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Citations for Millipore Sigma :
2151 - 2200 of 5739 citations for Heme Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... PLA assay was performed using custom labelled antibodies and the Duolink In Situ Detection Reagents Red (Sigma-Aldrich, DUO92008) as described by the manufacturer but with slight modifications ...
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bioRxiv - Systems Biology 2020Quote: ... Metabolic hormones were measured using MILLIPLEX MAP Human Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay (HMHEMAG-34K, Millipore). Cardiovascular risk markers in plasma were measured using MILLIPLEX MAP Human Cardiovascular Disease (CVD ...
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bioRxiv - Pathology 2021Quote: Proximity ligation assay (PLA) was performed according to the manufacturer’s instructions (Duolink In Situ detection reagent-Red, Sigma Aldrich). Briefly ...
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bioRxiv - Physiology 2020Quote: ... the protein content of each sample was quantified using the Bradford assay (B6916, Sigma Aldrich, Saint Louis, MI, USA) with protein standards made from bovine serum albumin (ALB001.25 ...
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bioRxiv - Physiology 2020Quote: ... and 120-minutes thereafter using a colorimetric assay (PGO enzyme preparation and dianisidine dihydrochloride, Sigma-Aldrich, St. Louis, MO). Area under the curve measurements were calculated using the trapezoidal method [32].
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bioRxiv - Physiology 2021Quote: ... complete culture medium was exchanged for pre-warmed Seahorse Assay medium supplemented with 10mM glucose (Sigma-Aldrich, Poole, UK), 1mM sodium pyruvate (Sigma-Aldrich ...
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bioRxiv - Physiology 2021Quote: ... Total protein concentration was determined using the Bradford assay with bicinchoninic acid (BCA) reagent (Millipore Sigma, St. Louis, MO). Total protein (40 μg ...
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bioRxiv - Genomics 2021Quote: ... and solubilized in IB buffer MB and HS fractions were estimated by Bradford assay or Direct Detect spectrophotometer (Millipore). Protein samples were solubilized in Laemmli buffer with 50 mM DTT ...
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bioRxiv - Neuroscience 2021Quote: ... was prepared in USP30 activity assay buffer (50 mM Tris base, pH 7.5, 100 mM NaCl, 0.1 mg/ml BSA (Sigma, A7030), 0.05% Tween 20 ...
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bioRxiv - Pathology 2021Quote: ... of fresh frozen villous placental tissue samples were homogenized into the assay lysis buffer containing 0.1% Triton-X 100 (Sigma) and further processed per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were cultured for 72 h and then cell death was measured using the tetrazolium dye (2,3)-bis-(2-methoxy-4-nitro-5-sulphenyl)-(2H)-terazolium-5-carboxanilide (XTT) assay (Sigma) according to manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: ... Proliferation at corresponding time points was assessed by MTT (Methylthiazolyldiphenyl-tetrazolium bromide) assay according to the manufacturer’s recommendations (Sigma). For each time point ...
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bioRxiv - Cancer Biology 2022Quote: The PDX tissues were lysed in radioimmunoprecipitation assay (RIPA) buffer with protease and phosphatase inhibitor cocktail (Sigma-Aldrich: #P8340). The protein concentrations were calculated by BCA assay ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... as for the “Group two-choice assay” except using a single 3 cm diameter Petri dish containing 0.67% agarose and 150 mM sucrose (Sigma) onto which a non-accessible container covered with a fabric gauze and a perforated cap of a 15 ml Falcon tube (diameter 1 cm ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were assayed for secreted chemokines and cytokines using the Milliplex 38-plex Human Cytokine/Chemokine Assay (Millipore Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: The effect of CAPG depletion on viability of KO and siRNA-treated cells was assessed at several timepoints using a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay (11465007001; Sigma-Aldrich) per manufacturer directions ...
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bioRxiv - Microbiology 2022Quote: ... Cells from these cultures were then used for batch culture growth kinetic assays in Tibbles–Rawling Minimal Medium13,30 (TR medium) containing 0.1% (weight/volume) alginate (Sigma Aldrich). Sterile alginate solution was prepared using nanopure water and filter sterilized using 0.40 μm surfactant-free cellulose acetate filters (Corning) ...
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bioRxiv - Microbiology 2022Quote: ... was propagated in Vero cells and titers were determined by plaque assay on Vero cells using 1% methylcellulose (Sigma)(Stojdl et al. ...
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bioRxiv - Immunology 2022Quote: Cytokines were measured in duplicate plasma samples using an HCYTMAG-60K-PX41 Premixed 41 Plex - Immunology Multiplex Assay (Millipore). The results were subsequently analyzed using GraphPad Prism Version 9.2.0 software (San Diego ...
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bioRxiv - Cancer Biology 2022Quote: ... 500 cells (for proliferation assay) or 1000 cells (for 3D uptake) per 20μl drop containing 4.8mg/ml methylcellulose (Sigma-Aldrich) and 20μg/ml soluble collagen I (BioEngineering ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Plates were washed twice with 100μL/well HBSS prior to addition of 80μL/well assay buffer (HBSS (Sigma H8264) + 0.1% BSA) ...
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bioRxiv - Microbiology 2024Quote: ... ELISA plates were blocked for 1 h in AD before addition of assay samples for 1 h followed by incubation with 1/20,000 dilution Goat anti-human Fc-HRP (Sigma). All steps were conducted at 37°C and separated by three washes with PBS-T (0.05% Tween-20) ...
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bioRxiv - Cancer Biology 2024Quote: Proximity Ligation Assay (PLA) was performed using the Duolink® PLA reagents according to the manufacturer’s instructions (Sigma Aldrich). An EVOS M7000 imaging system (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: To assess localisation immunofluorescence assays were performed as described previously (31) using anti-HA (1:500, anti-rat,Sigma), anti-MYS (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... cell lysates were tumbled for one hour at 4°C with 20uL of RAS assay reagent (Millipore; #14-278).
