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Citations for Millipore Sigma :
2351 - 2400 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Either 10 or 20 ul of sample were separated by 12 % SDS-PAGE and transferred onto PVDF membrane (Millipore). After blocking with 5 % Milk powder (Roth ...
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bioRxiv - Physiology 2020Quote: ... Proteins were resolved on a 4%–12% SDS-PAGE gel and transferred to a PVDF membrane (Immobilon-FL, Millipore), incubated with indicated antibodies overnight at 4°C and subsequently quantitated with the LiCOR Odyssey instrument with Odyssey Image Studio software using 2 different infrared channels ...
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bioRxiv - Genetics 2020Quote: ... osteoblasts were seeded onto 18-mm glass coverslips in a 12-well plate and fixed with 4% formaldehyde (Sigma) the next day ...
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bioRxiv - Cancer Biology 2020Quote: ... IFNγ and TNFα production were assessed by flow cytometry following stimulation of 1×106 whole splenocytes with 0.1μg/ml of phorbol 12-myristate 13-acetate (PMA, Sigma P8139) and 1μg/ml of Ionomycin (Sigma I0634 ...
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bioRxiv - Cancer Biology 2020Quote: ... The mixture of sperm and Il11-Egfp DNA was diluted with Hepes-modified CZB containing 12% polyvinylpyrrolidone (Sigma-Aldrich) before being used for ICSI ...
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bioRxiv - Bioengineering 2020Quote: ... THP-1 cells were differentiated into macrophage-like cells with 150 nM phorbol 12-myristate 13-acetate (PMA) (Sigma) before the experiment.
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bioRxiv - Neuroscience 2020Quote: ... The dissociated DRG neurons were then plated onto poly-D-lysine coated 12 mm cover glass (Sigma-Aldrich, Australia). Neurons were left to attach for ~3 hours at 37°C after which media was added and then incubated overnight and used within 24 hrs.
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bioRxiv - Cell Biology 2021Quote: ... Lysates containing FLAG-tagged motors were diluted in assay buffer [AB: P12 buffer (12 mM PIPES (Sigma P-1851), 1 mM EGTA (Sigma E-4378) ...
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bioRxiv - Cell Biology 2021Quote: ... murine CD4+ T cells were isolated and activated in a 12 well plate with culture media containing 2g/L D-Glucose-1,2-13C2 (Sigma) for 24 hours ...
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bioRxiv - Bioengineering 2021Quote: ... The LCLs were maintained under aseptic conditions in a 1:1 mixture of Dulbecco’s modified Eagle medium and nutrient mixture F-12 (DMEM/F12; DF-041-B; Merck Millipore) supplemented with 1% antibiotic/antifungal (15240062 ...
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bioRxiv - Cell Biology 2021Quote: ... RageAHA/AHA and Rage−/− mice were fasted overnight (12 to 15 hours) to deplete GSH stores before APAP (Sigma) administration ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were separated via gel electrophoresis on 7.5-12 % polyacrylamide gel and transferred to a PVDF membrane (Sigma Aldrich) using a semi-dry blotting system ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were resolved on 4-12% Bis-Tris Gels (Thermo) or homemade 6% Bis-Tris gels and transferred to 0.45 μm PVDF (Millipore) membranes ...
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bioRxiv - Microbiology 2021Quote: ... Samples were kept in dark and cold conditions while prefiltered by gravity through 10 μm nylon mesh (KC Denmark) and then onto 8-12 47 mm 0.45 μm Durapore filters (Millipore) using a vacuum pump ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated by 12% SDS-polyacrylamide gel electrophoresis (PAGE) and transferred by electroblotting onto polyvinylidene difluoride membrane (Millipore). After blocking 1 h with 5% nonfat dry milk ...
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bioRxiv - Microbiology 2020Quote: ... the eluted proteins were loaded to 12% SDS-PAGE gel and then transferred to PVDF membranes (Millipore, Boston, USA). After blocking with 5% non-fat milk for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... THP-1 cells differentiated with 100 ng/mL phorbol-12-myristate 13-acetate (PMA) (Sigma-Aldrich, CAT No.:79346) were allowed to adhere for 24 hours before infection ...
