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Citations for Millipore Sigma :
2601 - 2650 of 10000+ citations for 6 Hydroxy 2 4 5 triaminopyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: 8 to 15-weeks old female C57BL/6j CD45.2+ mice were inoculated subcutaneously with 5×105 B16-F10-OVA and conditioned 4 days later with peritoneal injection of 300 mg/kg cyclophosphamide (Sigma). On day 7 ...
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bioRxiv - Systems Biology 2023Quote: ... All membranes were blocked in a 5% milk solution and then incubated for 12h at 4°C with either the mouse anti-phosphotyrosine 4G10 (05321, Millipore) or the rabbit anti-GFP (Thermo A11122 ...
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bioRxiv - Biophysics 2023Quote: ... PIEZO1 and Gardos ion channels were inhibited with 5 µM GsMTx-4 (Alomone Labs) and 10 µM TRAM34 (Sigma-Aldrich), respectively ...
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bioRxiv - Microbiology 2023Quote: ... the blots were washed again using TBST and developed using AP-reactive Nitrobluetetrazolium (NBT) 5-bromo-4-chloro-3-indolylphosphate (BCIP) tablets (Sigma). A final image of the blots was taken on a Bio-Rad ChemiDoc gel imaging system using colorimetric detection.
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bioRxiv - Microbiology 2023Quote: ... The insoluble matter was removed by centrifugation 10000×g for 5 min at 4 °C and protein was estimated using BCA kit (Sigma) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... The solubilized nucleoproteins were cleared by centrifugation at 20,400xg for 5 min at 4°C followed by filtration using an Ultrafree-MC spin column (Millipore, UFC30GV0S).
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bioRxiv - Biophysics 2023Quote: ... product of fusion protein Bre1(591-700) -(GGS)4- Bre1(591-700) -(GGS)5-Rad6(1-172) was cloned into vector pET32-a (Novagen) with N-terminal thioredoxin ...
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bioRxiv - Developmental Biology 2023Quote: ... and then incubated overnight at 4 ℃ with primary antibodies diluted in 5% goat serum in PBS: phospho-histone H3 Ser10 (Millipore, Burlington ...
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bioRxiv - Cell Biology 2023Quote: ... 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) and the AhR inhibitor CH-223191 were purchased from Millipore Sigma (St. Louis, MO). Indole-3-acetate sodium salt (I3A ...
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bioRxiv - Biochemistry 2024Quote: ... The protein expression of the mouse or human 5-HT3A was induced by the addition of 4 μg/mL tetracycline (Sigma). Valproic acid (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... for 5 minutes and washed 3-4 times in PBS before being mounted in Mowiol mounting media (Millipore, 475904-M).
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bioRxiv - Cell Biology 2024Quote: ... whole seedlings were ground in Eppendorf tubes using a pellet pestle and total proteins were extracted in SDS extraction buffer (40 mM Tris-HCl pH 7.5, 10% glycerol, 5 mM MgCl2, 4% SDS, 1x protease inhibitor cocktail (Sigma-Aldrich), 1 mM phenylmethanesulfonyl fluoride) ...
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bioRxiv - Microbiology 2024Quote: ... 4 °C), resuspended in 5 ml lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5ξ CellLytic B (Sigma-Aldrich), 1 % Triton X 100 ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were resuspended in 0.4 ml of sonication buffer (20 mM Tris pH 8.0, 5 mM EDTA, 1x EDTA-free protease inhibitors [Sigma Aldrich]) and subjected to sonication at 4.5-micron amplitude for 7 cycles of 15 s on / 15 s off ...
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bioRxiv - Cancer Biology 2024Quote: ... ∼2 to 5 μg genomic DNA in 50 μL of HPLC grade water was digested with 4 Units of Nuclease P1 (Sigma) at 65°C for 10 minutes in a digestion buffer containing 0.04 mM desferrioxamine mesylate (DFAM) ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were cultivated under humidified conditions with 5% CO2 at 37°C and passaged every 3-4 days using 0.05% trypsin/EDTA (Sigma-Aldrich). Cells were harvested in ice-cold PBS buffer using a cell scraper ...
