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Citations for Millipore Sigma :
2551 - 2600 of 5739 citations for Heme Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... and His-Bind Purification kit (Novagen, USA) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: ... FSH (Millipore Pituitary Panel Multiplex kit, RPT86K), and Inhibin A (Inhibin A ELISA kit ...
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bioRxiv - Microbiology 2024Quote: ... and Cytotoxicity detection Kit (LDH) (Sigma-Aldrich) was used to measure lactate dehydrogenase (LDH ...
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bioRxiv - Cell Biology 2024Quote: ... and Duolink Red detection Kit (Sigma-Aldrich) following the recommended manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... and an enhanced chemiluminescence detection kit (Millipore). EF-Tu was detected using a 1:10,000 dilution of mouse anti-EF-Tu antibody (Hycult #HM6010) ...
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bioRxiv - Immunology 2024Quote: ... using calcium phosphate transfection kit (Sigma-Aldrich) in the presence of 25 µM chloroquine (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... followed by AEC staining Kit (Sigma-Aldrich). The numbers of ASCs were quantified using Immunospot S6 Analyzer (Cellular Technology Limited).
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bioRxiv - Neuroscience 2024Quote: ... Subcellular proteome extraction kit (Cat# 539790, Millipore). Antibodies used are as follows ...
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bioRxiv - Neuroscience 2024Quote: ... using the Duolink Probemaker kit (Sigma-Aldrich). “Indirect” PLAs for nitrated hASYN and nitrated NDUFB8 were performed using probes already conjugation to secondary antibodies and provided in a PLA kit (Duolink ...
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bioRxiv - Neuroscience 2024Quote: ... using the DuoLink kit (Sigma-Aldrich, USA). After the PLA procedure ...
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bioRxiv - Neuroscience 2024Quote: Angiotensin II EIA Kit (#RAB0010, Sigma-Aldrich) was performed to detect ang II peptide levels ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclei isolation kit (Sigma-Aldrich; NUC201-1KT), DTT (R0861) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The Plant Spectrum Total RNA kit (Sigma) was used to isolate the RNA from the homogenized specimens ...
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bioRxiv - Cancer Biology 2024Quote: ... the ProteoExtract Protein Precipitation Kit (Sigma Millipore) was used with the following modifications ...
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bioRxiv - Cancer Biology 2024Quote: ... the ProteoExtract Protein Precipitation Kit (Sigma Millipore) was used with the following modifications ...
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bioRxiv - Bioengineering 2024Quote: We used ATP/ADP kits (Sigma-Aldrich) containing D-luciferin ...
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bioRxiv - Genetics 2024Quote: ... Imprint® DNA Modification kit (Sigma-Aldrich), CpGenome Direct Prep Bisulfite Modification kit (Merck Millipore ...
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bioRxiv - Biochemistry 2024Quote: ... respectively with RNA extraction kit (Sigma-Aldrich) followed by cDNA synthesis with oligoT primers (ProtoScript II ...
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bioRxiv - Developmental Biology 2024Quote: ... incubated in equilibration buffer (ApopTag kit; Millipore) for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... Total IgG response was evaluated by enzyme-linked immunosorbent assays (ELISAs) using 1:5000 dilution of HRP conjugated anti mouse secondary antibody (A4416, Sigma-Aldrich, USA).
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bioRxiv - Genomics 2020Quote: For THP-1/MV4;11 competitive proliferation assays 2 independent INTS6 sgRNAs or a control Scrambled (SCR) sgRNA (Sigma Aldrich, Key Resources Table) with BsmBI compatible overhangs were ligated into BsmBI (NEB R0739 ...
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bioRxiv - Synthetic Biology 2020Quote: ... whilst AL-PHA assay plates were centrifuged 500 g for 10 minutes (MPS 1000 Mini PCR Plate Spinner, Sigma-Aldrich, MO, USA) in order to pellet AL-PHA beads ...
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bioRxiv - Biophysics 2021Quote: Wild type and p75NTR KO CGNs were trypsinized and plated at a density of 40 000 (for cell death assay) or 200 000 (for protein collection) cells per coverslip coated with poly-L-lysine (Sigma, Cat: P7280) in a 24-well (Starlab ...
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bioRxiv - Neuroscience 2021Quote: ... and then lysed with 1× radioimmunoprecipitation assay (RIPA) buffer (Cell Signalling Technologies, cat. #9806) and 200 mM phenylmethylsulfonyl fluoride (Sigma, cat. #P7626) with 1× protease inhibitor cocktail (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were restreaked onto SD/-Trp/-Leu and incubated at 30 °C for 2–3 days before performing a colony-lift β-galactosidase assay (X-gal, Sigma-Aldrich) according to the Yeast Protocols Handbook (PT3024-1 ...
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bioRxiv - Plant Biology 2020Quote: ... Detection was carried out by chemiluminescence assays after incubation with an appropriate horseradish peroxidase (HRP)-conjugated secondary antibody (Sigma or Santa Cruz).
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bioRxiv - Microbiology 2020Quote: ... Simultaneous determination of nitrate and nitrite concentrations induced by the bacteria was done by Vanadium assay with the reduction of nitrate to nitrites by Vanadium (III) combined with detection by the acidic Griess reaction (Sigma-Aldrich, USA), as reported in Miranda et al. ...
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bioRxiv - Microbiology 2021Quote: Cell migration and invasion assays were performed using 24-well transwell chambers with filter membranes 12 µm pore size (Millipore Corporation, PIXP01250). Cells were detached with trypsin-EDTA ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse anti-Flag (1:1,000 for western blotting and 2 μg for co-IP assays; F1804; Sigma, St. Louis, MO, USA). Primers and sequence information are provided in Supplementary Table S1.
