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Citations for Millipore Sigma :
2351 - 2400 of 5739 citations for Heme Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Chemotaxis assays were carried out using 24-well Transwell chambers with 5-μm pore size polycarbonate membranes (Sigma, #CLS3421-48EA). B cells (107 cells/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were collected after centrifuging at 4°C for 10 minutes and the protein amounts were determined by BCA assay (Sigma). The proteins were denatured by boiling for 10 minutes in the DTT containing sample buffer and separated by 4-20% Mini-PROTEIN TGX gels (BIO-RAD ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then incubated on a shaker for 10 minutes in radio-immunoprecipitation assay (RIPA) buffer (#R0278, Sigma, UK) and 1% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... were diluted in pre-warmed XF assay media to achieve the following final concentrations in the cell culture well: 1.5 μM Oligomycin A (Sigma, 75351); 1 or 0.5 μM FCCP (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration in the remaining supernatant was assessed using a Bradford reagent assay according to manufacturer’s protocol (Sigma-Aldrich - B6916), and normalized on collected fecal weight and on animal weight.
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bioRxiv - Immunology 2024Quote: ... The migrated neutrophils in the apical compartment were lysed with 0.5% Triton X-100 and neutrophil MPO activity was quantified using a colorimetric assay using citrate buffered 2,2’-azino-bis(3-ethylbenxothiazoline-6-sulfonic acid) diammonium salt (ABTS) solution (Sigma-Aldrich). After 10 min of incubation ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were then washed twice with PBS and processed using Duolink Proximity Ligation Assay (PLA) reagents (In-Situ PLA Probe Anti-Mouse MINUS, Sigma DUO92004 ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were then washed twice with PBS and processed using Duolink Proximity Ligation Assay (PLA) reagents (In-Situ PLA Probe Anti-Mouse MINUS, Sigma DUO92004; In-Situ PLA Probe Anti-Rabbit PLUS, Sigma DUO92002 ...
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bioRxiv - Cancer Biology 2024Quote: ... The tubes were centrifuged at 6,000 rpm and the supernatants were collected for cytokine analysis by bead-based multiplex assay (MILLIPLEX MAP Mouse High Sensitivity T Cell Panel, Millipore) following the manufacturer protocol27.
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bioRxiv - Cell Biology 2024Quote: ... Knockdown efficiency and p130Cas over-expression levels were confirmed by Western blotting of cell lysates in radioimmunoprecipitation assay (RIPA) buffer (25 mM Tris-HCl, pH 7.5 [Sigma-Aldrich] ...
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bioRxiv - Plant Biology 2024Quote: ... the amount of protein in the sample was quantified by BCA assay using the Bicinchoninic acid solution (Sigma-Aldrich B9643) and CuSO₄.5H₂O ...
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bioRxiv - Physiology 2024Quote: ... after which the reaction mixture was used to measure ATP levels via a luciferase/luciferin based assay system according to manufacturer’s protocol (FLAA - Sigma Aldrich).
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bioRxiv - Neuroscience 2024Quote: ... Primers were designed to target Nr3c1 exon 17 using PyroMark Assay Design software and synthesised by Sigma Aldrich (HPLC purified). The reverse primer was 5’ end biotin labelled ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed with PBS-T and 25 μL of detection antibody diluted in assay buffer added to each well: goat anti-human IgG-AP (1:1,000) (Sigma Aldrich) or goat anti-human IgG3-AP (1:1,000 ...
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bioRxiv - Immunology 2024Quote: The purified neutrophils (Ly6+ cells) were lysed in radioimmunoprecipitation assay (RIPA) buffer containing a protease inhibitor cocktail (Sigma-Aldrich, MO) and denatured at 95°C for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Plasma samples from in vivo studies were previously diluted 1:20 while sera from field studies were diluted 1:10 in assay buffer (PBS-1% BSA-0.05% Tween 20, (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2024Quote: ... MCP1 and TNFα protein concertation in WT and KO-Hmox1 mice blood serum was measured by using Luminex (MILLIPLEX MAP Mouse Cytokine/Chemokine Premixed 32 Plex, Mouse Cytokine/Chemokine Magnetic Bead Premixed 32 Plex and the custom assay panel, Millipore). Assays were performed according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Australia) and the viability was assessed by the resazurin-based Alamar Blue assay (#R7017, Sigma-Aldrich, St. Louis, MO, USA) and the florescence intensity in each well was measured after 90 mins using a bottom-reading florescent plate reader (CLARIOstar Plus ...