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bioRxiv - Immunology 2024Quote: ... at 37°C in pre-warmed epithelial cell removal solution (HBSS supplemented with 4% iHS [Sigma] and 5 mM EDTA [Sigma]). The remaining tissues containing lamina propria and muscularis layer were digested for 20 min digestion ...
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bioRxiv - Neuroscience 2021Quote: ... The left MFB was injected with either 1μL of 6-Hydroxydopamine hydrochloride (6-OHDA, (Sigma-Aldrich) 3.2µg/µL free-base in a 0.02% ascorbic acid solution (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: Serum IL-6 levels were quantified using a commercial mouse IL-6 ELISA kit (RAB0308, Sigma) according to the manufacturer’s instructions and analyzed using spectra-iMAX (Molecular Devices).
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bioRxiv - Cell Biology 2021Quote: ... 6 mM ATP or 6 mM ADP (conjugated with sodium salt; Sigma-Aldrich, St. Louis MO) were included along with the contents described above ...
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bioRxiv - Neuroscience 2021Quote: ... cultured neurons from floxed conditional AP-2 KO mice expressing a tamoxifen-inducible Cre recombinase were treated with 0.25 μM (Z)-4-hydroxytamoxifen (Sigma) at DIV3 ...
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bioRxiv - Molecular Biology 2020Quote: ... then 10min at 4°C in the Lysis buffer 2 (50mM Tris pH8, 10mM EDTA, 0.5% NP-40, Complete protease inhibitor (Sigma)) and subsequently sonicated in 15ml conical tubes with a Bioruptor Pico (Diagenode ...
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bioRxiv - Immunology 2022Quote: ... for 2 or 4 days in complete culture medium composed of RPMI supplemented with 10% foetal calf serum (Sigma), 0.05mM 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2019Quote: ... and 10 μM biotin-tubulin were incubated in BRB80 (80 mM PIPES pH 6.85, 2 mM MgCl2, 1 mM EGTA) with 4 mM GTP (Sigma) for 25 min at 37°C in a total volume of 25 ul ...
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bioRxiv - Neuroscience 2020Quote: ... They were cut into 250μm parasagittal slices using a McIlwain tissue chopper and the slices placed on Millicell membrane (3-4 slices per membrane, 2 membranes per animal, 0.4 μm Millicell, Merck Millipore) in 50% BME (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were cleared by centrifugation and incubated at 4°C for 1.5–2 h with anti-FLAG M2 affinity gel (Sigma). After extensive washing with native IP buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... Primary acinar cells were pelleted (80 g, 2 min, at 4 °C) and re-suspended in Waymouth media (Sigma). To obtain acinar-cell-conditioned media ...
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bioRxiv - Neuroscience 2020Quote: (T-4)-[(1E,6E)-1,7-Bis[4-(dimethylamino)phenyl]-1,6-heptadiene-3’5-dionato-kO3’kO5] difluoroboron (CRANAD-2) [26] was purchased from Sigma-Aldrich AG ...
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bioRxiv - Microbiology 2021Quote: ... and the reaction was started with 100 μl of 4 mg/ml 2-nitrophenyl β-galactopyranoside (ONPG, Sigma-Aldritch) in Z buffer and stopped with 250 μl of 1M Na2CO3 ...
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bioRxiv - Neuroscience 2020Quote: ... 2-4 day old adult males raised in normal cornmeal food were transferred to food containing RU486 (mifepristone, Sigma) in 80% ethanol to a final concentration 200 μg/ml or with the same amount of ethanol (vehicle ...
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bioRxiv - Cell Biology 2020Quote: ... on ice and in a cold room at 4°C for 2 hours then warmed up to 37°C in complete medium with 10μM Nocodazole (M1404, Sigma) and incubated until fixation ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared by centrifugation at 16,000 xg at 4°C for 2 min and Pah1-FLAG was immunoprecipitated with anti-FLAG agarose beads (Sigma) at 4°C for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were incubated for 2 h on a rotating wheel at 4°C with anti-FLAG M2 beads (Sigma). Beads were washed once with lysis buffer (without benzonase ...
