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Citations for Millipore Sigma :
2851 - 2900 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... DAPI (Sigma, D9542; 1:1000) was used for nucleic staining.
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bioRxiv - Molecular Biology 2023Quote: ... anti-FLAG (1:1000, Sigma) and anti-Tubulin (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Vinculin (Sigma, V4505, 1:1000).
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bioRxiv - Molecular Biology 2023Quote: ... anti-gH2A.X (1:1000, Millipore), anti-FK2 (&:250 ...
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bioRxiv - Cancer Biology 2023Quote: ... actin (Millipore MAB1501, 1:1000), N-Cadherin (CST 13116T ...
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bioRxiv - Cell Biology 2023Quote: ... (1:1000, Sigma-Aldrich T6793), anti-S100-B ...
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bioRxiv - Cell Biology 2023Quote: ... FLAG (1:1000, Sigma F1804), Por1 (1:1000 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Nystatin (1000 units/L, Sigma), Cyclohexamide (50 mg/L ...
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bioRxiv - Cell Biology 2023Quote: ... RHOT1 (Sigma, HPA010687, 1:1000), Vinculin (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... and Hoechst (1:1000, Sigma) before being mounted for microscopy.
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bioRxiv - Cell Biology 2023Quote: ... MAPL (Sigma, HPA017681, 1:1000), VPS35 (Abnova ...
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bioRxiv - Cell Biology 2023Quote: ... anti-FLAG (Sigma 1:1000), anti-TANGO2 (Proteintech 27 846-1-AP 1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... DAPI (Sigma, D9542; 1:1000) was used for nucleic staining.
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bioRxiv - Developmental Biology 2024Quote: ... SOX2 (1/1000, Millipore: ab5603), PHOSPHO-HISTONE 3 (1/1000 ...
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bioRxiv - Microbiology 2024Quote: ... FLAG (SIGMA, F1804, 1:1000), or HA (BioLegend ...
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bioRxiv - Neuroscience 2024Quote: ... NeuN (1:1000, Millipore, rabbit), GAPDH (1:5000 ...
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bioRxiv - Cell Biology 2024Quote: ... DAPI (1:1000, Sigma Aldrich) dye was used to stain nuclei.
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bioRxiv - Cancer Biology 2024Quote: ... HA (1:1000, Sigma-Alrdich), FLAG (1:4000 ...
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bioRxiv - Neuroscience 2024Quote: ... Neurofilament (1:1000, AB1987, Millipore), and Synaptophysin (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... vGluT1 (1:1000, AB5905, Millipore), Neurofilament (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... MOG (MAB5680, 1:1000, Millipore), CNPase (#C5922 ...
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bioRxiv - Developmental Biology 2024Quote: ... Prox1 (1/1000, MAB5654, Millipore), Sox5 (1/500 ...
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bioRxiv - Neuroscience 2024Quote: ... PH3 (Millipore, 06-570,1/1000); pVimentin-ser55(MBL,D076-3S,1/500) ...
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bioRxiv - Neuroscience 2024Quote: ... β-tubulin III (Sigma, 1:1000), FOXG1 (Takara ...
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bioRxiv - Immunology 2021Quote: ... pTT3-HKU1 or pTT3-OC43 Spike were transfected into suspension-adapted 293T cells using 293 Free transfection reagent (EMD Millipore Cat# 72181) or PEI transfection reagent (PolySciences Inc ...
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bioRxiv - Immunology 2021Quote: ... For RBD inoculations eight-week-old male BALB/c mice were immunized with 7.5 μg of the receptor binding domain of Spike protein (kindly gifted by Andrea Gamarnik, Argentina) in 75 μL Complete Freund’s Adjuvant (CFA, Sigma, St. Louis, MO) via subcutaneous injection and boosted 2 weeks later with 5 μg of RBD in 100 μL Incomplete Freund’s Adjuvant (IFA ...
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bioRxiv - Biochemistry 2020Quote: ... Clarified lysate was loaded onto a gravity column containing 1 mL HIS-Select Nickel Affinity Gel (Sigma) pre-equilibrated with Resuspension Buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2×YT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...
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bioRxiv - Cell Biology 2019Quote: ... blotted onto nitrocellulose and the proteins were detected with an anti-His antibody (Sigma-Aldrich, Steinheim, Germany). To test whether 6His-PBBem1 and Cdc11 can bind simultaneously to Cdc24428-854 ...
