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Citations for Millipore Sigma :
2251 - 2300 of 5739 citations for Heme Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the dye solution was removed by double washing with PBS and the dye remaining within viable cells was dissolved over 10 min (gentle shaking) using 1 ml of Neutral Red Assay Solubilization Solution (Sigma). The intensity of Neutral Red dye was measured spectrophotometrically at 540 nm using Fluoroskan plate reader (Thermo) ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... PS transport assay was carried out using a Microfluidic Perfusion Platform (ONIX) driven by the interface software ONIX-FG-SW (Millipore). Strains cho1Δ ...
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bioRxiv - Molecular Biology 2021Quote: ... For the detection of biotin-labelled PARylated proteins the same assay was conducted in presence of biotin-NAD+ (Sigma Aldrich) followed by SDS-PAGE and western blot detection with anti-streptavidin HRP antibody (Molecular Probes).
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bioRxiv - Developmental Biology 2020Quote: ... 1 μl of hemolymph was diluted to 50 μl with autoclaved milli-Q water. 100 μl of glucose assay reagent (Cat. # GAGO20) from Sigma was added and the reaction was incubated at 37 °C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... and the blotting signals were chemically visualized with either the nitro-blue tetrazolium/5-bromo-4-chloro-3′-indolyphosphate (NBT/BCIP) chromogenic assay (Sigma) or infrared scanner ...
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bioRxiv - Neuroscience 2022Quote: ... Virgin females and males used in optogenetic assays were raised in darkness at 25°C on food containing 0.2 mM all-trans- Retinal (Sigma-Aldrich) before and after eclosion ...
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bioRxiv - Molecular Biology 2022Quote: ... Western blots of different fractionations during the RNA polymerase immunoprecipitation and polysome gradient fractionation were performed to assay efficiency of flag-tagged RNA polymerase enrichment using the monoclonal Anti-Flag M2 antibody from mouse (F1804, Sigma).
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bioRxiv - Molecular Biology 2020Quote: Cells were lysed on ice in 500 μl of radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich, R0278, Saint Louis, MO, USA) supplemented with phosphatase (1:250 ...
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bioRxiv - Cell Biology 2022Quote: The effect of the drug has been evaluated both through cell viability using the trypan blue viability assay (Sigma-Aldrich) and through mosaic ratio quantification by ddPCR Technology (Bio-rad’s QX100tm/Copy Number Assay ...
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bioRxiv - Microbiology 2020Quote: ... IL-18 and TNFα were measured in serum samples using MILLIPLEX MAP Porcine Cytokine and Chemokine Magnetic Bead Panel - Immunology Multiplex Assay (Merck Millipore) following the manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: Promastigotes were treated with different concentrations of cAMP analogues and PDE inhibitors followed by 2 hours incubation with 100 μM of 5-bromo-2-Deoxyuridine (BrdU) as per standard protocol and the percentage BrdU incorporation was studied by BrdU cell proliferation assay (Millipore). For the determination of number and position of nucleus (N) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was harvested for cytokine detection using MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay (Millipore) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The assay was performed by pre-incubating Aag2 cells in presence of 10 or 20 μg/ml of Cytochalasin D (Sigma) and 30 or 60 μg/ml of Sp600125 (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... After incubation for 15 min at 37 °C the amount of released phosphate was estimated with Malachite Green Phosphate Assay (Sigma), according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2020Quote: ... Hydrolyzed digestates then underwent hydroxyproline assay as described by Cissell et al,(35) in conjunction with a hydroxyproline standard curve (5-35µg/ml; Sigma-Aldrich). Collagen content was inferred from hydroxyproline quantity using hydroxyproline ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were lysed and processed for cell death detection ELISA assay by following the manufacturer’s instruction (#11774425001, Millipore Sigma). At the end of the assay ...
