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Citations for Millipore Sigma :
2251 - 2300 of 2317 citations for 8 Fluoranthenemethanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... all media was removed from organoid wells and replaced with 100 µL Maturation media containing Syn-C4A-GFP or Syn-GFP with 8 µg/mL polybrene (EMD Millipore, TR-1003-G). The final virus concentration was 10 MOI ...
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bioRxiv - Molecular Biology 2022Quote: ... After 48 hours of incubation at 37 °C + 5% CO2 the medium was removed by inversion and cell monolayer was fixed with 8% formalin solution (Sigma-Aldrich, catalog #A252549, USA) for one hour and 20 minutes and then revealed with crystal violet 0.04% (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: U-2OS G3BP1/2 KO cells7 seeded in a 6-well plate were infected at a 60-70% confluency with lentivirus expressing mEGFP-GS-G3BP variants (G3BP1, G3BP2A, and G3BP2B, G3BP1V11A, G3BP2BV11A) and co-incubated with 8 µg/mL polybrene (Millipore Sigma, TR-1003-G). Cells were spun down at 1000xg ...
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bioRxiv - Neuroscience 2024Quote: ... CreERT2 expression was activated in young adult mice (6-8 week-old) by administering tamoxifen (Sigma, T5648-1G, 20 mg/ml in corn oil) by subcutaneous injection (100mg/kg ...
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bioRxiv - Immunology 2024Quote: ... mice were deprived of water for 4hrs and orally administered 200 ml of PBS containing 8 mg of FITC-dextran (4000 Da; FD4, Sigma Aldrich, Saint Louis, MO) per 20 g of body weight ...
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bioRxiv - Cell Biology 2023Quote: Titration of lentiviral vectors was performed by transducing HOS cells (ATCC CRL-1543) with serially diluted vector preparations in the presence Polybrene (5-8 µg/mL; Millipore Sigma, Burlington, MA, USA). HOS cells were grown in DMEM (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... on day 3 of activation (Fig. S1A) 250 µl of concentrated virus was preincubated with 8 µg/mL polybrene (EMD Millipore, TR-1003-G) for 30 min on ice ...
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bioRxiv - Bioengineering 2023Quote: ... Confluent monolayers were treated with 8-(4-chlorophenylthio)adenosine 3′,5′-cyclic monophosphate sodium salt (cPT-cAMP; Sigma-Aldrich, cat# C3912, 25-250 µM) Ro 20-1724 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... and the HEK293T cells were transduced with lentivirus encoding the dGFP reporter in the presence of 8 µg/ml polybrene (Millipore Sigma TR-1003-G). Three days after transduction ...
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bioRxiv - Plant Biology 2023Quote: ... One-liter cultures were centrifuged and resuspended in Buffer A (50 mM Tris pH 8, 200 mM NaCl) with protease inhibitor cocktail (Sigma, St. Louis, MO, USA), DNase I (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... HIV infections were established via spinoculation at 1100xg for 90 min at 30C in the presence of 8 µg/mL polybrene (Millipore Sigma #TR-1003-G) as previously described18 ...
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bioRxiv - Physiology 2023Quote: Hepatocytes were isolated from C57BL/6 and SIRT4 KO mice (8–12 weeks old) by perfusing the liver tissue with collagenase type IV (Sigma-Aldrich, catalog no. C5138) containing media as described in Shaw et al ...
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bioRxiv - Genomics 2023Quote: ... The seeded cells were transduced with the viral library by supplementing their media with 8 μg/mL of polybrene (Sigma-Aldrich Inc # TR-1003) and adding a variety of viral volumes ranging from 0 μL to 50 μL prior to centrifugation at 1000 g for 2 hours at 33°C ...
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bioRxiv - Neuroscience 2023Quote: ... striatal neurons were plated at a density of 50K per well onto 24 well plates or 20K per well onto 8 well chamber slides coated with poly-L-lysine (Sigma Aldrich, St. Louis, USA) (100 µg/ml) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we added an equal volume of stop solution (8 M urea, 1 M Tris–HCl [pH 8.0], and a small amount of Blue Dextran [Sigma Chemical, St. Louis, MO, USA]) to stop the reactions ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then spun down at 7,000g for 8 minutes and resuspended in 100 μL secondary hybridization buffer (10% Formamide, 2X SSC, 10% Ficoll PM-400 (Sigma-Aldrich F5415-25 mL)) ...
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bioRxiv - Bioengineering 2023Quote: ... iSMCs were incubated for 30 mins in blocking solution containing 2% BSA (AppliChem, 9048-46-8) and 0.5% Triton™ X-100 (Sigma-Aldrich, 9002-93-1). The blocking solution was then discarded and iSMCs were incubated for 2 hrs with primary antibodies diluted in blocking solution ...