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bioRxiv - Microbiology 2020Quote: The fluorescent substrate MUC was applied for the Cts1 liquid assay (4-methylumbelliferyl β-D-N,N′,N′′-triacetylchitotrioside hydrate; M5639 Sigma-Aldrich) (Koepke et al. ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Serum was collected from groups of mice and an in–house assay was used in which anti–human IgG Fc (Sigma, Catalog # FAB3700259) was used to capture human IgG (MS-Hu6) ...
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bioRxiv - Biochemistry 2020Quote: ... cell lysates were diluted 10-fold and incubated with substrate (5 mM α-naphthyl phosphate, Sigma-Aldrich N7255, dissolved in assay buffer) at 30°C for 20 min ...
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bioRxiv - Evolutionary Biology 2022Quote: The enzyme assay for the zebrafish Irg1-like was performed by incubating 7 µg recombinant protein with 1 mM cis-aconitate (Sigma A3412-1G) in 300 µl reaction buffer (10 mM HEPES ...
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bioRxiv - Cancer Biology 2022Quote: Cell proliferation was assessed by the colorimetric assay of sulforhodamine B 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate (SRB) (Sigma Aldrich, USA)[25] ...
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bioRxiv - Cell Biology 2022Quote: To evaluate the angiogenic potential of BEC CM a tube formation assay was performed using the HCMEC/D3 line (SCC066 Merck Millipore, Darmstadt, Germany), as described above ...
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bioRxiv - Physiology 2020Quote: ... and then levels of glucose in both duplicates measured by colorimetric assay following the addition of a glucose oxidase reagent (Sigma; GAGO-20). Levels of glycogen in each experimental sample were then calculated by subtracting the glucose measurements of the untreated duplicate from the amyloglucosidase-treated sample ...
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bioRxiv - Physiology 2021Quote: Cortisol concentrations were measured in mouse sera by a solid phase competitive enzyme linked immunosorbent assay (ELISA) (Sigma-Aldrich Co Ltd, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: A MilliPlex MILLIPLEX® MAP Human Cytokine/Chemokine/Growth Factor Panel A-Immunology Multiplex Assay (HCYTA-60K-13, Millipore Sigma, Burlington, Massachusetts) was run on a Luminex 200 Instrument (LX200-XPON-RUO ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Coating solution was then removed by flicking and plates were washed with OptiMEM and blocked with 25 ul/well of assay buffer (1% Ovalbumin (Sigma #A5503-10G) in OptiMEM containing 1X Pen-Strep (Gibco #15140-148)) ...
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bioRxiv - Physiology 2021Quote: ... and then levels of glucose in both duplicates measured by colorimetric assay following the addition of a glucose oxidase reagent (Sigma; GAGO-20). Levels of glycogen in each experimental sample were then calculated by subtracting the glucose measurements of the untreated duplicate from the amyloglucosidase-treated sample ...
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bioRxiv - Immunology 2020Quote: IgG mediated antibody titer of serum samples collected from immunized animals and convalescent patients’ sera were determined by enzyme-linked immunosorbent assay (ELISA)35 using 1:5000 dilution of HRP conjugated anti mouse (A4416, Sigma-Aldrich, USA) and 1:10000 dilution of HRP conjugated anti human secondary antibody (A0170 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and urethane (ethyl carbamate, EC; CAS# 51-79-6) from Sigma (St. Louis, MO). Primer sets were Kras ...
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bioRxiv - Microbiology 2020Quote: ... aureus binding to fibronectin and fibrinogen was determined using a semi-quantitative adherence assay on 96-well tissue culture plates (Sigma-Aldrich, USA). Plates were coated with 100 μl of 0.02% sodium carbonate (pH 9.6 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cytokine levels in PBMC culture supernatants were measured using the MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel-Custom 6 Plex-Immunology Multiplex Assay (Millipore; #HCYTOMAG-60K). Analytes included were the following ...
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bioRxiv - Microbiology 2023Quote: ... The proximity Ligation Assay (PLA) was then performed using the Duolink® In Situ Detection Red as recommended by the manufacturer (Sigma Aldrich). After the PLA reaction was performed ...
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bioRxiv - Microbiology 2023Quote: ... Lung homogenates were clarified of debris by centrifugation at 800 xg for 5 minutes then aliquots were frozen at −80°C neat for plaque assay or diluted at a 1 to 4 ratio in TRIzol (Sigma-Aldrich, MA) for RNA isolation and quantitative PCR ...
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Doxorubicin inhibits phosphatidylserine decarboxylase and confers broad-spectrum antifungal activitybioRxiv - Pathology 2023Quote: The Psd enzyme activity assay based on fluorescence was performed with 1,2-diacetyl benzene/β-mercaptoethanol (1,2-DAB/β-ME) (Sigma-Aldrich, Germany), which forms a fluorescent iso-indole-mercaptide conjugate with PE ...
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bioRxiv - Molecular Biology 2022Quote: HEK293T cells (used for drug susceptibility assays) were cultured in plating medium (FreeStyle F17 supplemented with 10% FBS, 4 mM L-glutamine, 1x PenStrep (Sigma, Cat#P4458)) ...
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bioRxiv - Neuroscience 2023Quote: ... the entire frozen neocortex of the left hemisphere was grinded by Dounce homogenizer in RIPA buffer (radioimmunoprecipitation assay buffer, 20-188, Millipore, CA, USA) in the presence of protease inhibitors and phosphatase (Roche ...
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bioRxiv - Genetics 2023Quote: ... The IC90 at day 6 of treatment for each drug was determined by treating the inducible SpCas9 single clone (before gRNA library infection) with a dose curve of each drug in triplicate and taking an MTT assay measurement (Sigma Aldrich 11465007001) on day 6 following the manufacturer’s instructions ...