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Citations for Millipore Sigma :
2651 - 2700 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 2-Deoxy-D-glucose (2-DG; 2 mM, Sigma-Aldrich).
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bioRxiv - Biochemistry 2024Quote: ... 2 mM 2-Mercaptoethanol (Sigma-Aldrich; Cas: 60-24-2), and 4 mM 4-Nitrophenyl acetate (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... and the GST tag was cleaved on column by incubating the protein with Thrombin (Sigma Aldrich) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: Antibodies against LeishIF4E2(rabbit polyclonal, 1:2,000) and against the SBP tag (Millipore, monoclonal, 1:10,000), were used to detect the endogenous and tagged LeishIF4E2 proteins ...
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bioRxiv - Microbiology 2019Quote: ... The hexa-histidine tag was removed overnight with thrombin (5 U thrombin (Sigma-Aldrich, Darmstadt, Germany) per mg SiiA protein ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were eluted by protease cleavage of the GST-tag (thrombin or PreScission (Sigma-Aldrich) were used depending on the enzyme availability ...
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bioRxiv - Biochemistry 2021Quote: Monoclonal antibodies against the Flag tag epitope and Cdh1 were purchased from Sigma (St. Louis, MO) while antibodies against GFP ...
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bioRxiv - Biophysics 2020Quote: ... MSP1D1 was purified using IMAC72 with further cleavage of 6xHis-tag by TEV protease (Sigma-Aldrich). The lipid mixture of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) ...
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bioRxiv - Molecular Biology 2022Quote: Full-length REC114 and its variants were cloned as Strep-tag fusions into pRSFDeut-1 (Novagen). TOPOVIBL 1-385 was cloned as a 6xHis-SUMO fusion protein in pETM11 ...
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bioRxiv - Biochemistry 2019Quote: ... The hexahistidine tag was removed by thrombin cleavage by incubating with 1:5000 thrombin (EMD Millipore) overnight at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... They included Fluidigm CS1 and CS2 tags and were ordered from Sigma (see appendix table 3). Reactions were performed in multiplex primer groups with primers each at 1 μM concentration (group 1 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Orphan GPCR expression was detected using rat anti-HA tag (clone 3F10) antibodies (Sigma-Aldrich; 11867423001) or rabbit anti-myc tag antibodies (GenScript ...
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bioRxiv - Cell Biology 2021Quote: The flag-tag immunoprecipitations were carried out using FLAG-M2 coated agarose beads (Sigma-Aldrich #F2426) for 2 hours at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Target proteins were detected using the following antibodies: mouse anti-Strep tag II (Millipore Sigma: 71590); rabbit anti-Caspase 3 (Cell signaling ...
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bioRxiv - Microbiology 2022Quote: ... followed by incubation with primary antibodies against Strep Tag II Antibody HRP conjugate (Sigma, 1:5000), anti-FLAG Antibody (Sigma F7425 ...
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bioRxiv - Microbiology 2024Quote: ... and incubated with a mouse monoclonal antibody [M2] specific to the FLAG-tag (Sigma-Aldrich, F1804) in 1% BSA/PBS at 37℃ for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... ORF3c-HA was detected using a primary antibody against the HA-tag (Sigma-Aldrich, Cat# H3663) followed by staining with a secondary AF555 antibody (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... HA tag sequence (YPYDVPDYA) was introduced by linker insertion at the 5’ side of pET28a (Novagen) MCS to generate pET28-HA-Nter ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-PA tag-PLUS PLA probe and anti-rabbit IgG-MINUS PLA probes (DUO92005: Sigma-Aldrich) appropriately diluted in the Duolink antibody diluent were applied onto the specimens that were incubated for 60 min at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... Flag-Tag (F1804, 1:2,000 for Western blot, 1:200 for immunofluorescence) was from Sigma-Aldrich. Anti-mouse IgG–HRP (7076S ...
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bioRxiv - Microbiology 2023Quote: ... Western blotting for the 6xHis-tag was performed using either HRP-conjugated primary antibody (Sigma, rabbit) or non-HRP-conjugated antisera (rabbit).
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bioRxiv - Synthetic Biology 2024Quote: ... to the ones with an mAID2 tag and 0.1μM TMP (Trimethoprim Ready Made Solution, Sigma Aldrich) to the ones with an ecDHFR tag right after transfection or at several time points.
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bioRxiv - Biochemistry 2019Quote: ... rabbit anti-flag (F7425,1:1000) and mouse anti-flag (F3165,1:1000) were from Sigma and rabbit anti-GFP (A11122,1:1000 ...
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bioRxiv - Developmental Biology 2024Quote: ... Spy650-FastAct(1:1000, SpiroChrome, SC505) and DAPI (1:1000, Sigma Aldrich, MBD0015-15ML) for 3 hours at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... of each IgG were prepared by using ~ 500 μl of antibody (mAb) (1-5 mg/ml) was mixed with 1200 μl of 100mM Sodium Acetate (sodium acetate anhydrous from Sigma Aldrich) having 1mM Ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA for electrophoresis ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit anti-BUBR1-p620 (custom raised by Moravian, 1:1000) and mouse anti-α-Tubulin (clone B-5-1-2, T5168, Sigma-Aldrich, 1:10000). Secondary antibodies used were goat anti-mouse IgG HRP conjugate (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: ... 10% fetal bovine serum in PBS) for 2 hours in room temperature and stained with anti α-Tubulin (DM1A; Sigma-Aldrich, 1:1000, overnight) followed by secondary antibody staining (Alexa Flour 488 anti-mouse 1:1000,2 hours in room temperature).
