The destabilised Cre-recombinase was stochastically re-stabilised to induce sparse expression of the voltage indicator via two IP injections of a total of 2 × 10−4 mg kg−1 Trimethoprim (TMP, Sigma) over 2 consecutive days as described in Song et al ...
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bioRxiv - Biophysics 2021Quote: ... The virus-containing supernatant was concentrated by ultracentrifugation in a SW-28 rotor (25,000 rpm, 2 h, 4°C) using OptiPrep density gradient medium (Sigma-Aldrich). Part of the supernatant containing pelleted viruses was collected ...
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bioRxiv - Neuroscience 2022Quote: Headless larvae (7 dpf) were fixed for three days at 4 °C in EM fixation buffer [2% EM-grade glutaraldehyde (Sigma), 2% PFA (Sigma) ...
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bioRxiv - Microbiology 2022Quote: Sialidase activity was fluorometrically monitored using the substrate 2’-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4MU-Neu5Ac; Sigma-Aldrich). Reactions were performed in a final volume of 50 μL ...
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bioRxiv - Biochemistry 2022Quote: ... membranes were equilibrated at 4 °C and incubated for 30 min in the presence of 2 mg/ml iodoacetamide (Sigma). Membranes were then solubilized in 10 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... mice were fasted for 4 hours followed by gavaging of 2 g/kg (10 ml/kg) of D-glucose (Sigma) dissolved in distilled water ...
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bioRxiv - Immunology 2020Quote: ... A total of 5 × 105 splenocytes was ex vivo restimulated with the full-length S peptide mix (0.1 µg/mL final concentration per peptide) or controls (gp70-AH1 [SPSYVYHQF]38, 4 µg/mL; concanavalin A [ConA], 2 µg/mL [Sigma]). Streptavidin-alkaline phosphatase (ALP ...
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bioRxiv - Immunology 2020Quote: ... A total of 5 × 105 splenocytes was ex vivo restimulated with the full-length S peptide mix (0.1 μg/mL final concentration per peptide) or controls (gp70-AH1 [SPSYVYHQF]33, 4 μg/mL; Concanavalin A [ConA], 2 μg/mL [Sigma]). Streptavidin-alkaline phosphatase (ALP ...
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bioRxiv - Microbiology 2021Quote: ... 1X Pen-Strep and 1 μg/mL L-[(toluene-4-sulphonamido)-2-phenyl] ethyl chloromethyl ketone (TPCK)-trypsin (Sigma-Aldrich). The CPE was observed by visual microscopy at 3 and 5 days post-infection (d.p.i. ...
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bioRxiv - Microbiology 2020Quote: ... supernatant was collected in clean microcentrifuge tube and incubated 2 h at 4°C with a-flag mouse antibody (Sigma), following 1 h incubation at 4°C with PureProteomeProtein A/G Magnetic Beads Mix (Millipore) ...
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bioRxiv - Physiology 2021Quote: ... was incubated for 2-3 h at 4 °C with 30 µl of FLAG-conjugated beads (anti-DYDDDDDK beads, Sigma) or empty beads (ThermoScientific) ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysate was immunoprecipitated for 2 hours at 4°C with the following antibodies: rabbit anti-myc (2.5 μg, Millipore Sigma), mouse anti-HA (2.5 μg ...
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bioRxiv - Neuroscience 2020Quote: ... 0.3% TritonX/PBS) for 2 hours and stained with primary antibodies overnight at 4°C (guinea pig anti-vGlut2, 1:1000, Millipore AB2251-I ...
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bioRxiv - Developmental Biology 2019Quote: 2-[4-(Dimethylamino)styryl]-1-ethylpyridinium iodide (DASPEI) labeling in live fish was performed by diluting 1mM DASPEI (Sigma-Aldrich) in system water ...