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bioRxiv - Molecular Biology 2021Quote: ... cell media was discarded and each plate was washed with 12 ml of cold PBS 1X (D8537, Sigma-Aldrich). Cells were UV-crosslinked at 254 nm with 2000 J/m2 in ice and scrapped ...
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bioRxiv - Cancer Biology 2020Quote: The established murine colon carcinoma cell line MC38 (21) was maintained in Dulbecco’s Modified Eagle’s Medium Ham’s Nutrient Mixture F-12 (DMEM, Sigma-Aldrich, D8437) with 10% fetal bovine serum (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were cultured in Dulbecco’s modified Eagle’s medium/Ham F-12 (DMEM-F12) (Sigma Aldrich, St. Louis, MO, USA) supplemented with 40 ng/ml basic fibroblast growth factor-2 (FGF2 ...
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bioRxiv - Immunology 2021Quote: ... the eluted proteins were loaded onto a 12% SDS-PAGE gel and then transferred to a PVDF membrane (Millipore). After blocking with 5% nonfat milk for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... Chemotaxis plates are square plates containing 12 ml of modified NGM agar (without peptone and cholesterol, 1.7% Granulated, purified agar (Agar-agar, Millipore), 50 mM NaCl ...
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bioRxiv - Genetics 2022Quote: ... NSCs were washed with Wash Media (WM) (DMEM/Ham’s F-12 media with l-Glutamine (Sigma-Aldrich, D8437-500), 300 mM D-(+)-Glucose solution (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... RPE-1 cells were cultured in Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham with 15 mM HEPES and sodium bicarbonate (Sigma, D6421) supplemented with 6mM L-glutamine (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were seeded onto 12 mm glass coverslips pre-coated with 0.1 mg/ml poly-D-lysine (P0899, Sigma) at a density of 80000 cells/cm2 ...
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bioRxiv - Microbiology 2020Quote: ... mice were 7-12 week old C57BL/6J or Arhgef26-/- mice were fasted four hours before treatment with 20μg of streptomycin (Sigma) in 75μL of sterile water 24 hours before infection ...
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bioRxiv - Microbiology 2021Quote: ... J-Lat latent proviruses were reactivated by adding 10 µM of phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich) to the culture media for 18 hours ...
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bioRxiv - Cell Biology 2021Quote: Samples were run on 12% SDS-polyacrylamide gels and subsequently transferred to 0.2 μm polyvinylidene difluoride membrane (PVDF) (Millipore). Membranes were washed with 1X TBS and incubated with a 5% solution of BSA for 1 hr at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... THP-1s were plated on 8-well Nunc Lab-Tek II chamber slides (Thermo-Fisher) at a density of 150,000 cells per well with 100nM PMA (phorbol 12-myristate 13-acetate, Sigma) in growth media and incubated at 37°C and 5% CO2 for 24 h ...
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bioRxiv - Neuroscience 2020Quote: SH-SY5Y cells (DSMZ ACC 209) were maintained in DEMEM/F-12 (1:1) supplemented with 15% FBS (Sigma), 1% non-essential amino acid solution ...
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bioRxiv - Immunology 2020Quote: ... To induce monocyte to macrophage differentiation cells were plated at 0.4×106/mL) in complete media supplemented with 25 ng/mL phorbol 12-myrisate 13-acetate (PMA, Sigma) for 24 hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The neuron suspension was then plated on poly-L-lysine and laminin coated 12 mm cover glass (Sigma, Australia) and left to attach for ~3 h at 37 °C ...
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bioRxiv - Systems Biology 2022Quote: ... AP-1-mCherry cells were treated with 100 nM phorbol 12-myristate 13-actetate (PMA, Sigma-Aldrich, catalog # 79346) for 24 hours and was administered at the same time as the doxorubicin ...