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated for at least 20 hours at room temperature with a combination of the following primary antibodies in PBS-TX with 2% NGS or NDS: guinea pig anti-VGLUT1 (1:1000; AB5905, Sigma-Aldrich, St. Louis, USA), guinea pig anti-VGLUT2 (1:20,000 ...
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bioRxiv - Biochemistry 2022Quote: ... Primary antibody were diluted in Blocking Buffer as described below and incubated with samples for 2 hours: γ-H2AX monoclonal mouse antibody JBW301 (Millipore Sigma, 1:1000). Samples were washed with Blocking Buffer twice for 5 minutes each then treated with secondary antibody diluted 1:200 for 2 hours ...
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bioRxiv - Cell Biology 2020Quote: ... and untagged Rab4aΔHVR (Figure 1) were expressed in Escherichia coli BL21(DE3) cells (Novagen) harboring the pET-41-based vectors constructed ...
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bioRxiv - Genetics 2021Quote: ... coli strains DH5α was used for plasmid maintenance and SM10 (Novagen, Hornsby Westfield, Australia) HB101 (pRK2103 ...
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bioRxiv - Biophysics 2019Quote: ... His8-3C-Flag-Δ16NARL6(Q73L) was expressed in Escherichia coli BL21(DE3) cells (Novagen) at 20°C overnight after induction with 1 mM isopropyl β-D-1-thiogalactopyranoside (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... coli 10129 and AMC-resistant isolates were grown on LB agar (Lennox) (Sigma, UK) for colony counting during MIC determination.
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bioRxiv - Microbiology 2020Quote: ... coli DS1 using the GenElute Bacterial Genome Extraction Kit (Sigma-Aldrich, St. Louis, USA) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... and stimulated with LPS (4 μg/ml) from Escherichia coli (055:B5; Sigma-Aldrich), CD154 (1 U/ml ...
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bioRxiv - Neuroscience 2020Quote: ... The reagents used in microglia treatment were: LPS (Sigma, L5293, Escherichia coli, 0111:B4); ATP (sigma A2383) ...
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bioRxiv - Microbiology 2020Quote: ... coli strains were grown in Luria-Bertani (LB) broth (Sigma-Aldrich, CAT No.: L3022) and agar (BD Difco™ ...
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bioRxiv - Microbiology 2020Quote: ... and alkaline phosphatase (from Escherichia coli) were purchased from Sigma-Aldrich (St. Louis, MO). Itraconazole (ITZ ...
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bioRxiv - Cell Biology 2022Quote: The recombinant GST-tagged Nanobody against GFP69 expressed in Escherichia coli Rosettagami (Novagen, 71351) was purified with Glutathione Sepharose 4B beads according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Macrophages were stimulated with 50 ng/ml lipopolysaccharides from Escherichia coli O111:B4 (Sigma) for 24 hours.
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bioRxiv - Immunology 2020Quote: Mice were intraperitoneally (i.p.) injected with LPS from Escherichia coli O55:B5 (L4005; Sigma). Blood was collected using EDTA-coated tubes at indicated time ...
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bioRxiv - Immunology 2022Quote: ... Lipopolysaccharide from Escherichia coli O123:B8 was purchased from Sigma-Aldrich (St. Louis, Missouri).
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bioRxiv - Neuroscience 2022Quote: ... mouse anti adenomatous polyposis coli antigen (APC; clone CC1; Millipore, OP80-100UG; 1:100). Secondary antibodies were FITC conjugated goat anti-mouse IgG (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... The expression of GFP-SGS3 protein was induced in Escherichia coli Rosetta (DE3) (Novagen) by adding 0.1 mM isopropyl β-d-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Systems Biology 2020Quote: ... coli BL21 (DE3) were constructed on the basis of pET16b (Novagen, Madison, WI, USA) and are presented in Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... coli strain BL21 and purified by glutathione-agarose affinity chromatography (Sigma-Aldrich Cat #G4510) and eluted using elution buffer (50mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... coli with OD value 0.5 was mixed with isopropanol (Sigma-Aldrich, St. Louis, MO) to achieve a final concentration of 70% v/v ...
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bioRxiv - Biochemistry 2019Quote: ... coli strains were grown at 37°C and cultured in LB medium (L3522, Sigma), supplemented with the indicated concentration of kanamycin (30 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: Human recombinant TNFα and LPS (from Escherichia coli O111:B4) were purchased from Sigma. VCAM-1 (sc-13160) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Escherichia coli (0111: B4) LPS was purchased from Sigma-Aldrich (St. Louis, MO, USA). Ultra-pure water was produced by a Millipore A10 Milli-Q water purification system (Millipore ...