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bioRxiv - Genomics 2019Quote: Human liver specimens obtained from resections were perfused for 15 minutes with calcium-free 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid buffer containing 0.5 mM ethylene glycol tetraacetic acid (Fluka) followed by perfusion with 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid containing 0.5 mg/mL collagenase (Sigma-Aldrich) and 0.075% CaCl2 at 37°C for 15 min as previously described53 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... membranes were blocked for 1 h at RT in 5% skimmed milk powder and 0.2% Tween in PBS before incubating overnight at 4◦C with the primary antibodies mouse anti-TH (1:1000, Millipore) and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... The re-suspensions were spread on 7H11 OADC plates supplemented with the relevant drug concentration (2, 4, or 8 µg/mL of ofloxacin, or 100 µg/mL streptomycin; Sigma). Resistant colonies were observed and enumerated after 21 to 35 days of incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... Concentrations of 29 cytokines/chemokines were measured in 2-4 biological replicates using MILLIPLEX Map Human Cytokine/Chemokine Magnetic Bead Panel (HCYTMAG-60K-PX29, #Millipore) according to manufacturer’s protocol.
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bioRxiv - Plant Biology 2020Quote: ... stratified for 2 d at 4°C in the dark and sown on Gamborg’s B5 Basal Salt medium (Sigma G5768) supplemented with 1% (w/v ...
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bioRxiv - Microbiology 2019Quote: ... 4°C) and resuspended in binding buffer (50 mM Tris, 1 mM MgCl2, 2 mM imidazole, 1 mM PMSF (Sigma), 300 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... The flow-through from the His-tag cleavage containing the tag free protein was concentrated to 2 mL using Amicon Ultra-4 30k MWCO (Millipore). The concentrated protein was applied to a HiLoad 16/600 S75pg column (GE Life Sciences) ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated with 10μM 1,2-dioleoyl-sn-glycero-3-phospho-L-serine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (18:1 NBD-PS) (1:300, Sigma-Aldrich) for 15 minutes at 25°C ...
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bioRxiv - Microbiology 2019Quote: ... and media with 4 mM Ca2+ was made by adding 2 mM CaCl2 to the standard high-glucose DMEM (Sigma). We maintained TV-infected cells under these conditions until the positive control (normal media ...
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bioRxiv - Developmental Biology 2019Quote: 6 dpf Cntnap2a-positive fish were treated for 1 hour with 0.05% DASPEI (2-4-dimethylamino-N-ethylpyridinium iodide; Sigma Aldrich), which labels both superficial and canal neuromasts [46] ...
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 2 h with protein G Sepharose beads (Millipore). Normal mouse or rabbit IgG (Santa Cruz ...
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bioRxiv - Microbiology 2021Quote: ... at 25,000 x g for 2 h at 4°C on a 20% sucrose cushion (Sigma-Aldrich, St. Louis, MO). Media and sucrose were discarded ...
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bioRxiv - Biochemistry 2021Quote: ... The second half of the clarified lysate was incubated for 2 h at 4°C with 20 μl of Anti-FLAG M2 magnetic beads (Sigma) equilibrated in FLAG wash buffer containing 20 mM HEPES pH 8.0 ...
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bioRxiv - Pathology 2021Quote: ... at 25,000 x g for 2 hours at 4 °C on a 20% sucrose cushion (Sigma-Aldrich, St. Louis, MO). Media and sucrose were decanted ...
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bioRxiv - Neuroscience 2021Quote: ... Following electrophoresis, proteins were transferred (100 V, 2 hr, 4°C) to polyvinylidene difluoride membranes (Immobilon-FL, Millipore, Billerica, MA). To confirm protein transfer ...
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bioRxiv - Developmental Biology 2022Quote: ... Each sample was incubated with primary antibodies for 1–2 h at room temperature or overnight at 4°C and then with fluorescent-conjugated secondary antibodies and DAPI (Sigma) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... femurs and tibias of C57BL/6J mice were extracted and crushed on a mortar in PBS supplemented with 4%FBS and 2 mM EDTA and filtered through 0.45μm strainers (Merck Millipore, Cat#SLHV033RB). For B cells ...