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bioRxiv - Cell Biology 2021Quote: ... were equilibrated in 500 μl of assay buffer (50 mM Tris-HCl, pH 8, 150 mM NaCl, 1% Triton X-100, PhosSTOP (Millipore-Sigma), and cOmplete Protease Inhibitor Cocktail tablet ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were then added to the motility mix (TIRF Assay Buffer supplemented with 5 mM ATP, 5 mM phosphocreatine (#P7936, Sigma), 7 U/ml creatine phosphokinase (#C3755 Sigma) ...
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bioRxiv - Plant Biology 2020Quote: ... thaliana seedlings used in the root-hair growth assays were grown vertically on plates containing 0.25x Murashige and Skoog (MS; Sigma-Aldrich) medium at pH 5.7 supplemented with 0.6% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... Caliper LabChipGX (Caliper Life Sciences, Inc. Waltham, Massachusetts, USA) and Kapa Biosystems qPCR assay (Sigma-Aldrich, St. Louis, Missouri, USA). The library was then sequenced by Illumina Miseq at Michigan State University (Michigan ...
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bioRxiv - Microbiology 2020Quote: ... Protein-protein interactions in situ were visualized using the Duolink® in situ proximity ligation assay (PLA) system (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: Cytokines in bronchoalveolar lavage fluid or PBS used in MTEC LPS exposures were measured using Milliplex assays (Millipore, Billerica, MA) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Aminopeptidase activity was assessed by fluorescence assays using the fluorogenic peptide L-leucine-7-amido-4 methylcoumarin hydrochloride (Sigma L2145) as a substrate as previously described (19) ...
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bioRxiv - Cancer Biology 2020Quote: ... and total protein concentration was determined using the Bradford Assay according to the manufacturer’s instructions (Sigma-Aldrich, St Louis, MO). Immunoprecipitations were carried out by incubating cell lysates with antibodies as indicated for 12 h at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Extraction of RNA from bead sprout assays (described below) using TRIzol was performed after the removal of HDFs with trypsin-EDTA Solution 10X (59418C, Sigma). cDNA was synthesized with iScript Reverse Transcription Supermix (BioRad ...
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bioRxiv - Cell Biology 2021Quote: The glycogen concentration was measured by a colorimetric assay based on the peroxidase sensitive dye 3,3′,5,5′-tetramethylbenzidine (TMB) (Sigma-Aldrich #860336). Glycogen was digested to glucose using amyloglucosidase (AG) ...
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bioRxiv - Plant Biology 2022Quote: AMPK3 and 4 activity was determined using a coupled enzyme assay with pyruvate kinase and lactate dehydrogenase (LDH, Sigma, P0294), measuring the production of NAD+ that results in absorption-decrease at 340 nm ...
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bioRxiv - Bioengineering 2022Quote: ... Aliquots of collected blood were separated into serum and plasma by centrifugation and serum was analyzed for cytokine levels using a bead-based multiplex assay (Milliplex; Millipore) for Luminex ...
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bioRxiv - Biochemistry 2022Quote: Substrate phosphorylation assays with PKD1KD (D665N) were done with 50 nM PKD1KD (D665N) and 0-100 µM Syntide 2 (Sigma).
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bioRxiv - Cancer Biology 2022Quote: ... and protease inhibitor mixture] and IB assays were performed as previously described (39) using antibody against either FLAG (Sigma–Aldrich), HIF-1α ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were centrifuged 15 min at 13200 rpm at 4°C and protein concentration measured by Bradford assay (Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: Antibiotic sensitivity assays were performed in cation-adjusted (CaCl2 10 mM, MgCl2 5 mM) Mueller-Hinton (pH 7.4) medium (CAMH, Sigma-Aldrich). Overnight cultures of A ...
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bioRxiv - Cell Biology 2021Quote: ... Osteogenic differentiation was analyzed by alkaline phosphatase (ALP) staining with Fast Blue assay (naphthol-AS-MSC phosphate and Fast Blue RR, Sigma) in Tris-HCl buffer (pH 8.9 ...
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bioRxiv - Microbiology 2021Quote: ... Cytokine quantification was performed using a Human Cytokine/Chemokine/Growth Factor Panel A 48-Plex Premixed Magnetic Bead Multiplex Assay (Merck Millipore), using the Luminex MAGPIX System in 96-well plate format ...
