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Citations for Millipore Sigma :
2951 - 3000 of 10000+ citations for 6 Hydroxy 2 4 5 triaminopyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated with 5 mg/mL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma Aldrich) in PBS for 15 min at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... at a 1:2000 dilution while tubulin was incubated with anti-Tubulin antibody B-5-1-2 (Sigma-Aldrich) at a 1:1500 dilution ...
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bioRxiv - Developmental Biology 2019Quote: ... rinsed again for 5 minutes in PBX and blocked for 30 minutes to 1h in 2% horse serum (Sigma) in PBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... The material was then rehydrated by adding 1 × PBS for 5 min followed by incubation with 2% driselase (Sigma) for 60 min at 37°C in a moisture chamber to remove the cell wall ...
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bioRxiv - Genetics 2020Quote: ... nematodes were cultured and then assayed for survival on plates with 10 μg/ml 5-fluoro-2-deoxyuridine (Sigma), to avoid vulval rupture [27] and eliminate live progeny ...
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bioRxiv - Biochemistry 2020Quote: ... cells were rinsed three times with assay buffer (8.3 g/L DMEM, 2 mM sodium pyruvate 5 mM dextrose, and 0.75 mM L-glutamine, pH 7.4, all from Sigma-Aldrich). Cells were then incubated at 37°C ...
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bioRxiv - Bioengineering 2019Quote: ... devices were washed twice with DI H2O and then stained for 5 minutes with 2% alizarin red (Sigma-Aldrich) in DI H2O [pH 4.1-4.3] ...
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CCG•CGG interruptions in high penetrance SCA8 families increase RAN translation and protein toxicitybioRxiv - Genetics 2021Quote: ... Membranes were blocked for 2 hours at room temperature in 5% dry milk in 1xPBS containing 0.05% Tween-20 (Sigma) and probed with anti-FLAG antibody (1:2,000) ...
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bioRxiv - Developmental Biology 2021Quote: ... total RNA was isolated from young adult fruit flies (2-5 days) or S2 cells using TRI Reagent (Sigma) according to the manufacturer specifications ...
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bioRxiv - Microbiology 2020Quote: ... FMC Biopolymer (90) in DMEM (containing 2% FCS and antibiotics) and serial dilutions of 5-FU (F6627, Sigma-Aldrich) or Ribavirin (R9644 ...
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bioRxiv - Immunology 2021Quote: ... via subcutaneous injection and boosted 2 weeks later with 5 μg of RBD in 100 μL Incomplete Freund’s Adjuvant (IFA, Sigma). Mice were bled 14 days after the boost ...
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bioRxiv - Immunology 2021Quote: ... the γ-secretase inhibitor (2S)-N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine 1,1-dimethylethyl ester (DAPT; R&D) or dimethyl sulfoxide (DMSO; Sigma) was added to media at the concentrations indicated.
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bioRxiv - Cell Biology 2020Quote: ... They were stained with 2% uranyl acetate for 5 min and embedded in 0.17% uranyl acetate and 0.33% polyvinyl alcohol (P8136, Sigma-Aldrich). After drying up ...
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bioRxiv - Cancer Biology 2021Quote: ... migrating/invading cells were incubated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 5 mg/ml, 1/10 volume; Sigma-Aldrich) added to the culture medium at 37°C for 4h ...
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bioRxiv - Cell Biology 2022Quote: ... (2×103-5×103) siRNA reverse transfected endothelial cells (24h or 48h post transfection) were seeded to fibronectin (Sigma)-coated E-plates (growth ...
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bioRxiv - Microbiology 2022Quote: ... 5 mg/ml of 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, St. Louis, MI, USA) solution was applied to the cells and incubated for an additional 4h ...
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bioRxiv - Immunology 2023Quote: PBMCs were washed twice and adjusted to 2×106 cells/ml in HBSS plus 5% FBS (Sigma-Aldrich F2442). To induce apoptosis ...
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bioRxiv - Developmental Biology 2024Quote: ... exponentially growing cells were pulse-labeled for 20 minutes with 25 µM 5-iodo-2-deoxyuridine (I7125, Sigma-Aldrich), followed by a second 20-minute pulse with 250 µM 5-chloro-2-deoxyuridine (C6891 ...
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bioRxiv - Neuroscience 2022Quote: ... mice were anesthetized and administered fluorescein isothiocyanate (FITC)–dextran (0.1 ml, 2 M MW, 5% w/v, Sigma-Aldrich) via tail veil to label blood vessels ...
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bioRxiv - Immunology 2022Quote: ... cell suspensions were washed and stored in PBS containing 2% FBS (Omnilab) and 5 mM of EDTA (Sigma-Aldrich) for acquisition ...
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bioRxiv - Bioengineering 2024Quote: ... blocked for 2 hr at room temperature in PBSTX (DPBS + 0.1% Triton X-100) containing 5% donkey serum (D9663, Sigma), incubated in primary and then secondary antibodies in blocking buffer for at least 48 hr at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... After blocking of endogenous peroxidases (3% H2O2) and unspecific antibody binding (2% BSA+5%serum) PLAC8 antibody (HPA040465, Sigma) or SARS-CoV-2 Spike Glycoprotein S1 antibody (GTX632604 ...
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bioRxiv - Developmental Biology 2024Quote: ... organoids were washed twice for 5 minutes with 2 mL of SSCT (0.1% Tween20 in 5x SSC buffer, Sigma) at room temperature.
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bioRxiv - Cancer Biology 2024Quote: ... Embryos were transferred to E3 solution containing 5 µg/ml proteases and 0.2 mM1-phenyl-2-thio-urea (Sigma) 24 h post-fertilization to dechorionate the fish embryos and prevent pigmentation ...