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bioRxiv - Cancer Biology 2023Quote: Transduction of MIA PaCa-2 cells using lentiviruses was done in 1:1 ratio of viral supernatant to media and supplemented with 8 µg/ml Polybrene (Sigma-Aldrich #TR-1003-G). Following an overnight incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... Head and neck cancer cell lines were incubated overnight with media containing lentiviruses supplemented with 8 µg/ml of polybrene (Millipore-Sigma, no. TR-1003-G). Transduced cells were selected with sequential treatment of puromycin (Biogems ...
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bioRxiv - Cell Biology 2023Quote: ... We lysed each pellet in 1% SDS lysis buffer: 1% SDS in 50 mM Tris (pH 8) containing protease inhibitor cocktail (Sigma Aldrich, catalog no. P8849) and 1 mM PMSF ...
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bioRxiv - Cell Biology 2024Quote: ... Each lysate containing the same amount of proteins was separated on 8–12% SDS-polyacrylamide gels (PAGE) and electro-transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA, #IPVH00010). The membrane was incubated at 4°C overnight with the following primary antibodies ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were resuspended in 20 mL NP buffer (50 mM NaH2PO4, 300 mM NaCl, pH 8) with 100 µl 10X Protease Inhibitor Cocktail (Sigma-Aldrich, Merck, Darmstadt, Germany). Resuspended cells were filtered with Miracloth (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Cell cultures grown in 96 well plates were washed with PBS and incubated with a staining solution (5nM TMRM (Sigma-Aldrich #115532-50-8) and Hoechst were diluted in growth media and incubated for 45 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293T cells were transduced with lentivirus encoding the ABE reporter at varying dilutions (1:10, 1:100, 1:500, and 1:1000) in the presence of 8 μg/ml polybrene (Millipore Sigma Cat. #: TR-1003-G). Three days after transduction ...
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bioRxiv - Microbiology 2019Quote: ... Precipitated cells were resuspended in 200 µL of TE-buffer (10 mM Tris HCl, 1 mM EDTA, pH 8) containing lysozyme from chicken egg white (Sigma Aldrich, St. Louis, Missouri, USA) at a concentration of 15 mg mL-1 and incubated for 30 min at ambient temperature with regular mixing ...
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bioRxiv - Genetics 2019Quote: ... the final concentration of Dvvw dsRNA was brought to 1000 ng/μl and 20% Phenol Red (Cat #143-74-8; Sigma-Aldrich, St. Louis, MO, USA) added for coloration ...
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bioRxiv - Neuroscience 2020Quote: (+)-MK-801 hydrogen maleate (CAS Number: 77086-22-7) and D-amphetamine hemisulfate salt (CAS Number: 51-63-8) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All drugs were dissolved in water at the same conditions as the home tanks ...
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bioRxiv - Microbiology 2021Quote: ... Induced spores were harvested at 8 h after the dilution and were purified from the remaining vegetative cells with Histodenz (Sigma-Aldrich, St. Louis, Missouri, USA) density gradient centrifugation (18) ...
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bioRxiv - Cancer Biology 2022Quote: ... both in the presence and absence of oestradiol (Complete medium with 10% FCS +/- 17-ß Oestradiol 1x10-8 M (Sigma Aldrich – cat no E-060)) in parallel ...
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bioRxiv - Plant Biology 2022Quote: ... genotypes were grown on MS medium supplied with or without the indicated concentration of 1-aminocyclopropane-1-carboxylic acid (ACC) (Sigma-Aldrich, CAS 22059-21-8).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... ground and homogenized western corn rootworm larval tissue (3-7 larvae per biological replicate, n=8-11) using the GenElute Universal Total RNA Purification Kit (Sigma-Aldrich, St. Louis, MO, USA). A NanoDrop spectrophotometer (ND-1000 ...
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bioRxiv - Immunology 2019Quote: ... female BALB/c mice (8 weeks old) were sensitized on days 0 and 14 with 20 µg ovalbumin protein (OVA, grade VI, Sigma-Aldrich Chemie GmbH, Munich, Germany) emulsified in 2 mg alum (Imject™ Alum ...
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bioRxiv - Bioengineering 2020Quote: ... The TEV protease was buffer exchanged (50 mM Tris pH 8, 150 mM NaCl) and concentrated in Amicon ultra spin filters (Sigma-Aldrich, Saint Louis, MO, USA) (10.000 MWC ...
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bioRxiv - Pathology 2021Quote: ... were starved 6 hours from Zeitgeber 14 (ZT14) and refeed for 8 hours with the CD and 20% glucose (Sigma-Aldrich, Saint Louis, MO, USA) in the drinking water.