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bioRxiv - Cell Biology 2019Quote: ... #4 inguinal mammary glands were stained for several hours in carmine-alumn staining solution (2% w/v Carmine, Sigma; 5 % w/v Aluminium Potassium Sulfate ...
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bioRxiv - Neuroscience 2021Quote: Rat and mouse primary cortical neurons and BE(2)-M17 cells were fixed using 4% paraformaldehyde (PFA, Sigma-Aldrich) in PBS pH 7.4 for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for 2 days at 4°C in goat anti-calretinin (1:200; Cat#AB1550, EMD Millipore), followed by 2.5 hours incubation (RT ...
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bioRxiv - Cell Biology 2019Quote: ... were fully digested for 2 days at 4 °C in 0.1M of HCl digestion solution containing 1 mg/ml pepsin (Sigma). Collagen content was measured using the Sircol collagen assay kit (Biocolor) ...
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bioRxiv - Zoology 2020Quote: ... We used standard dilutions of hen egg white lysozyme (0.5, 0.8, 1, 2, 4, 8, 10, 20, and 40 µg/ml; Sigma-Aldrich) to prepare a standard curve in each plate ...
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bioRxiv - Cell Biology 2020Quote: ... MitoTEMPO ((2-(2,2,6,6-Tetramethylpiperidin-1-oxyl-4-ylamino)-2-oxoethyl)triphenylphosphonium chloride; #SML0737) and KCN (potassium cyanide; #60178) from Sigma-Aldrich; chemicals applied in Saccharomyces cerevisiae Meyen ex E.C ...
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bioRxiv - Microbiology 2022Quote: ... 20 adult female flies (2–4 days old) per vial were fed with 50ug/mL of RU486 (M8046, Sigma; stock solution is 10mg/mL in DMSO ...
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bioRxiv - Biochemistry 2022Quote: Phagosomal proteins from RAW264.7 cells were extracted in 1% sodium 3-[(2-methyl-2-undecyl-1,3-dioxolan-4-yl)methoxy]-1-propanesulfonate (commercially available as RapiGest, Waters) in 50 mM Tris (Sigma) pH 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... induction of DiCre activity when required was by treatment for 2–4 h with 100 nM rapamycin (RAP; Sigma) as described previously (Collins et al. ...
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bioRxiv - Cell Biology 2023Quote: ... specimens were prepared in PBS and subsequently fixed for 4 h at RT in fixative (2% glutaraldehyde (Sigma, Germany)/ 4% paraformaldehyde (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... Medium samples from day 2 and day 4 had to be diluted 1:1 in PBS (P4417, Sigma-Aldrich) because of the higher levels of cell death in the first days after harvesting caused by the isolation procedure ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated at 37 ℃ and 5% CO2 for 2 weeks and were stained with 500 µl of 4% formaldehyde and 0.005% crystal violet (C3886, Sigma) for 4 hours ...
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bioRxiv - Immunology 2023Quote: ... Serum and supernatant from homogenized tissues were incubated overnight at 4°C with 2% Trichloroacetic acid solution (Sigma, #T0699) at a 1:1 dilution ...
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bioRxiv - Genetics 2023Quote: ... The seminiferous tubules were incubated for 60 min at RT in fixation buffer (100 mM HEPES pH7.4, 4 mM CaCl2, 2.5% glutaraldehyde, 2% PFA, all from Sigma Aldrich) and then the buffer was exchanged with fresh fixation buffer and the samples left overnight fixed at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... 4°C) and resuspended in 1-1.5 ml chilled wash and resuspension buffer containing 2% Bovine Serum Albumin (Sigma) and 0.2 U/μl RNase Inhibitor (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... and the supernatant was incubated for 2 h at 4 °C with 150 μL streptavidin-agarose beads (Sigma-Aldrich) under gentle agitation ...
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bioRxiv - Immunology 2024Quote: ... and the bronchoalveolar lavage (BAL) fluid was collected in 0.5+0.6×4 ml 2% BSA (Sigma, St. Louis, MO). The supernatant of the initial 0.5 ml was stored for cytokine detection ...