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Citations for Millipore Sigma :
2701 - 2750 of 5739 citations for Heme Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The major components of the assay included: 1) rS-RBD coating of plates as target antigen (2ug/ml in Sigma Carbonate-Bicarbonate Buffer); 2 ...
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bioRxiv - Biochemistry 2020Quote: ... Components for solubilisation buffer and ligands for both ThermoFRET stability assay and fluorescent ligand binding experiments were obtained from Sigma-Aldrich (Havervill, UK) and Anatrace (Ohio ...
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bioRxiv - Biochemistry 2020Quote: ... The GnT I assay was subsequently performed in GnT I activity buffer [63] containing 5 mM of UDP-GlcNAc (Sigma-Aldrich Chemie GmbH).
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bioRxiv - Cancer Biology 2021Quote: Cytotoxicity of USP7 inhibitor P5091 and P22077 was determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay (Sigma-Aldrich, St. Louis, MO, USA). Cytotoxicity was assessed after 24 hrs and 48 hrs of inhibitor treatment in 96 well plates.
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bioRxiv - Microbiology 2020Quote: The MILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Premixed 23 Plex – Immunology Multiplex Assay (Millipore, Burlington, MA, USA; #PCYTMG-40K-PX23) was performed on collected plasma samples following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... A portion of the frozen somatosensory cortex\tissue was homogenized in radioimmunoprecipitation assay (RIPA) buffer with protease and phosphatase inhibitors (SigmaFast, S8820, Sigma-Aldrich, USA and PhosStop, 4906837001, Sigma-Aldrich, USA) The protein concentration of the lysates was quantified using a BCA Protein Assay Kit (Thermo Scientific Pierce Biotechnology ...
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bioRxiv - Neuroscience 2021Quote: ... Milliplex magnetic bead panel assays (CCL2, CXCL10, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, and IL-10; Millipore Sigma, Burlington, MA) were used as per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Samples obtained from in vitro proteolysis assay were analyzed by electrophoresis on an 8% SDS-polyacrylamide gel followed by transfer to Immobilon polyvinylidene difluoride membranes (Millipore Co., Bedford, MA). The membranes were blocked using 4% non-fat milk and immunostained with specific mAb ...
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bioRxiv - Cancer Biology 2020Quote: ... or only on the bottom side (for tumor haptotaxis assays) of 8 or 3 μm hanging cell culture inserts (Millipore, MCEP24H48 and MCSP24H48). After washing the filters with PBS ...
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bioRxiv - Pathology 2021Quote: ... ALT was measured in 1 microliter of plasma collected via cardiac puncture or tail vein bleed using a colorimetric assay (Millipore Sigma cat. # MAK052) according to the manufacturer’s instructions.
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... Neutralization assays were performed by pre-incubating cells with anti-GalactosylCeramide antibody (mGalC, 10µg/mL, Millipore 10µg/mL, 30 min at 4°C) or viral inoculum with monoclonal antibody against HIV-1 gp120 (1-2µg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 24h before monitoring cell viability using the MTT assay: cells were incubated at 35°C for 1 h with MTT (Sigma, Cat #M2128-10G). For that purpose ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantification of siderophores produced by AMB-1 was performed using the Chrome Azurol S (CAS) assay (199532, Sigma-Aldrich, St. Louis, Missouri, USA) [47] ...
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bioRxiv - Microbiology 2022Quote: ... IgG assay samples were incubated with 100 μL of pre-washed 50/50 Protein Sepharose A slurry (Sigma-Aldrich, diluted in lysis buffer) for 1 h ...
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bioRxiv - Neuroscience 2022Quote: Total GLP1 protein in brain tissue or in plasma was determined using GLP-1 Total ELISA 96-Well Plate Assay (Sigma-Aldrich, EZGLP1T-36K). The assay was conducted according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... the workflow of this assay involves four main steps: (i) Tissue is harvested and lysed in lysis buffer containing S-Methyl Methanethiosulfonate (MMTS, Sigma, Cat no. 64306) to block free thiol groups (-SH) ...
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bioRxiv - Physiology 2022Quote: Tissue triglyceride levels were measured using an enzymatic triglyceride assay (Glycerol Reagent, Cat# F6428, Triglyceride Reagent, Cat#T2449, Sigma-Aldrich, Oakville, ON, Canada). In a flat bottom 96 well plate ...
