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Citations for Millipore Sigma :
2451 - 2500 of 5739 citations for Heme Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: We assessed mitochondrial activity using the 3-(4,5-dimethylthiazol-2-yl-)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay (Sigma-Aldrich, Cat# M6494). As a tetrazolium dye ...
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bioRxiv - Bioengineering 2024Quote: ... and Endothelin-1) using a fluorometric MILLIPLEX ® Human Angiogenesis/Growth Factor Magnetic Bead Panel Multiplex Assay (Sigma-Aldrich, Cat # HAGP1MAG). Each plate was run according to the manufacturer’s protocol using 25 µL sample per well.
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bioRxiv - Cancer Biology 2024Quote: ... glutathione levels in biological samples were measured using Ellman’s Reagent and glutathione reductase using Glutathione GSH/GSSG Assay (Sigma-Aldrich, #MAK440) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... 8 µL of 300 µM final concentration of substrate diluted in TVMV protease assay buffer (50 mM Tris/HCl, 100 mM NaCl, and 2 mM DTT, 50 mg mL-1 BSA (Sigma-Aldrich), pH 8.0 ...
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bioRxiv - Physiology 2023Quote: ... Analysis of the muscle damage marker creatine kinase (CK) was quantified using spectrophotometry using a coupled enzyme-linked reaction assay (Sigma-Aldrich). Samples were measured in duplicate ...
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bioRxiv - Synthetic Biology 2022Quote: ... The buffer of the combined flowthrough was exchanged into assay buffer (20 mM sodium phosphate, 20 mM sodium chloride, pH 8) using a G25 (Sigma-Aldrich) column ...
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bioRxiv - Physiology 2023Quote: ... The resulting crude mitochondrial pellet fraction was resuspended in MIB and protein concentration was quantified with Bradford assay (Sigma-Aldrich, B6916) using a spectrophotometer (Infinite 200 Pro ...
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bioRxiv - Biochemistry 2023Quote: Reporter silencing assays were conducted as in ref 12 with the following changes: cells were treated with 1ug ml−1 dox (Sigma-Aldrich), and gates were set based on reference reporter cells cultured in parallel without dox and reference fully silenced reporter cells (See gating scheme in Figure S2).
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bioRxiv - Microbiology 2023Quote: ... aeruginosa cytotoxicity on A549 cells was assayed using a colorimetric assay that detects the number of metabolically active eukaryotic cells able to cleave the MTT tetrazolium salt (Sigma-Aldrich) to the insoluble formazan dye ...
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bioRxiv - Cell Biology 2023Quote: Proximity Ligation Assay (PLA) was performed in MCF10A cells using the DuoLink® in situ Orange detection reagent (DUO92102, Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: LX-2 cells were lysed and proteins were extracted using radioimmunoprecipitation assay buffer supplemented with complete EDTA-free protease inhibitor cocktail (Sigma-Aldrich). After centrifugation of cell lysates at 12,000 x g for 10 min ...
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bioRxiv - Bioengineering 2023Quote: ... Levels of inflammation-associated cytokines were assessed by MILLIPLEX MAP Porcine Cytokine/Chemokine Magnetic Bead Panel – Immunology Multiplex Assay (Millipore Sigma), following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... Samples were raised to a final volume of 50 μL with Triglyceride Assay Buffer (CAS# MAK266, Sigma Aldrich, St. Louis, MO). 2 μL of Lipase was added to each sample and was incubated for 20 minutes at room temperature to convert triglyceride to glycerol and fatty acids with the plate shaken manually every ten minutes ...
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bioRxiv - Microbiology 2023Quote: ... supernatant of cells was collected and used for quantitating levels of nitrite and nitrate using a Nitrite/Nitrate Assay (Sigma Aldrich) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... The final part portion of the lysate was used to quantify the collagen content according to a hydroxyproline assay (Sigma-Aldrich). The absorbance was measured at 570 nm and the collagen content in the sample was determined from a hydroxyproline standard.
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bioRxiv - Immunology 2023Quote: ... Infected and mock controls were washed three times in PBS and lysed in radioimmunoprecipitation assay (RIPA) lysis buffer (Millipore 20-188) at 2 x with protease inhibitor for western blot analysis.
