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Citations for Millipore Sigma :
2901 - 2950 of 10000+ citations for 6 Hydroxy 2 4 5 triaminopyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The supernatant was concentrated using ultracentrifugation in an SW-28 rotor (25,000 rpm, 2 h at 4°C) on a 60% OptiPrep density cushion (Sigma-Aldrich). The pelleted viruses were resuspended in 10 mL TNE buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... cells were centrifuged with 293T retroviral supernatant at 1000×g for 2 hr at 37°C in the presence of 4 µg/mL polybrene (Sigma). Following spin infection ...
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bioRxiv - Biochemistry 2024Quote: ... Lysate was clarified by centrifugation and incubated for 2 hours at 4 °C with anti-FLAG M2 agarose beads (Sigma) with gentle rotation ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then washed 3 times for 20 - 40 minutes each wash in PBS before incubation with secondary antibody(s) in blocking solution for 2 - 4 hours at room temperature (Alexa 647-conjugated donkey anti-chicken, AP194SA6, Millipore – to label GFP ...
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bioRxiv - Bioengineering 2024Quote: ... and polyIC incubation was evaluated using the MTT (3-(4, 5dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). The cells were seeded into 96 well-format cell culture plates at a seeding density of 8ξ103 cells per well ...
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bioRxiv - Immunology 2024Quote: ... Next, 50 μl of 2× STOP buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA (cat. E3889, Sigma-Aldrich) and 100 μg/ml RNase A (cat ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... flies were homogenized in 50 μl PBS per fly (between 2-4 flies per sample) containing 0.1% Tween-20 (Sigma #1379) using a TissueLyser LT (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were treated with 2% (74.4 mOsmol/kg) or 4% PEG-300 (148.8 mOsmol/kg; v/v; Millipore Sigma 8074845000) in culture medium for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: MM-NLP particles isolated from both sucrose gradient sedimentation (native) and GraFix (crosslinked) were concentrated to 2 µM using Amicon Ultra-4 centrifugal filters (Ultracel 30K, Millipore). C-Flat 1.2/1.3 (Cu ...
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bioRxiv - Cell Biology 2019Quote: ... the slides with attached intact axonemes were treated overnight at 4°C with blocking buffer containing the diluted primary antibody (mouse anti-acetylated tubulin, Sigma-Aldrich, clone 6-11B-1, 1:1000). On the next day ...
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bioRxiv - Cell Biology 2021Quote: ... 150 μM 4-(4-Morpholinobutylthio)phenol (MoTP) (Sigma) was added to embryos kept at 28.5°C from 24hpf onwards ...
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bioRxiv - Biochemistry 2020Quote: ... and 4×10^-4 1-Thioglycerol (Sigma Aldrich), and incubated for 72 hours ...
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bioRxiv - Neuroscience 2020Quote: ... 4-AP (4-Aminopyridine, 500 μM, Sigma-Aldrich), FK-506 (1 μM ...
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bioRxiv - Bioengineering 2019Quote: ... (ii) 4 hours of 4% sodium deoxycholate (Sigma) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... 4-Hydroxytamoxifen (4-OHT) was purchased from Sigma and dissolved in ethanol ...
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bioRxiv - Immunology 2021Quote: 4-hydroxytamoxifen (Tx) (4-OHT, #T5648, Sigma-Aldrich) was dissolved in sunflower oil to a concentration of 10 mg/mL for i.p ...
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bioRxiv - Neuroscience 2022Quote: (Z)-4-Hydroxytamoxifen (4-OHT; Sigma-Aldrich #H7904) was first prepared as 25 mg/mL stock solutions in ethanol and frozen at −20C ...
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bioRxiv - Neuroscience 2024Quote: ... and 4-aminopyridine (4-AP, 100 μM, Sigma), mecamylamine (10 μM ...