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bioRxiv - Physiology 2024Quote: ... [NH3 + NH +]) was determined spectrophotometrically in 25 µL aliquots of flash frozen plasma by enzymatic ammonia assay (Sigma-Aldrich, USA, Catalog number: AA0100). [TAmm] was calculated from the delta absorbance at 340 nm wavelength before and after the addition of the enzyme L-glutamate dehydrogenase ...
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bioRxiv - Immunology 2024Quote: ... IL-31 and IL-33 cytokines with multiplexed-ELISA assay with Luminex 200 Multiplex Bio-Plex 200 System (EMD Millipore, Billerica, MA, USA) using a Milliplex Map kit (EMD Millipore).
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bioRxiv - Microbiology 2023Quote: Proteins were isolated from hPCLS with the radioimmunoprecipitation assay (RIPA) buffer (Thermo, Cat.# 89901) containing a cocktail of protease inhibitors (Sigma, Cat.# P8340-5ML) and phosphatase inhibitors (Fisher ...
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bioRxiv - Genetics 2023Quote: ... exact number recorded per vial for assay read-out) were then exposed to standard medium prepared with 200mM Mifepristone (RU486) from Sigma Aldrich (cat# 856177) and 2.5% w/v FD&C Blue Dye no 1 from Spectrum (cat# FD110 ...
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bioRxiv - Microbiology 2022Quote: ... The precipitates were extracted into 1x SDS sample buffer containing 2-mercaptethanol and subjected to a standard immunoblotting assay using HRP-conjugated anti-HA antibody (H6533; Sigma; 1:3000 dilution) and anti-FLAG antibody (A8592 ...
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bioRxiv - Microbiology 2022Quote: ... Iron concentrations were then normalized to the protein content in each sample using the bicinchoninic acid (BCA) assay (Sigma Aldrich, St. Louis, MO). Quantification was performed from 3 independent cultures for each strain.
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bioRxiv - Evolutionary Biology 2023Quote: ... three times with a sharp metal rod of the same diameter as the calibrated glass micropipettes used for the assays (5 μL, Sigma, product number P0549). Three glass micropipettes per vial were filled with liquid media ...
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bioRxiv - Plant Biology 2023Quote: ... Expression of the plasmid in floral tissue of T2 plants was confirmed by GUS staining assay following the manufacturer’s protocol (Millipore Sigma, Burlington, MA, USA) (Figure S6) ...
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bioRxiv - Microbiology 2023Quote: Protein lysates from luciferase assays were subjected to 8% SDS-PAGE gel and transferred to PVDF membranes (Millipore-Sigma, St. Louis, MO, USA). Membranes were incubated with 5% nonfat milk at room temperature for 15 minutes for blocking ...
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bioRxiv - Microbiology 2023Quote: Protein lysates from luciferase assays were subjected to 8% SDS-PAGE gel and transferred to PVDF membranes (Millipore-Sigma, St. Louis, MO, USA). Membranes were incubated with 5% nonfat milk at room temperature for 15 minutes for blocking ...
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bioRxiv - Cancer Biology 2023Quote: Proximity ligation assays (PLA) were performed according to manufacturer’s protocol for both flow cytometry and in situ confocal microscopy detection (Sigma, DUO94104 and DUO92013 respectively). For flow cytometry ...
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bioRxiv - Physiology 2024Quote: ... Concentrations of plasma TG in the arterial and venous samples were determined enzymatically using a commercially available assay (Sigma-Aldrich, St. Louis, MO) and by calculating an average concentration based on ten replicates.
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bioRxiv - Neuroscience 2024Quote: ... Cells were counted and seeded (400,000 cells/mL for survival assays) on poly-L-lysine treated glasses (MW 350 kDa, Sigma Chemical, St. Louis, MO). The feeding medium was replaced to growth media ...
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bioRxiv - Bioengineering 2024Quote: ... Metabolic and mitochondrial activity of host cells was assessed with a resazurin assay (7-Hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt, Sigma-Aldrich, Overijse, Belgium). Medium was refreshed with 1 mL of 0.01 mg/mL resazurin in DMEM and incubated for 2 h at 37°C ...
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bioRxiv - Genetics 2024Quote: Snap frozen gastrocnemius or spinalis muscles were homogenised in 4% SDS lysis buffer and assessed for total protein concentration using Direct Detect Assay free cards (Millipore, Cat #DDAC00010-GR). Proteins were separated by SDS-PAGE using stain-free precast midi- criterion gels (Biorad) ...