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bioRxiv - Cancer Biology 2022Quote: ... tissues from patients prior to chemotherapy were provided for processing (laboratory of HNL) and collected in chilled DMEM/F-12 media (Dulbecco’s Modified Eagle Medium; Millipore-Sigma). Tumor cells were either isolated on the day of collection or the next day (stored in media at 4°C until isolation) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were resolved on 12% SDS–PAGE and proteins were transferred to methanol-activated polyvinylidene fluoride (PVDF) membrane (Millipore) using anode buffer (25 mM Tris HCl pH 10.4 ...
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bioRxiv - Developmental Biology 2020Quote: ... Genomic DNA regions of interest were isolated using 12 ug of antibody against H3K27me3 (Millipore, 07-449, Lot 2653203). Complexes were washed ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 12 mg RBD protein in 3 mL PBS was mixed with equal-volume Freund’s incomplete adjuvant (Sigma-Aldrich) for the second boosting ...
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bioRxiv - Biophysics 2021Quote: ... EMT was induced by incubating cells in medium supplemented with 100 nM 12-O-tetradecanoylphorbol-13-acetate (TPA) (PN:P8139, Sigma) for 48 hours prior to measurement [47] ...
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bioRxiv - Neuroscience 2020Quote: ... 5μg of total cell lysate proteins were resolved on 4-12% SDS-PAGE and transferred onto 0.45 μm polyvinyl difluoride membrane (Millipore) as described above under western blotting of EVs.
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bioRxiv - Immunology 2021Quote: ... PMA (phorbol 12-myristate 13-acetate) (50 ng/mL) and ionomycin (400 ng/mL) (Sigma, St. Louis, Missouri, USA) stimulated hWB were treated with the following compounds AST-VII ...
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bioRxiv - Biophysics 2020Quote: ... and MCF-7 cells (ATCC) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing phenol red-free and DMEM-12 (Sigma-Aldrich), respectively ...
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bioRxiv - Genomics 2020Quote: ... followed by culture on gelatin for 2 weeks in hepato-medium (DMEM:F-12, supplemented with 10% FBS, 1 mg/ml insulin (Sigma), dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2021Quote: ... Purified vimentin wt or Cys328Ser were thawed and subjected to five cycles of dilution with 5 mM Pipes/Na pH 7.0 (10-12 ml) and ultrafiltration on Amicon Ultra-15 units (10 K cutoff; Millipore) for DTT elimination ...
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bioRxiv - Microbiology 2022Quote: ... and equal amounts of cell extracts were separated by SDS-12 % PAGE and transferred onto a nitrocellulose membrane (Sigma). Samples were then analyzed by western blot using the desired antibodies ...
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bioRxiv - Cell Biology 2021Quote: EBs were dissociated as described above and plated on Matrigel-coated 12 well plates at 700,000 cells/well in differentiation medium supplemented with 1 µmol Thiazovivin (Millipore). Medium was removed the following day and replaced with differentiation medium ...
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bioRxiv - Pathology 2021Quote: ... 0.34 mmol/L Na2HPO4, 12 mmol/L NaHCO3, 20 mmol/L HEPES and 1 mmol/L MgCI2; pH 7.4; Sigma) with glucose (0.1% w/v ...
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bioRxiv - Cancer Biology 2021Quote: ... 12-O-Tetradecanoylphorbol 13-acetate (TPA, Cat#P8139) and sodium butyrate (NaB, Cat#AAA1107906) were purchased from Sigma-Aldrich and Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Dalhousie University) and MCF10A (ATCC) cells were cultured in Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 (Sigma-Aldrich), supplemented with reagents from Sigma-Aldrich including ...
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bioRxiv - Microbiology 2021Quote: ... processed into single cell suspensions and stimulated with 50 ng/mL of phorbol 12-myristate 13-acetate (PMA, Sigma) and 1 μg of ionomycin (Sigma ...
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bioRxiv - Immunology 2021Quote: ... treated with phorbol 12-myristate 13-acetate (PMA, 2.5 μg/ml) and ionomycin (0.25 ng/ml, both from Sigma-Aldrich). After incubation ...