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Citations for Millipore Sigma :
2401 - 2450 of 10000+ citations for 6 Hydroxy 2 4 5 triaminopyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Isolated MLECs were treated with increasing concentration (0.1, 5 and 50 μM) of 4-hydroxytamoxifen (OH-tamoxifen) (Millipore-Sigma) for 24 h ...
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bioRxiv - Systems Biology 2023Quote: ... Each cage was provided with a 30 ml glass bottle filled with a solution of 5% glucose and 0.4% methylparaben (methyl 4-hydroxybenzoate, Sigma). A vertically rolled up Whatman filter paper was inserted to soak up the sugar solution and allow mosquitoes to feed on.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and then incubated overnight at 4°C with primary antibodies diluted in blocking solution [5% Goat Serum (Sigma Aldrich) + 0.2% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Excess TMT reagent was quenched by the addition of 4 µl of an aqueous 5% hydroxylamine solution (Sigma, #438227). Peptides were reconstituted in 0.1 % formic acid and mixed to achieve a 1:1 ratio across all TMT-channels ...
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bioRxiv - Cell Biology 2023Quote: ... Excess TMT reagent was quenched by the addition of 4 µl of an aqueous 5% hydroxylamine solution (Sigma, 438227). Peptides were reconstituted in 0.1 % formic acid and equal volumes were mixed ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was cleared by centrifugation at 16,000 g for 5 min at 4 °C before it was immobilized on anti-FLAG (Sigma) agarose bead slurry for 30 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... and digested in 4 mL of RPMI-1640/5% HI-FBS containing 1 mg/mL collagenase D (Sigma-Aldrich) and 30 μg/mL DNase I (Sigma ...
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bioRxiv - Immunology 2023Quote: Male mice were sensitized with 4 mg of PN butter (∼1 mg of protein; Capitán Mani) and 5 μg of cholera toxin (C8052, Sigma) in 0.5 mL of PBS administered intragastrically ...
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bioRxiv - Plant Biology 2023Quote: ... Spores were grown on solid 0.5× Gamborg B-5 media for 5 days and dispensed in 6-well plates containing 4 mL of liquid 0.5× Gamborg B-5 plus supplements: 0.1% N-Z amino A (Sigma #C7290) 0.03% (w/v ...
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bioRxiv - Developmental Biology 2023Quote: Membranes were incubated overnight in primary antibody o/n at 4°C: Rabbit ⍺-Vang (Rawls & Wolff, 2003) diluted 1:1,000 in 5% milk in TBST or Rabbit ⍺-Actin (Sigma) diluted 1:2,000 in 10% Seablock in TBST followed by 3x wash in TBST for 10 minutes each ...
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bioRxiv - Genomics 2023Quote: Undifferentiated shCTRL and shCPOX-4 Cells seeded at 0.5M cells/ml and treated with 5 μl 10mM GSK343 (Sigma, catalog no ...
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bioRxiv - Cell Biology 2023Quote: ... gel samples were prepared by boiling for 5 min in 2x SDS sample buffer (125 mM Tris, 4% SDS, 20% glycerol, 0.04% bromophenol blue, pH 6.78) supplemented with 5% β-mercaptoethanol (Sigma). Samples were briefly centrifuged prior to loading onto 4-12% NuPAGE Bis-Tris mini-gels (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Beads were re-suspended in antibody buffer (50 μL) with BG4 (∼5 μM, 4 μL/sample) or H3K27ac antibody (0.5 μL/sample, Millipore) and incubated at 4 ◦C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... 5 minutes each) prior to incubating in blocking solution (4% BSA in PBS with 0.33% Tween-20, Sigma-Aldrich) at room temperature for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei pellets were then collected by centrifuge for 4 min at 1,300 × g at 4°C and washed once in PBS supplemented with 5 mM sodium butyrate (Sigma) before binding to prepared concanavalin A coated magnetic beads (Bangs Laboratories ...
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bioRxiv - Physiology 2024Quote: ... WT and Gw females (4 months of age) underwent superovulation by intraperitoneal (IP) injection of 5 IU pregnant mare’s serum gonadotrophin (PMSG; Sigma Cat.# G4877-1000 IU ...
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bioRxiv - Developmental Biology 2024Quote: ... at 4 x 105 cells/well on 24-well plate (Trueline) coated with 5 μg/ml fibronectin (Sigma-Aldrich). Cells were treated with either 0.01% DMSO or different concentrations of Ki8751 inhibitor for 6 hours ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 10 μl of 4 mg/ml 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB, Sigma-Aldrich, St. Louis, MO, USA) in reaction buffer pH 8 was added to wells containing samples and standards ...
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bioRxiv - Cell Biology 2019Quote: ... and collected by centrifugation at 400 g for 2 min before fixation with 4% Paraformaldehyde (PFA, Sigma-Aldrich) at room temperature for 10 min ...
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bioRxiv - Cell Biology 2021Quote: SARS-CoV-2-infected and mock-treated hiPSC-CMs were fixed using 4% paraformaldehyde solution (Sigma-Aldrich, EUA) for 1 hour and stored at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... 6–8-week-old C57BL/6N mice were anesthetized by inhalation of isoflurane (3–4%) or intraperitoneal injection of 2% Avertin solution (2,2,2-tribromoethyl alcohol dissolved in Tert-amylalcohol (Sigma)) dissolved in saline ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed with 4% paraformaldehyde and stained with a 2% Oil Red Sudan lipid 5B (Sigma Aldrich) for 10 min at different time points to quantify differentiated cells containing red-colored lipid vacuoles.
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bioRxiv - Molecular Biology 2022Quote: ... induction of DiCre activity when required was by treatment for 2–4 h with 100 nM RAP (Sigma) as described previously (32 ...