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bioRxiv - Biochemistry 2019Quote: ... Lysates were then added directly to 96-well His-Select Ni-NTA resin filter plates (Sigma Aldrich) and processed off-robot by centrifugation ...
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bioRxiv - Biochemistry 2021Quote: Genes for His-tagged or Strep II-tagged nanobodies were cloned into the pET 26b vector (Novagen). The expression and purification of all His-tagged nanobodies have been described previously 12 ...
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bioRxiv - Microbiology 2020Quote: ... and the supernatants were independently applied to a column of HIS-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with 50 mM sodium phosphate buffer ...
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bioRxiv - Biochemistry 2022Quote: ... and another was loaded with 10 ng of human HSP70 (His tagged human HSP70, SRP5190, Millipore Sigma), which allowed comparisons to be made among separate gels ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were detected using the primary antibody (Mouse α-His at 1:2500 dilution, Sigma Aldrich) followed by the HRP conjugated secondary antibody (Sheep α-Mouse at 1:7000 dilution ...
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bioRxiv - Biophysics 2020Quote: ... and passed through pre-equilibrated HIS-Select Ni-nitrilotriacetic acid resin (Ni-NTA) (Sigma-Aldrich Co., USA) at 4 °C for binding of 6x-His tagged protein ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used: anti-His (1: 2000, Cat No: 70796-4, Novagen, Madison, WI); anti-DNALI1 (1 ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... plasmid coding for 6x-His-Cas9 were transformed in Rosetta DE3 Novagen competent cells (Merck Millipore, USA). Protein production was induced using autoinduction medium (Formedium ...
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bioRxiv - Cell Biology 2020Quote: ... in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich, #U5507), and ≈ 2 μg of purified recombinant mouse GST-Trim39 (WT ...
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bioRxiv - Biophysics 2021Quote: ... The His-eluent was concentrated using a 30 kDa cutoff Amicon Ultra-4 centrifugal filter (Millipore UFC803008), filtered using a 0.22 μm Millex-GP PES membrane (Millipore SLGP033RS) ...
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bioRxiv - Biochemistry 2023Quote: ... After several rounds of washing with His-AC washing buffer (supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2xYT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...
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bioRxiv - Genetics 2023Quote: ... imidazole was removed from the eluted His-SIRT5 using ultra-0.5 centrifugal filter units (UFC501096, Merck Millipore). 500 µl of the recombinant SIRT5 elution fractionation was added to the ultra-0.5 centrifugal filter units and centrifuged at 14,000 x g for 15 min at room temperature ...
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bioRxiv - Systems Biology 2024Quote: ... Samples were stored at –20 °C and insulin secretion was quantified using human (Millipore Cat. # HI-14K) insulin radioimmunoassay kits.
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-phospho-c-Jun (Thr91, cell signal, 2303, 1:1000), rabbit anti-c-Jun (cell signal, 9165, 1:1000) and mouse anti-GAPDH (Sigma, Cat: G8795, 1:1000). Immunoreactivity was visualized using appropriate HRP-conjugated secondary antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... Myc (CST 71D10, 1:1000), p-EGFR Y1068 (CST 3777S, 1:1000), EGFR (CST 4267, 1:1000) and β-actin (Sigma A1978, 1:10000). Densitometry analysis was performed with ImageJ software (NIH).
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bioRxiv - Cell Biology 2022Quote: ... 2-hydroxy-4’-(2- hydroxyethoxy)-2-methylpropiophenone (I2959) (Sigma-Aldrich, USA), a photoinitiatior (0.5% w/v ...
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bioRxiv - Molecular Biology 2020Quote: ... 2% (v/v) 2-mercaptoethanol and 2 mM PMSF) (Sigma-Aldrich) and homogenize ...
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bioRxiv - Neuroscience 2020Quote: Animals were anesthetized with avertin (2, 2, 2-Tribromoethanol (Sigma-Aldrich), 125-250 mg/kg ...
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bioRxiv - Cell Biology 2022Quote: ... 2-Aminoethyl diphenylborinate (2-APB; Sigma), caffeine (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... 2-Deoxyglucose (2-DG, Sigma #D6134) was administered via IP injection at 0.5 mg/g bodyweight ...