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bioRxiv - Biochemistry 2023Quote: ... 4 mM MgCl2 and 4% DMSO (Sigma, 276855). The mixture was incubated for 30 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... Chemicals used: 4-Aminopyridine (4-AP, Sigma #A78403), tetrodotoxin (TTX ...
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bioRxiv - Developmental Biology 2023Quote: ... 4-hydroxytamoxifen (4-OHT, Cat#: H7904, Sigma-Aldrich) was dissolved at 10mg/ml in peanut oil and 1mg was administered by intraperitoneal injection from E10.5 for 4 consecutive days ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-hydroxytamoxifen (4-OHT; 1 μM; #H7904 Sigma) or vehicle was added to the cell culture medium to activate Cre-LoxP recombination ...
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bioRxiv - Microbiology 2023Quote: ... 3-ethoxy-4-hydroxybenzaldehyde (121-32-4, Sigma), N-(acid-PEG10)-N-bis(PEG10-azide ...
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bioRxiv - Cell Biology 2024Quote: ... 4-hydroxytamoxifen (4-OH TAM, Sigma-Aldrich, H7904), L-selenocystine (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... 2.2 mW.mm-2 for 5-10 ms) in control and in the presence of 1 µM strychnine (Sigma-Aldrich), 20 µM DNQX and 10 µM gabazine (BioTechne ...
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bioRxiv - Developmental Biology 2021Quote: ... fixed with 0.2% gluteraldehyde/4% PFA at room temperature and incubated overnight in hybridization buffer (5% Dextran sulphate, 2% blocking powder from Roche, 5X SSC, 0.1% TritonX, 0.1% CHAPS from Sigma Aldrich, 50% formamide ...
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bioRxiv - Neuroscience 2019Quote: ... IMR-32 neuroblastoma were differentiated for 15-16 days using the above media supplemented with 5-Bromo-2’-deoxyuridine (BrdU; 2.5 µM; Sigma) and N6 ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were washed with PBS and incubated for 2 h in blocking solution (0.1 M PBS, 0.25% Triton, 5% normal goat serum, Sigma Aldrich). Tissue was treated with primary antibody ...
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bioRxiv - Plant Biology 2019Quote: Detection of Ca2+ in stomata was performed using the Fura-2 AM dye (Sigma Aldrich, CAS 108964-32-5) (Jiang et al. ...
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bioRxiv - Neuroscience 2019Quote: ... R-(+)-[(2-n-butyl-6,7-dichloro-2-cyclopentyl-2,3-dihydro-1-oxo-1H-inden-5-yl)oxy] acetic acid (DIOA; Sigma-Aldrich) and VU0240551 (VU ...
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bioRxiv - Molecular Biology 2019Quote: ... and 10 glucose (pH 7.35) up to 5 min before being transferred to 2% agarose low gelling temperature (Sigma) at 32°C ...
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bioRxiv - Biochemistry 2020Quote: ... After formation of the CAK-MAT1Δ219-THZ1 complex by incubation of 0.2 mg/mL CAK-MAT1Δ219 (approx. 2 μM) with 5 μM THZ1 (EMD Millipore) for 1.5 h at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... were added to 1 mL of a 0.1M solution of 5-norbornene-2-carboxylic acid in 0.1M phosphate buffer (pH 6.0) (all reagents purchased from Sigma-Aldrich). The solution was allowed to react at room temperature with intermittent vortexing ...
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bioRxiv - Cell Biology 2021Quote: ... 12-24hr embryo lysates were collected by homogenizing dechorionated embryos in a Dounce homogenizer with 100 μl of lysis buffer (60mM Tris pH7.5, 80mM NaCl, 6mM EDTA pH8.0, 2% Triton X-100, 5mM 1-Naphthyl potassium phosphate, 2mM PMSF, 1X Sigma Phosphatase Inhibitor II ...