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bioRxiv - Immunology 2024Quote: ... and perfusate levels of 71 analytes using two separate panels as per the manufacturer’s instructions for use: (1) the Human Cytokine 48-Plex Discovery Assay® (HD48; Millipore MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel Immunology Multiplex Assay ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then diluted to 1 mg/ml and processed by Eve Technologies using the Mouse High Sensitive 18-Plex Discovery Assay according to the manufacturer’s protocol (Millipore Sigma).
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bioRxiv - Bioengineering 2024Quote: ... and polyIC incubation was evaluated using the MTT (3-(4, 5dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). The cells were seeded into 96 well-format cell culture plates at a seeding density of 8ξ103 cells per well ...
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bioRxiv - Neuroscience 2024Quote: Four-day old adults were randomly collected during paralysis assays and stained with 1 mM X-34 (Sigma, Cat # SML1954) 10 mM Tris-HCl pH 8.0 for 2 hours as previously described (Link et al. ...
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bioRxiv - Microbiology 2024Quote: ... the virus titre was determined by plaque assay on MDCK cells as described previously using a final concentration of 1 µg/ml of TPCK trypsin (SIGMA)[22].
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bioRxiv - Plant Biology 2024Quote: ... water was removed and replaced with ROS assay solution (100 μM Luminol [Millipore-Sigma], 20 μg/ml horseradish peroxidase [Millipore-Sigma]), with or without immune elicitors ...
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bioRxiv - Pathology 2024Quote: ... the AI algorithm was validated against a standard low-resolution image-based caspase-3/7 assay in a proof-of-concept toxicity study where BBB organoids were treated with staurosporine (S5921, Sigma) to induce apoptosis27 ...
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bioRxiv - Synthetic Biology 2024Quote: A cytoplasmic membrane depolarization assay was carried out using the membrane potential-sensitive dye 3,3’-dipropylthiadicarbocyanine iodide (DiSC3-5, Sigma 43608). Mid-logarithmic phase S ...
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bioRxiv - Neuroscience 2024Quote: ... or on coverslips (for immunocytochemistry assay) previously coated with poly-D-lysine (0.1 mg/mL) with neuronal plating medium (MEM supplemented (Sigma-Aldrich), 0.6% glucose and 1 mM pyruvic acid ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cytokine and chemokine levels of cell supernatants were measured using a MCYTOMAG-70K Mouse Cytokine Magnetic Multiplex Assay (Millipore Sigma) using a Luminex 200 analyzer instrument (Luminex Corp ...
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bioRxiv - Biochemistry 2024Quote: ... a reaction mixture consisting of 12nM FatA in assay buffer (50mM Tris HCl pH 8.0, 150mM NaCl, 12nM beta-casein (Sigma C6905)) was combined with test compounds in black 384-well microtitre plates (Greiner ...
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bioRxiv - Biophysics 2024Quote: To perform the osmotic-force-extension assay consecutive osmotic shocks were performed using commercial microfluidic plates (BA04, CellASIC, Millipore-Sigma) controlled by the ONIX microfluidic platform ...
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bioRxiv - Biophysics 2024Quote: To perform the osmotic-force-extension assay consecutive osmotic shocks were performed using commercial microfluidic plates (BA04, CellASIC, Millipore-Sigma) controlled by the ONIX microfluidic platform ...
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bioRxiv - Developmental Biology 2024Quote: ... the cartridge was removed from the incubator and each port was loaded as follows: For the mitochondrial stress test assay – 1st injection port – 20mM Oligomycin A (75351, Sigma); 2nd injection – 20uM FCCP (C2920 ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were purified using the kit Gene eluteTM Gel extraction kit (Sigma Aldrich, MA, USA), re-surveyed on Nanodrop® and delivered for Sanger DNA sequenced at Eurofins Genomics (Ebersberg ...
