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Citations for Millipore Sigma :
2601 - 2650 of 5739 citations for Heme Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: The standard assay for ALP activity was carried out in 500 μL of the reaction mixture containing 2 mM pNPP (Sigma-Aldrich, USA) in 0.1 M Tris–HCl buffer ...
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bioRxiv - Immunology 2024Quote: ... MASP-3 and MAP-1 was measured in an ELISA assay where 10 µg/ml recombinant human albumin (rHA) (Sigma-Aldrich, A9731) was coated on Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Then 20 µl of the mix was transferred to a tube containing 10 µl 3x Stop buffer (assay buffer + 100 mM EDTA, 500 µM proteinase K (Sigma-Aldrich, 107393), 1% SDS ...
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bioRxiv - Microbiology 2023Quote: ... Lung homogenates were clarified of debris by centrifugation at 800 xg for 5 minutes then aliquots were frozen at −80°C neat for plaque assay or diluted at a 1 to 4 ratio in TRIzol (Sigma-Aldrich, MA) for RNA isolation and quantitative PCR ...
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Doxorubicin inhibits phosphatidylserine decarboxylase and confers broad-spectrum antifungal activitybioRxiv - Pathology 2023Quote: The Psd enzyme activity assay based on fluorescence was performed with 1,2-diacetyl benzene/β-mercaptoethanol (1,2-DAB/β-ME) (Sigma-Aldrich, Germany), which forms a fluorescent iso-indole-mercaptide conjugate with PE ...
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bioRxiv - Molecular Biology 2022Quote: HEK293T cells (used for drug susceptibility assays) were cultured in plating medium (FreeStyle F17 supplemented with 10% FBS, 4 mM L-glutamine, 1x PenStrep (Sigma, Cat#P4458)) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cytokine levels in PBMC culture supernatants were measured using the MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel-Custom 6 Plex-Immunology Multiplex Assay (Millipore; #HCYTOMAG-60K). Analytes included were the following ...
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bioRxiv - Microbiology 2023Quote: ... The proximity Ligation Assay (PLA) was then performed using the Duolink® In Situ Detection Red as recommended by the manufacturer (Sigma Aldrich). After the PLA reaction was performed ...
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bioRxiv - Genetics 2023Quote: ... The IC90 at day 6 of treatment for each drug was determined by treating the inducible SpCas9 single clone (before gRNA library infection) with a dose curve of each drug in triplicate and taking an MTT assay measurement (Sigma Aldrich 11465007001) on day 6 following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... the entire frozen neocortex of the left hemisphere was grinded by Dounce homogenizer in RIPA buffer (radioimmunoprecipitation assay buffer, 20-188, Millipore, CA, USA) in the presence of protease inhibitors and phosphatase (Roche ...
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bioRxiv - Bioengineering 2023Quote: ... 90 μL of the remaining solution after ALP assay was mixed with 90 μL of digestion buffer (500 mM phosphate buffer, 5 mM L-cystein (Sigma-Aldrich, C1276), 5 mM EDTA (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... 250 ng of pTOPflash (wt-TCF) or pFOPflash (mutated-TCF binding site, negative control) reporter plasmids (TOPflash luciferase reporter assay, Merck Millipore, Darmstadt, Germany) were transfected with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... Supernatants containing soluble proteins fraction were transferred in a new Eppendorf tube of 1.5mL and the total protein concentration was determined by BRADFORD assay (Sigma-Aldrich, ref 23238).
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bioRxiv - Physiology 2023Quote: ... containing 12.5µL of assay buffer (125mM KCl, 20mM HEPES, 2mM MgCl2, 2mM KH2PO4, pH =7.4) supplemented with 40mM succinate (Sigma-Aldrich # S3674-100G), 40mM malate (Sigma-Aldrich #240176-50G) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then treated in triplicates for 24hrs with varying concentrations of rhIL-23 for analysis via a WST-1 proliferation assay using WST-1 reagent (0501594400, Sigma-Aldrich, Canada) and an apoptosis assay using Caspase-Glo® 3/7 reagent (G8091 ...
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bioRxiv - Molecular Biology 2024Quote: ... were transferred to a 96-well plate and overlayed with 120 µL of assay buffer containing DTNB (D8130, Sigma; 2 mg/ 3mL) and 10 U glutathione reductase (G3664 ...
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bioRxiv - Physiology 2024Quote: ... Assay-specific metabolic compounds were diluted day-of in XF DMEM media immediately prior to experiments: Oligomycin 5μM (Sigma Aldrich cat. 495455), Rotenone 2.5μM (Sigma Aldrich cat ...
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bioRxiv - Systems Biology 2024Quote: ... or thawed on ice from frozen preparations and 25 μg from each sample was prepared for glutamate dehydrogenase assay according to manufacturer instructions (Millipore Sigma MAK099). The absorbance at 450 nm was then measured every two minutes for 4 hours on Cytation 5 (Biotek ...
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bioRxiv - Cell Biology 2024Quote: Interactions were then quantified by β-D-galactosidase activity using the o-nitrophenyl– β-D-galactopyranoside (ONPG) assay (48712-M; Sigma Aldrich) according to the published methods (Amberg et al. ...
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bioRxiv - Microbiology 2024Quote: Cation-Adjusted Muller Hinton Broth (Muller Hinton 2, MH2) was used as the culture medium for all assays (22 g/l; Sigma-Aldrich, UK). For assays requiring solid medium ...
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bioRxiv - Synthetic Biology 2024Quote: ... Assays were performed in 96-well F-bottom black plates were covered with Breathe-Easy® sealing membrane (Sigma-Aldrich, Missouri, USA). On a CLARIOstar® Plus plate reader (BMG Labtech ...