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bioRxiv - Pharmacology and Toxicology 2019Quote: The protein samples were separated by 8% and 12% SDS PAGE and were transferred to PVDF membranes (Merck Millipore, USA, Cat# ISEQ. 00010, LOT#R6PA4145H). The membranes w ere blocked with 5% skim milk for 3 h at 37 °C and were incubated for 14 h at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... Vero E6 monolayers are washed and incubated for 5-6 days at 37 degrees after which plates are scored using the vitality marker WST-8 (colorimetric cell counting kit, Sigma Aldrich, cat 96992-3000TESTS-F). To this end ...
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bioRxiv - Immunology 2021Quote: ... the plate was washed and blocked with 2%BSA in BBT (Borate Buffer Saline pH 8.2, SIGMA Cat# 08059-100TAB-F, 8 mL Tween 20) for 1 hr/RT ...
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bioRxiv - Biophysics 2023Quote: ... We purchased indium tin oxide (ITO) coated-glass slides with dimensions of 25 mm × 25 mm and surface resistivity of 8-12 Ω/sq from Sigma-Aldrich (St. Louis, MO). Artist grade tracing paper (Jack Richeson & Co. ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were diluted to a final concentration of 2 mg/mL with Laemelli sample buffer (188 mM Tris-HCl, 218 mM SDS, 8% glycerol, and 500 μM Bromophenol Blue (B5525, Sigma-Aldrich, St. Louis, MO, USA). An equal amount of protein ...
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bioRxiv - Plant Biology 2023Quote: ... 150 mg/L cefotaxime] containing 20 mg/L hygromycin B and incubated at 26°oC under conditions of 16-h light (150 μmol photons m-2 s-1) and 8-h dark until roots were established and leaves touched the PhytatrayTM II lid (Sigma-Aldrich, St. Louis, MO, USA). Plantlets were then transferred to soil.
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bioRxiv - Plant Biology 2023Quote: ... Proteins were extracted by the addition of an equal volume of ice-cold Tris-buffered phenol pH 8 (Sigma-Aldrich Corp., St Louis, MO, USA). The sample was shaken for 30 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: The cells were isolated after 5 days of treatment with MTH to induce erythroid differentiation by centrifugation at 1200 rpm for 8 min at room temperature and lysed in Tri-reagentTM (Sigma-Aldrich, St. Louis, Missouri, USA) following manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... Head and neck cancer cell lines were incubated overnight with media containing lentiviruses supplemented with 8 µg/ml of polybrene (Millipore-Sigma, no. TR-1003-G). Transduced cells were selected with sequential treatment of puromycin (Biogems ...
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bioRxiv - Neuroscience 2023Quote: ... Aurora) upon delivery of an odor at three dilutions (no dilution, 1:8, and 1:160 in mineral oil, Sigma-Aldrich; 10 presentations per dilution) was recorded ...
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bioRxiv - Molecular Biology 2024Quote: ... and used for transduction of 1E+05 HEK 293T cells in a 24-well plate along with 8 μg/mL polybrene (Sigma Cat No. TR-1003-G) in 500 μL of complete DMEM ...
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bioRxiv - Neuroscience 2024Quote: ... brains were transferred to 0.1 M PBS containing 0.05% sodium azide (PBSA) (Sodium azide ReagentPlus®, S2002-5G, Sigma Aldrich, CAS number: 26628-22-8) and kept at 4◦C for long-term storage ...
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bioRxiv - Genetics 2021Quote: ... then added 8 µL of X-gal (20 mg/mL in DMSO) and 5 µL of chloramphenicol (Sigma-Aldrich # C0378, 34 mg/mL dissolved in ethanol). After time intervals of 0 ...
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bioRxiv - Cancer Biology 2019Quote: ... 6 week-old mice received a single dose (8 μl g-1 body weight) of tamoxifen (25 mg ml-1 in corn oil, Sigma-Aldrich, St. Louis, MO, USA, T5648) by intraperitoneal injection ...
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bioRxiv - Cell Biology 2019Quote: The method of multiple inductions of acute endotoxaemia comprises intraperitoneal administration of 8 consecutive injections (every 3 days) of LPS 10 mg/kg (Lipopolysaccharides from Escherichia coli 026:B6, Sigma-Aldrich®, St. Louis, MO, USA), in saline (NaCl 0,9%) ...
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bioRxiv - Cell Biology 2021Quote: ... 40,000 cells/well for COS-7 cells and 30,000 cells/well for MEFs (Wild-type or Drp1 KO) were seeded into poly-D-lysine-coated 8 well glass chamber (Sigma, P6407-5MG, Cellvis, C8-1-N). The next day ...