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bioRxiv - Molecular Biology 2023Quote: ... TUNEL staining was performed using a commercial kit (In Situ Cell Death Detection Kit, TMR (Sigma, 12156792910)) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2023Quote: ... osteoclasts were evaluated by TRAP staining using a commercially available kit (Acid Phosphatase TRAP kit, Sigma–Aldrich), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... primary antibodies were removed with PBS and cells were subjected to the Duolink™ Proximity Ligation Assay protocol according to manufacturer instructions (Sigma: DUO92008 ...
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bioRxiv - Cancer Biology 2021Quote: Cell viability was determined using a commercial 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) viability assay (Sigma Aldrich, Rehovot, Israel). That assay was used to determine how highly 4T1 proliferation was stimulated by Norepinephrine (NE ...
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bioRxiv - Genetics 2021Quote: ... Telomere length was measured by two orthogonal methods: 1) qPCR8 and 2) telomere restriction fragment (TRF) analysis using TeloTAGGG Telomere Length Assay (Sigma Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Biophysics 2022Quote: All enzyme activity were determined by measuring the release of phosphate and formation of a green complex formed between malachite green oxalate with phosphomolybdate and using the malachite green assay as previously described (55) with slight modifications and manufacturer specifications (Sigma-Aldrich). 40 μL of M87SpastinA2C and VPS4BS2C in ATPase buffer (20 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... ATP-hydrolysis rates were measured over the first ~2 min by monitoring the loss of absorbance at 340 nm using a coupled NADH–ATP assay (Burton et al., 2001) with 5 mM ATP (Sigma-Aldrich), pyruvate kinase (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: The sections from two biological replicate experiments were stained using a Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay (Millipore S7165), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Guanosine nucleotide hydrolysis was assessed through synthetase assays performed as above using guanosine 5’-β-thio-diphosphate trilithuim salt (GDPβS) as the only nucleotide phosphoacceptor (Millipore Sigma). The reactions were caried out for 60 mins at 37°C and the spots were autoradiographed as reported above.
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bioRxiv - Microbiology 2021Quote: ... moscoviensis was assessed by measuring total cell protein with the enhanced test tube protocol of the BCA protein assay (Sigma-Aldrich).
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bioRxiv - Physiology 2021Quote: ... in maternal serum and placenta was performed with MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel – Immunology Multiplex Assays (MCYTOMAG-70K, Merck Millipore, Germany) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant was assayed for glucose concentration using glucose oxidase/peroxidase colorimetric glucose assay with 45 μg/ml o-dianisdine dihydrochloride (Sigma-Aldrich), and absorbance measured at 450 nm ...
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bioRxiv - Immunology 2021Quote: ... the cells were lysed for 30 minutes on ice in Radioimmunoprecipitation assay (RIPA) buffer (Sigma Aldrich, St. Louis, MO, USA, #R0278) supplemented with cOm-plete™ EDTA-free Protease Inhibitor Cocktail (Sigma Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... The concentration of inorganic phosphate released by hydrolysis of ATP was measured by tracking absorbance at 620 nm of malachite green binding to phosphate ions using the ATPase/GTPase activity colorimetric assay (Sigma-Aldrich) as described (14).
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bioRxiv - Cell Biology 2021Quote: Transferrin uptake assays were performed by serum-starving cells in DMEM/f12 with 0.5% (w/v) BSA (Sigma Life Sciences A9647) for 30 minutes followed by adding fresh DMEM/f12/ BSA with alexa-fluor 647-labeled transferrin at a concentration of 10 micrograms/milliliter (Jackson Immunoresesarch 009-600-050) ...
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bioRxiv - Cell Biology 2021Quote: ... were equilibrated in 500 μl of assay buffer (50 mM Tris-HCl, pH 8, 150 mM NaCl, 1% Triton X-100, PhosSTOP (Millipore-Sigma), and cOmplete Protease Inhibitor Cocktail tablet ...
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bioRxiv - Microbiology 2020Quote: ... J774A.1 cells were maintained in DMEM supplemented with heat inactivated serum and the assay was complemented with 1 μg/mL C3 (Sigma, C2910) or C3b (Sigma ...