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bioRxiv - Biochemistry 2022Quote: ... and cell pellets were collected and resuspended in the resuspension buffer containing 20 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (Sigma) pH 7.4 and 200 mM NaCl (Sigma)] ...
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bioRxiv - Neuroscience 2022Quote: ... 500 μg of total protein from PC12 cell extracts were incubated over night at 4°C with 2 μg of the phospho- tyrosine 4G10 (Sigma-Alrich/Upstate Cat# 05-321 ...
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bioRxiv - Neuroscience 2022Quote: ... Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM, Sigma-Aldrich, P8340) and was added to each frozen brain section on ice ...
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bioRxiv - Molecular Biology 2022Quote: 400 μl of cell lysate was incubated overnight at 4 °C with 2 μg of anti-Myc or anti-Flag antibody (Sigma). Protein A-Sepharose (GE ...
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bioRxiv - Cancer Biology 2024Quote: ... 80 μM aprotinin, 4 mM bestatin, 1.4 mM E-64, 2 mM leupeptin and 1.5 mM pepstatin A; Sigma-Aldrich, P8340) and 50 nM calyculin A (LC Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM, Sigma-Aldrich, P8340) and was added to each frozen brain section on ice ...
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bioRxiv - Neuroscience 2024Quote: ... Media was incubated at 4 °C for 2 hours in 20% PEG solution (40% w/v stock: 2.5 M NaCl, 40% PEG (Sigma P2139) followed by a 20 min 3,000 x g spin at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... tricaine-anesthetized 3 or 4 days-post fertilization (dpf) larvae were mounted in 2% low-melting point agarose (Sigma-Aldrich) on a 35 mm glass bottom dish (#1.5H Glass ...
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Physical activity increases neuronal activity in the circadian clock of diurnal Arvicanthis ansorgeibioRxiv - Neuroscience 2024Quote: ... The brain was then removed and fixed for 2 days in 4% paraformaldehyde containing C6FeK4N6·3H20 (Sigma-Aldrich, Lyon, France). Coronal slices (40 µm thick ...
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bioRxiv - Cell Biology 2023Quote: ... except the protein sample was concentrated to 4 mg/ml and mixed with 0.005% 3-([3-Cholamidopropyl]dimethylammonio)-2-shydroxy-1-propanesulfonate (CHAPSO; Sigma Aldrich) immediately before vitrification ...
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bioRxiv - Developmental Biology 2023Quote: We scanned formalin-fixed whole kidneys stained for 2 hours in 4% OsO4 solution (Sigma-Aldrich, St. Louis, MO, USA) using a previously developed method 31 ...
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PQS and Pyochelin in Pseudomonas aeruginosa Share Inner Membrane Transporters to Mediate Iron UptakebioRxiv - Microbiology 2023Quote: ... A total of 100 µL of 4 mg/mL 2- nitrophenyl β-D-galactopyranoside (ONPG) (Sigma, St. Louis, MO, USA) was added to the cells ...
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bioRxiv - Molecular Biology 2023Quote: The feeder-dependent murine ESC line v6.5 and feeder free Bruce-4 cells were cultured in 2% gelatin-coated (SIGMA, G1393) ventilated-cap flasks ...
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bioRxiv - Cell Biology 2023Quote: ... (phenylmethylsulfonyl fluoride, PMSF 1mM; 1-chloro-3-tosylamido-4-phenyl-2-butanone, TPCK, 10 μg/ml; aprotinin, 10 μg/ml Sigma) incubated for 30min on ice ...
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bioRxiv - Cell Biology 2023Quote: ... Subcultivation was performed every 2-4 days at a confluence of maximum 80 % using trypsin (Sigma-Aldrich, cat.no. 59427C-100ML). HEK293T-LentiX (Takara Bio ...
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bioRxiv - Bioengineering 2023Quote: ... sulfo-succinimidyl-4-(N-maleimidomethyl)cyclohexane-l-carboxylate (sulfo-SMCC, Sigma, at a concentration of 2 mg/mL in PBS) were added to the emulsion and kept to react for 1 h at room temperature ...