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bioRxiv - Cell Biology 2022Quote: ... medium was changed to pre-mixed treatments in lipolysis assay medium: insulin 100 nM (Humulin-R U-100, Lilly) and/or isoproterenol 1 mM (Sigma-Aldrich). Lipolysis was measured over 3 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... The sample was spun down at 16,873 x g for 10 min and supernatant was diluted to 2 mg/ml after quantification by Bradford assay (Sigma Aldrich). The sample was reduced for 30 min by adding DTT to a concentration of 5 mM followed by an alkylation step with acrylamide at 15 mM for 30 min in the dark ...
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bioRxiv - Microbiology 2023Quote: ... For phage dilutions in plaque assays we used an SM buffer supplemented with 10 mM calcium chloride and magnesium sulphate (Sigma-Aldrich). We routinely stored phages as filter-sterilised (0.22 μm ...
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bioRxiv - Molecular Biology 2023Quote: Harvested cells were lysed in radioimmunoprecipitation assay (RIPA) buffer with the addition of protease inhibitor cocktail (Sigma-Aldrich, Cat. No. P83490), briefly sonicated ...
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bioRxiv - Physiology 2023Quote: ... Samples of this amniotic fluid supernatant were submitted to the ONPRC/OHSU Endocrine Technologies Core (ETC) to set up bead-based multiplex assays to analyze multiple cytokine and chemokine biomarkers using the Luminex technology (Millipore Sigma).
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bioRxiv - Immunology 2023Quote: ... Cytokines were measured using the MILLIPLEX® Human CD8+ T Cell Magnetic Bead Panel Premixed 17 Plex-Immunology Multiplex Assay (Millipore) according to the manufacturer’s recommendations and analysis was done using the Bio-Plex Manager 6.1 software based on the standard curve.
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bioRxiv - Cell Biology 2023Quote: ... determined by BCA assay were precleared for 1 hour with Protein G Sepharose 4 Fast Flow beads (GE17-0168-01, Millipore Sigma) at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and seeded at approximately 1 million cells/cm2 onto assay plates coated with poly-L-ornithine 44 µg/mL (Sigma P3655) and fibronectin 1 µg/mL (Sigma F1141 ...
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bioRxiv - Biophysics 2024Quote: ... Spheroids were stained with a 10 ml phenol red free assay medium mixture containing 1 μM ethidium bromide (EtBr, E1510, Sigma Aldrich), and 5 μM calcein AM (C3099 ...
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bioRxiv - Immunology 2024Quote: PFA-fixed tissue sections were incubated with anti-FLAP and anti-dsDNA antibodies overnight at 4 °C in antibody diluent followed by processing according to manufacturer’s instructions for Duolink® proximity ligation assay (Sigma #DUO92101). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... a general HDAC fluorometric deacetylase enzyme assay was used to determine total HDAC activities relative to wild-type U251-MG cells from various HDAC-immunoprecipitates (Sigma CS1010). To each well of a 384-well plate ...
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bioRxiv - Biochemistry 2024Quote: 2-MIBS activity assays were performed by pre-incubating samples of 10 to 200 µM GPP (Sigma Aldrich, Cat # 76532-10MG) in a buffer containing 25 µM purified Sg MT ...
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bioRxiv - Cancer Biology 2024Quote: ... Mitochondrial lysates for the assay were prepared via dilution of the final isolated mitochondrial suspensions in CelLytic M (Sigma-Aldrich; C2978) at a protein concentration of 2mg/ml ...
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bioRxiv - Physiology 2024Quote: ... Assay-specific metabolic compounds were diluted day-of in MA media immediately prior to experiments (FCCP 1μM; Sigma Aldrich cat. C2920).
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bioRxiv - Cell Biology 2024Quote: ... Knockdown efficiency and p130Cas over-expression levels were confirmed by Western blotting of cell lysates in radioimmunoprecipitation assay (RIPA) buffer (25 mM Tris-HCl, pH 7.5 [Sigma-Aldrich], 150 mM NaCl [JT Baker], 1% NP-40 [Sigma-Aldrich] ...
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bioRxiv - Immunology 2024Quote: ... The buffer used for assays with purified IgM and IgG fractions was 1% (w/v) casein in 1 x PBS (Sigma-Aldrich) and IgM was detected using an anti-human IgM antibody directly conjugated to HRP (Jackson ImmunoResearch).
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bioRxiv - Microbiology 2024Quote: 30 mg of total protein as determined by Bradford assay of each sample was combined with 100 μL of ANTI-FLAG M2 Affinity Gel (Sigma-Aldrich) and mixed by rotation for 2 hours at 4°C ...