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bioRxiv - Cancer Biology 2019Quote: ... 4 µl of SUV TBS solution (1:1 ratio of SUV and 2× TBS solution, TBS form Sigma) was placed in each hole of the device ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was incubated for 1.5-2 h at 4 °C with Anti-FLAG M2 affinity gel (Sigma) while gently mixing ...
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bioRxiv - Bioengineering 2019Quote: ... iPSC-CM were cryopreserved at 2-4 E7 cells/mL in growth media supplemented with 20% dimethylsulfoxide (Sigma).
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bioRxiv - Microbiology 2019Quote: ... at 4°C and resuspended in 2 ml of NP40 lysis buffer (1× CellLytic B (Sigma, MO, USA), 50 U DNAse I (Fermentas ...
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bioRxiv - Physiology 2021Quote: ... differentiated Caco-2 epithelial integrity was assayed by measuring passive diffusion of 4 kDa FITC-Dextran (Sigma Aldrich) added at a concentration of 5 μM to the apical chamber in 100 μL PBS ...
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bioRxiv - Physiology 2020Quote: ... primary neuronal cells isolated from control and Drp1AgRPKO mice were treated 2 µM 4-hydroxytamoxifen (H7904, Sigma-Aldrich) for expression of a CreER recombinase ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were incubated 2 minutes at 4°C with 0.1 mg/ml propidium iodide (Cat. P4170, Sigma Aldrich) and fluorescence pulses were detected using 585/40 nm collection filter ...
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bioRxiv - Biochemistry 2022Quote: ... β-mercaptoethanol and HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid] were procured from Sigma (St. Louis, MO, USA). Urea and proteinase-K were bought from Amresco ...
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bioRxiv - Physiology 2022Quote: ... a 4 uL suspension of 2 μm red-fluorescent polystyrene microspheres (Sigma-Aldrich; 1:1000 dilution in PBS) was apically applied to native mucus ...
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bioRxiv - Plant Biology 2022Quote: ... seeds were placed on 20 mL of 0.025% (w/v) Hyponex No.2 medium solidified with 4 mM CaCl2 and 0.25% phytagel (Sigma) in the test tube ...
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bioRxiv - Cell Biology 2024Quote: ... 2% homemade NS-21 (Chen et al., 2008) and 4 µM cytosine β-D-arabinofuranoside (Millipore Sigma C6645). Neurons were incubated at 37 °C in a 95% air/5% CO2 incubator until imaging.
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bioRxiv - Microbiology 2024Quote: ... The supernatant was removed and cell pellets were resuspended in 2 ml ice-cold 4% formaldehyde (Sigma, 47608) and incubated with shaking overnight at 4℃ ...
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bioRxiv - Biochemistry 2023Quote: ... was incubated for 2 hrs at 4 °C with Protein A/G Mix Magnetic Beads (Cat# LSKMAGAG10, Millipore). After washing with dilution buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Rie1-3V5 was IPed at 4°C 2 h using 20 µL of anti-V5-agarose slurry (Sigma). After incubation ...
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bioRxiv - Synthetic Biology 2023Quote: ... and a titration of trimethoprim (0, 1, 2, 4, 8, 16, 24, and 32 µg/mL) (Sigma Aldrich) with 0.4 % dimethylsulfoxide ...
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bioRxiv - Immunology 2023Quote: ... cells were spun onto microscope slides using a Cytospin 2 and fixed with 4% paraformaldehyde (PFA, Millipore- Sigma) for 20 minutes ...
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bioRxiv - Immunology 2023Quote: ... cells were spun onto microscope slides using a Cytospin 2 and fixed with 4% paraformaldehyde (PFA, Millipore- Sigma) for 20 minutes ...
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bioRxiv - Immunology 2023Quote: ... Surface staining was performed for 30 min at 4 °C in PBS supplemented with 2% FCS (Sigma-Aldrich) and 0.01% azide (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... for 2 hours at 4 °C and then incubated with anti-Flag M2 affinity gel (Millipore Sigma # A2220) for 1.5 hours at 4 °C under continuous rotation ...
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bioRxiv - Biochemistry 2023Quote: 2 mkL or 4 mkL of a 250 mM solution of GME (Sigma-Aldrich, St. Louis, MO, USA) in water was added to 50 mkL of cell suspension prepared as in section 2.1 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were fixed in 4% PFA for 2 min and stained with Alkaline Phosphatase Detection Kit (Sigma-Aldrich). Colonies were manually counted distinguishing between differentiated ...
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bioRxiv - Immunology 2024Quote: Larvae and juveniles at 2-24 days post fertilisation (dpf) were fixed in 4% paraformaldehyde (PFA) (Sigma; P6148) overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... nuclei were stained with DAPI (2 μg/ml, room temperature, 5 min, Sigma, St. Louis, Missouri, USA).
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bioRxiv - Plant Biology 2022Quote: ... DSF supplementation was performed by adding with 5 μM of cis-11-methyl-2-dodecenoic acid (Sigma) to the media.
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bioRxiv - Molecular Biology 2020Quote: ... 10% FCS and human fibroblast growth factor-2 (5 ng/mL, Sigma-Aldrich, St Louis, MO, USA) at 37 °C ...
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bioRxiv - Microbiology 2019Quote: ... yeasts were incubated in 1 mL of 5% 2-mercaptoethanol in SPM buffer (Sorbitol 1.2 M (Sigma), potassium phosphate monobasic 50 mM (Sigma) ...
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bioRxiv - Molecular Biology 2019Quote: Mouse monoclonal α-Flag (M2) and α-tubulin (clone B-5-1-2) were obtained from Sigma. Rabbit polyclonal α-HA (ab9110 ...