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bioRxiv - Neuroscience 2020Quote: ... mice were pulsed with 100 µg per gram of body weight of 5-Ethynyl-2’-deoxyuridine (EdU) (Sigma-Aldrich) at 24 hours before sacrifice by injection intraperitoneally.
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bioRxiv - Microbiology 2021Quote: ... livers were finely minced with a sterile razor blade and digested overnight at 37°C in 40 mL 1×PBS containing 2% penicillin/streptomycin/amphotericin B (1×PBS −2% PSF) and 5 mL 0.5% clostridial collagenase solution (0.025 g of collagenase (Sigma, C0130) in 5 mL of dH20 for immediate use) ...
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bioRxiv - Cancer Biology 2021Quote: ... migrating/invading/transmigrating cells were incubated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 5 mg/ml, 1/10 volume; Sigma-Aldrich) added to the culture medium at 37°C for 4h ...
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bioRxiv - Cell Biology 2021Quote: ... the mouse anti-alpha-tubulin B-5-1-2 was used at a dilution of 1:500 (Sigma, T5168). Polyclonal rabbit anti-TgCentrin1 (Fig ...
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bioRxiv - Biophysics 2020Quote: Zebrafish larvae at 5 days post-fertilization (dpf) were immobilized in 2% low melting point agarose (LMA) (Sigma-Aldrich) on microscope slides ...
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bioRxiv - Cell Biology 2020Quote: ... the hSCOs were either cultured in DM containing AEDs (valproic acid, 0.5 or 1 or 2 mM, Sigma, P4543; carbamazepine, 5 or 50 or 100 μM, Sigma, C4024 ...
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bioRxiv - Cell Biology 2020Quote: ... all the cell lines stably expressing inducible lenti vector were induced with 2-5 μg/ml of doxycycline (Sigma) for 24–96 h.
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bioRxiv - Neuroscience 2022Quote: ... Hippocampal neurons were cultured at a density of 5 × 104 cells.cm−2 on glass coverslips coated with 0.04% polyethyleneimine (Sigma, PEI) in Dulbecco⍰s Modified Eagle⍰s Medium (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... and NMDA receptor mediated PSCs were blocked with DL-2-amino-5-phosphonopentanoic acid (D-AP5; 50 μM; Sigma). The AMPA-R PSC frequency and amplitude was determined from a 1 min interval after the recording had stabilized (~10 min after wash-in of picrotoxin/D-AP5) ...
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bioRxiv - Microbiology 2022Quote: ... 5 mg/ml of 3-(4,5-Dimethyl-2-thiazolyl-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, San Louis, MI, USA) solution in PBS was added to the cells and incubated for an additional 4 h ...
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bioRxiv - Immunology 2022Quote: ... or 16.67µg Pam2CSK4 plus 2 mM 5-A-RU (as described in [45]) and 50 µM MGO (Sigma-Aldrich) in 50 µl (25µl per nares) ...
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bioRxiv - Plant Biology 2021Quote: Anti α-tubulin antibody (DM1A) and anti α-tubulin antibody (B-5-1-2) were purchased from Sigma-Aldrich and used at ×1,000 dilution for immunostaining (DM1A and B-5-1-2 ...
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bioRxiv - Cell Biology 2021Quote: African green monkey kidney Vero E6 cell and Colorectal Adenocarcinoma human epithelial (Caco-2) cells were maintained at 37 °C in 5% CO2 in Dulbecco’s minimal essential medium (DMEM) (Sigma) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... asynchronously growing subconfluent cells were labeled with 30 μM thymidine analogue 5-chloro-2’-deoxyuridine (CIdU) (#C6891, Sigma-Aldrich) for 20 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used in this study: anti-α-tubulin (clone B-5-1-2 - Sigma, T5168); anti-acetylated histone H3 (K9 ...
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bioRxiv - Pathology 2020Quote: ... the parasites (2 × 106/mL) were incubated with 200 µL L-arginine solution and 5 µM DAF-2DA (Sigma) for 2 h at room temperature ...