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Citations for Millipore Sigma :
2901 - 2950 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Microplastic consumption induces inflammatory signatures in the colon and prolongs a viral arthritisbioRxiv - Immunology 2021Quote: ... embedded in paraffin and sections stained with hematoxylin and eosin (H & E; Sigma-Aldrich, Darmstadt, Germany). For immunohistochemistry ...
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bioRxiv - Developmental Biology 2021Quote: H&E staining on paraffin sections was performed following the producer recommendations (Sigma, Procedure No. HHS).
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bioRxiv - Microbiology 2019Quote: ... sectioned with a microtome (5 µm) and stained with hematoxylin and eosin (H&E; Sigma, USA) for histological examination [23] ...
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bioRxiv - Cell Biology 2019Quote: ... These parasites were immediately resuspended in media plus 7.5mM GlcN and 20 µM E-64 (Sigma) and incubated at 37°C in a still incubator for 6hrs ...
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bioRxiv - Physiology 2021Quote: ... and plated in 6-well plates (0.25×106 cells per well) in William’s Medium E (Sigma) supplemented with antibiotics and 5% fetal bovine serum (FBS ...
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bioRxiv - Plant Biology 2020Quote: ... as a nitrogen source and supplemented with 0.1% sucrose and 1% agar (Type E, Sigma A4675), pH=5,8 ...
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bioRxiv - Developmental Biology 2020Quote: PLAT-E cells and (where stated) iPSCs were cultured in GMEM basal medium (GMEM (Sigma-Aldrich), 1xNEAA (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... DNA content was assessed using a commercial kit: DNA quantitation kit e Fluorescence assay (Sigma Aldrich) in a 96-well plate ...
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bioRxiv - Biochemistry 2021Quote: Proteinase K (E. Album) and ammonium sulfate were purchased from Sigma (Sigma-Aldrich, St. Louis, MO) and used without further purification ...
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bioRxiv - Cell Biology 2022Quote: ... The schizonts were resuspended in 40 ml fresh complete RPMI with 10 μM E-64 (Sigma), a cysteine protease inhibitor that prevents merozoite release from schizonts by inhibiting rupture ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T-ACE2-TMPRSS2 cells were pretreated with indicated concentrations of EST/E-64D (E64d) (Sigma, 330005) or camostat mesylate (Camostat ...
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bioRxiv - Microbiology 2022Quote: ... Vero-ACE2-TMPRSS2 cells were pretreated with indicated concentrations of EST/E-64D (E64d) (Sigma, 330005) or camostat mesylate (Camostat ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... Hematoxylin & Eosin (H&E) staining of pancreatic sections were performed according to the manufacturer’s protocol (Sigma). The islet area was quantified using the same using ImageJ software.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... For hematoxylin/eosin (H&E) staining slides were deparaffinized as above followed by hematoxylin (Sigma, GHS232) solution for 10 minutes followed tap water for 15 minutes and eosin (Merck ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... For the preparation of the e-liquids were used propylene glycol (PG) (Sigma Aldrich, P4347-500ML), vegetable glycerol (VG ...
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bioRxiv - Cell Biology 2023Quote: ... and the suspension was seeded into a Poly-D-lysine (PDL; Sigma-Aldrich, A-003-E)-pre-coated 75 cm2- cell culture flask containing 10 ml of the supplemented DMEM ...
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bioRxiv - Microbiology 2023Quote: ... while the experimental group received oral administration of vitamin E (Sigma-Aldrich; Burlington, MA, USA; 258024) (500 mg/kg of body weight ...
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bioRxiv - Cell Biology 2023Quote: ... Degradation of the endogenous CENP-E is achieved through addition of 500 μM IAA (Sigma-Aldrich) (Owa & Dynlacht ...
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bioRxiv - Microbiology 2023Quote: ... blood was collected from healthy volunteers by venipuncture and mixed with K3EDTA (#E-0270, Sigma-Aldrich). The donors were two females and one male ...
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bioRxiv - Cancer Biology 2023Quote: ... the pellet was resuspended in William’s E Medium and layered onto Percoll® solution (Sigma-Aldrich) (10ml Percoll®+ 1.25ml PBS 10X + 1.25 ml H2O. ...
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bioRxiv - Cell Biology 2024Quote: ... The primary antibodies were used as follows: rabbit anti-CENP-E (1:100, C7488; Sigma-Aldrich), rabbit anti-Spindly/CCD99 (1:1000 ...
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bioRxiv - Pathology 2024Quote: ... p-RAPTOR (#, 1:1000), RAPTOR (#, 1:1000) or b-actin (#A5316, Sigma-Aldrich, 1:5000). Blots were revealed on a Chemidoc imager (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... and pcDNA5/FRT/TO Myc SARS CoV2 N and N 2A was transfected into HEK293T cells using GeneJuice (Novagen, Darmstadt, Germany) following the manufacturer’s protocol (12 μg of DNA/sample in total) ...
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bioRxiv - Immunology 2022Quote: ... The sections were then incubated overnight with primary antibodies directed against SARS Nucleocapsid protein (1/500; mouse monoclonal; clone 1C7C7; Sigma-Aldrich), ionised calcium-binding adapter molecule 1 (Iba1 ...