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bioRxiv - Microbiology 2024Quote: Dose-dependent effects of mucins on IAV cell-to-cell spread were studied by plaque assay in presence of mucin from porcine stomach type III (M1778, Sigma-Aldrich) in a concentration range of 0-2% (w/v) ...
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bioRxiv - Cell Biology 2024Quote: Enzymatic activity of ABHD2 was investigated by a fluorometric assay using 7- hydroxycoumarinyl arachidonate (7-HCA; Sigma, Cat-No.: U0383-10MG) as substrate ...
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bioRxiv - Plant Biology 2024Quote: ... CTCF = Integrated Density − (Area of selected cell × Mean fluorescence of background readings) NO contents were also evaluated via the colorimetric Griess assay according to manufacturer instructions (Sigma-Aldrich). The oxidation product of NO ...
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bioRxiv - Plant Biology 2024Quote: ... water was removed and replaced with ROS assay solution (100 μM Luminol [Millipore-Sigma], 20 μg/ml horseradish peroxidase [Millipore-Sigma]), with or without immune elicitors ...
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bioRxiv - Cancer Biology 2024Quote: ... and then incubated in 8 mL of RPMI assay medium containing the desired glucose concentration (0 – 11 mM glucose) (Sigma Aldrich), supplemented with 10% dialyzed FBS (Sigma Aldrich) ...
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bioRxiv - Biophysics 2024Quote: The assay buffer for carbonic anhydrase II is PBS (1x, pH 7.4) with 0.03 % Pluronic F-127 (Millipore, Lot-Nr. 3951203). The assay buffer for lysozyme is TRIS buffer (20 mM ...
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bioRxiv - Biochemistry 2024Quote: ... from E. coli O157) and Stx2 (Verotoxin 2, from E. coli O157) holotoxins for cytotoxicity assay were purchased from Sigma-Aldrich, St ...
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bioRxiv - Cancer Biology 2024Quote: ... Ridgeline analysis was done with ExpressAnalyst (68) Class Switch recombination assay Splenic primary B cells were cultured in RPMI 1640 media with 20% FCS (Sigma Aldrich), 5% Medium NCTC-109 (Thermo Fisher) ...
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bioRxiv - Cell Biology 2024Quote: ... Flow chambers were then washed with motility assay buffer supplemented with 1 mg/mL casein and K560-GFP added in the final imaging buffer (motility assay buffer supplemented with an oxygen scavenger system of 45 μg mL−1 glucose catalase (Sigma-Aldrich), and 1.15 mg mL−1 glucose oxidase (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: Hemolymph from 3rd instar larvae was extracted as described previously 54 and the circulating glucose and trehalose were measured with the Glucose HK assay reagent (Sigma; GAHKLJ20) and trehalase from porcine kidney (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... After 72 hrs we performed 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assays using the Roche MTT protocol (Sigma Aldrich Cat#11465007001). After 24 hours light absorption at 570 nm was measured using the MTT plate reader (Wallac EnVision 1.12) ...
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bioRxiv - Neuroscience 2020Quote: ... The TUNEL protocol was performed using a commercial kit (ApopTag® fluorescein in situ apoptosis detection kit, Millipore). Apoptotic cells were detected as localized bright green cells in a blue background (DAPI ...
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bioRxiv - Neuroscience 2024Quote: ... and some of the myelin was labelled with the green-fluorescent cell linker kit (PKH67 labeling kit, Sigma), according to manufacturer’s specifications.
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bioRxiv - Systems Biology 2023Quote: Leptin - Leptin concentrations were determined by radioimmunoassay using a kit from EMD Millipore (Burlington, MA, kit HL-81K).
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bioRxiv - Molecular Biology 2024Quote: ... we used commercially available ELISA kits: hs-CRP Human C-Reactive Protein ELISA Kit (#RAB0096, Sigma-Aldrich, Germany) and Mercodia Insulin ELISA (#10-1113-01 ...
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bioRxiv - Cell Biology 2024Quote: ... we utilized a commercial membrane protein extraction kit - The ProteoExtract® Native Membrane Protein Extraction Kit (EMD Millipore). This extraction method involves sequential lysis steps ...
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bioRxiv - Immunology 2021Quote: ... Gastrin EIA kit was purchased from Sigma-Millipore ...