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bioRxiv - Cancer Biology 2024Quote: ... the pellets were stained for 20 minutes in 100 µL of assay buffer containing 5 µg/mL propidium iodide (Sigma-Aldrich P4170) and 3 µL of Annexin V-FITC (Biolegend 0640906) ...
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bioRxiv - Physiology 2020Quote: ... and each circulating hormones were determined using a commercially available ELISA kit for total ghrelin (Rat/Mouse Total Ghrelin ELISA kit, EZRGRT-91K, Millipore), and active ghrelin (Rat/Mouse Total Ghrelin ELISA kit ...
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bioRxiv - Cancer Biology 2022Quote: ... EV were labeled with a PKH67 dye labeling kit-green (or PKH26 dye labeling kit-red) following the manufacturer’s instructions (Sigma-Aldrich). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Bacteriophage DNA was isolated using the Phage DNA Isolation Kit (Norgen) and plasmids were isolated with the GenEluteTM Plasmid Miniprep Kit (Sigma). DNA purification from agarose gel or enzymatic reactions was performed with the NucleoSpin Gel and PCR clean up kit (Macherey-Nagel) ...
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bioRxiv - Plant Biology 2024Quote: ... Each construct was transformed into a yeast sucrose invertase (suc2) mutant using a yeast transformation kit following the manufacturer’s instructions (Yeast Transformation Kit, Sigma-Aldrich). The suc2 mutant is unable to secrete the SUC2 protein and thus cannot grow on media containing sucrose (Suc ...
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bioRxiv - Plant Biology 2020Quote: ... using the GenElute Plasmid Miniprep Kit (Sigma-Aldrich). Inserts were sequenced (GATC Services ...
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bioRxiv - Cancer Biology 2021Quote: Cell Counting Kit-8 (CCK-8; Sigma Aldrich) was used to measure cell viability according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... ApopTag Peroxidase In Situ Apoptosis Detection kit (Millipore) was used to detect apoptotic cells in whole mount embryos ...
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bioRxiv - Physiology 2022Quote: EdU Cell Proliferation Kit (Sigma-Aldrich; Buchs, Switzerland) was used according to the manufacturer’s instructions ...
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A robust method for generating, quantifying and testing large amounts of Escherichia coli persistersbioRxiv - Molecular Biology 2020Quote: Diluent C (Sigma, included in the PKH67 kit). Store at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... Luminex Cytokine/Chemokine 41 Plex Kit (EMD Millipore Corporation ...
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bioRxiv - Bioengineering 2020Quote: ... human VEGF ELISA kit (RAB0507-1KT, Sigma Aldrich), glutaraldehyde solution (340855 ...
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bioRxiv - Neuroscience 2020Quote: ... The Duolink In Situ Proximity Ligation kit (Sigma) was used as per the manufacturer’s instructions with fluorophore-conjugated secondary antibody to recognize MAP2 (1:2000 ...
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bioRxiv - Immunology 2020Quote: ... Leukocyte (TRAP) Kit was purchased from Sigma-Aldrich. Giemsa/ MAY- GRÜNWALD Stain solution was purchased from Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitations were performed using EZ-ChIP kit (Millipore) kits overnight at 4°C and contained 50 μl of digested chromatin (2 × 106 cells) ...
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bioRxiv - Microbiology 2020Quote: The BacLight Bacterial Membrane Potential kit from Sigma was used to measure the effect of SCH-79797 treatment on bacterial membrane potential ...
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bioRxiv - Molecular Biology 2020Quote: ... An enhanced chemiluminescence (ECL) detection kit (Merck Millipore) was used to develop the membranes ...
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bioRxiv - Cancer Biology 2020Quote: ... the CelLytic NuCLEAR Extraction Kit (Sigma; NXTRACT-1KT) was used ...
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bioRxiv - Cell Biology 2020Quote: The PLA kit was purchased from Sigma-Aldrich, and the assay was performed according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... the Fontana-Masson Staining Kit (Sigma Aldrich, HT200) was used ...
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bioRxiv - Physiology 2022Quote: ... Insulin quantification was performed with Luminex Kit (Millipore, MILLIPLEX MAP Mouse Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay ...
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bioRxiv - Cell Biology 2022Quote: ... ProteoExtract®Cytoskeleton Enrichment and Isolation Kit (Millipore) was applied to isolation of cytoskeletal and soluble compartments of indicated cells.
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bioRxiv - Molecular Biology 2022Quote: ... A Milliplex Mouse Th17 Luminex kit (EMD Millipore) with analytes IL-1β ...
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bioRxiv - Cancer Biology 2022Quote: ... the standard cell counting kit (CCK8) (Sigma-Aldrich) assay was performed to test the cell viability ...
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bioRxiv - Immunology 2020Quote: PLA was done using Duolink PLA kit (Sigma)in skin sections of naïve and infected WT and BLT1−/− mice or in vitro in peritoneal macrophages ...
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bioRxiv - Microbiology 2020Quote: ... The Sialic Acid Quantitation Kit (Sigma-Aldrich, USA) was used to estimate the content of sialic acids (Neu5Ac as model one ...
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bioRxiv - Microbiology 2021Quote: ... the Duolink PLA fluorescence detection kit’s protocol (Sigma) was followed ...
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bioRxiv - Neuroscience 2020Quote: ... PLA was performed using the duolink kit (Sigma). Rabbit PLAplus and mouse PLAminus probes were used for secondary antibodies along with the “Duolink Detection reagents Red” (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... The Duolink In Situ Proximity Ligation kit (Sigma) was used as per the manufacturer’s instructions ...