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bioRxiv - Bioengineering 2021Quote: ... was used to stain the sections for EdU and 4′,6-diamidino-2-phenylindole (DAPI; #D9542; 1:1000 dilution; Sigma-Aldrich, St. Louis, MO, USA). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... This was followed by a 2-hour incubation in an antibody mixture composed of a 1:1000 dilution of anti-digoxigenin-POD (Roche/Sigma-Aldrich, St. Louis, MO, USA) and a 1:500 dilution of anti-GFP antibody (ab6556 ...
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bioRxiv - Bioengineering 2024Quote: ... BSA (1%) and PBS-T (0.5%) followed by counterstaining with 4’,6-diamidino-2-phenylindole (DAPI) in PBS (1x) (Sigma-Aldrich, St. Louis, MO, 1:1000) for 20 minutes at RT ...
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bioRxiv - Neuroscience 2021Quote: ... which has the hSyn promoter followed by Flpo with 3×FLAG C-terminal tag (OGS629, Sigma-Aldrich). This plasmid was verified by sequencing and used throughout the study ...
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bioRxiv - Microbiology 2021Quote: Poly-histidine tags were cleaved from proteins by treating them with 3 units of TEV protease (Sigma) overnight at 4 °C based on the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... 8 µg antibody (Monoclonal ANTI-FLAG® M2, Art. No. F3165, Sigma-Aldrich® / Myc-Tag (9B11) Mouse mAb ...
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bioRxiv - Biochemistry 2022Quote: ... the GST-tag of GFP-N-WASP-VCA and cortactin was cut by PreScission Protease (Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibodies against FLAG tag [M2] (F1804, RRID: AB_262044) and [M2] (F3145, RRID:AB_259529) were purchased from Sigma Aldrich. Antibodies against DDX3X [D19B4] (8192S ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated in phosphate-buffered saline (PBS) containing 1/400 anti Rho-tag antibody 4D2 (Millipore), 15mM NaN3 ...
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bioRxiv - Molecular Biology 2019Quote: ... and the eluate was analysed by SDS-PAGE and western blotting with anti-His6-tag antibody (Sigma).
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bioRxiv - Microbiology 2020Quote: ... the mFc and 8xHis tags at the C-terminus were removed by HRV-3C protease (Millipore-Sigma) and then purified using Ni-NTA resin (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... the mFc and 8xHis tags at the C-terminus were removed by HRV-3C protease (Millipore-Sigma) and then purified using Ni-NTA resin (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: ... respectively) was cloned into a pRSF-Duet vector preceded by an N-terminal His6-SUMO tag (Novagen)[11 ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunoblots from tandem affinity purifications were probed with antibodies against S-tag (mouse monoclonal MAC112; EMD Millipore) followed by visualization using IRDye-tagged secondary antibodies.
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bioRxiv - Molecular Biology 2021Quote: ... The upper portion of the membrane was probed with anti-HA tag antibody (mouse, Sigma-Aldrich, H3663) diluted 1:1,000 in 5% skim milk solution ...
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bioRxiv - Biochemistry 2020Quote: ... Phage with uncleaved FLAG-tag were removed by immunodepletion using M2 anti-FLAG monoclonal antibody (Sigma-Aldrich) coupled to epoxy-activated M-450 magnetic beads (Invitrogen) ...
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bioRxiv - Physiology 2022Quote: TAG and cholesterol evaluation of tissue and serum were processed according to the manufactures’ instructions (Sigma-Aldrich, MAK266 for TAG and Cell Biolabs ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membranes were probed with antibodies to detect FLAG tag (Millipore-Sigma, Burlington, MA cat# F1804-200UG), histone H3 (Millipore-Sigma ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membranes were probed with antibodies to detect FLAG tag (Millipore-Sigma, Burlington, MA cat# F1804-200UG), histone H3 (Millipore-Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... The T7 expression vector pET21b with C-terminal 6xHis tag was originally purchased from Novagen (NdeI-XhoI). 5hmdCTP/dGTP/dATP/dTTP mix was purchased from Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... The T7 expression vector pET21b with C-terminal 6xHis tag was originally purchased from Novagen (NdeI-XhoI). E ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was cleaved from the GST tag by incubating the beads with human thrombin (Sigma-Aldrich) in the presence of 50 µM CaCl2 overnight at 4 °C with rocking ...
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bioRxiv - Biochemistry 2024Quote: ... GFP variants were cloned downstream of a 7xHis-SUMO tag in a modified pET22b vector (EMD Millipore). Tag variants were generated by ligating synthetic dsDNA oligos (IDT ...
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bioRxiv - Immunology 2024Quote: ... After enzymatic digestion (Collagenase XI 1200 U/mg, Collagenase I 125 U/mg, Hyaluronidase 500 U/mg, DNase I 1836 U/mg; Sigma Aldrich®) for 30 min at 37°C cells were washed and incubated with purified anti-CD16/32 (FcγRIII/II ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were incubated in secondary antibody (Alexa Fluor 488 Goat anti-Rabbit, 1:500, Invitrogen #11008; DAPI, 1:1200, Sigma- Aldrich #D9564) at 4°C in the dark overnight with rocking ...
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bioRxiv - Neuroscience 2024Quote: ... anti-calbindin (Swant, CB38, rabbit polyclonal, dilution 1:1000), anti-calretinin (Swant, 7697, rabbit, dilution 1:1000, anti-choline acetyl-transferase (Millipore, 2843074, goat, dilution 1:1000), anti-GABA (Sigma ...