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bioRxiv - Cell Biology 2023Quote: ... All following steps of the proximity ligation assay were performed according to suppliers’ instructions (Duolink® PLA; Sigma-Aldrich). Tau-tau sensor molecules interaction was assessed using two monoclonal antibodies against the HA tag (mouse ...
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473, H3K4me1: Millipore #07-436 ...
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bioRxiv - Microbiology 2022Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich), as described (60) ...
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bioRxiv - Developmental Biology 2023Quote: ... 1.1.1.27) activities and concentrations of pyruvate and lactate were measured in samples via enzymatic assays (all from Sigma-Aldrich). All assays have been optimized for the current sample types as explained in the previous studies [37,48] and specific protocol settings for each assay can be found in [37] ...
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bioRxiv - Physiology 2023Quote: ... Triacylglycerol concentrations were determined using a spectrophotometric assay with a 4:1 Free Glycerol Agent / Triacylglycerol Agent solution (Sigma Triglyceride and Free-Glycerol reagents ...
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bioRxiv - Neuroscience 2023Quote: Protein lysates were collected from primary human and murine spinal cord astrocytes in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... Experimental evolution and all root colonization assays were performed in half strength Murashige and Skoog (MS) basal medium (2.22 g L−1, Sigma) supplemented with 0.05% glycerol ...
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bioRxiv - Immunology 2023Quote: Mouse sera were diluted 1:100 in an assay buffer of 0.05% PBS-T supplemented with 3% (w/v) BSA (Sigma) and transferred into a 96-well plate ...
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bioRxiv - Microbiology 2023Quote: Cellular cytotoxicity was measured by a colorimetric assay that makes use of thiazolyl blue tetrazolium bromide (MTT: Sigma, USA). Sub-confluent monolayer culture of RAW264.7 ...
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bioRxiv - Molecular Biology 2023Quote: ... The plate was incubated for 4 h at room temperature and the assay then harvested onto a GF/C filter plate (PE) that had been presoaked in 0.3% polyethyleneimine (Sigma) using a Packard Filter Mate Harvester ...
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bioRxiv - Microbiology 2023Quote: The effect of drug treatments on cell viability was assessed using the MTT [3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay (the MTT powder was obtained from Sigma Aldrich). VTN or Huh-7 cells were seeded the day before treatment at a concentration of 7L×L103 cells per well in DMEM supplemented with 10% FBS and 1% Pen/Strep ...
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bioRxiv - Bioengineering 2022Quote: ... Another part of the solution was used to perform a GAG assay with 1,9-dimethylmethylene blue (DMMB) (Sigma-Aldrich) dye ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were then spun at 21,000 g for 15 min at 4°C and protein concentration determined using Bradford assay (Sigma). Approximately 3-4mg of total cell extract was mixed with 20μl of M2-anti-FLAG agarose beads (bed volume ...
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bioRxiv - Microbiology 2023Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich), as described (Pizzato Scomazzon et al. ...
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bioRxiv - Cancer Biology 2023Quote: T98G cells treated with DMSO (vehicle) or CHIR99021 (8.6 μM) for 24 h were harvested in radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitor (Sigma). Proteins were separated by 12% SDS-PAGE according to [29] ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted with 200 µL assay buffer supplemented with 0.6 mg∕mL 3× Flag® peptide (Sigma Aldrich), and analysed by Western blotting.
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bioRxiv - Cancer Biology 2023Quote: ... Raw ECAR and OCR measurements taken by the instrument from individual wells were normalised using crystal violet staining of plates immediately upon completion of the assay as follows: cells were fixed in 4% PFA and stained using 0.5% crystal violet (Sigma) before solubilisation in 2% SDS (Severn Biotech ...
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bioRxiv - Developmental Biology 2024Quote: Proximity ligation assay (PLA) was carried out with Duolink® In Situ Detection Reagents Far Red (Sigma Aldrich, #DUO92013) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The intracellular ATP concentration in each sample was determined after recovery by using a luciferase assay according to the manufacturer’s instructions (Sigma). ATP concentrations were estimated by using a calibration curve (ATP disodium salt ...
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bioRxiv - Immunology 2024Quote: ... D562 cells were maintained in assay medium (Dulbecco’s Modified Eagle Medium with 10% fetal bovine serum, and 1x Pen-Strep (Sigma)) ...
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bioRxiv - Bioengineering 2024Quote: Cell metabolic activity in dense collagen gels was analysed by alamarBlue® assay (Sigma-Aldrich, St. Louis, MO, USA) very week up to day 21 of culture ...
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bioRxiv - Biochemistry 2024Quote: ... The positive control for the glucosylceramide N-deacylase assay was recombinant Sphingolipid ceramide N-deacylase (SCDase; Sigma-Aldrich, S2563).
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bioRxiv - Biochemistry 2024Quote: ... The positive control for the glycolipid N-deacylase assay was recombinant Sphingolipid ceramide N-deacylase (SCDase; Sigma-Aldrich, S2563) (76) ...
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bioRxiv - Immunology 2024Quote: ... and pellets were resuspended in 75 µL radioimmunoprecipitation assay (RIPA) buffer supplemented with 100x protease inhibitor cocktail (Sigma-Aldrich) and lysed through sonication ...
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bioRxiv - Pathology 2024Quote: ... The final volume in this assay was 100 μl in 1× mitochondrial storage buffer (MITOISO2®, Sigma-Aldrich, Germany). Supernatant fractions were obtained by pelleting the mitochondria (16,000 ×g ...