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bioRxiv - Bioengineering 2024Quote: ... The levels of secreted cytokines and chemokines in the collected effluents were measured using the MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel-Immunology Multiplex Assay from Millipore (Cat.# HCYTOMAG-60K), following the manufacturer’s directions ...
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bioRxiv - Immunology 2022Quote: ... the total DNA methylation signature was evaluated in 100 ng of DNA using the commercial kit (Imprint Methylated DNA Quantification kit, Sigma-Aldrich), according to the manufacturer’s recommendations.
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bioRxiv - Physiology 2020Quote: ... according to the manufacturer’s instructions (Mouse Leptin ELISA Kit, 90030; Crystal Chem, Zaandam, Netherlands; Rat/Mouse Insulin ELISA Kit, cat. EZRMI-13K; Merck Millipore). The intra- and interassay coefficients of variation (CVs ...
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bioRxiv - Bioengineering 2020Quote: ... Apoptosis was assessed by DNA fragmentation staining using a commercially available kit (In Situ Cell Death Detection Kit, TMR red, Sigma-Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... revelation was performed using an avidin/biotin complex coupled with peroxidase (ABC kit, Universal Vectastain Kit PK6200) and diaminobenzidine (Sigma Aldrich).
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bioRxiv - Neuroscience 2020Quote: TUNEL was performed using a standard kit according to the manufacturer’s protocol (TMR In Situ Cell Death Detection Kit, Sigma-Aldrich, #12156792910) in combination with nuclear staining using DAPI (10 ug/mL ...
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bioRxiv - Cell Biology 2022Quote: RNA from different cancer cell lines was extracted using RNA isolation kit (Bio Rad) and treated with DNAse using DNAse 1 kit (AMPD1, Sigma-Aldrich). cDNA synthesis was done using a cDNA synthesis kit (Bio Rad) ...
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bioRxiv - Zoology 2020Quote: ... DNA was extracted from individual cysts or juveniles using the “Sigma Extract-N-Amp Kit (XNAT2)” kit (Sigma, St. Louis, MO) according to the protocol reported by Ma et al ...
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bioRxiv - Microbiology 2022Quote: ... Cells were verified using the Promega GenePrint STR kit (DNA Analysis Facility, Duke University) and as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Evolutionary Biology 2024Quote: Random-PCR amplification of the Viral DNA or RNA were respectively achieved with the GenomePlex Complete Whole Genome Amplification (WGA) Kit and the TransPlex Whole Transcriptome Amplification (WTA) Kit (Sigma-Aldrich) according the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... mRNA was isolated and converted into cDNA using a commercially available kit (GenElute Mammalian Total RNA Miniprep Kit; Sigma-Aldrich, USA). Transcript levels were determined by real-time quantitative polymerase chain reaction (QuantStudio 5 ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Cell proliferation was determined with the iClick EdU Imaging Kit (ABP Biosciences) and apoptosis was measured using the ApopTag Peroxidase in Situ Apoptosis Detection Kit (Millipore Sigma), following the manufacturers’ protocols ...
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bioRxiv - Neuroscience 2023Quote: ... 2 x 100% for 30 minutes each) and then embedded in epoxy embedding medium kit (Epon substitute embedding medium kit; 45359, Sigma Aldrich). For this ...
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bioRxiv - Neuroscience 2023Quote: ... All cells were regularly tested for the presence of mycoplasma using the PCR Mycoplasm Test Kit (PromoKine) or the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Bioengineering 2024Quote: ... device effluent collected from the maternal and fetal chambers was measured using the following ELISA kits: Human IL-1β ELISA Kit (RAB0273, Sigma Aldrich), Human IL-6 ELISA Kit (RAB0306 ...
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bioRxiv - Bioengineering 2024Quote: The measurements were conducted by following the manufacture of NADP/NADPH Quantification Kit (MAK038) and NAD/NADH Quantification Kit (MAK037) from Sigma Aldrich. 10 O.D ...
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bioRxiv - Neuroscience 2021Quote: ... at a density of 40,000 cells/well in 24-well plates for EdU incorporation assays or on 35-mm tissue culture dishes coated with poly-L-lysine (Sigma, P1524; 30 μg/mL) at a density 10×106 cells/dish for immunoblotting ...