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bioRxiv - Immunology 2020Quote: ... Respiration profile was assessed in 96XF instrument with Mitostress assay as indicated upon cell treatment with oligomycin (#75351, 2µM, Sigma-Aldrich), FCCP (#C2990 ...
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bioRxiv - Cell Biology 2021Quote: ... The debris were removed from the lysates by centrifugation and protein estimation was performed by Bicinchoninic Acid (BCA) assay (Sigma). Proteins were resolved by loading 10ug of protein on a 10% and 12% SDS-PAGE gel ...
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bioRxiv - Physiology 2020Quote: ... Final triglyceride concentrations were determined using a spectrophotometric assay with a 4:1 Free Glycerol Agent / Triglyceride Agent solution (Sigma Triglyceride and Free-Glycerol reagents ...
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bioRxiv - Neuroscience 2020Quote: Proximity ligation assay was performed with Duolink® In Situ Orange PLA reagents according to the manufacturer’s protocol (Sigma Aldrich).
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bioRxiv - Biochemistry 2020Quote: ... activity embryos were homogenized in an assay buffer composed by KH2PO4 (25 mM, pH 7.4) and MgCl2 (5 mM) containing protease and phosphatase inhibitors (Sigma Aldrich). Total lysates were then quantified using a BCA Protein Assay Kit (Thermo-Scientific) ...
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bioRxiv - Immunology 2022Quote: ... the culture supernatants were collected and Luminex assay was performed according to the manufacturer’s protocol (EMD Millipore, Cat# MCYTOMAG-70K).
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bioRxiv - Cell Biology 2022Quote: Proliferation of UM51-PrePodo cells grown in Proliferation Medium and Advanced RPMI was measured with a resazurin assay (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... assays were performed as previously described (Bruno T, Cancer Cell, 2002) by using anti-acetyl-Histone H3 (Lys27) antibody (Millipore). Equal amounts of precleared chromatin were added to antibody-bound Dynabeads (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2020Quote: ... Cardiovascular risk markers in plasma were measured using MILLIPLEX MAP Human Cardiovascular Disease (CVD) Magnetic Bead Panel (1 to 4) - Cardiovascular Disease Multiplex Assay (HCVD1MAG-67K, HCVD2MAG-67K, HCVD3MAG-67K, HCVD4MAG-67K, Millipore). Experiments were performed by the Stanford Human Immune Monitoring Center (HIMC ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and AChE activity was measured using the standard Ellman assay (Ellman et al., 1961) with 5,5’-Dithiobis (2-nitrobenzoic acid) (DTNB) and acetylthiocholine iodide (ASChI) as substrates (Sigma-Aldrich). All plates contained three blanks with DTNB ...
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bioRxiv - Immunology 2020Quote: ... and 3×105 PBMC per well were seeded with 40 μl assay medium in XF 96-well cell culture microplate coated with poly-D-lysine (Sigma). The plate was centrifuged at 200 x g for 1 sec with no brake ...
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bioRxiv - Plant Biology 2020Quote: 15N-accumulation assay after 15N-nitrate labeling was performed with 15N-labeled KNO3 (99 atom % 15N, Sigma-Aldrich, no. 335134). For 15N-nitrate accumulation assay ...
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bioRxiv - Developmental Biology 2020Quote: HLECs were treated with chemicals as described above and their intracellular permeability was analyzed using In Vitro Vascular Permeability Imaging Assay (Cat# 17-10398, Sigma) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Sensitivity to micafungin was measured at least in triplicate in a polymerization assay using a 96-well multiscreen high-throughput screen filtration system (Millipore) with a final volume of 100 µl ...
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bioRxiv - Physiology 2021Quote: ... an isotonic solution containing luciferase-luciferin (LL) was prepared from a vial of ATP assay mix-lyophilized powder (Sigma-Aldrich) was used ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: Female mice were primed subcutaneously 48 hours before the assay with 0,1ml estrogen (1mg/ml, Sigma E815 in sesame oil) and 4 hours before the assay with 0,1ml progesterone (5mg/ml ...