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bioRxiv - Molecular Biology 2022Quote: ... Approximately 150 synchronized L4 worms were transferred to plates supplemented with 0.5 μg/ml of 5-fluor-2-deoxyuridine - FUdR (Sigma), an inhibitor of mitosis ...
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bioRxiv - Cell Biology 2023Quote: ... iParkinDA and control mice were treated for 5 consecutive days by intraperitoneal injection of 2 mg of tamoxifen (Sigma T5648 dissolved in ethanol and sunflower oil ...
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bioRxiv - Cell Biology 2023Quote: ... DNA synthesis was quantified by the DNA incorporation of the thymidine analog 5-ethynyl-2′-deoxyuridine (EdU; Sigma Aldrich) (10 μM ...
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bioRxiv - Microbiology 2023Quote: ... 5 mg/ml of 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, St. Louis, MI, USA) solution was added to the cells and incubated for an additional 4 h ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated for 2 hours at 35 °C with 5 μl of 500 mM CaCl2 (Sigma-Aldrich), 250 ng of competence stimulating peptide 1 (CSP-1 ...
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bioRxiv - Bioengineering 2023Quote: ... Di-tert-butyl decarbonate (BoC2O) and 5-norbornene-2-carboxylic acid were purchased from Sigma Aldrich (St. Louis, MO). Irgacure 2959 was purchased from Advanced Biomatrix (Carlsbad ...
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bioRxiv - Neuroscience 2023Quote: ... A solution of 10 mM 5-fluoro-2’-deoxyuridine and uridine (anti-mitotic agent) (Sigma Aldrich, St. Louis, USA) was added to the cultures at days in vitro (DIV ...
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bioRxiv - Biophysics 2023Quote: ... blocked and stained with anti-alpha tubulin primary antibodies (monoclonal mouse clones B-5-1-2 and DM1a, Sigma) overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Fixed embryos were washed 3 times (5 minutes each) in wash buffer DPBS Tween containing 2% BSA (Sigma, A3311), and permeabilised at room temperature for 20 minutes in permeabilisation buffer 0.5% Triton-X in DPBS ...
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bioRxiv - Physiology 2024Quote: ... washed 2 x 5 min in PBS and then incubated with primary antibodies against Laminin-β1γ1 (1:200; #L9393, Sigma) in 3% BSA in PBS ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated at 37°C under 5% CO2 in serum free DMEM containing 2% penicillin/streptomycin (Sigma) and 1 µg/ml TPCK Trypsin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated at 37°C with 5% CO2 in serum free DMEM containing 2% penicillin/streptomycin (Sigma) and 1 µg/ml TPCK Trypsin (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... and blocked for 2 hrs at room temperature in PBSTX (DPBS + 0.1% Triton X-100) containing 5% donkey serum (D9663, Sigma). Following ...
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bioRxiv - Cancer Biology 2023Quote: Culture medium was replaced with 100 µL medium containing 5 mg/mL filtered 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (#M5655, Sigma-Aldrich) and incubated for 2 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... 30 μL of MTT [(3- [4,5-dimethyl-2-thiazolyl] -2,5-diphenyl-2H-tetrazolium bromide; 5 mg/mL; (Sigma-Aldrich)] was added in each well and the plate incubated for 3 hours at 25°C ...
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bioRxiv - Bioengineering 2023Quote: ... blocked for 2 hr at room temperature in PBSTX (DPBS + 0.1% Triton X-100) containing 5% donkey serum (D9663, Sigma), incubated in primary and then secondary antibodies in blocking buffer for at least 48 hr at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... blocked for 2 hr at room temperature in PBSTX (DPBS + 0.1% Triton X-100) containing 5% donkey serum (D9663, Sigma), incubated in primary and then secondary antibodies in blocking buffer for at least 48 hr at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked for 2 hr at room temperature in PBSTX (DPBS + 0.1% Triton X-100) containing 5% donkey serum (D9663, Sigma), incubated in primary and then secondary antibodies in blocking buffer for at least 48 hr at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... consisting of 1% FBS and gluMax along with 5 μM CHIR99021 and 2 μM retinoic acid (R2625, Sigma-Aldrich) for 72 hrs ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... media was aspirated from cells and replaced with 5 mg/mL 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT, #M5655, Sigma Aldrich) solution in base media ...
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bioRxiv - Neuroscience 2024Quote: ... and then in 0.175 M sodium acetate (14.36 mg/ml in ddH2O, pH 6.8, adjusted with glacial acetic acid, 2 × 5 min; Cat# S8750, Sigma-Aldrich). Sections were reacted in 0.5 mg/ml 3 ...
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bioRxiv - Genetics 2022Quote: ... 5’-fluoroorotic acid (5’FOA; Sigma) was used at a concentration of 1 g/L in PMG containing 45 mg/L uracil ...
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bioRxiv - Cancer Biology 2020Quote: ... the target cells were trypsinized and replated in 6-cm dishes in media containing 2 μg/mL Puromycin (P8833, Sigma-Aldrich Quimica S.A., Madrid, Spain). Selection media was changed every 48-72 h for a minimum of 1 week before cell lines were frozen in 50% FBS and 10% DMSO and stored in liquid nitrogen for later use.
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bioRxiv - Neuroscience 2023Quote: ... 50 mM 6-OHDA exposure were made from a stock solution of 100 mM 6-OHDA (Sigma-Aldrich) in 20 mM L-Ascorbic Acid (AA) ...
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bioRxiv - Cancer Biology 2024Quote: ... (2) 2% Alconox (Sigma 242985 ...
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bioRxiv - Cancer Biology 2023Quote: ... (2) 2% Alconox (Sigma, 242985 ...